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Jamboree Poster
Jamboree Poster

... -Reporter: lacZ, part I732017 (blue/white screening on X-gal) • If DNA is damaged extensively, then transcription of β-gal • Essentially a reporter-gene assay ...
Chapter 1. Fundamental Properties of Genes
Chapter 1. Fundamental Properties of Genes

... In 1902, Sutton and Boveri independently realized that the behavior of genes in Mendelian crosses mimics the movement of chromosomes during meiosis and fertilization. They surmised that the two alleles of each gene correlated with the homologous pair of chromosomes. The equal segregation of alleles ...
(COX2) gene in giant panda, Ailuropoda melanoleuca
(COX2) gene in giant panda, Ailuropoda melanoleuca

... The COX2 sequences obtained herein were consistent with those within the complete mitochondrial genome of giant panda as reported (Peng et al., 2007) and with those deposited in GenBank, as the BLAST results revealed 100% sequence identity. Although some mutations were detected in the COX2 sequences ...
Plumage Genes and Little Else Distinguish the Genomes of
Plumage Genes and Little Else Distinguish the Genomes of

... the genome. We also found that the divergent regions had, on average, higher levels of absolute divergence (e.g., dxy; Figure S3). The disproportionate extent and number of divergence peaks associated with the Z chromosome are likely due to its reduced Ne and recombination rate but also may be due t ...
Detecting HFE Mutations in Human Genomic DNA
Detecting HFE Mutations in Human Genomic DNA

... The purpose of this laboratory is to screen human genomic DNA samples for mutations in the HFE gene that are linked to hereditary hemochromatosis. Students will use PCR to amplify exons 2 and 4 from the HFE gene and look for restriction fragment length polymorphisms associated with the mutations. Th ...
archaeal genetics — the third way - University of Texas Astronomy
archaeal genetics — the third way - University of Texas Astronomy

... inviolable, for LATERAL GENE TRANSFER can lead to conflicting phylogenetic signals when any one archaeal species is examined in isolation12 (BOX 1). According to the complexity hypothesis of Jain and Rivera13, informational genes are less prone to lateral transfer than operational genes, as the form ...
Compressed q-gram Indexing for Highly Repetitive Biological
Compressed q-gram Indexing for Highly Repetitive Biological

Evaluation of the Y-Chromosome Structure
Evaluation of the Y-Chromosome Structure

... Each Y-STR comprises of short sequences generally between 2 – 5 nucleotides in length, such as DYS393 with a repeat motif AGAT and DYS438 with a repeat motif TTTTC for example, and minisatellites of which there are two (Kayser et al. 2004) that comprise of longer sequences generally between 10 - 60 ...
IdentIfIcatIon of SnP mutatIonS In MYBЕ
IdentIfIcatIon of SnP mutatIonS In MYBЕ

... drought, salt, low and high temperatures. The mechanisms of drought stress tolerance in maize are quite complex and involves a signaling network of genes that controls its adaptation to the stress. Recently, many transcription factors (TFs) for tolerance to drought stress have been identified in mai ...
19.1 Somatostatin Was the First Human Peptide Hormone Produced
19.1 Somatostatin Was the First Human Peptide Hormone Produced

... express somatostatin in bacteria, the coding sequence for somatostatin must be inserted next to a bacterial promoter that is contained within a plasmid. Rather than obtaining the gene from the human genome that encodes this 14-amino acid hormone, the researchers took a different approach. As shown b ...
Low X/Y divergence in four pairs of papaya sex
Low X/Y divergence in four pairs of papaya sex

... BAC pair, based on the density of crossing lines representing homologous sequences. The second inversion, which included the first inversion, involving the X BAC sequence from approximately 44 to 150 kb, and the corresponding Yh BAC sequence, from 0 to 110 kb, showed higher levels of sequence conser ...
Divergent evolution of oxidosqualene cyclases in plants
Divergent evolution of oxidosqualene cyclases in plants

... Families of genes for enzymes implicated in plant secondary metabolism (e.g. cytochrome P450s, glycosyltransferases, acyltransferases, prenyltransferases) have commonly expanded, and the different members have acquired new functions by shifting or broadening substrate and ⁄ or product specificity (V ...
Divergent evolution of oxidosqualene cyclases in plants
Divergent evolution of oxidosqualene cyclases in plants

... Families of genes for enzymes implicated in plant secondary metabolism (e.g. cytochrome P450s, glycosyltransferases, acyltransferases, prenyltransferases) have commonly expanded, and the different members have acquired new functions by shifting or broadening substrate and ⁄ or product specificity (V ...
Woolfe, 2005
Woolfe, 2005

... Experimentally showed CNE-transdev gene association CNEs found in clusters, in front of transdev genes CNEs act at large distances from coding sequence The relative order and positions of CNEs are conserved No vertebrate CNEs were found in invertebrates, even though the genes had clear homologs Many ...
Microarrays: The Future of Prenatal Genetic Testing
Microarrays: The Future of Prenatal Genetic Testing

