Chapter 1 - Institut Montefiore
... same chromosome (see later) may not be independent. If not, they are said to be linked ...
... same chromosome (see later) may not be independent. If not, they are said to be linked ...
Diversity of DNA methyltransferases that recognize asymmetric
... and structural studies are required to confirm the relevance of this dimerization. The extent of subunit contacts observed in the crystal structure of M.MboIIA suggested that the two molecules in the asymmetric unit represent a biologically relevant dimer. In M.AhdI, two copies of the AhdIS subunit ...
... and structural studies are required to confirm the relevance of this dimerization. The extent of subunit contacts observed in the crystal structure of M.MboIIA suggested that the two molecules in the asymmetric unit represent a biologically relevant dimer. In M.AhdI, two copies of the AhdIS subunit ...
Specific oligonucleotide primers for detection of endoglucanase
... endoglucanase gene of B. subtilis, G. stearothermophilus and P. campinasensis. Two PCR primers, EN1F and EN1R, were chosen that were predicted to specifically amplify a 1,311 bpDNA fragment of the B. Subtilis, G. stearothermophilus and P. campinasensis. The Genbank database (NCBI) search for complim ...
... endoglucanase gene of B. subtilis, G. stearothermophilus and P. campinasensis. Two PCR primers, EN1F and EN1R, were chosen that were predicted to specifically amplify a 1,311 bpDNA fragment of the B. Subtilis, G. stearothermophilus and P. campinasensis. The Genbank database (NCBI) search for complim ...
C. elegans DAF-2 as a Model for Human Insulin Receptoropathies
... IIS pathway. We focused this study on the sole C. elegans insulinlike receptor, DAF-2, for several reasons: 1) 40 new missense alleles were present in the MMP collection allowing us to further assess DAF-2 structure-function; 2) reduction of DAF-2 activity results in an easily scored dauer phenotype ...
... IIS pathway. We focused this study on the sole C. elegans insulinlike receptor, DAF-2, for several reasons: 1) 40 new missense alleles were present in the MMP collection allowing us to further assess DAF-2 structure-function; 2) reduction of DAF-2 activity results in an easily scored dauer phenotype ...
A Novel CpG Island Set Identifies Tissue-Specific
... resulting library was sequenced to define a novel human blood CGI set that includes many that are not detected by current algorithms. Approximately half of CGIs were associated with annotated gene transcription start sites, the remainder being intra- or intergenic. Using an array representing over 1 ...
... resulting library was sequenced to define a novel human blood CGI set that includes many that are not detected by current algorithms. Approximately half of CGIs were associated with annotated gene transcription start sites, the remainder being intra- or intergenic. Using an array representing over 1 ...
Engineered Cpf1 Enzymes with Altered PAM Specificities
... oligonucleotides and HiScribe T7 in vitro Transcription Kit (NEB) following the manufacturer’s recommended protocol. For the PAM library, a degenerate 8 bp sequence preceding a 33 bp target site1 was cloned into the MCS in pUC19, and the library was digested with AatII and LguI and gel extracted pri ...
... oligonucleotides and HiScribe T7 in vitro Transcription Kit (NEB) following the manufacturer’s recommended protocol. For the PAM library, a degenerate 8 bp sequence preceding a 33 bp target site1 was cloned into the MCS in pUC19, and the library was digested with AatII and LguI and gel extracted pri ...
ANALYSIS OF MULTIPLE RESTRICTION FRAGMENT LENGTH
... probes and containing an insert of 14 .5 kb, termed XGSB 16.1, was restriction mapped (Fig. 1 B) . The Pvu II-Bam HI fragment, GB2PE, which contained neither human repeats nor CRI-1- and CRI-4-like sequences, hybridized to the 14.5-kb Bam HI fragment characteristic ofan S allele and to nonallelic fr ...
... probes and containing an insert of 14 .5 kb, termed XGSB 16.1, was restriction mapped (Fig. 1 B) . The Pvu II-Bam HI fragment, GB2PE, which contained neither human repeats nor CRI-1- and CRI-4-like sequences, hybridized to the 14.5-kb Bam HI fragment characteristic ofan S allele and to nonallelic fr ...
Bis2A 16.2 Errors in Meiosis
... one copy of an autosome invariably fail to develop to birth because they have only one copy of essential genes. Most autosomal trisomies also fail to develop to birth; however, duplications of some of the smaller chromosomes (13, 15, 18, 21, or 22) can result in ospring that survive for several wee ...
... one copy of an autosome invariably fail to develop to birth because they have only one copy of essential genes. Most autosomal trisomies also fail to develop to birth; however, duplications of some of the smaller chromosomes (13, 15, 18, 21, or 22) can result in ospring that survive for several wee ...
Gene density and transcription influence the localization of
... null hypothesis that the location of a distal 11p15.5 probe (e.g., cI-11p15-25) was the same as cq26 in 11p15.3, was rejected using a 2 sample t-test, P 0.000, but cI-11p15-25 has the same location as an adjacent probe cI-11p15-46, P 0.43. Volpi et al. (2000) similarly observed a high proportion ...
... null hypothesis that the location of a distal 11p15.5 probe (e.g., cI-11p15-25) was the same as cq26 in 11p15.3, was rejected using a 2 sample t-test, P 0.000, but cI-11p15-25 has the same location as an adjacent probe cI-11p15-46, P 0.43. Volpi et al. (2000) similarly observed a high proportion ...
Supplemental Material
... also posted at the AAA site, were also used in this analysis. The FlyBase inferred cytological map locations were assigned to all of the orthologs called in the four species. These associations were then ordered and sorted according to their scaffold assignments and molecular coordinates for each sp ...
... also posted at the AAA site, were also used in this analysis. The FlyBase inferred cytological map locations were assigned to all of the orthologs called in the four species. These associations were then ordered and sorted according to their scaffold assignments and molecular coordinates for each sp ...
wsp Gene Sequences from the Wolbachia of Filarial Nematodes
... coded by multicopy genes [13, 14]. For example, Ehrlichia chaffensis has at least six copies (tandemly arranged with intergenic spacers) of the gene coding for the major outer membrane proteins [13]. The proteins coded by these genes show up to approximately 20% amino acid difference. Trees based on ...
... coded by multicopy genes [13, 14]. For example, Ehrlichia chaffensis has at least six copies (tandemly arranged with intergenic spacers) of the gene coding for the major outer membrane proteins [13]. The proteins coded by these genes show up to approximately 20% amino acid difference. Trees based on ...
A Genetic Linkage Map of Mouse Chromosome 10
... et al. 1988). Third, molecular genetic linkage maps have been used for comparative mapping between mouse and human (or other) genomes [reviewed by NADEAU(1989) and SEARLE et al. (1 989)]. Comparative mapping can ultimately lead to the identification of mouse models of human diseases (BRUETON et a l ...
... et al. 1988). Third, molecular genetic linkage maps have been used for comparative mapping between mouse and human (or other) genomes [reviewed by NADEAU(1989) and SEARLE et al. (1 989)]. Comparative mapping can ultimately lead to the identification of mouse models of human diseases (BRUETON et a l ...
Gene Nomenclature System for Rice
... the RAP annotation database. Links to systematic_locus_IDs in other annotation databases (i.e., TIGR, BGI, etc.) will also be made where ever possible. Thus, at the time of registration, when a systemic locus identifier is provided to CGSNL, the version of the assembly and annotation must also be de ...
... the RAP annotation database. Links to systematic_locus_IDs in other annotation databases (i.e., TIGR, BGI, etc.) will also be made where ever possible. Thus, at the time of registration, when a systemic locus identifier is provided to CGSNL, the version of the assembly and annotation must also be de ...
Mapping the genes that made maize
... effects on the differences between the maize and teosinte ears. They suggested that each of the four chromosomal segments contained a group of morphological genes rather than a single gene, although they presented no evidence to support this view. Mangelsdorf5 reported that these four segments proba ...
... effects on the differences between the maize and teosinte ears. They suggested that each of the four chromosomal segments contained a group of morphological genes rather than a single gene, although they presented no evidence to support this view. Mangelsdorf5 reported that these four segments proba ...
SpliceCenter_DataBuild
... database build process. Dr. Kahn and Zeeberg developed a splice variant database, EVDB [5], which was based on NCBI Evidence Viewer [8] data. EVDB demonstrated the merits of constructing a repository of distinct splice variants that included each variant’s exon structure in chromosomal coordinates. ...
... database build process. Dr. Kahn and Zeeberg developed a splice variant database, EVDB [5], which was based on NCBI Evidence Viewer [8] data. EVDB demonstrated the merits of constructing a repository of distinct splice variants that included each variant’s exon structure in chromosomal coordinates. ...
functional analysis of chromatin assembly genes in tetrahymena
... The basic structural unit of chromatin is the nucleosome composed of ~147 base pairs of DNA wrapped around an octamer of histone proteins. Post-translational modifications such as histone acetylation or the substitution of histone variants in place of core histones have been implicated in various ch ...
... The basic structural unit of chromatin is the nucleosome composed of ~147 base pairs of DNA wrapped around an octamer of histone proteins. Post-translational modifications such as histone acetylation or the substitution of histone variants in place of core histones have been implicated in various ch ...
Modeling Trabecular Microstructure Evolution via Genetic Algorithm by Samuel W. L. Shames
... the same set of points under the same stress state at three stages of evolution. The figure shows how drastically the microstructure of the system can change in only a small number of generations and how the general tendency is for the number of struts with a large strain energy—bright red struts—to ...
... the same set of points under the same stress state at three stages of evolution. The figure shows how drastically the microstructure of the system can change in only a small number of generations and how the general tendency is for the number of struts with a large strain energy—bright red struts—to ...
Supplemental Tables
... also posted at the AAA site, were also used in this analysis. The FlyBase inferred cytological map locations were assigned to all of the orthologs called in the four species. These associations were then ordered and sorted according to their scaffold assignments and molecular coordinates for each sp ...
... also posted at the AAA site, were also used in this analysis. The FlyBase inferred cytological map locations were assigned to all of the orthologs called in the four species. These associations were then ordered and sorted according to their scaffold assignments and molecular coordinates for each sp ...
Genetic Control of X Chromosome Inactivation in Mice: Definition of
... B6CASTF2 Xce homozygous females using a panel of microsatellite markers with 20 cM average spacing based on one developed previously (Iakoubova et al. 2000). We identified a single major QTL (Figure 1) in this cross. As this locus was X linked, it seemed likely that this association was due to Xce. ...
... B6CASTF2 Xce homozygous females using a panel of microsatellite markers with 20 cM average spacing based on one developed previously (Iakoubova et al. 2000). We identified a single major QTL (Figure 1) in this cross. As this locus was X linked, it seemed likely that this association was due to Xce. ...
The murine homologue of HIRA, a DiGeorge
... acids 510 and 607 or 608. Our Hira sequence is 99% identical to the recently reported mouse Hira sequence (35) and 100% identical to the mouse Tuple1 cDNA clone MF2 (26). In addition to homology with yeast Hir1p and Hir2p, BlastP and BEAUTY database searches with our mouse Hira sequence reveal high ...
... acids 510 and 607 or 608. Our Hira sequence is 99% identical to the recently reported mouse Hira sequence (35) and 100% identical to the mouse Tuple1 cDNA clone MF2 (26). In addition to homology with yeast Hir1p and Hir2p, BlastP and BEAUTY database searches with our mouse Hira sequence reveal high ...
