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Genetic and biochemical identification of the
Genetic and biochemical identification of the

De Novo Pyrimidine Nucleotide Synthesis Mainly
De Novo Pyrimidine Nucleotide Synthesis Mainly

... by adding the nucleobase uracil (Chen and Slocum, 2008). In general, it is assumed that pyrimidine de novo synthesis is exclusively located in plastids with the exception of the reaction catalyzed by dihydroorotate dehydrogenase (DHODH) (Slocum, 2005). However, direct experimental evidence for this ...
De Novo Pyrimidine Nucleotide Synthesis Mainly
De Novo Pyrimidine Nucleotide Synthesis Mainly

... by adding the nucleobase uracil (Chen and Slocum, 2008). In general, it is assumed that pyrimidine de novo synthesis is exclusively located in plastids with the exception of the reaction catalyzed by dihydroorotate dehydrogenase (DHODH) (Slocum, 2005). However, direct experimental evidence for this ...
Sequence - BIOTEC - Biotechnology Center TU Dresden
Sequence - BIOTEC - Biotechnology Center TU Dresden

... n Archival databanks of biological information n DNA and protein sequences including annotations n Nucleic acid and protein structures including annotations n Protein expression patterns ...
High pKa variability of cysteine residues in structural databases and
High pKa variability of cysteine residues in structural databases and

... are very important, particularly for studies aimed to detect and describe reactive Cys residues in proteins; for example, the identification of specific redox targets in the cell (e.g. substrates of thiol-oxidoreductases) or prediction of new hot spots of oxidative stress. Different approaches have ...
Current understanding of fatty acid biosynthesis and the acyl carrier
Current understanding of fatty acid biosynthesis and the acyl carrier

Fatty acid
Fatty acid

... • In straight structures, H atoms on one strand can bond with OH groups on other strands • Parallel cellulose molecules held together this way are grouped into microfibrils, which form strong building materials for plants Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummi ...
A comparison of scoring functions for protein sequence
A comparison of scoring functions for protein sequence

PPt Chapter 5 - columbusisd.org
PPt Chapter 5 - columbusisd.org

... • In straight structures, H atoms on one strand can bond with OH groups on other strands • Parallel cellulose molecules held together this way are grouped into microfibrils, which form strong building materials for plants Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummi ...
Mutational analysis of exoribonuclease I from Saccharomyces
Mutational analysis of exoribonuclease I from Saccharomyces

... most in vivo and in vitro functions but confers a dominant negative growth inhibition when expressed at high levels. This growth inhibition is not due to the exonuclease function of the protein. To identify specific residues responsible for in vivo function, a screen was carried out for non-compleme ...
Optimizing cofactor availability for the production of recombinant
Optimizing cofactor availability for the production of recombinant

Sequence - BIOTEC - Biotechnology Center TU Dresden
Sequence - BIOTEC - Biotechnology Center TU Dresden

Products for Solid State NMR - Sigma
Products for Solid State NMR - Sigma

... 400 aa protein complex in membranes23. In these experiments, the judicious choice of the amino-acid labeling pattern was crucial to provide sufficient spectral resolution. It seems likely that such studies, together with the application of three – or even higher-dimensional ssNMR correlation experim ...
A Raman spectroscopic study of the interaction between nucleotides
A Raman spectroscopic study of the interaction between nucleotides

... polynucleotides poly(rA), poly(dA), poly(dT), poly(rU), and poly(rC), and with the oligonucleotides (dA), and (dA),, were recorded and interpreted. According to an analysis of the gp32 spectra with the reference intensity profiles of Alix and co-workers [M. Berjot, L. Marx, and A. J. P. Alix (1985) ...
Predicting DNA-binding sites of proteins from amino acid sequence
Predicting DNA-binding sites of proteins from amino acid sequence

... More recently, methods for precisely identifying proteinDNA contacts by coupling photochemical crosslinking with mass spectrometry have also been developed [9]. With increasing availability of protein sequence data, there is an urgent need for computational tools that can rapidly and reliably identi ...
Protein relative abundance patterns associated with sucrose
Protein relative abundance patterns associated with sucrose

... to catalog the functional potential of all microbial genes within a smaller subset of subjects. One of the key findings was that healthy sites from different people were broadly similar with respect to their functional profiles, even though there was extensive individual variation in their taxonomic ...


... i) Although the relative position of the TA bases is correct, the diagram contains at least three errors. Identify and correct three of these errors (3 pts). ii) Sketch the phosphate linkage that you would observe linking the T to the next base (2 pts) ii) Indicate the “Watson-Crick’ hydrogen bonds ...
PhoR, PhoP and MshC: Three essential proteins of Mycobacterium
PhoR, PhoP and MshC: Three essential proteins of Mycobacterium

... 1.1.1 Introduction to the Tuberculosis infectious process Tuberculosis (TB) is an infectious disease caused by the Mycobacterium tuberculosis (M.tb) bacillus. According to the World Health Organization, nearly onethird of the world’s population is infected with TB1. In 2012, 8.6 million new cases of ...
Summary - University of Amsterdam
Summary - University of Amsterdam

... reducingg potential. Interestingly, it was found that the targeting of Girl p proceeds in an unusual fashion,, since disrupting either of the two known protein import receptors for peroxisomal matrix proteinss (pex5p and pex7p) had no effect on the peroxisomal localization of Girl p. Inn recent year ...
Full-Text PDF
Full-Text PDF

... and have to be incorporated as D during protein expression. This is routinely done by growing E. coli cultures for protein expression in deuterated minimal media, such as M9, composed of stock solutions and components dissolved in D2 O [11]. For the highest level D incorporation (~99%) it is best to ...
Molecular identification of three Arabidopsis thaliana mitochondrial
Molecular identification of three Arabidopsis thaliana mitochondrial

... essential to several plant-specific processes such as photorespiration, C4 and CAM (crassulacean acid metabolism) photosynthesis, the utilization of carbon, nitrogen and lipid storage compounds during seed germination and the GABA (γ -aminobutyric acid) shunt. Because individual steps in these metab ...
A siderophore biosynthesis gene cluster from the fish
A siderophore biosynthesis gene cluster from the fish

... The GenBank/EMBL/DDBJ accession number for the sequences reported in this paper is AJ699306. ...
Partial Purification and Characterization of Three Flavonol
Partial Purification and Characterization of Three Flavonol

... to the hydroxyl group(s) of flavonols. This study (21) seemed to indicate that the enzymic synthesis of polysulfated flavonols is catalyzed by a family of position-specific STs (EC 2.8.2-). Recent studies (2, 9) indicated that Flaveria chloraefolia is a rich source of mono- and disulfate esters of q ...
12 Molecular Visualization of an Enzyme, Acetylcholinesterase
12 Molecular Visualization of an Enzyme, Acetylcholinesterase

... of the multi-drug resistance (MDR) efflux pump protein. This is the protein that is up-regulated by a riboswitch in response to antibiotics like tetracycline. The protein then pumps the antibiotics out of the bacteria allowing the cells continued growth. Because the MDR efflux pump eliminates many d ...
Participation of DDDD and KPAR
Participation of DDDD and KPAR

... enzyme was identified in a metagenomic dataset established from microbial community resides in the LCL environment. The metagenome-derived MerA enzyme (ATII-LCL MerA) has simple and limited alterations in its primary structure relative to that of an ortholog from uncultured soil bacterium. Both enzy ...
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Interactome



In molecular biology, an interactome is the whole set of molecular interactions in a particular cell. The term specifically refers to physical interactions among molecules (such as those among proteins, also known as protein-protein interactions) but can also describe sets of indirect interactions among genes (genetic interactions). Mathematically, interactomes are generally displayed as graphs.The word ""interactome"" was originally coined in 1999 by a group of French scientists headed by Bernard Jacq. Though interactomes may be described as biological networks, they should not be confused with other networks such as neural networks or food webs.
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