PPTX - Bonham Chemistry
... • These alcohols have a more complex molecular structure than ethyl alcohol. • They provide an initial sweetness followed by a harsh after taste. • Formed by the metabolism of amino acids, so over modification during malting or mashing can lead to higher fusel alcohol levels. • They increase with fe ...
... • These alcohols have a more complex molecular structure than ethyl alcohol. • They provide an initial sweetness followed by a harsh after taste. • Formed by the metabolism of amino acids, so over modification during malting or mashing can lead to higher fusel alcohol levels. • They increase with fe ...
The Effect of Temperature on the Metabolism of
... found to differ in several respects (Table 2). The yield of organisms was greater at the lower growth temperature and slightly more nitrogen was utilized. In contrast, the ethanol production was less than half that observed with cultures grown at 38", despite the complete utilization of glucose. A f ...
... found to differ in several respects (Table 2). The yield of organisms was greater at the lower growth temperature and slightly more nitrogen was utilized. In contrast, the ethanol production was less than half that observed with cultures grown at 38", despite the complete utilization of glucose. A f ...
Proteomics of
... strongly predominate over their monomeric forms in boar seminal plasma. These interactions facilitate the formation of aggregated forms of proteins in the seminal plasma and probably the arrangement and remodeling of sperm coating proteins. It is interesting that the heparin-binding activity of aggr ...
... strongly predominate over their monomeric forms in boar seminal plasma. These interactions facilitate the formation of aggregated forms of proteins in the seminal plasma and probably the arrangement and remodeling of sperm coating proteins. It is interesting that the heparin-binding activity of aggr ...
Lecture 2 - cholesterol _CVS block
... transcription is suppressed and vice versa • Sterol Response Element (SRE) is a recognition sequence in the DNA • SREBP (SRE binding protein) binding to SRE is essential for transcription of this gene • SREBP cleavage activator protein (SCAP) is an intracellular cholesterol sensor ...
... transcription is suppressed and vice versa • Sterol Response Element (SRE) is a recognition sequence in the DNA • SREBP (SRE binding protein) binding to SRE is essential for transcription of this gene • SREBP cleavage activator protein (SCAP) is an intracellular cholesterol sensor ...
Defelipe, L.A, Dolghih E, Roitberg A.E., Nouzova M., Mayoral
... in a hydrophobic pocket formed by Ile-151, Ile-154, Tyr 155, Leu158, Val-221 and Val-224 (Fig. 4A). Docking results indicated a clear difference in the interaction of (10R)-JHA and (10S)-JHA with the hydrophobic pocket of the binding site of AeJHAMT. Specifically, while the epoxide ring of (10S)-JHA ...
... in a hydrophobic pocket formed by Ile-151, Ile-154, Tyr 155, Leu158, Val-221 and Val-224 (Fig. 4A). Docking results indicated a clear difference in the interaction of (10R)-JHA and (10S)-JHA with the hydrophobic pocket of the binding site of AeJHAMT. Specifically, while the epoxide ring of (10S)-JHA ...
BCH 305
... the degradation, sequencing, and chemical synthesis of peptides and proteins. In recent years, biochemists have developed an arsenal of reactions that are relatively specific to the side chains of particular amino acids. These reactions can be used to identify functional amino acids at ...
... the degradation, sequencing, and chemical synthesis of peptides and proteins. In recent years, biochemists have developed an arsenal of reactions that are relatively specific to the side chains of particular amino acids. These reactions can be used to identify functional amino acids at ...
Autophagosome expansion due to amino acid deprivation
... al., 1995), indicating that MDC specifically accumulates in these type of vacuoles. However, neither the regulation nor the requirements for MDC accumulation in autophagic vacuoles have been assessed. In order to analyze the molecular machinery involved in the autophagic process in mammalian cells, ...
... al., 1995), indicating that MDC specifically accumulates in these type of vacuoles. However, neither the regulation nor the requirements for MDC accumulation in autophagic vacuoles have been assessed. In order to analyze the molecular machinery involved in the autophagic process in mammalian cells, ...
Attenuation regulation of amino acid biosynthetic operons in
... (Table 1). Analysis of regulatory peptide open reading frames allowed for prediction of the regulating amino acids. Finally, analysis of positional clustering of genes and regulatory signals leads to identification of new candidate members of the biosynthetic pathways of branched chain amino acids, h ...
