The mapping of linear B-cell epitope regions in desmoglein 1 and 3
... proteins can be an alternative way to identify epitope regions because of the sufficient similarity to the native antigen which allows the binding of antibodies. This is a rapid, practical, and cost-effective method for linear epitope region identification [20, 21]. B-cell epitope mapping using a se ...
... proteins can be an alternative way to identify epitope regions because of the sufficient similarity to the native antigen which allows the binding of antibodies. This is a rapid, practical, and cost-effective method for linear epitope region identification [20, 21]. B-cell epitope mapping using a se ...
Type and timing of protein feeding to optimize anabolism
... suggest that a decrease of whole-body protein degradation in the post-prandial state requires a prolonged increase (even moderate) of blood free amino acid levels. This was the case when subjects were fed casein or frequent small meals [10]. The main target in this situation appears to be in the spl ...
... suggest that a decrease of whole-body protein degradation in the post-prandial state requires a prolonged increase (even moderate) of blood free amino acid levels. This was the case when subjects were fed casein or frequent small meals [10]. The main target in this situation appears to be in the spl ...
STAAR Biology Assessment Activities Sample
... The purpose of this activity is to reinforce students’ understanding that systems in animals constantly interact with one another to keep the animals functioning properly. Students will use an interactive manipulative activity to demonstrate an understanding of the importance of interactions between ...
... The purpose of this activity is to reinforce students’ understanding that systems in animals constantly interact with one another to keep the animals functioning properly. Students will use an interactive manipulative activity to demonstrate an understanding of the importance of interactions between ...
Folie 1 - FLI
... Structural genomics consists in the determination of the three dimensional structure of all proteins of a given organism, by experimental methods such as X-ray crystallography, NMR spectroscopy or computational approaches such as homology modelling. As opposed to traditional structural biology, the ...
... Structural genomics consists in the determination of the three dimensional structure of all proteins of a given organism, by experimental methods such as X-ray crystallography, NMR spectroscopy or computational approaches such as homology modelling. As opposed to traditional structural biology, the ...
HiFliQ Ni-NTA FPLC Columns - User Guide
... cell pellet under native conditions using Protein Ark HiFliQ Ni-NTA FPLC Columns. Reagent amounts given apply to IPTG-induced bacterial culture of a well-expressed protein (approximately 10-50 mg/l). Cells are lysed with lysozyme because it is an inexpensive and efficient method for cells that have ...
... cell pellet under native conditions using Protein Ark HiFliQ Ni-NTA FPLC Columns. Reagent amounts given apply to IPTG-induced bacterial culture of a well-expressed protein (approximately 10-50 mg/l). Cells are lysed with lysozyme because it is an inexpensive and efficient method for cells that have ...
video slide
... Nucleotide Polymers – Are made up of nucleotides linked by the–OH group on the 3´ carbon of one nucleotide and the phosphate on the 5´ carbon on the next ...
... Nucleotide Polymers – Are made up of nucleotides linked by the–OH group on the 3´ carbon of one nucleotide and the phosphate on the 5´ carbon on the next ...
PDF only - at www.arxiv.org.
... high-throughput 3D structure-determination methods (experimental or predicted) of proteins have brought about the accumulation of protein structures which completely lack functional information (Bentley et al., 2004; Murphy et al, 2004; Baxevanis, 2003; Miller et al,, 2003). For instance, the Protei ...
... high-throughput 3D structure-determination methods (experimental or predicted) of proteins have brought about the accumulation of protein structures which completely lack functional information (Bentley et al., 2004; Murphy et al, 2004; Baxevanis, 2003; Miller et al,, 2003). For instance, the Protei ...
prediction
... training the method on the remaining training database containing the N1 remaining sequences (one-at-a-time jackknife testing). A complete jackknife test would involve N such predictions. If N is large enough, meaningful statistics can be derived from the observed performance. For example, the mean ...
... training the method on the remaining training database containing the N1 remaining sequences (one-at-a-time jackknife testing). A complete jackknife test would involve N such predictions. If N is large enough, meaningful statistics can be derived from the observed performance. For example, the mean ...
Functional Requirements of the Yellow Fever Virus Capsid Protein
... recently been reported and exhibits a fold that is very similar overall to that of DENC, with the exception of the relative orientation of ␣1 (10). Despite the low sequence identity of the flavivirus C proteins, certain features are well conserved in all flaviviruses (Fig. 1A). Firstly, the flavivir ...
... recently been reported and exhibits a fold that is very similar overall to that of DENC, with the exception of the relative orientation of ␣1 (10). Despite the low sequence identity of the flavivirus C proteins, certain features are well conserved in all flaviviruses (Fig. 1A). Firstly, the flavivir ...
Effective Scoring Function for Protein Sequence Design
... structures. The solvation energy of non-hydrogenbonded hydrophilic atoms was considered separately and expressed in a nonlinear way. As a result, our scoring function predicted native residues as the most favorable in 59% of the total positions in 28 proteins. We then tested the scoring function by ...
... structures. The solvation energy of non-hydrogenbonded hydrophilic atoms was considered separately and expressed in a nonlinear way. As a result, our scoring function predicted native residues as the most favorable in 59% of the total positions in 28 proteins. We then tested the scoring function by ...
Pavel Doležal
... organisms were then called “amitochondriates”. More recent studies of evolution and cell biology of “amitochondriate” protists, however, challenged their amitochondriate status, at least in the case of those organisms possessing double-membrane bound organelles such as hydrogen-producing hydrogenoso ...
... organisms were then called “amitochondriates”. More recent studies of evolution and cell biology of “amitochondriate” protists, however, challenged their amitochondriate status, at least in the case of those organisms possessing double-membrane bound organelles such as hydrogen-producing hydrogenoso ...