15.13 Spm elements influence gene expression
... originally classified as transposons, but now considered to be episomes. The mechanism of movement resembles that of phage lambda. Nonreplicative transposition describes the movement of a transposon that leaves a donor site (usually generating a double-strand break) and moves to a new site. Replicat ...
... originally classified as transposons, but now considered to be episomes. The mechanism of movement resembles that of phage lambda. Nonreplicative transposition describes the movement of a transposon that leaves a donor site (usually generating a double-strand break) and moves to a new site. Replicat ...
Figure 15.6 Nonreplicative transposition allows a transposon to
... originally classified as transposons, but now considered to be episomes. The mechanism of movement resembles that of phage lambda. Nonreplicative transposition describes the movement of a transposon that leaves a donor site (usually generating a double-strand break) and moves to a new site. Replicat ...
... originally classified as transposons, but now considered to be episomes. The mechanism of movement resembles that of phage lambda. Nonreplicative transposition describes the movement of a transposon that leaves a donor site (usually generating a double-strand break) and moves to a new site. Replicat ...
Biophysics 101 Genomics and Computational Biology
... RNA 1: Microarrays, library sequencing & quantitation concepts RNA 2: Clustering by gene or condition & other regulon data sources RNA 3: Nucleic acid motifs; the nature of biological "proofs". Protein 1: 3D structural genomics, homology, dynamics, function & drug design Protein 2: Mass spectrometry ...
... RNA 1: Microarrays, library sequencing & quantitation concepts RNA 2: Clustering by gene or condition & other regulon data sources RNA 3: Nucleic acid motifs; the nature of biological "proofs". Protein 1: 3D structural genomics, homology, dynamics, function & drug design Protein 2: Mass spectrometry ...
Human genome
The human genome is the complete set of nucleic acid sequence for humans (Homo sapiens), encoded as DNA within the 23 chromosome pairs in cell nuclei and in a small DNA molecule found within individual mitochondria. Human genomes include both protein-coding DNA genes and noncoding DNA. Haploid human genomes, which are contained in germ cells (the egg and sperm gamete cells created in the meiosis phase of sexual reproduction before fertilization creates a zygote) consist of three billion DNA base pairs, while diploid genomes (found in somatic cells) have twice the DNA content. While there are significant differences among the genomes of human individuals (on the order of 0.1%), these are considerably smaller than the differences between humans and their closest living relatives, the chimpanzees (approximately 4%) and bonobos. Humans share 50% of their DNA with bananas.The Human Genome Project produced the first complete sequences of individual human genomes, with the first draft sequence and initial analysis being published on February 12, 2001. The human genome was the first of all vertebrates to be completely sequenced. As of 2012, thousands of human genomes have been completely sequenced, and many more have been mapped at lower levels of resolution. The resulting data are used worldwide in biomedical science, anthropology, forensics and other branches of science. There is a widely held expectation that genomic studies will lead to advances in the diagnosis and treatment of diseases, and to new insights in many fields of biology, including human evolution.Although the sequence of the human genome has been (almost) completely determined by DNA sequencing, it is not yet fully understood. Most (though probably not all) genes have been identified by a combination of high throughput experimental and bioinformatics approaches, yet much work still needs to be done to further elucidate the biological functions of their protein and RNA products. Recent results suggest that most of the vast quantities of noncoding DNA within the genome have associated biochemical activities, including regulation of gene expression, organization of chromosome architecture, and signals controlling epigenetic inheritance.There are an estimated 20,000-25,000 human protein-coding genes. The estimate of the number of human genes has been repeatedly revised down from initial predictions of 100,000 or more as genome sequence quality and gene finding methods have improved, and could continue to drop further. Protein-coding sequences account for only a very small fraction of the genome (approximately 1.5%), and the rest is associated with non-coding RNA molecules, regulatory DNA sequences, LINEs, SINEs, introns, and sequences for which as yet no function has been elucidated.