... (Table 1). Analysis of regulatory peptide open reading frames allowed for prediction of the regulating amino acids. Finally, analysis of positional clustering of genes and regulatory signals leads to identification of new candidate members of the biosynthetic pathways of branched chain amino acids, h ...
Recognition of Nucleic Acid Bases and Base
... nucleotide bases. To examine the repertoire of possible interactions, we have calculated geometrically plausible arrangements in which amino acids hydrogen bond to unpaired bases, such as those found in RNA bulges and loops, or to the 53 possible RNA base-pairs. We find 32 possible interactions that ...
... nucleotide bases. To examine the repertoire of possible interactions, we have calculated geometrically plausible arrangements in which amino acids hydrogen bond to unpaired bases, such as those found in RNA bulges and loops, or to the 53 possible RNA base-pairs. We find 32 possible interactions that ...
Ch 3 The Molecules of Life 20112012
... • A slight change in the primary structure of a protein affects its ability to function. ...
... • A slight change in the primary structure of a protein affects its ability to function. ...
Structure–function relationships of the variable - IMGT
... usually achieved by comparing the sequences of the variable domains of the selected rat or murine monoclonal antibody to human sequences and 3D structures available in databases. It should be emphasized that the three humanized antibodies were designed using the Kabat system for defining the CDRs [1 ...
... usually achieved by comparing the sequences of the variable domains of the selected rat or murine monoclonal antibody to human sequences and 3D structures available in databases. It should be emphasized that the three humanized antibodies were designed using the Kabat system for defining the CDRs [1 ...
THE BACTERIA toxin
... 1. Hemolysins - There are many different types of hemolysins but, in each case, the end result is lysis of the red blood cell with resultant anemia. 2. Leukocidins- Again, there are many different types of leukocidins, and some are specific for only one type of leukocyte. However, the end result in ...
... 1. Hemolysins - There are many different types of hemolysins but, in each case, the end result is lysis of the red blood cell with resultant anemia. 2. Leukocidins- Again, there are many different types of leukocidins, and some are specific for only one type of leukocyte. However, the end result in ...
Bacterial and Firefly Luciferase Genes in Transgenic Plants
... O,, anaesthetics, antibiotics, mutagens, etc., can be carried out in vivo by expression of luciferases in living cells [Hastings and Nealson, 19771. Cloning of luciferase genes opened the way to novel applications in molecular biology. Gene fusion is a general approach to study the temporal and spat ...
... O,, anaesthetics, antibiotics, mutagens, etc., can be carried out in vivo by expression of luciferases in living cells [Hastings and Nealson, 19771. Cloning of luciferase genes opened the way to novel applications in molecular biology. Gene fusion is a general approach to study the temporal and spat ...
Expression of the LXR Protein in Human Atherosclerotic Lesions
... Immunohistochemical analysis was performed as described previously.20 Human tissues were fixed for 1 day at room temperature in 10% formalin. The samples were sequentially dehydrated with an alcohol series and embedded in paraffin. Antigen retrieval was performed by heating the sections in an autocl ...
... Immunohistochemical analysis was performed as described previously.20 Human tissues were fixed for 1 day at room temperature in 10% formalin. The samples were sequentially dehydrated with an alcohol series and embedded in paraffin. Antigen retrieval was performed by heating the sections in an autocl ...
Identification of Aspartate- 184 as an Essential Residue in the
... an irreversible inhibitor of the catalytic subunit of CAMP-dependent protein kinase, and MgATP protected against inactivation [Toner-Webb, J., & Taylor, S. S. (1987) Biochemistry 26, 73711. This inhibition by DCCD indicated that an essential carboxyl group was present at the active site of the enzym ...
... an irreversible inhibitor of the catalytic subunit of CAMP-dependent protein kinase, and MgATP protected against inactivation [Toner-Webb, J., & Taylor, S. S. (1987) Biochemistry 26, 73711. This inhibition by DCCD indicated that an essential carboxyl group was present at the active site of the enzym ...
a curated database for protein phosphorylation sites in prokaryotes
... its critical roles in various cellular processes such as signal transduction. Thus, an integrative data resource of the prokaryotic phosphorylation will be useful for further analysis. In this study, we presented a curated database of phosphorylation sites in prokaryotes (dbPSP, Database URL: http:/ ...