Conservation and relative importance of residues across protein
... fully buried in the interface, whereas the latter only contains atoms that retain partial accessibility (24, 25). The core possesses more hydrophobic residues and has a composition that is distinct from the rim or the rest of the protein surface. With the division into core and rim residues one can ...
... fully buried in the interface, whereas the latter only contains atoms that retain partial accessibility (24, 25). The core possesses more hydrophobic residues and has a composition that is distinct from the rim or the rest of the protein surface. With the division into core and rim residues one can ...
Development of Amino Acid as Parenteral Nutrition P N
... Parenteral Amino Acid in Clinical Setting Protein is the most abundant nitrogen-containing compound in the diet and in the human body. It is one of the complex bio-molecules present in cells and tissues. All amino acids (AAs) have a similar chemical structure-each contains an amino group (NH2), an a ...
... Parenteral Amino Acid in Clinical Setting Protein is the most abundant nitrogen-containing compound in the diet and in the human body. It is one of the complex bio-molecules present in cells and tissues. All amino acids (AAs) have a similar chemical structure-each contains an amino group (NH2), an a ...
Electrophoretic Properties of Native Proteins
... Proteins exhibit many different three-dimensional shapes and complex folding patterns which are determined by their amino acid sequence and post translational processing such as adding carbohydrate residues or prosthetic groups. The precise three-dimensional configuration of a protein is critical to ...
... Proteins exhibit many different three-dimensional shapes and complex folding patterns which are determined by their amino acid sequence and post translational processing such as adding carbohydrate residues or prosthetic groups. The precise three-dimensional configuration of a protein is critical to ...
Functomics!?
... or mutation of regions involved in activity or binding (to metals, nucleotides, etc), presence of paralogs, contradiction with the biological context (i.e. if a protein belongs to a pathway supposed to be absent in a particular organism), etc. Such "problematic" proteins will not be automatically an ...
... or mutation of regions involved in activity or binding (to metals, nucleotides, etc), presence of paralogs, contradiction with the biological context (i.e. if a protein belongs to a pathway supposed to be absent in a particular organism), etc. Such "problematic" proteins will not be automatically an ...
Protease Activity of a 90-kDa Protein Isolated from Scallop Shells
... chromatography (Figure 1A). Subsequently, the protease activity of each fraction was examined, and it was found in 2 fractions (fractions 9–13 and 16–18). The high molecular weight fractions (fraction 9–13) showing higher enzyme activity were pooled and subjected to DEAE-cellulose column chromatogra ...
... chromatography (Figure 1A). Subsequently, the protease activity of each fraction was examined, and it was found in 2 fractions (fractions 9–13 and 16–18). The high molecular weight fractions (fraction 9–13) showing higher enzyme activity were pooled and subjected to DEAE-cellulose column chromatogra ...
Lecture 2
... adjacent strand through hydrogen bonds. Amino acids in anti-parallel strands on the other hand interact with only one amino acid on an adjacent strand. Protein secondary structures – Turns & Loops: Almost all proteins exhibit a compact, globular structure which is possible only if there are turns or ...
... adjacent strand through hydrogen bonds. Amino acids in anti-parallel strands on the other hand interact with only one amino acid on an adjacent strand. Protein secondary structures – Turns & Loops: Almost all proteins exhibit a compact, globular structure which is possible only if there are turns or ...
Journal of the Science of Food and Agriculture
... and they contain larger amounts than most of the common cereal grains.8,12 The protein content of amaranth is about 16%.7 The nutritional quality of amaranth proteins is also very high in comparison to cereals and some legumes.13 Amaranth has high contents of lysine, arginine, tryptophan and sulphur ...
... and they contain larger amounts than most of the common cereal grains.8,12 The protein content of amaranth is about 16%.7 The nutritional quality of amaranth proteins is also very high in comparison to cereals and some legumes.13 Amaranth has high contents of lysine, arginine, tryptophan and sulphur ...
Derivation and testing of pair potentials for protein folding. When is
... Here, ny is the number of residues and I, is the average number of contacts of residue type y . A number of conceptual problems are associated with the quasichemical approximation. First of all, it ignores chain connectivity. As pointed out by Jemigan and Bahar (1996), this neglect of chain connecti ...
... Here, ny is the number of residues and I, is the average number of contacts of residue type y . A number of conceptual problems are associated with the quasichemical approximation. First of all, it ignores chain connectivity. As pointed out by Jemigan and Bahar (1996), this neglect of chain connecti ...
1 - GET Test Bank
... Full file at http://gettestbank.eu/Test-Bank-for-Molecular-Cell-Biology,-7th-Edition-Harvey-Lodish 30. What is the effect of an enzyme on the end equilibrium concentration of reactants and products? Ans: An enzyme has no effect on the end equilibrium concentration of reactants and products. 31. The ...
... Full file at http://gettestbank.eu/Test-Bank-for-Molecular-Cell-Biology,-7th-Edition-Harvey-Lodish 30. What is the effect of an enzyme on the end equilibrium concentration of reactants and products? Ans: An enzyme has no effect on the end equilibrium concentration of reactants and products. 31. The ...
Interactome
In molecular biology, an interactome is the whole set of molecular interactions in a particular cell. The term specifically refers to physical interactions among molecules (such as those among proteins, also known as protein-protein interactions) but can also describe sets of indirect interactions among genes (genetic interactions). Mathematically, interactomes are generally displayed as graphs.The word ""interactome"" was originally coined in 1999 by a group of French scientists headed by Bernard Jacq. Though interactomes may be described as biological networks, they should not be confused with other networks such as neural networks or food webs.