PCNA Protein Expression during Spermatogenesis of the
... PCNA are derived from different mRNAs produced by alternative splicing processes. The two proteins were equally recognized by antibodies raised against different epitopes, and were identical in the N-terminal amino acid sequence. Anti-phosphoamino acid antibodies recognized the 36-kDa form of PCNA b ...
... PCNA are derived from different mRNAs produced by alternative splicing processes. The two proteins were equally recognized by antibodies raised against different epitopes, and were identical in the N-terminal amino acid sequence. Anti-phosphoamino acid antibodies recognized the 36-kDa form of PCNA b ...
The Anaerobic (Class III) Ribonucleotide Reductase from Lactococcus lactis
... Expression and Purification of NrdD—E. coli IG016 was grown microaerophilically in 1.6-liter batches at 37 °C in LB medium containing 2% glucose, 150 g/ml kanamycin, and 34 g/ml chloramphenicol with continuous flow-through of 4% CO2 and 96% N2. When the culture had reached mid-log phase (A550 ⫽ 0. ...
... Expression and Purification of NrdD—E. coli IG016 was grown microaerophilically in 1.6-liter batches at 37 °C in LB medium containing 2% glucose, 150 g/ml kanamycin, and 34 g/ml chloramphenicol with continuous flow-through of 4% CO2 and 96% N2. When the culture had reached mid-log phase (A550 ⫽ 0. ...
Protein proteinase inhibitors from avian egg whites
... secretion of ovoinhibitor in oviduct is controlled by estrogen and progesterone, and is also stimulated by insulin and dexamethasone. Ovoinhibitors from chicken, turkey, Japanese quail, penguin and ostrich have been isolated and studied in detail. The protein is immunologically related to the a2-pro ...
... secretion of ovoinhibitor in oviduct is controlled by estrogen and progesterone, and is also stimulated by insulin and dexamethasone. Ovoinhibitors from chicken, turkey, Japanese quail, penguin and ostrich have been isolated and studied in detail. The protein is immunologically related to the a2-pro ...
MPN+, a putative catalytic motif found in a subset of MPN domain
... amino group on the substrate [3,4]. Proteolysis takes place within the 20S core particle (CP) of the proteasome, while the 19S regulatory particle (RP) binds polyubiquitinated substrates, unfolds, and translocates them into the 20S CP for proteolysis. The discovery that the 19S regulatory particle o ...
... amino group on the substrate [3,4]. Proteolysis takes place within the 20S core particle (CP) of the proteasome, while the 19S regulatory particle (RP) binds polyubiquitinated substrates, unfolds, and translocates them into the 20S CP for proteolysis. The discovery that the 19S regulatory particle o ...
Understanding the acetylome: translating targeted
... duration, and recovery shaping skeletal muscle plasticity. Given the power of physical activity to regulate metabolic health, numerous research groups have focused on the molecular mechanisms that sense, interpret, and translate this contractile signal into postexercise adaptation. While our current ...
... duration, and recovery shaping skeletal muscle plasticity. Given the power of physical activity to regulate metabolic health, numerous research groups have focused on the molecular mechanisms that sense, interpret, and translate this contractile signal into postexercise adaptation. While our current ...
Abiotic stress in plants: Late Embryogenesis Abundant proteins Imen Amara
... application. Various stress signals and ABA share common elements in their signaling pathways, with common elements that cross talk with each other in order to maintain cellular homeostasis, (Cramer et al. 2011; Wasilewska et al. 2008). Drought, salt and cold stress produce extensive changes in the ...
... application. Various stress signals and ABA share common elements in their signaling pathways, with common elements that cross talk with each other in order to maintain cellular homeostasis, (Cramer et al. 2011; Wasilewska et al. 2008). Drought, salt and cold stress produce extensive changes in the ...
Enzymes of the mevalonate pathway of isoprenoid
... The mevalonate pathway accounts for conversion of acetyl-CoA to isopentenyl 5-diphosphate, the versatile precursor of polyisoprenoid metabolites and natural products. The pathway functions in most eukaryotes, archaea, and some eubacteria. Only recently has much of the functional and structural basis ...
... The mevalonate pathway accounts for conversion of acetyl-CoA to isopentenyl 5-diphosphate, the versatile precursor of polyisoprenoid metabolites and natural products. The pathway functions in most eukaryotes, archaea, and some eubacteria. Only recently has much of the functional and structural basis ...
Molecular insights into RNA and DNA helicase evolution from the
... eLife digest Living cells store their genetic material as DNA, which can be copied to make another molecule called RNA. DNA consists of two strands that are wound around each other in a double helix. RNA is made in a similar way to DNA, but it is usually present as a single strand that folds into a ...
... eLife digest Living cells store their genetic material as DNA, which can be copied to make another molecule called RNA. DNA consists of two strands that are wound around each other in a double helix. RNA is made in a similar way to DNA, but it is usually present as a single strand that folds into a ...
Multiple Sequence Motifs in the Rubisco Small
... the expression of atTOC159 is high in young and photosynthetic tissues whereas atTOC132 and atTOC120 are expressed uniformly in all plant tissues at low levels (Kubis et al., 2004). In addition, in nonphotosynthetic tissues, such as roots, the mRNA level of atTOC132 or atTOC120 is much higher than t ...
... the expression of atTOC159 is high in young and photosynthetic tissues whereas atTOC132 and atTOC120 are expressed uniformly in all plant tissues at low levels (Kubis et al., 2004). In addition, in nonphotosynthetic tissues, such as roots, the mRNA level of atTOC132 or atTOC120 is much higher than t ...
Isolation of a Strong Suppressor of Nonsense Mutations in Bacillus
... acid were inserted which made an inactive protein. To decide between these two possibilities, the proteins synthesized in ultraviolet-irradiated bacteria infected with mutant susB47 were analyzed by gel electrophoresis. Bacteria infected with mutant susN212, which grows on both suppressor strains, w ...
... acid were inserted which made an inactive protein. To decide between these two possibilities, the proteins synthesized in ultraviolet-irradiated bacteria infected with mutant susB47 were analyzed by gel electrophoresis. Bacteria infected with mutant susN212, which grows on both suppressor strains, w ...
The world of proteases Diversity and function
... Protease activity can be measured in many ways using different substrates and different reaction conditions There is not one correct way to measure protease activity An activity number is always dependent on the exact assay conditions Therefore, activity units/protease assays cannot be used to evalu ...
... Protease activity can be measured in many ways using different substrates and different reaction conditions There is not one correct way to measure protease activity An activity number is always dependent on the exact assay conditions Therefore, activity units/protease assays cannot be used to evalu ...
Development of Software Package for Determining Protein
... Asp102 of Chymotrypsin – hydrogen bond with His57 – increases pKa His57 can accepts proton from Ser195 – activates serine protease for cleavage of substrate pKa shift important for each chemical reaction in catalytic mechanism Necessary to donate and abstract protons from neighboring groups Without ...
... Asp102 of Chymotrypsin – hydrogen bond with His57 – increases pKa His57 can accepts proton from Ser195 – activates serine protease for cleavage of substrate pKa shift important for each chemical reaction in catalytic mechanism Necessary to donate and abstract protons from neighboring groups Without ...
The Structure of the PanD/PanZ Protein Complex
... backbone between residues Gly24 and Ser25, leading to the formation of a pyruvoyl cofactor from Ser25 (Figure 1B). The residues in PanD required for activation have been previously explored by mutagenesis. As expected, mutation of Ser25 to alanine leads to loss of activation (Schmitzberger et al., 2 ...
... backbone between residues Gly24 and Ser25, leading to the formation of a pyruvoyl cofactor from Ser25 (Figure 1B). The residues in PanD required for activation have been previously explored by mutagenesis. As expected, mutation of Ser25 to alanine leads to loss of activation (Schmitzberger et al., 2 ...
