• Study Resource
  • Explore
    • Arts & Humanities
    • Business
    • Engineering & Technology
    • Foreign Language
    • History
    • Math
    • Science
    • Social Science

    Top subcategories

    • Advanced Math
    • Algebra
    • Basic Math
    • Calculus
    • Geometry
    • Linear Algebra
    • Pre-Algebra
    • Pre-Calculus
    • Statistics And Probability
    • Trigonometry
    • other →

    Top subcategories

    • Astronomy
    • Astrophysics
    • Biology
    • Chemistry
    • Earth Science
    • Environmental Science
    • Health Science
    • Physics
    • other →

    Top subcategories

    • Anthropology
    • Law
    • Political Science
    • Psychology
    • Sociology
    • other →

    Top subcategories

    • Accounting
    • Economics
    • Finance
    • Management
    • other →

    Top subcategories

    • Aerospace Engineering
    • Bioengineering
    • Chemical Engineering
    • Civil Engineering
    • Computer Science
    • Electrical Engineering
    • Industrial Engineering
    • Mechanical Engineering
    • Web Design
    • other →

    Top subcategories

    • Architecture
    • Communications
    • English
    • Gender Studies
    • Music
    • Performing Arts
    • Philosophy
    • Religious Studies
    • Writing
    • other →

    Top subcategories

    • Ancient History
    • European History
    • US History
    • World History
    • other →

    Top subcategories

    • Croatian
    • Czech
    • Finnish
    • Greek
    • Hindi
    • Japanese
    • Korean
    • Persian
    • Swedish
    • Turkish
    • other →
 
Profile Documents Logout
Upload
Molecular markers in Brassica Rapa
Molecular markers in Brassica Rapa

... BrCRTISO1 of the OC cultivar had many sequence variations, including single nucleotide polymorphisms (SNPs) and insertions and deletions (InDels), compared to that of the YE cultivar. We developed molecular makers for the identification of OC phenotype based on the polymorphic regions within BrCRTIS ...
Bioportal_2010
Bioportal_2010

... Clustering - CDHIT • Fast greedy incremental clustering process. • Sequences are first sorted in order of decreasing length. • The longest one becomes the representative of the first cluster • Then, each remaining sequence is compared to the representatives of existing cluster. ...
A virulence-associated gene microarray: a tool for
A virulence-associated gene microarray: a tool for

... mixture of two target preparations applied to each array. Genomic DNA from strain Mu50 (Kuroda et al., 2001) was labelled with Cy5 and was used as an internal control strain in each of the hybridizations. DNA from the strain under test was labelled with Cy3. Half of the labelled DNA from the control ...
zinc biofortification of cassava tubers
zinc biofortification of cassava tubers

Ds - e-Acharya
Ds - e-Acharya

... RNA intermediate during transposition and can insert at many different positions in the Drosophila chromosome. The transposition of a P element is controlled by repressors encoded by the element. When P elements are mobilized, they produce a syndrome of traits known collectively as hybrid dysgenesis ...
Biology Ch. 12
Biology Ch. 12

... A. intervening sequences in the mRNA molecule called introns B. the original mRNA made in the nucleus called the pre-mRNA C. how the sequence of bases in the mRNA molecule codes for amino acids D. the function of many adenine nucleotides at the 5′ end called the poly-A tail ...
Deciphering the molecular phylogenetics of the Asian honey bee
Deciphering the molecular phylogenetics of the Asian honey bee

Comparative genomics exercises - Genome curation on emerging
Comparative genomics exercises - Genome curation on emerging

... Choose ‘Hidden’ from the drop-down box for A. oryzae (pink), A. nidulans (brown) and A. terreus (red). Choose ‘1 (reference)’ for A. fumigatus (purple). Choose ‘2’ for N. fischeri (green) and ‘3’ for A. clavatus (yellow). Click ‘redraw’. * To expand the range on either side of the reference cluster, ...
Ch12_lecture - Dr. Brahmbhatt`s Class Handouts
Ch12_lecture - Dr. Brahmbhatt`s Class Handouts

... 1. The desired gene is cloned. 2. First, one must obtain the gene; then, it must be inserted into a plasmid so that huge numbers of copies can be made. 3. Restriction enzymes cut the DNA at specific nucleotide sequences. 4. Genes are inserted into plasmids through the action of restriction enzymes i ...
Exercise II - GEP Community Server
Exercise II - GEP Community Server

... 13. Compare the Augustus prediction and the BLASTN evidence. You will find that they share the same exon-intron structure, but differ in the overall lengths: the gene model starts and ends further down-stream than the BLASTN evidence. 14. Use Exon Detail Editor to adjust the lengths of the flanking ...
Eukaryote-to-eukaryote gene transfer events revealed by the
Eukaryote-to-eukaryote gene transfer events revealed by the

... genes present in S288c were not found in the EC1118 genome (Table S4). Most of these genes are repeated and located in subtelomeric regions, which have not been accurately assembled, making it difficult to estimate copy number precisely. However, several of these genes (e.g., HXT16, PAU21, and SOR1) ...
Genome Mapping in the Horse
Genome Mapping in the Horse

... mapping have been classified based on the level of conservation. Type I markers are coding markers conserved across different species and typically having low polymorphism (e.g. CATS and SSCPs). By contrast, Type II markers, exemplified by microsatellites, are highly polymorphic markers, invaluable ...
PPT - Bioinfomed
PPT - Bioinfomed

... Clinical-genetic databases Genomics of pathogenic micro-organisms ...
The molecular basis of cytoplasmic male sterility and
The molecular basis of cytoplasmic male sterility and

