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Genome organization of Magnaporthe grisea
Genome organization of Magnaporthe grisea

... considerable efforts have been made to understand the genetics and molecular biology of this fungus. Three different genetic maps for this organism have been reported (Romao and Hamer 1992; Skinner et al. 1993; Sweigard et al. 1993). One map, containing 98 RFLP markers, two isoenzymes and the mating ...
ISMB2008PosterManagingGenomicData
ISMB2008PosterManagingGenomicData

... whole genome comparisons. CMap shows comparative maps of any type (genetic, physical, sequence, …). SynView (shown), GBrowse_syn and SynBrowse are GBrowsebased synteny browsers. ...
Chromosome-encoded gene cluster for the
Chromosome-encoded gene cluster for the

... (Bachofer et al., 1975) and Delftia (Kahng et al., 2000; Boon et al., 2001; Liu et al., 2002) are able to degrade aniline and/ or its derivatives. Moreover, plasmid-dependent aniline degradation has been reported in several bacterial strains (Anson & Mackinnon, 1984; Latorre et al., 1984; McClure & ...
Guidelines for Human Gene Nomenclature (1997)
Guidelines for Human Gene Nomenclature (1997)

... should resist renaming them until enough is known about the gene and related genes for a review of the gene group by scientists as community experts. Overall, the effort should be to assign unique symbols to each ‘‘genetic unit’’ within a species and to support stability of nomenclature terms as muc ...
Identification of Mga1, a G‐protein α‐subunit gene involved in
Identification of Mga1, a G‐protein α‐subunit gene involved in

... have been identified (Shimizu et al., 2007; Chen et al., 2008b). Based on the genetic information, a genetic modification method has also been proposed (Fu et al., 2007; Jia et al., 2010). Secondary metabolite production is controlled at an upper hierarchical level by many global mechanisms, in whic ...
Indigenous Peoples - Council for Responsible Genetics
Indigenous Peoples - Council for Responsible Genetics

... The science of human genetics can focus on a broad variety of topics. Some geneticists study traits we inherit from our parents, some study similar traits within a population and differences between populations, some are looking at genetics from a historical perspective to study the history of popul ...
continued
continued

A novel DNA modification by sulphur
A novel DNA modification by sulphur

... DNA modification associated with the Dnd phenotype is governed by the dnd locus ZX1, a dnd– mutant of S. lividans 66, has an approximately 90 kb chromosomal deletion (Zhou et al., 2004) that includes the putative DNA modification (dnd) gene(s). The dnd locus was mapped to one (16C3) of a set of 13 o ...
Detecting a Transposon in Corn
Detecting a Transposon in Corn

... scientists to characterize genes for which no biological role is known. In a process known as transposon mutagenesis, Ac and Ds elements are crossed into a corn strain to produce Ds insertions in genes. The Ac/Ds mutagenesis system also works well in a number of other plants including tobacco, tomat ...
Identification of Vietnamese Coptotermes pest species based on the
Identification of Vietnamese Coptotermes pest species based on the

... for termite classification/identification as well as phylogenetic analysis has provided a reliable method for identifying many termite species (Clément et al., 2001; Szalanski et al., 2003; Szalanski et al., 2006; Austin et al., 2004b). The 16S rRNA gene has been proven to be an accurate, reliable, ...
Mutations
Mutations

... • If a gene is a linear set of nucleotides nucleotides, recombination between homologous chromosomes carrying different mutations within the same gene should generate wild type wild-type. • T4 phage as an experimental system: • Can examine a large number of progeny to detect rare mutation events • C ...
Nuclear genome size and genomic distribution of ribosomal DNA in
Nuclear genome size and genomic distribution of ribosomal DNA in

Molecular Basis of Heredity--ST03 1.2.7
Molecular Basis of Heredity--ST03 1.2.7

... labels to predict/record in their notebooks which foods they think may contain GMOs. Students then conduct an internet search on foods that contain GMOs. In their notebooks they should record how common the GMO versions are found in food (some sites will give percentages) and the website addresses o ...
Influence of industrial contamination on mobile genetic elements
Influence of industrial contamination on mobile genetic elements

... exposure was recently demonstrated to increase the abundance of IncP-1 plasmids (Smalla et al., 2006). Yet, how MGE abundance is affected by environmental conditions and selective pressures is poorly understood and remains to be tested systematically. In this study, we examined the potential contrib ...
A forkhead-domain gene is mutated in a severe speech and
A forkhead-domain gene is mutated in a severe speech and

... To isolate the complete coding region of this candidate gene, we obtained the genomic sequence of NH0563O05 and adjacent BAC clones. Computer-based investigation of these data, using database search tools and gene prediction programs, enabled us to assemble the sequence of a hypothetical 2.5-kilobas ...
Identification of an Arabidopsis thaliana gene for
Identification of an Arabidopsis thaliana gene for