Titration curves of proteins
... In this chapter, I will give the theoretical background and describe a method how the titration curves of the proteins can be calculated. After a brief introduction about acid-base and redox equilibria, the electrostatic calculation of the protonation and oxidation probabilities by solving the Poiss ...
... In this chapter, I will give the theoretical background and describe a method how the titration curves of the proteins can be calculated. After a brief introduction about acid-base and redox equilibria, the electrostatic calculation of the protonation and oxidation probabilities by solving the Poiss ...
Strain in Protein Structures as Viewed Through Nonrotameric Side
... side chain forms the backbone. By using data from 19 well-refined crystallographic structures, Ponder and Richards6 assembled a library of rotamers for each amino acid type based on clustering techniques. They observed that most side chains adopt one of the rotameric states. Schrauber et al.1 used a ...
... side chain forms the backbone. By using data from 19 well-refined crystallographic structures, Ponder and Richards6 assembled a library of rotamers for each amino acid type based on clustering techniques. They observed that most side chains adopt one of the rotameric states. Schrauber et al.1 used a ...
PeptidePicker: a Tool for Determining Most Appropriate Peptides for
... Multiple Reaction Monitoring (MRM), also known as Selected Reaction Monitoring (SRM), is a powerful method for performing targeted proteomics. The approach is based on the generation of specific quantitative assays for each protein of interest and can be used to accurately quantitate large sets of p ...
... Multiple Reaction Monitoring (MRM), also known as Selected Reaction Monitoring (SRM), is a powerful method for performing targeted proteomics. The approach is based on the generation of specific quantitative assays for each protein of interest and can be used to accurately quantitate large sets of p ...
Modulation of functional properties of bifunctional S- Adenosylmethionine decarboxylase/Ornithine decarboxylase of
... Trishydroxy (methyl-amino) methane / Hydrochloric acid ...
... Trishydroxy (methyl-amino) methane / Hydrochloric acid ...
World Index of BioMolecular Visualization Resources
... Cross-indexing terms: deoxyribonucleic acid, DNA, major groove, minor groove. This Chime resource contains animations to illustrate the structure of DNA. The animations are initiated by clicking buttons on the right pannel. They show a base pair in the context of a larger helix and highlight the bas ...
... Cross-indexing terms: deoxyribonucleic acid, DNA, major groove, minor groove. This Chime resource contains animations to illustrate the structure of DNA. The animations are initiated by clicking buttons on the right pannel. They show a base pair in the context of a larger helix and highlight the bas ...
TETHERING: Fragment-Based Drug Discovery
... several groups (7, 44, 53) have used crystallography to discover and develop fragments. Crystallography can also be combined with NMR to yield a hybrid approach (76). Finally, MS can also provide a positive detection method. Although information available about the site of binding is usually more li ...
... several groups (7, 44, 53) have used crystallography to discover and develop fragments. Crystallography can also be combined with NMR to yield a hybrid approach (76). Finally, MS can also provide a positive detection method. Although information available about the site of binding is usually more li ...
Report Organelles in Blastocystis that Blur the
... same organelles is more suggestive of hydrogenosomal metabolism. To clarify the metabolic properties of the Blastocystis MLOs, we used 3330 clusters constructed from our EST data for a comparative BLAST search against the yeast and human mitochondrial proteomes [14, 15]. In addition, the KEGG Automa ...
... same organelles is more suggestive of hydrogenosomal metabolism. To clarify the metabolic properties of the Blastocystis MLOs, we used 3330 clusters constructed from our EST data for a comparative BLAST search against the yeast and human mitochondrial proteomes [14, 15]. In addition, the KEGG Automa ...
Interactome
In molecular biology, an interactome is the whole set of molecular interactions in a particular cell. The term specifically refers to physical interactions among molecules (such as those among proteins, also known as protein-protein interactions) but can also describe sets of indirect interactions among genes (genetic interactions). Mathematically, interactomes are generally displayed as graphs.The word ""interactome"" was originally coined in 1999 by a group of French scientists headed by Bernard Jacq. Though interactomes may be described as biological networks, they should not be confused with other networks such as neural networks or food webs.