... cytoplasm is derived from an interspecific hybridization of H. petiolaris and H. annuus. The 5-kb insertion created a 522-bp open reading frame (ORF) downstream of the atpA gene12 (Fig. 2). This ORF encodes a 16-kDa protein that accumulates in both sterile and restored PET1 seedlings13. Accumulation ...
Word - NIEHS SNPs Program - University of Washington
Word - NIEHS SNPs Program - University of Washington

... 6. Notice the SNP sites across the top of the image are listed by RS_ID. What is the RS_ID for the first nonsynonymous SNP from the left? 7. Close the VG2 image and Select Display Type windows and return to the Genome Variation Server Page. Now, change the allele frequency cutoff to 5. How many SNPs ...
Report The Derived FOXP2 Variant of Modern Humans Was Shared
Report The Derived FOXP2 Variant of Modern Humans Was Shared

... evolutionary changes in FOXP2, a gene that has been implicated in the development of speech and language. We furthermore find that in Neandertals, these changes lie on the common modern human haplotype, which previously was shown to have been subject to a selective sweep. These results suggest that ...
From gene to protein in higher plant mitochondria
From gene to protein in higher plant mitochondria

... chloroplastic enzyme having a sequence homologous to RNA polymerases found in bacteria) and two nuclearencoded bacteriophage-type RNA polymerases (NEP) (i.e. enzymes having their sequence homologous to bacteriophage RNA polymerases). In contrast, transcription in higher plant mitochondria seems to b ...
Semiconservative Replication in the Quasispecies Model
Semiconservative Replication in the Quasispecies Model

... {σ, σ̄} separates into the single-stranded sequences σ and σ̄. As strand separation occurs, daughter strand synthesis is catalyzed via enzymes known as DNA replicases. However, due to errors in the base pairing process, σ is not necessarily paired with σ̄. Rather, once cell division is finished, the ...
slides - ARUP.utah.edu - The University of Utah
slides - ARUP.utah.edu - The University of Utah

... „ May uncover copy number changes of unclear clinical significance „ Will not detect copy number changes in regions of the genome that are not on the array platform (chip) ...
View PDF - SciTechnol
View PDF - SciTechnol

... pairs; amplification efficiency; Tm similarity of all primers; and an optimal difference between amplified fragment lengths to permit resolution of products on a 2% agarose gel. For optimization of PCR conditions, we first optimized a pair of primers for standard PCR and then determined the approxim ...
14 Phylogenomic Approach to the Evolutionary Dynamics of Gene
14 Phylogenomic Approach to the Evolutionary Dynamics of Gene

... diverge to produce novel functions in a process known as neofunctionalization (Zhang, 2003). For example, some duplicated members of the RNaseA gene superfamily in primates evolved a novel antibacterial function that was not present in the common ancestral gene or its descendants (Zhang et al., 1998 ...
Gene duplications in prokaryotes can be
Gene duplications in prokaryotes can be

... Biclustering as it is implemented e.g. in the Biclustering Analysis Toolbox (BicAT) [16] clusters a data set in two dimensions simultaneously. This makes it possible to automatically identify clusters of organisms based on similarity within different subsets (clusters) of features, giving both an ov ...
DON`T COPY UNDERLINED TEXT Mrs. Aguirre`s Webpage
DON`T COPY UNDERLINED TEXT Mrs. Aguirre`s Webpage

... From the cross above, how many have: ONE HORN ______ out of 8 TWO HORNS _______ out of 8 Compare this number to your simulation (where you flipped the sticks). Does the punnett square predictions match the results of your crosses? A. They are exactly the same B. They are close to he same C. They are ...
Some Natural Language Processing (NLP) Tasks
Some Natural Language Processing (NLP) Tasks

... • stochastic context free grammars (SCFGs) represent variable-length sequences with long-range dependencies ...
Bacterial strain typing in the genomic era
Bacterial strain typing in the genomic era

... 2005). An automated apparatus for ribotyping is commercially available, with the RiboPrinterTM Microbial Characterization System (Qualicon, Wilmington, DE). This system provides highly reproducible and standardized ribotyping data. Ribobank is a ribotyping database comprising numerous bacterial ribo ...
< 1 ... 58 59 60 61 62 63 64 65 66 ... 445 >

Genome editing

Genome editing, or genome editing with engineered nucleases (GEEN) is a type of genetic engineering in which DNA is inserted, replaced, or removed from a genome using artificially engineered nucleases, or ""molecular scissors."" The nucleases create specific double-stranded break (DSBs) at desired locations in the genome, and harness the cell’s endogenous mechanisms to repair the induced break by natural processes of homologous recombination (HR) and nonhomologous end-joining (NHEJ). There are currently four families of engineered nucleases being used: Zinc finger nucleases (ZFNs), Transcription Activator-Like Effector Nucleases (TALENs), the CRISPR/Cas system, and engineered meganuclease re-engineered homing endonucleases.It is commonly practiced in genetic analysis that in order to understand the function of a gene or a protein function one interferes with it in a sequence-specific way and monitors its effects on the organism. However, in some organisms it is difficult or impossible to perform site-specific mutagenesis, and therefore more indirect methods have to be used, such as silencing the gene of interest by short RNA interference (siRNA) . Yet gene disruption by siRNA can be variable and incomplete. Genome editing with nucleases such as ZFN is different from siRNA in that the engineered nuclease is able to modify DNA-binding specificity and therefore can in principle cut any targeted position in the genome, and introduce modification of the endogenous sequences for genes that are impossible to specifically target by conventional RNAi. Furthermore, the specificity of ZFNs and TALENs are enhanced as two ZFNs are required in the recognition of their portion of the target and subsequently direct to the neighboring sequences.It was chosen by Nature Methods as the 2011 Method of the Year.
  • studyres.com © 2025
  • DMCA
  • Privacy
  • Terms
  • Report