Evolution of Gene Order and Chromosome Number in
Evolution of Gene Order and Chromosome Number in

... that can occur in a duplicated genome is ‘legitimate’. These translocations involve recombination within a pair of paralogous genes derived from genome duplication. They appear to be rare, because the chromosomes of the other species of Saccharomyces sensu stricto (S. paradoxus, S. bayanus, S. pasto ...
Chapter 12
Chapter 12

... Fig. 12.8: (a) Conservation of the sequence of the patched gene in the vinegar fly, Drosophila melanogaster. (b) The epidermal structures found in a wild-type (+) Drosophila embryo. (c) Mutation of the patched gene disrupts formation of the epidermis in the Drosophila embryo. (d–e) Mutation of the ...
- NDLScholarship
- NDLScholarship

... The isolation of the gene responsible for cystic fibrosis has opened up the reality of genetic screening. A simple test of a person's saliva, costing about the price of a newspaper, allows identification of carriers (people who have one healthy and one faulty copy of the gene)-though only with about ...
Phylogeny, taxonomy, and evolution of the endothelin receptor gene
Phylogeny, taxonomy, and evolution of the endothelin receptor gene

... The potential functional importance of the type I and type I and II (I/II) positions was also assessed against whether therians or non-therians were the more slowly evolving group for these rate shift sites. A type I/II position is a site with different evolutionary rates in the two groups, as well ...
Identification of genes that are associated with DNA repeats in
Identification of genes that are associated with DNA repeats in

... Using in silico analysis we studied a novel family of repetitive DNA sequences that is present among both domains of the prokaryotes (Archaea and Bacteria), but absent from eukaryotes or viruses. This family is characterized by direct repeats, varying in size from 21 to 37 bp, interspaced by similar ...
Review Article Viral Bacterial Artificial - diss.fu
Review Article Viral Bacterial Artificial - diss.fu

... insertion of the mini-F sequences into the poxvirus genome can be facilitated by the cellular recombination machinery in mammalian cells [40–43]. However, unlike herpesviruses, poxviruses do not produce a circular form of the virus genome during replication. This poses a major hurdle for the transfe ...
Ten years of gene targeting: targeted mouse mutants, from vector
Ten years of gene targeting: targeted mouse mutants, from vector

... Before the presumed null phenotype of a mutant can be interpreted in a meaningful way, one must assess whether any residual protein is expressed from the targeted locus. As long as coding sequences remain present in the genome, truncated or mutant forms of the protein may still be expressed. In case ...
Significance of bacterial identification by molecular
Significance of bacterial identification by molecular

... PCR is a technique, which uses a DNA polymerase enzyme to make a huge number of copies of virtually any given piece of DNA or gene. It facilitates a short stretch of DNA (usually fewer than 3000 bp) to be amplified by about a million-fold. In practical terms it amplifies enough specific copies to be ...
Reconstruction of a 450-My-old ancestral vertebrate protokaryotype
Reconstruction of a 450-My-old ancestral vertebrate protokaryotype

... considered to be orthologous to the respective human gene if reciprocal BLAST best-hit searches identified them as such in the Ensembl database (Table S1 in supplementary online material). Another 801 genes were added from a third fish species, medaka (Oryzias latipes, OLA) [9]. Table 1 shows the nu ...
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Genome editing

Genome editing, or genome editing with engineered nucleases (GEEN) is a type of genetic engineering in which DNA is inserted, replaced, or removed from a genome using artificially engineered nucleases, or ""molecular scissors."" The nucleases create specific double-stranded break (DSBs) at desired locations in the genome, and harness the cell’s endogenous mechanisms to repair the induced break by natural processes of homologous recombination (HR) and nonhomologous end-joining (NHEJ). There are currently four families of engineered nucleases being used: Zinc finger nucleases (ZFNs), Transcription Activator-Like Effector Nucleases (TALENs), the CRISPR/Cas system, and engineered meganuclease re-engineered homing endonucleases.It is commonly practiced in genetic analysis that in order to understand the function of a gene or a protein function one interferes with it in a sequence-specific way and monitors its effects on the organism. However, in some organisms it is difficult or impossible to perform site-specific mutagenesis, and therefore more indirect methods have to be used, such as silencing the gene of interest by short RNA interference (siRNA) . Yet gene disruption by siRNA can be variable and incomplete. Genome editing with nucleases such as ZFN is different from siRNA in that the engineered nuclease is able to modify DNA-binding specificity and therefore can in principle cut any targeted position in the genome, and introduce modification of the endogenous sequences for genes that are impossible to specifically target by conventional RNAi. Furthermore, the specificity of ZFNs and TALENs are enhanced as two ZFNs are required in the recognition of their portion of the target and subsequently direct to the neighboring sequences.It was chosen by Nature Methods as the 2011 Method of the Year.
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