... its critical roles in various cellular processes such as signal transduction. Thus, an integrative data resource of the prokaryotic phosphorylation will be useful for further analysis. In this study, we presented a curated database of phosphorylation sites in prokaryotes (dbPSP, Database URL: http:/ ...
Coenzyme A and Acyl Carrier Protein
... Acetyl-CoA derived via the citric acid cycle or from acetate via a CoA synthetase is of course the primary precursor for fatty acid sythases (see our web page on saturated fatty acids). In addition, short-chain acyl-CoAs including free CoA, acetyl-CoA and malonyl-CoA are well known regulators of met ...
... Acetyl-CoA derived via the citric acid cycle or from acetate via a CoA synthetase is of course the primary precursor for fatty acid sythases (see our web page on saturated fatty acids). In addition, short-chain acyl-CoAs including free CoA, acetyl-CoA and malonyl-CoA are well known regulators of met ...
HuaLi (215-221) - Asia Pacific Journal of Clinical Nutrition
... percentage of oleic acid can decrease low density lipoprotein-cholesterol (LDL-c) to high density lipoproteincholesterol ratio and so reduce the risk of cardiovascular disease7 and several cancers.8 Furthermore, the preferred ratio of n-6 to n-3 polyunsaturated fatty acids (PUFA) in soybean9-11 may ...
... percentage of oleic acid can decrease low density lipoprotein-cholesterol (LDL-c) to high density lipoproteincholesterol ratio and so reduce the risk of cardiovascular disease7 and several cancers.8 Furthermore, the preferred ratio of n-6 to n-3 polyunsaturated fatty acids (PUFA) in soybean9-11 may ...
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... their corresponding non-protein coding genes in the chromosome. The ribosomal proteins are translated from mRNAs copied from their protein coding genes. ...
... their corresponding non-protein coding genes in the chromosome. The ribosomal proteins are translated from mRNAs copied from their protein coding genes. ...
Biochemical studies on a versatile esterase that is most catalytically
... Because the active clone with CN1E1 actively hydrolysed naphthalene carboxylic acid methyl ester on an agar plate (Fig. 1), we further tested and quantified hydrolysis for the pure enzyme using this and other model PAH esters; the pH-indicator protocol (at 40°C and pH 8.0) used to examine hydrolysis ...
... Because the active clone with CN1E1 actively hydrolysed naphthalene carboxylic acid methyl ester on an agar plate (Fig. 1), we further tested and quantified hydrolysis for the pure enzyme using this and other model PAH esters; the pH-indicator protocol (at 40°C and pH 8.0) used to examine hydrolysis ...
Review Ribosome-independent Peptide Synthesis in Nature and
... (PPi). The other is aminoacylphosphate, which is formed by the following reaction; amino acid + ATP → aminoacylphosphate + ADP. Peptide-bond forming activities taking aminoacyl-AMP are represented by the ribosomal system and nonribosomal peptide synthetase. In the ribosome system, an aminoacyl resid ...
... (PPi). The other is aminoacylphosphate, which is formed by the following reaction; amino acid + ATP → aminoacylphosphate + ADP. Peptide-bond forming activities taking aminoacyl-AMP are represented by the ribosomal system and nonribosomal peptide synthetase. In the ribosome system, an aminoacyl resid ...
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... their corresponding non-protein coding genes in the chromosome. The ribosomal proteins are translated from mRNAs copied from their protein coding genes. ...
... their corresponding non-protein coding genes in the chromosome. The ribosomal proteins are translated from mRNAs copied from their protein coding genes. ...
Engineering carbonic anhydrase for highly selective ester hydrolysis Gunnar Höst
... The main part of this thesis describes results from protein engineering experiments, in which the catalytic activity of the enzyme human carbonic anhydrase II (HCAII) is engineered by mutagenesis. This enzyme, which catalyzes the interconversion between CO2 and HCO3- in the body, also has the abilit ...
... The main part of this thesis describes results from protein engineering experiments, in which the catalytic activity of the enzyme human carbonic anhydrase II (HCAII) is engineered by mutagenesis. This enzyme, which catalyzes the interconversion between CO2 and HCO3- in the body, also has the abilit ...
Two-hybrid screening
Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay.