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Reduced amino acid alphabets exhibit an improved sensitivity and
Reduced amino acid alphabets exhibit an improved sensitivity and

... of structures and their associated sequences now available in the Protein Data Bank (PDB), it is clear that the same protein fold may be generated by different amino acid sequences; in effect, the structure of proteins is more conserved than their sequences (Chothia and Lesk, 1986). In some cases, t ...
The Calcium-Binding Activity of a Vacuole
The Calcium-Binding Activity of a Vacuole

... largely depleted in evacuolated protoplasts and, conversely, is enriched in isolated vacuoles from tobacco cells (Fig. 3B). However, based on all these data, we cannot conclude that VCaB45 localization is restricted to the vacuole. VCaB45 could be quantitatively removed from the low-density membrane ...
characteristics and stabilization of dnaase
characteristics and stabilization of dnaase

... cell-free extracts.lP3 One object of the present investigation was to study this phenomenon further. A major difficulty in the study of cell-free protein synthesis in h’. coli systems has been the necessity for preparing fresh enzyme extracts for each experiment. Techniques have not been available f ...
The Crystal Structure of Arabidopsis thaliana Allene Oxide Cyclase
The Crystal Structure of Arabidopsis thaliana Allene Oxide Cyclase

... of the cloned AOCs revealed the presence of transit peptides for plastid targeting, and localization in chloroplasts was confirmed immunohistochemically (Ziegler et al., 2000; Stenzel et al., 2003a, 2003b) and by transient expression of AOC1-4/green fluorescent protein fusions (F. Schaller and P. Ze ...
the enzymatic synthesis of protein. i.
the enzymatic synthesis of protein. i.

... important observation that neither enzyme was capable of producing plastein from any type of digest of gelatin; nor from any tryptic digest except of casein, and here only by pepsin. There is indicated in their work the possibility of the influence of dilution, of temperature, and of acidity on the ...
A General Method for the Determination of the Carboxyl
A General Method for the Determination of the Carboxyl

... The general principle behind the isolation procedure is similar to that described by Furka et al. (6). When a protein is digested with a proteolytic enzyme, each bond cleaved generates a new amino and a new carboxyl group. If the digestion is performed on a protein in which all carboxyl groups have ...
Gelatinization of Starch
Gelatinization of Starch

...  Starch acetates or phosphates are common modifications, altering the helical arrangements, and slow or inhibit retrogradation.  All stabilized starches must also be labeled as “modified starch” on an ingredient list. ...
BRNO UNIVERSITY OF TECHNOLOGY FACULTY OF
BRNO UNIVERSITY OF TECHNOLOGY FACULTY OF

... modification in plants because glycoproteins play a key role in various biological functions. Since glycoproteins are often present in a small amount, their enrichment from a complex mixture is required for their analysis. Lectin concanavalin A affinity chromatography was used for barley glycoprotei ...
Document
Document

FREE Sample Here
FREE Sample Here

... Full file at http://testbankeasy.eu/Test-bank-for-Molecular-Cell-Biology,-7thEdition---Harvey-Lodish 30. What is the effect of an enzyme on the end equilibrium concentration of reactants and products? Ans: An enzyme has no effect on the end equilibrium concentration of reactants and products. 31. T ...
Redalyc.MOLECULAR CHARACTERIZATION OF CRUDE SEED
Redalyc.MOLECULAR CHARACTERIZATION OF CRUDE SEED

... Santos et al. (2005) and Madrona et al. (2012) reported a molecular weight of 30kDa. These results confirm the presence of isoforms of the same protein. The calculation of the molecular weights by the Quantity One 4.6.5 program is shown in Table  II. For the extract of M. oleifera with distilled wat ...
Hidden Markov Model for protein secondary structure
Hidden Markov Model for protein secondary structure

... 2.25 Å and less than 25% sequence identity. Secondary structure definition is given by an assignment method developed in our laboratory (manuscript in preparation) or by STRIDE method [Frishman and Argos, 1995]. 489743 residues have a defined secondary structure in our data set. 2024 sequences, ran ...
ppt - Avraham Samson`s Lab
ppt - Avraham Samson`s Lab

Structural bases of GM1 gangliosidosis and Morquio B
Structural bases of GM1 gangliosidosis and Morquio B

