Alpha -antitrypsin alleles in patients with ... emphysema, detected by DNA amplification ...
... isoelectric focusing. As any cell can be used, it is ideal for prenatal diagnosis [26]. Among other methods available for specific detection of point-mutations in the AAT genome, ABE et al. [27] have adopted DNA amplification by PCR together with ribonuclease (RNase) A cleavage methodology [27). The ...
... isoelectric focusing. As any cell can be used, it is ideal for prenatal diagnosis [26]. Among other methods available for specific detection of point-mutations in the AAT genome, ABE et al. [27] have adopted DNA amplification by PCR together with ribonuclease (RNase) A cleavage methodology [27). The ...
Characterization of the neurohypophysial hormone gene loci in
... mammals whereas their homologs in chicken, Xenopus and coelacanth (vasotocin and mesotocin) are linked tail-to-head. In contrast, their pufferfish homologs, vasotocin and isotocin, are located on the same strand of DNA with isotocin located upstream of vasotocin and separated by five genes. These di ...
... mammals whereas their homologs in chicken, Xenopus and coelacanth (vasotocin and mesotocin) are linked tail-to-head. In contrast, their pufferfish homologs, vasotocin and isotocin, are located on the same strand of DNA with isotocin located upstream of vasotocin and separated by five genes. These di ...
08_chapter 1
... property under evolutionary pressures. Moreover the actual symmetry levels in biological sequences were found to be lower and more variable than those obtained using statistical models. The phenomenon of strand symmetry has been conSIdered in the article as an outcome of the compound effects of a wi ...
... property under evolutionary pressures. Moreover the actual symmetry levels in biological sequences were found to be lower and more variable than those obtained using statistical models. The phenomenon of strand symmetry has been conSIdered in the article as an outcome of the compound effects of a wi ...
unique features of the plant life cycle and their consequences
... involves the loss of portions of the gene-expression programme at individual loci (or more precisely, their constituent alleles). As a result, both loci are required during the life cycle, because at particular stages only one locus is expressed. For animals, subfunctionalization is invoked to expla ...
... involves the loss of portions of the gene-expression programme at individual loci (or more precisely, their constituent alleles). As a result, both loci are required during the life cycle, because at particular stages only one locus is expressed. For animals, subfunctionalization is invoked to expla ...
Blueprint of Life
... Showed that inherited characteristics are passed down as discrete unit from parents to their offspring. This was shown through experiments with pea plants. Pea plants were used because they can be easily cross-bred, have a short life cycle & both male & female parts are prevent in their flowers. Men ...
... Showed that inherited characteristics are passed down as discrete unit from parents to their offspring. This was shown through experiments with pea plants. Pea plants were used because they can be easily cross-bred, have a short life cycle & both male & female parts are prevent in their flowers. Men ...
Module 8: Horizontal Gene Transfer
... 7. After clicking on the Image in PNG format (bitmap) in Figure 8.14 has been clicked, an unrooted phylogenetic tree similar to the one shown in Figure 8.15 will appear. A concise review of the interpretation of figure 8.14 can be found at: http://epidemic.bio.ed.ac.uk/how_to_read_a_phylogeny. A sum ...
... 7. After clicking on the Image in PNG format (bitmap) in Figure 8.14 has been clicked, an unrooted phylogenetic tree similar to the one shown in Figure 8.15 will appear. A concise review of the interpretation of figure 8.14 can be found at: http://epidemic.bio.ed.ac.uk/how_to_read_a_phylogeny. A sum ...
Chloroplast Targeting, Distribution and Transcriptional Fluctuation of
... In Fig. 3, Southern hybridization analysis was performed under low stringency by the use of 5¢-half and 3¢-half probes, respectively. Both probes were hybridized with a single band in all lanes. The sizes of hybridized bands were well consistent with those estimated from the disclosing sequence data ...
... In Fig. 3, Southern hybridization analysis was performed under low stringency by the use of 5¢-half and 3¢-half probes, respectively. Both probes were hybridized with a single band in all lanes. The sizes of hybridized bands were well consistent with those estimated from the disclosing sequence data ...
