Acidaminococcus intestini sp. nov., isolated from human clinical
... 2297.03, ADV 6092.03 and ADV 1338.05 were similar to the type strain tested for phenotypic characteristics (morphology, susceptibility profile to special potency discs and enzymic activities on API Rapid ID 32A kit). DAbout 40 % of A. fermentans strains catabolize glucose and the reaction is weak (R ...
... 2297.03, ADV 6092.03 and ADV 1338.05 were similar to the type strain tested for phenotypic characteristics (morphology, susceptibility profile to special potency discs and enzymic activities on API Rapid ID 32A kit). DAbout 40 % of A. fermentans strains catabolize glucose and the reaction is weak (R ...
Bioinformatik - Chair of Computational Biology
... (A) The steady-state operation of the metabolic network is restricted to the region within a cone, defined as the feasible set. The feasible set contains all flux vectors that satisfy the physicochemical constrains. Thus, the feasible set defines the capabilities of the metabolic network. All feasib ...
... (A) The steady-state operation of the metabolic network is restricted to the region within a cone, defined as the feasible set. The feasible set contains all flux vectors that satisfy the physicochemical constrains. Thus, the feasible set defines the capabilities of the metabolic network. All feasib ...
Manual: QuikChange® II XL Site
... The QuikChange II XL kit is used to make point mutations, replace amino acids, and delete or insert single or multiple adjacent amino acids. The QuikChange II XL site-directed mutagenesis method is performed using PfuUltra™ high-fidelity (HF) DNA polymerase** for mutagenic primerdirected replicatio ...
... The QuikChange II XL kit is used to make point mutations, replace amino acids, and delete or insert single or multiple adjacent amino acids. The QuikChange II XL site-directed mutagenesis method is performed using PfuUltra™ high-fidelity (HF) DNA polymerase** for mutagenic primerdirected replicatio ...
High mutation rates in human and ape pseudoautosomal genes
... If recombination is mutagenic by itself, regions of higher recombination might have a higher mutation rate, resulting in elevated intraspecies diversity and between species divergence in such regions. Drosophila and human single nucleotide polymorphism (SNP) variability was shown to positively corre ...
... If recombination is mutagenic by itself, regions of higher recombination might have a higher mutation rate, resulting in elevated intraspecies diversity and between species divergence in such regions. Drosophila and human single nucleotide polymorphism (SNP) variability was shown to positively corre ...
InGen: Dino Genetics Lab
... 5. Give each student 5 wood-colored popsicle sticks – one for each trait represented in their dinosaur. Students should record each trait on one popsicle stick, with one allele on each side. For example: On one stick I would record “C” on one side and “c” on the other side for my dinosaur with dark ...
... 5. Give each student 5 wood-colored popsicle sticks – one for each trait represented in their dinosaur. Students should record each trait on one popsicle stick, with one allele on each side. For example: On one stick I would record “C” on one side and “c” on the other side for my dinosaur with dark ...
InGen: Dino Genetics Lab
... 5. Give each student 5 wood-colored popsicle sticks – one for each trait represented in their dinosaur. Students should record each trait on one popsicle stick, with one allele on each side. For example: On one stick I would record “C” on one side and “c” on the other side for my dinosaur with dark ...
... 5. Give each student 5 wood-colored popsicle sticks – one for each trait represented in their dinosaur. Students should record each trait on one popsicle stick, with one allele on each side. For example: On one stick I would record “C” on one side and “c” on the other side for my dinosaur with dark ...
A selfish origin for recombination
... 1996; Paques and Haber, 1999). Throughout this discussion I will refer to the physical process of interaction and exchange between two DNA molecules as crossing over, and recombination will mean a possible outcome of crossing over (producing recombinant chromosomes), the other possible outcome being ...
... 1996; Paques and Haber, 1999). Throughout this discussion I will refer to the physical process of interaction and exchange between two DNA molecules as crossing over, and recombination will mean a possible outcome of crossing over (producing recombinant chromosomes), the other possible outcome being ...
RecA maintains the integrity of chloroplast DNA molecules in
... nuclear genomes of many organisms, including plants (Lin et al., 2006). Repair and recombination of chloroplast DNA (cpDNA) in Chlamydomonas reinhardtii is suppressed when a dominant-negative version of E. coli RecA is targeted to chloroplasts (Cerutti et al., 1995). The prevalence of chloroplast-ta ...
... nuclear genomes of many organisms, including plants (Lin et al., 2006). Repair and recombination of chloroplast DNA (cpDNA) in Chlamydomonas reinhardtii is suppressed when a dominant-negative version of E. coli RecA is targeted to chloroplasts (Cerutti et al., 1995). The prevalence of chloroplast-ta ...
... Proteinase K is an endolytic protease that cleaves peptide bonds at the carboxylic sides of aliphatic, aromatic or hydrophobic amino acids. The Proteinase K is classified as a serine protease (1). The smallest peptide to be hydrolyzed by this enzyme is a tetrapeptide. Applications • Isolation of gen ...
Proposal Submission Form
... rDNA: involving eukaryotic viruses (not more than 2/3 genome) in cell culture, used with whole plants (low risk work) and associated small animals, arthropods, or generation of transgenic rodents (BSL1), any work not covered in the other categories (most non-pathogenic rDNA work) rDNA: not in organi ...
... rDNA: involving eukaryotic viruses (not more than 2/3 genome) in cell culture, used with whole plants (low risk work) and associated small animals, arthropods, or generation of transgenic rodents (BSL1), any work not covered in the other categories (most non-pathogenic rDNA work) rDNA: not in organi ...
Genome demethylation and imprinting in the endosperm
... important for imprinting establishment [28,29]. On the paternal allele, this region requires methylation for endosperm expression and imprinting. This region on the maternal PHE1 allele is demethylated, and it is thought that this demethylation allows the PRC2 complex to establish maternal allele s ...
... important for imprinting establishment [28,29]. On the paternal allele, this region requires methylation for endosperm expression and imprinting. This region on the maternal PHE1 allele is demethylated, and it is thought that this demethylation allows the PRC2 complex to establish maternal allele s ...
What`s in a Genotype? - CEUR Workshop Proceedings
... sequence elements. Our basis for understanding these sequences is the Sequence Ontology (SO), an OBO Foundry ontology that models structural and functional genomic sequence features and their attributes. The SO views sequences as abstract entities representing a specific linear ordering of monomers, ...
... sequence elements. Our basis for understanding these sequences is the Sequence Ontology (SO), an OBO Foundry ontology that models structural and functional genomic sequence features and their attributes. The SO views sequences as abstract entities representing a specific linear ordering of monomers, ...
Transgenic Plastids in Basic Research and Plant Biotechnology
... to effective inhibition of plastid protein biosynthesis by spectinomycin (an aminoglycoside antibiotic speci®cally blocking translation on prokaryotic-type 70 S ribosomes). A successfully transformed chloroplast expresses the spectinomycin-resistance gene, aadA, thus allowing for continued cell and ...
... to effective inhibition of plastid protein biosynthesis by spectinomycin (an aminoglycoside antibiotic speci®cally blocking translation on prokaryotic-type 70 S ribosomes). A successfully transformed chloroplast expresses the spectinomycin-resistance gene, aadA, thus allowing for continued cell and ...
Unit 3
... a. Cells contain half the number of chromosomes. b. Homologous pairs of chromosomes are pulled to opposite sides of the cell. c. The total amount of DNA is doubled. d. Doubled chromosomes are split apart. 3. A chicken has 78 chromosomes in its body cells. How many chromosomes are in its sex cells? 4 ...
... a. Cells contain half the number of chromosomes. b. Homologous pairs of chromosomes are pulled to opposite sides of the cell. c. The total amount of DNA is doubled. d. Doubled chromosomes are split apart. 3. A chicken has 78 chromosomes in its body cells. How many chromosomes are in its sex cells? 4 ...
The rapidly evolving field of plant centromeres
... differ in their capacity to promote chromosome segregation [11], and several trans-acting factors that affect knob activation have been identified [39]. At least four distinct genetic functions mapping to Abnormal chromosome 10 (Ab10) play a role in the segregation of knobs; in some cases, these hav ...
... differ in their capacity to promote chromosome segregation [11], and several trans-acting factors that affect knob activation have been identified [39]. At least four distinct genetic functions mapping to Abnormal chromosome 10 (Ab10) play a role in the segregation of knobs; in some cases, these hav ...
Genetic characterization of the mitochondrial DNA - (BORA)
... and ND6), cytochrome c oxidase subunits I – III (COI, COII and COIII), two rRNA genes (12S rRNA and 16S rRNA) and 22 tRNAs. Two copies of tRNA-Lys are present in the mtDNA of L. salmonis, while tRNA-Cys was not identified. Both DNA strands contain coding regions in the salmon louse, in contrast to t ...
... and ND6), cytochrome c oxidase subunits I – III (COI, COII and COIII), two rRNA genes (12S rRNA and 16S rRNA) and 22 tRNAs. Two copies of tRNA-Lys are present in the mtDNA of L. salmonis, while tRNA-Cys was not identified. Both DNA strands contain coding regions in the salmon louse, in contrast to t ...
