Report of attending the international CILIA 2016 in Amsterdam
... aspects of cilia functions in cell physiology and development are in progress and a growing number of laboratories is involved in this research area. This Cilia meeting 2016 attracted more than 370 registered participants and over 230 abstracts came from all around the world. During the meeting, man ...
... aspects of cilia functions in cell physiology and development are in progress and a growing number of laboratories is involved in this research area. This Cilia meeting 2016 attracted more than 370 registered participants and over 230 abstracts came from all around the world. During the meeting, man ...
Lecture 2 Sequence Alignment
... a gene duplication event (e.g. human α-globin and human β-globin, various versions of both ) ...
... a gene duplication event (e.g. human α-globin and human β-globin, various versions of both ) ...
Phylogenetic and evolutionary analyses of St. Louis encephalitis
... proximal strains are also genetically similar. The genome of SLEV is, like that of all members of the genus Flavivirus, a non-segmented, single-stranded, positive-sense RNA, approximately 11 kb in length. It encodes a single 3429 amino acid polyprotein, which is co- and post-translationally cleaved ...
... proximal strains are also genetically similar. The genome of SLEV is, like that of all members of the genus Flavivirus, a non-segmented, single-stranded, positive-sense RNA, approximately 11 kb in length. It encodes a single 3429 amino acid polyprotein, which is co- and post-translationally cleaved ...
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... (less frequently) or UUG (rarely). In all three cases, the protein starts with formylmethionine. The formyl group is removed after the first several amino acids have been incorporated, and in about half the cases, the methionine is also removed. 4. Note that the meaning of AUG and GUG is dependent o ...
... (less frequently) or UUG (rarely). In all three cases, the protein starts with formylmethionine. The formyl group is removed after the first several amino acids have been incorporated, and in about half the cases, the methionine is also removed. 4. Note that the meaning of AUG and GUG is dependent o ...
Full-Text - Academic Journals
... selected for virus purification. Sap inoculation of 100 C. sativus plantlets was done as previously described and young symptomatic leaves were harvested and used for virus isolation. Virus purification Virus purification followed the method described by Roossinck and White (1998). Freshly harvested ...
... selected for virus purification. Sap inoculation of 100 C. sativus plantlets was done as previously described and young symptomatic leaves were harvested and used for virus isolation. Virus purification Virus purification followed the method described by Roossinck and White (1998). Freshly harvested ...
Identification, cloning and sequence determination of genes specifying hexokinase A and B from yeast.
... and 3' ends of the HKB structural gene were calculated to be roughly 1170 nucleotides upstream and 210 downstream from the PstI site. Since a number of restriction sites had already been identified in these regions, they were used to obtain suitable DNA fragments from pBR328(EcoII) for dideoxy seque ...
... and 3' ends of the HKB structural gene were calculated to be roughly 1170 nucleotides upstream and 210 downstream from the PstI site. Since a number of restriction sites had already been identified in these regions, they were used to obtain suitable DNA fragments from pBR328(EcoII) for dideoxy seque ...
Tetracycline Antibiotics
... represent the invariant pharmacophore region of the molecule, where modifications are not tolerated without loss of antibiotic activity. ...
... represent the invariant pharmacophore region of the molecule, where modifications are not tolerated without loss of antibiotic activity. ...
Full Text
... some biochemical or physical constraints required for the structure or function of a protein. Thus, we require that amino acid groups be based on some evolutionary, biochemical, or physical property, such as volume, charge, or hydrophobicity. Using these groups, our method systematically explores po ...
... some biochemical or physical constraints required for the structure or function of a protein. Thus, we require that amino acid groups be based on some evolutionary, biochemical, or physical property, such as volume, charge, or hydrophobicity. Using these groups, our method systematically explores po ...
Insights into interactions between poly(ethylene glycol) and proteins
... interactions between the two measured species and negative B22 values describe attractive interactions between the measured species. It has been found that the second virial coefficient (B22) for the PEG - lysozyme interaction is lower (i.e. more favorable) than would be expected if the interaction ...
... interactions between the two measured species and negative B22 values describe attractive interactions between the measured species. It has been found that the second virial coefficient (B22) for the PEG - lysozyme interaction is lower (i.e. more favorable) than would be expected if the interaction ...