Altering gene expression by aminocoumarins: the role of DNA
... mode of action. This antibiotic interacts with two separate pockets of the gyrase enzyme, situated in the GyrA and GyrB subunits [25]. The parental strain responded to novobiocin, clorobiocin and SD8 by downregulation of recA (Figure 1). SD8 is active in strain HG001 nov142 and resulted in the sever ...
... mode of action. This antibiotic interacts with two separate pockets of the gyrase enzyme, situated in the GyrA and GyrB subunits [25]. The parental strain responded to novobiocin, clorobiocin and SD8 by downregulation of recA (Figure 1). SD8 is active in strain HG001 nov142 and resulted in the sever ...
PART II Introducció 53
... segon reordenament més comú que afecta el cromosoma 15 i dóna lloc a un cromosoma 15 extranumerari (Blennow et al., 1995; Huang et al., 1997). S’han identificat duplicacions proximals de 15q en casos d’autisme i individus amb graus variables de retard mental (Cook et al., 1997). A la regió més prox ...
... segon reordenament més comú que afecta el cromosoma 15 i dóna lloc a un cromosoma 15 extranumerari (Blennow et al., 1995; Huang et al., 1997). S’han identificat duplicacions proximals de 15q en casos d’autisme i individus amb graus variables de retard mental (Cook et al., 1997). A la regió més prox ...
Molecular Evolution of the CMT1A-REP Region: A Human
... Results from PCR and FISH analysis suggest that duplication of the distal REP occurred prior to the divergence of humans and chimpanzees but after that of gorillas and humans. Alternatively, the proximal REP may have been present in the genomes of multiple primates and subsequently deleted in all bu ...
... Results from PCR and FISH analysis suggest that duplication of the distal REP occurred prior to the divergence of humans and chimpanzees but after that of gorillas and humans. Alternatively, the proximal REP may have been present in the genomes of multiple primates and subsequently deleted in all bu ...
Evolutionary Origin and Adaptive Function of Meiosis
... Natural bacterial transformation involves the transfer of DNA from one bacterium to another through the surrounding medium. Transformation depends on the expression of numerous bacterial genes whose products appear to be designed to carry out this process (Chen and Dubnau, 2004; Johnsborg et al., 20 ...
... Natural bacterial transformation involves the transfer of DNA from one bacterium to another through the surrounding medium. Transformation depends on the expression of numerous bacterial genes whose products appear to be designed to carry out this process (Chen and Dubnau, 2004; Johnsborg et al., 20 ...
Chapter 15 - HCC Learning Web
... Around 1900, cytologists and geneticists began to see parallels between the behavior of chromosomes and the behavior of Mendel’s factors. Using improved microscopy techniques, cytologists worked out the process of mitosis in 1875 and meiosis in the 1890s. Chromosomes and genes are both present i ...
... Around 1900, cytologists and geneticists began to see parallels between the behavior of chromosomes and the behavior of Mendel’s factors. Using improved microscopy techniques, cytologists worked out the process of mitosis in 1875 and meiosis in the 1890s. Chromosomes and genes are both present i ...
The Inheritance of DNA, Chromosomes, and Genes
... the cell to make certain proteins. These proteins in turn determine the characteristics of each kind of living thing. The large amount of DNA in human cells is organized into 46 packages called chromosomes. Chromosomes are made of DNA and proteins. The protein portion of the chromosome helps to prot ...
... the cell to make certain proteins. These proteins in turn determine the characteristics of each kind of living thing. The large amount of DNA in human cells is organized into 46 packages called chromosomes. Chromosomes are made of DNA and proteins. The protein portion of the chromosome helps to prot ...
Cloning, expression, sequence analysis and
... Characterization of the cloned autolysin. To determine the hydrolytic bond specificity of the cloned autolysin, purified B. subtilis 168 vegetative cell walls were suspended at 5 mg ml-l in 5 ml 20 mMMgCI2. Cell extract (25 pl) from E. coli DH5a with either PUBS1 or pSFP102 was added and the mixture ...
... Characterization of the cloned autolysin. To determine the hydrolytic bond specificity of the cloned autolysin, purified B. subtilis 168 vegetative cell walls were suspended at 5 mg ml-l in 5 ml 20 mMMgCI2. Cell extract (25 pl) from E. coli DH5a with either PUBS1 or pSFP102 was added and the mixture ...
