Chapter 13 Mutation, DNA Repair, and Recombination
... Mismatch Repair in E. coli Mismatching or mispairing of G and T (DNA polymerase/exonuclease proofreading activity) The A in GATC sequences is methylated subsequent to DNA replication. In newly replicated DNA, the parental strand is methylated, but the new strand is not. This difference allows ...
... Mismatch Repair in E. coli Mismatching or mispairing of G and T (DNA polymerase/exonuclease proofreading activity) The A in GATC sequences is methylated subsequent to DNA replication. In newly replicated DNA, the parental strand is methylated, but the new strand is not. This difference allows ...
Chapter 12 Molecular Genetics
... The discovery that DNA is the genetic code involved many experiments. Real-World Reading Link Do you like to read mystery novels or watch people on television solve crimes? Detectives search for clues that will help them solve the mystery. Geneticists are detectives looking for clues in the mystery ...
... The discovery that DNA is the genetic code involved many experiments. Real-World Reading Link Do you like to read mystery novels or watch people on television solve crimes? Detectives search for clues that will help them solve the mystery. Geneticists are detectives looking for clues in the mystery ...
Practice exam
... Given a suitable reporter gene for the Hunchback promoter that can be modified and put into transgenic flies, how might you alter the system experimentally to separately measure the effects of these two components? ...
... Given a suitable reporter gene for the Hunchback promoter that can be modified and put into transgenic flies, how might you alter the system experimentally to separately measure the effects of these two components? ...
Extrachromosomal DNA Transformationof Caenorhabditis elegans
... foreign DNA sequences in the transformed worm cells were measured by quantitative hybridization analysis. DNA preparations from transformed populations were spotted onto triplicate nitrocellulose filters. The filters were hybridized with 32P-labeled pBR322 DNA, bacteriophage DNA containing a single- ...
... foreign DNA sequences in the transformed worm cells were measured by quantitative hybridization analysis. DNA preparations from transformed populations were spotted onto triplicate nitrocellulose filters. The filters were hybridized with 32P-labeled pBR322 DNA, bacteriophage DNA containing a single- ...
生物信息学主要英文术语及释义
... state of a hid-den Markov model (representing one column of a multiple sequence alignment of proteins), based on prior distributions found in conserved protein domains (blocks). Distance in sequence analysis（序列距离） The number of observed changes in an optimal alignment of two sequences, usually not c ...
... state of a hid-den Markov model (representing one column of a multiple sequence alignment of proteins), based on prior distributions found in conserved protein domains (blocks). Distance in sequence analysis（序列距离） The number of observed changes in an optimal alignment of two sequences, usually not c ...
Database resources of the National Center for Biotechnology
... These databases include DNA and protein sequences derived from several sources (1,3±6), the NCBI taxonomy, genomes, population sets, gene expression data, gene-oriented sequence clusters in UniGene, sequence-tagged sites in UniSTS, genetic variations in dbSNP, protein structures from the Molecular M ...
... These databases include DNA and protein sequences derived from several sources (1,3±6), the NCBI taxonomy, genomes, population sets, gene expression data, gene-oriented sequence clusters in UniGene, sequence-tagged sites in UniSTS, genetic variations in dbSNP, protein structures from the Molecular M ...
De novo DNA cytosine methyltransferase activities in
... to the C-terminal catalytic domain, a large N-terminal domain that is not present in bacterial enzymes (Bestor et al., 1988). Proteolysis of partially purified mammalian DNA methyltransferases results in increased rate of de novo methylation (Adams et al. 1983; Bestor, 1992), proposed to be the cons ...
... to the C-terminal catalytic domain, a large N-terminal domain that is not present in bacterial enzymes (Bestor et al., 1988). Proteolysis of partially purified mammalian DNA methyltransferases results in increased rate of de novo methylation (Adams et al. 1983; Bestor, 1992), proposed to be the cons ...
A simplified subtractive hybridization protocol used to isolate DNA
... Doyle (1990). After precipitation, the DNA pellet was suspended in 40 p1 T E buffer and 4 p l was used in the PCR reactions. The extracts were prepared from healthy and CVCaffected Citrus sinensis adult plants growing in the field, as well as from healthy seedlings. Symptomless citrus seedlings arti ...
... Doyle (1990). After precipitation, the DNA pellet was suspended in 40 p1 T E buffer and 4 p l was used in the PCR reactions. The extracts were prepared from healthy and CVCaffected Citrus sinensis adult plants growing in the field, as well as from healthy seedlings. Symptomless citrus seedlings arti ...
Advanced Gene Mapping in Eukaryotes
... More specifically, all eight spores in an ascus that show first-division segregation of alleles are parental types: The A allele is on the chromosome with a ● centromere, and the a allele is on the chromosome with the centromere. Furthermore, because it is equally likely that the four chromatids i ...
... More specifically, all eight spores in an ascus that show first-division segregation of alleles are parental types: The A allele is on the chromosome with a ● centromere, and the a allele is on the chromosome with the centromere. Furthermore, because it is equally likely that the four chromatids i ...
Community Genome Annotation Training
... researchers, educators and students working on annotation of eukaryotic genomes. Currently, student-submitted annotations are reconciled by trained annotators providing feedback to faculty at the end of the school year. In most cases, the feedback comes too late to benefit individual student. One of ...
... researchers, educators and students working on annotation of eukaryotic genomes. Currently, student-submitted annotations are reconciled by trained annotators providing feedback to faculty at the end of the school year. In most cases, the feedback comes too late to benefit individual student. One of ...
PDF
... CSEs; and univalent CSEs. We can see that the CSEs from the Celera mouse assembly cover slightly more of the human genome than CSEs from MGSCv3 assembly. Although the numbers of univalent CSEs from two mouse assemblies are almost the same (about 415,000), 31,000 univalent CSEs could be identified in ...
... CSEs; and univalent CSEs. We can see that the CSEs from the Celera mouse assembly cover slightly more of the human genome than CSEs from MGSCv3 assembly. Although the numbers of univalent CSEs from two mouse assemblies are almost the same (about 415,000), 31,000 univalent CSEs could be identified in ...
article in press - MRC
... probe cannot readily detect abnormal restriction enzyme fragments. Recently, a novel method for the detection of gene deletions and duplications has been devised: multiplex ligationdependent probe amplification (MLPA) [8]. The method depends on the hybridisation of two short specific oligonucleotide ...
... probe cannot readily detect abnormal restriction enzyme fragments. Recently, a novel method for the detection of gene deletions and duplications has been devised: multiplex ligationdependent probe amplification (MLPA) [8]. The method depends on the hybridisation of two short specific oligonucleotide ...
Genetic Analysis of DNA Replication in Bacteria: DNAB mutants that suppress DNAC Mutations and DNAQ Mutations That Suppress DNAE Mutations in Salmonella typhimurium.
... The bias was introduced by screening portions of the lysate with different dnaC testers. For example, a suppressor active on only one dnaC allele would be missed unless that particular dnaC allele were present in the screening strain. In contrast, a less specific suppressor would be detected with mo ...
... The bias was introduced by screening portions of the lysate with different dnaC testers. For example, a suppressor active on only one dnaC allele would be missed unless that particular dnaC allele were present in the screening strain. In contrast, a less specific suppressor would be detected with mo ...
mutations
... The effects of mutations on genes vary widely. Some have little or no effect; and some produce beneficial variations. Some negatively disrupt gene function. Whether a mutation is negative or beneficial depends on how its DNA changes relative to the organism’s situation. Mutations are often thought o ...
... The effects of mutations on genes vary widely. Some have little or no effect; and some produce beneficial variations. Some negatively disrupt gene function. Whether a mutation is negative or beneficial depends on how its DNA changes relative to the organism’s situation. Mutations are often thought o ...
PDF
... meiosis-specific gene involved in inter-homologous chromosome dependent repair of DNA double stranded breaks (DSBs), was significantly down-regulated in autotetraploid B. rapa, which presumably contributed to abnormal progression during meiosis I. Although certain DEGs associated with RNA helicase, ...
... meiosis-specific gene involved in inter-homologous chromosome dependent repair of DNA double stranded breaks (DSBs), was significantly down-regulated in autotetraploid B. rapa, which presumably contributed to abnormal progression during meiosis I. Although certain DEGs associated with RNA helicase, ...
W0=2, a stable aneuploid derivative of Candida
... (Scherer & Magee, 1990). One is its lack of a sexual cycle; another is its diploid nature. The first precludes the use of classical genetic analysis to study the properties related to virulence and pathogenicity. The second makes molecular genetics much more cumbersome, since mutants made by classic ...
... (Scherer & Magee, 1990). One is its lack of a sexual cycle; another is its diploid nature. The first precludes the use of classical genetic analysis to study the properties related to virulence and pathogenicity. The second makes molecular genetics much more cumbersome, since mutants made by classic ...
Chapter 9: Frontiers of Biotechnology
... as simple as picking up the molecule and cutting it with a pair of scissors. Instead, scientists use enzymes that act as molecular “scissors.” These enzymes, which slice apart DNA, come from many types of bacteria. Bacterial cells, like your cells, can be infected by viruses. As protection against ...
... as simple as picking up the molecule and cutting it with a pair of scissors. Instead, scientists use enzymes that act as molecular “scissors.” These enzymes, which slice apart DNA, come from many types of bacteria. Bacterial cells, like your cells, can be infected by viruses. As protection against ...
Class XII biology Worksheet genetics and evolution
... Polarity of the two strands involved iii) Template strand iv) Terminator gene b) Mention the function of promoter gene in transcription. AI’09 ...
... Polarity of the two strands involved iii) Template strand iv) Terminator gene b) Mention the function of promoter gene in transcription. AI’09 ...
The Maize Genome Poster
... in which scientific and technological progress translates basic discoveries into practical applications. To this end, the genome sequences of maize and other crops are significantly enhancing established breeding efforts. Soon it will be possible to reconstruct and fully understand the genetic basis ...
... in which scientific and technological progress translates basic discoveries into practical applications. To this end, the genome sequences of maize and other crops are significantly enhancing established breeding efforts. Soon it will be possible to reconstruct and fully understand the genetic basis ...
General background text Pharmacogenetics - CYP3A4
... the functionality of a protein (for example the enzyme or the receptor), but also the physical manifestation of a disease. The phenotype is a result of the genotype that a person possesses, the degree of expression of the gene in question and the combination with environmental factors such as co-med ...
... the functionality of a protein (for example the enzyme or the receptor), but also the physical manifestation of a disease. The phenotype is a result of the genotype that a person possesses, the degree of expression of the gene in question and the combination with environmental factors such as co-med ...
Jump to Terms beginning with: A B Ca-Cn Co
... A process by which radioactive materials, often though not exclusively incorporated into cell structures, are located by exposure to a photographic emulsion forming a pattern on the film corresponding to the location of the radioactive compounds within the cell. A technique in which radioactive mole ...
... A process by which radioactive materials, often though not exclusively incorporated into cell structures, are located by exposure to a photographic emulsion forming a pattern on the film corresponding to the location of the radioactive compounds within the cell. A technique in which radioactive mole ...
Saccharopolyspora erythraea that are involved
... Construction and analysis of mutants. Mutant strains of Sac. erythraea were constructed wherein the eryBZV, eryBV, eryBVZ, ery CZZ, eryCZZI, ery CZV, eryCV or ery CVZ genes were disabled by replacement with altered derivatives of the wildtype allele. Typically, subclones of the erythromycin gene clu ...
... Construction and analysis of mutants. Mutant strains of Sac. erythraea were constructed wherein the eryBZV, eryBV, eryBVZ, ery CZZ, eryCZZI, ery CZV, eryCV or ery CVZ genes were disabled by replacement with altered derivatives of the wildtype allele. Typically, subclones of the erythromycin gene clu ...