... in the catalytic domain, and is located near the active site (Figure 3). The loop region contains three active site residues (I126, A128 and E129), and interacts with the two loops containing the catalytic residues, namely E188 and E268. The ASA value of G123 is 0 Å2 (Table 1), which means that G12 ...
Cholesterol and Lipid T Port
Cholesterol and Lipid T Port

... Greasy Spoon Digestion and Transport   Chylomicrons (98-99% lipid 1-2% protein) - transport of dietary lipids into circulation - mostly TAGs some phospholipid and cholerol esters - Initially synthesized in intestine, 1/2 in rats min, in humans 30 mins - transport FA from lymphatic system to blood s ...
amino acids
amino acids

... How to calculate the number of amino acids in a protein • We can calculate the approximate number of amino acid residues in a simple protein containing no other chemical constituents by dividing its molecular weight by 110. • Although the average molecular weight of the 20 common amino acids is abo ...
Nitrogen Balance and Protein Requirements: Definition and
Nitrogen Balance and Protein Requirements: Definition and

... The diurnal cycle of feeding and fasting is accompanied by concurrent changes in protein turnover. Protein feeding is necessary to replenish the body protein stores that would be wasted during fasting [21–24]. Because of this, nitrogen retention calculated on a daily basis is lower than that derived ...
34750 - Radboud Repository
34750 - Radboud Repository

... from intermediate metabolism, such as synthesis of heme groups [1], steroids [2], amino acids, and iron-sulphur (Fe-S) clusters [3]. Phylogenetic analyses of mitochondrial genes indicate that all mitochondria derive from a single alphaproteobacterial ancestor, the so-called proto-mitochondrion [4]. ...
N - IBIVU
N - IBIVU

... bidirectional recurrent neural networks – One basic sliding window is used, with two more windows that slight in from opposite sites at each basic window position. This way allpossible long-range interactions are checked. ...
An Efficient Protocol for Identifying Separation-of-Function
An Efficient Protocol for Identifying Separation-of-Function

... referred to as separation-of-function (sof2) alleles, which surgically eliminate a single biochemical function while leaving other activities (and presumably the structural integrity of the protein) intact. The advantages of this particular class of mutations in elucidating biological pathways, part ...
Lecture Note 6
Lecture Note 6

... crystal stuture of the biological macromolecules. It is co-ordinated by the consortium located in Europe, Japan and USA. As of August 2013, the database contains 93043 structures which includes protein, nucleic acids, and protein-nucleic acid or proteinsmall molecule complexes (http://www.rcsb.org/p ...
Structure and function of the chloroplast signal recognition particle
Structure and function of the chloroplast signal recognition particle

... Jonas-Straube et al. 2001). Later, a third chromodomain located near the N-terminus of the mature protein was predicted (Eichacker and Henry 2001). It will be an interesting quest for the future to get detailed information about the role of the different domains in the formation of the cpSRP and the ...
Protein hydrolysates in sports nutrition
Protein hydrolysates in sports nutrition

... Collectively, these studies strongly suggest that ingestion of a fast-acting protein hydrolysate and/or amino acid supplement results a less efficient uptake by the splanchnic bed and therefore increases the magnitude of the acute increase in amino acids in the systemic circulation that are availab ...
Projection Structure of a Plant Vacuole Membrane Aquaporin by
Projection Structure of a Plant Vacuole Membrane Aquaporin by

... into two mirror-image groups according to patterns of diffraction intensity and unit cell angle, indicating good preservation of both sides of the 2D crystals. The a axis was found to be at an angle of 7  relative to the longitudinal axis of the tube. Data from 15 images with tilt angles of 0  to ...
lH NMR characterization of two crambin species
lH NMR characterization of two crambin species

... amino acid analyses were carried out. The results are shown in table 1. First, it can be noted that the HPLC separation, which is likely to be based on the Pro/Ser substitution at position 22 and not at the Leu/Ile substitution at 25, has yielded a more hydrophobic Pro-22 species in fraction II as e ...
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Interactome



In molecular biology, an interactome is the whole set of molecular interactions in a particular cell. The term specifically refers to physical interactions among molecules (such as those among proteins, also known as protein-protein interactions) but can also describe sets of indirect interactions among genes (genetic interactions). Mathematically, interactomes are generally displayed as graphs.The word ""interactome"" was originally coined in 1999 by a group of French scientists headed by Bernard Jacq. Though interactomes may be described as biological networks, they should not be confused with other networks such as neural networks or food webs.
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