A Dnmt2-like protein mediates DNA methylation in
... discussed controversially for a long period of time. There are several reports that demonstrate the absence of 5methylcytosine from pupal and adult stages of fly development (Patel and Gopinathan, 1987; Tweedie et al., 1999). In addition, it has also been shown that DNA from Drosophila embryos is la ...
... discussed controversially for a long period of time. There are several reports that demonstrate the absence of 5methylcytosine from pupal and adult stages of fly development (Patel and Gopinathan, 1987; Tweedie et al., 1999). In addition, it has also been shown that DNA from Drosophila embryos is la ...
2.4. Sequence databases
... more quickly. Relational databases can be created using a special programming language called structured query language (SQL). The creation of this type of databases can take a great deal of planning during the design phase. After creation of the original database, a new data category can be easily ...
... more quickly. Relational databases can be created using a special programming language called structured query language (SQL). The creation of this type of databases can take a great deal of planning during the design phase. After creation of the original database, a new data category can be easily ...
A combinational theory for maintenance of sex
... Polyploidy and hybridity alter genotype and phenotype in many different ways, which violates the basic assumptions of current hypotheses that lower genotypic variation or higher mutational load of asexual lineages would be disadvantageous, ‘all else being equal’. Polyploidy can mask effects of delet ...
... Polyploidy and hybridity alter genotype and phenotype in many different ways, which violates the basic assumptions of current hypotheses that lower genotypic variation or higher mutational load of asexual lineages would be disadvantageous, ‘all else being equal’. Polyploidy can mask effects of delet ...
"Frontmatter". In: Plant Genomics and Proteomics
... comparable processes of pattern formation and the underlying genes appear to be similar, the actual mechanisms of getting to the end points of development are different. Once again, this reinforces the need to look specifically at the plant processes in order to understand how plants function. One o ...
... comparable processes of pattern formation and the underlying genes appear to be similar, the actual mechanisms of getting to the end points of development are different. Once again, this reinforces the need to look specifically at the plant processes in order to understand how plants function. One o ...
Potato chromosomes IX and XI carry genes for resistance to potato
... scorable PCR marker linked to Gm (Fig. 2, lane 6). Mapping of the PVM resistance gene Rm The screening of the parental DNA of the ‘‘Rm’’ population with 56 ISSR and 185 RAPD primers resulted in detection of 121 (23%) and 485 (32%) polymorphic DNA fragments. However, only one 1,079 bp ISSR product, a ...
... scorable PCR marker linked to Gm (Fig. 2, lane 6). Mapping of the PVM resistance gene Rm The screening of the parental DNA of the ‘‘Rm’’ population with 56 ISSR and 185 RAPD primers resulted in detection of 121 (23%) and 485 (32%) polymorphic DNA fragments. However, only one 1,079 bp ISSR product, a ...
unit-2 genetics of prokaryotes and eukaryotic
... pairs long. By separating and joining double-stranded DNA molecules at specific sites, this type of recombination enables various types of mobile DNA sequences to move about within and between chromosomes. Site-specific recombination was first discovered as the means by which a bacterial virus, bact ...
... pairs long. By separating and joining double-stranded DNA molecules at specific sites, this type of recombination enables various types of mobile DNA sequences to move about within and between chromosomes. Site-specific recombination was first discovered as the means by which a bacterial virus, bact ...
An extreme cytoplasmic bottleneck in the modern European
... di¡erences in crosses between S. tuberosum ssp. tuberosum and S. tuberosum ssp. andigena (Waugh et al. 1990; Maris 1989), suggest that signi¢cant performance improvements may be realized by widening the cytoplasmic genetic base. A high-resolution cytoplasmic marker system to distinguish between di¡e ...
... di¡erences in crosses between S. tuberosum ssp. tuberosum and S. tuberosum ssp. andigena (Waugh et al. 1990; Maris 1989), suggest that signi¢cant performance improvements may be realized by widening the cytoplasmic genetic base. A high-resolution cytoplasmic marker system to distinguish between di¡e ...