...  Fewer probes, maximal coverage of regions known to have genes with potential to cause problems  Whole genome arrays  More dense coverage of the whole genome ...
STPM 2011 - Soalan-Percubaan-STPM
STPM 2011 - Soalan-Percubaan-STPM

... I There are commonly 4 or S trophic levels. II Energy is transferred f>om the primary producers to the herbivores. III Each trophic level receives less energy than the trophic level below it. IV Primary producers trap approximately 90% of the light energy that reaches the earth. A ...
An Introduction to the Genetics and Molecular Biology of the F S
An Introduction to the Genetics and Molecular Biology of the F S

... DNA than Escherichia coli cells, they share many of the technical advantages that permitted rapid progress in the molecular genetics of prokaryotes and their viruses. Some of the properties that make yeast particularly suitable for biological studies include rapid growth, dispersed cells, the ease o ...
Raven/Johnson Biology 8e Chapter 17 Answers 1. A recombinant
Raven/Johnson Biology 8e Chapter 17 Answers 1. A recombinant

... b. constructed from DNA from different sources c. constructed from novel combinations of DNA from the same source d. produced through mitotic cell division The correct answer is b— A. Answer a is incorrect. Crossing over produces novel combinations of DNA from a single individual. The offspring are ...
The Co-Evolution of Genes and Culture Pedigrees
The Co-Evolution of Genes and Culture Pedigrees

... Tables 1 and 2 show DNA sequences from two short regions of DNA on chromosome 2. Because each individual has two copies of chromosome 2 (one from each parent), each table includes two DNA sequences per person. Individuals are identified by generation number and individual number. For example, Indiv ...
Supplement: Sequence analysis of GMR-dRetMEN2B
Supplement: Sequence analysis of GMR-dRetMEN2B

... AE003683 maps to different sites within AE003683. There may be 2 inserts, one in 1st intron of Dh, the other 92 bp upstream of Crc, or a single P that induced a 10.4 kb deletion to remove Scm, SpdS, the 1st exon of Dh ...
The UCSC Known Genes
The UCSC Known Genes

Biotechnology Webquest
Biotechnology Webquest

... http://www.accessexcellence.org/RC/AB/BC/J_Michael_Bishop.html 12. Discuss some ethical concerns behind the Human Genome Project. http://www.accessexcellence.org/RC/AB/BC/J_Michael_Bishop.html 13. What is an oncogene? C. Altering DNA http://www.accessexcellence.org/RC/AB/BA/Gene_Therapy_Overview.htm ...
Honors Biology Lab Manual
Honors Biology Lab Manual

... How does a cell “read” the chemical message coded in its DNA in the form of specific base sequences? Part of the answer lies with a second molecule in the nucleus of cells called ribonucleic acid (RNA). RNA is similar to DNA in that its molecules are also formed from nucleotides. However, deoxyribos ...
Marker Saturation and Construction of a High
Marker Saturation and Construction of a High

Biology - Unit 8 Teacher Notes DNA and Protein Synthesis
Biology - Unit 8 Teacher Notes DNA and Protein Synthesis

... Essential question: If life is so closely interconnected then why are organisms so diverse? Instructional goals This module will be separated into two different paradigms: I. What is DNA and how does it copy itself? II How does DNA communicate and build proteins? -What happens if the communication g ...
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Genome editing

Genome editing, or genome editing with engineered nucleases (GEEN) is a type of genetic engineering in which DNA is inserted, replaced, or removed from a genome using artificially engineered nucleases, or ""molecular scissors."" The nucleases create specific double-stranded break (DSBs) at desired locations in the genome, and harness the cell’s endogenous mechanisms to repair the induced break by natural processes of homologous recombination (HR) and nonhomologous end-joining (NHEJ). There are currently four families of engineered nucleases being used: Zinc finger nucleases (ZFNs), Transcription Activator-Like Effector Nucleases (TALENs), the CRISPR/Cas system, and engineered meganuclease re-engineered homing endonucleases.It is commonly practiced in genetic analysis that in order to understand the function of a gene or a protein function one interferes with it in a sequence-specific way and monitors its effects on the organism. However, in some organisms it is difficult or impossible to perform site-specific mutagenesis, and therefore more indirect methods have to be used, such as silencing the gene of interest by short RNA interference (siRNA) . Yet gene disruption by siRNA can be variable and incomplete. Genome editing with nucleases such as ZFN is different from siRNA in that the engineered nuclease is able to modify DNA-binding specificity and therefore can in principle cut any targeted position in the genome, and introduce modification of the endogenous sequences for genes that are impossible to specifically target by conventional RNAi. Furthermore, the specificity of ZFNs and TALENs are enhanced as two ZFNs are required in the recognition of their portion of the target and subsequently direct to the neighboring sequences.It was chosen by Nature Methods as the 2011 Method of the Year.
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