Transformation of Xanthomonas campestris pathovar
... EcoRI-digested cleared lysates, which revealed a single D N A band with the electrophoretic mobility of authentic cleaved pKT230 D N A , and the lysates could be used to transform E. coli to resistance to streptomycin and kanamycin. All optimization experiments were done at least twice and within ex ...
... EcoRI-digested cleared lysates, which revealed a single D N A band with the electrophoretic mobility of authentic cleaved pKT230 D N A , and the lysates could be used to transform E. coli to resistance to streptomycin and kanamycin. All optimization experiments were done at least twice and within ex ...
Transformation of Xanthomonas campestris pathovar
... EcoRI-digested cleared lysates, which revealed a single D N A band with the electrophoretic mobility of authentic cleaved pKT230 D N A , and the lysates could be used to transform E. coli to resistance to streptomycin and kanamycin. All optimization experiments were done at least twice and within ex ...
... EcoRI-digested cleared lysates, which revealed a single D N A band with the electrophoretic mobility of authentic cleaved pKT230 D N A , and the lysates could be used to transform E. coli to resistance to streptomycin and kanamycin. All optimization experiments were done at least twice and within ex ...
RNA-guided gene drives can efficiently bias inheritance in wild yeast
... eukaryotic organisms as predicted. By itself this does not guarantee the success of gene drives in other organisms as the rate of homologous recombination varies between species and is known to be particularly high in yeast. However, the fact that we observed inheritance biasing rates equal to or ex ...
... eukaryotic organisms as predicted. By itself this does not guarantee the success of gene drives in other organisms as the rate of homologous recombination varies between species and is known to be particularly high in yeast. However, the fact that we observed inheritance biasing rates equal to or ex ...
Comparative analysis of peanut NBS‐LRR gene clusters suggests
... produced dotplots between all LRR domains. The dotplots were produced by matched strings from two protein sequences in comparison. A string length of 7 was used to accommodate the reported conserved consensus pattern xxaxaxx, in which a is an aliphatic residue and x is any amino acid. We also predic ...
... produced dotplots between all LRR domains. The dotplots were produced by matched strings from two protein sequences in comparison. A string length of 7 was used to accommodate the reported conserved consensus pattern xxaxaxx, in which a is an aliphatic residue and x is any amino acid. We also predic ...
Drosophila Genetics
... (In(3LR)TM6B, Hu Tb e) that has the following chromosomal numbering 61A87B86C84F-86C84B-84F84B-75C94A-100F92D-87B61A-63B72E-63B72E75C94A-92E100F-100F. The presence of these inversions within the same chromosome prevents homologous recombination. Since most recessive mutations have no phen ...
... (In(3LR)TM6B, Hu Tb e) that has the following chromosomal numbering 61A87B86C84F-86C84B-84F84B-75C94A-100F92D-87B61A-63B72E-63B72E75C94A-92E100F-100F. The presence of these inversions within the same chromosome prevents homologous recombination. Since most recessive mutations have no phen ...
Antisense Transcript and RNA Processing
... was required for viability but could not produce stable atpB transcripts. Based on strand-specific RT-PCR, S1 nuclease protection, and RNA gel blots, evidence was obtained that the PSþ genome stabilizes atpB mRNA by generating an atpB antisense transcript, which attenuates the degradation of the pol ...
... was required for viability but could not produce stable atpB transcripts. Based on strand-specific RT-PCR, S1 nuclease protection, and RNA gel blots, evidence was obtained that the PSþ genome stabilizes atpB mRNA by generating an atpB antisense transcript, which attenuates the degradation of the pol ...
Genetic Diversity of Rhizobium leguminosarum as Revealed
... diversity of Egyptian rhizobia across the studied locations is very low, probably due to the narrow genetic background of Egyptian rhizobia. Key words: Rhizobium leguminosarum ...
... diversity of Egyptian rhizobia across the studied locations is very low, probably due to the narrow genetic background of Egyptian rhizobia. Key words: Rhizobium leguminosarum ...
Detection of Mosaicism by Augmented Exome
... Introduction The contribution of mosaicism to the development of Mendelian disease has been increasingly recognized as techniques sensitive to mosaic detection have been adopted as primary testing strategies. However, routine detection of mosaicism in conventional genome and exome sequencing is hamp ...
... Introduction The contribution of mosaicism to the development of Mendelian disease has been increasingly recognized as techniques sensitive to mosaic detection have been adopted as primary testing strategies. However, routine detection of mosaicism in conventional genome and exome sequencing is hamp ...