Glucose Polyester Biosynthesis. Purification and
... proteins from ConA and Mono P columns containing the same amount of disproportionation activity were loaded on a SDS-PAGE column. Intensities of the 34- and 24-kD bands were similar in these two samples (not shown), suggesting that they are likely candidates for the enzyme. To confirm the identity o ...
... proteins from ConA and Mono P columns containing the same amount of disproportionation activity were loaded on a SDS-PAGE column. Intensities of the 34- and 24-kD bands were similar in these two samples (not shown), suggesting that they are likely candidates for the enzyme. To confirm the identity o ...
pDsRed-Monomer Vector Information
... Note, however, that if you excise the DsRed-Monomer coding sequence using a restriction site in the 5' MCS, the resulting fragment will encode solely the DsRed-Monomer protein (i.e., without the additional amino acids that are expressed using the lac promoter). A Kozak consensus sequence is located ...
... Note, however, that if you excise the DsRed-Monomer coding sequence using a restriction site in the 5' MCS, the resulting fragment will encode solely the DsRed-Monomer protein (i.e., without the additional amino acids that are expressed using the lac promoter). A Kozak consensus sequence is located ...
Deciphering the molecular basis of the specificity of protein
... determine their amino acid preferences. Two datasets have been examined. Firstly, one composed of non-covalently bound carbohydrates ligands. The results of this analysis is compared to the second dataset, obtained from the study of the spatial vicinity of the monosaccharides that form the common st ...
... determine their amino acid preferences. Two datasets have been examined. Firstly, one composed of non-covalently bound carbohydrates ligands. The results of this analysis is compared to the second dataset, obtained from the study of the spatial vicinity of the monosaccharides that form the common st ...
World of Dairy Cattle Nutrition
... Most kids are taught to eat their fruits and vegetables. Why? Because they are good for you, and provide several essential vitamins and minerals for a healthy, balanced diet! It is just as important for a dairy cow to eat a balanced diet to live a long, healthy life. The basic difference between hum ...
... Most kids are taught to eat their fruits and vegetables. Why? Because they are good for you, and provide several essential vitamins and minerals for a healthy, balanced diet! It is just as important for a dairy cow to eat a balanced diet to live a long, healthy life. The basic difference between hum ...
Prying Into Prions - Colorado Parks and Wildlife
... concept, and include real data from recent research projects. ...
... concept, and include real data from recent research projects. ...
9. proteins i
... (enzymes) are largely responsible for determining the phenotype or properties of a cell in a particular environment. The total hereditary material of the cell or genotype dictates which type of protein the cell can produce. In fact, the proteins have built into their structure the information that i ...
... (enzymes) are largely responsible for determining the phenotype or properties of a cell in a particular environment. The total hereditary material of the cell or genotype dictates which type of protein the cell can produce. In fact, the proteins have built into their structure the information that i ...
Function The Structure of Avian CD5 Implies a Conserved
... There are several long stretches of sequence identity between species in the cytoplasmic region of CD5 sequences, including different motifs for phosphorylation (Fig. 2A). PKC has two potential target sites (SKK at position 368 and TPR at 394) for phosphorylation. The Prosite pattern search revealed ...
... There are several long stretches of sequence identity between species in the cytoplasmic region of CD5 sequences, including different motifs for phosphorylation (Fig. 2A). PKC has two potential target sites (SKK at position 368 and TPR at 394) for phosphorylation. The Prosite pattern search revealed ...
World Index of BioMolecular Visualization Resources
... variability; homology modelling; immunoglobulin; domains; color-coded multiple sequence alignment. This site contains extensive tutorials and lectures on antibody structure coupled with analyses of sequence variability in antibodies. The site also has a very nice set of Microsoft EXCEL spreadsheets ...
... variability; homology modelling; immunoglobulin; domains; color-coded multiple sequence alignment. This site contains extensive tutorials and lectures on antibody structure coupled with analyses of sequence variability in antibodies. The site also has a very nice set of Microsoft EXCEL spreadsheets ...
Two-hybrid screening
Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay.