Engineering carbonic anhydrase for highly selective ester hydrolysis Gunnar Höst
... The main part of this thesis describes results from protein engineering experiments, in which the catalytic activity of the enzyme human carbonic anhydrase II (HCAII) is engineered by mutagenesis. This enzyme, which catalyzes the interconversion between CO2 and HCO3- in the body, also has the abilit ...
... The main part of this thesis describes results from protein engineering experiments, in which the catalytic activity of the enzyme human carbonic anhydrase II (HCAII) is engineered by mutagenesis. This enzyme, which catalyzes the interconversion between CO2 and HCO3- in the body, also has the abilit ...
757 (Agus Kurnia)ok
... contributed to the thermostability (Harris et al. 1997). Moreover, the hydrophobic interaction at the surface of a protein has also been reported (Van den Burg et al. 1994; Funahashi et al. 2000) to stabilize the protein. Amino acid residues at the position of 222 were conserved in all bacterial xyl ...
... contributed to the thermostability (Harris et al. 1997). Moreover, the hydrophobic interaction at the surface of a protein has also been reported (Van den Burg et al. 1994; Funahashi et al. 2000) to stabilize the protein. Amino acid residues at the position of 222 were conserved in all bacterial xyl ...
Evidence of cytotoxic activity against mammalian red blood cell of
... distinguished from cellular lytic activity suggesting that the studied fraction have cytotoxic activity against erythrocytes because was partial overlapped with the 20 kDa equinotoxin. In this study a low molecular weight hemolysin from acid tissue tentacle extracts of sea anemone Actinia equina was ...
... distinguished from cellular lytic activity suggesting that the studied fraction have cytotoxic activity against erythrocytes because was partial overlapped with the 20 kDa equinotoxin. In this study a low molecular weight hemolysin from acid tissue tentacle extracts of sea anemone Actinia equina was ...
24g protein per serving
... This matter is both an issue of biochemistry as well as thermodynamics. Regarding the biochemistry of the issue, we know that goat milk has a greater amount of essential fatty acids such as linoleic and arachidonic acid than cow milk as well as significantly greater amounts of vitamin B-6, vitamin A ...
... This matter is both an issue of biochemistry as well as thermodynamics. Regarding the biochemistry of the issue, we know that goat milk has a greater amount of essential fatty acids such as linoleic and arachidonic acid than cow milk as well as significantly greater amounts of vitamin B-6, vitamin A ...
Biochemical Oscillations
... (either its affinity for substrates or its rate of converting bound substrates into products). Such enzymes are called “allosteric” because in addition to substrate-binding sites, they have “other sites” for binding regulatory molecules that either activate or inhibit the enzyme. Allosteric proteins ...
... (either its affinity for substrates or its rate of converting bound substrates into products). Such enzymes are called “allosteric” because in addition to substrate-binding sites, they have “other sites” for binding regulatory molecules that either activate or inhibit the enzyme. Allosteric proteins ...
Substrate recognition by nonribosomal peptide
... L-Phe-, L-Glu- and L-Asp-activating domains have been mutated, using this method, to domains activating preferentially L-Leu, L-Gln and L-Asn, respectively, as evaluated by the ATP-pyrophosphate exchange assay. Because no kinetic parameters could be determined for the activation of the noncognate am ...
... L-Phe-, L-Glu- and L-Asp-activating domains have been mutated, using this method, to domains activating preferentially L-Leu, L-Gln and L-Asn, respectively, as evaluated by the ATP-pyrophosphate exchange assay. Because no kinetic parameters could be determined for the activation of the noncognate am ...
PDF file
... form of GST-PIR1 containing only the catalytic domain (residues 1–206) was expressed in bacteria and purified in a manner similar to that of the full-length protein. When expressed on a molar basis, the specific activity of GST-PIR1 (1–206) toward p-nitrophenyl phosphate and the best protein substra ...
... form of GST-PIR1 containing only the catalytic domain (residues 1–206) was expressed in bacteria and purified in a manner similar to that of the full-length protein. When expressed on a molar basis, the specific activity of GST-PIR1 (1–206) toward p-nitrophenyl phosphate and the best protein substra ...
INTRODUCTION - international journal of advances in
... external signals are transmitted to the cell with the help of intracellular free Ca2+. The resting [Ca2+]i is regulated at three different Levels: 1) At cell membrane; 2) At the intracellular Ca2+ pools in the cytoplasm; and 3) By Ca2+ -binding proteins in the cytoplasm and the nucleus. First, there ...
... external signals are transmitted to the cell with the help of intracellular free Ca2+. The resting [Ca2+]i is regulated at three different Levels: 1) At cell membrane; 2) At the intracellular Ca2+ pools in the cytoplasm; and 3) By Ca2+ -binding proteins in the cytoplasm and the nucleus. First, there ...
SM-Day 1
... … includes no information about which kinds of changes are more likely to occur than other kinds of changes (Ex: hydrophobic amino acids, transition bias in DNA mutation). …provides only a “back-of-the envelope” model of evolution. ...
... … includes no information about which kinds of changes are more likely to occur than other kinds of changes (Ex: hydrophobic amino acids, transition bias in DNA mutation). …provides only a “back-of-the envelope” model of evolution. ...
A metabolic link to skeletal muscle wasting and regeneration
... 2006). The mTORC1 signaling pathway integrates a wide variety of extra- and intracellular signals, including insulin (and its related growth factors), nutrient (glucose and amino acids) availability, and cellular energy status to regulate protein synthesis, autophagy, cell growth and metabolism (Lap ...
... 2006). The mTORC1 signaling pathway integrates a wide variety of extra- and intracellular signals, including insulin (and its related growth factors), nutrient (glucose and amino acids) availability, and cellular energy status to regulate protein synthesis, autophagy, cell growth and metabolism (Lap ...
... of Krebs buffer containing 20 mmol/L HEPES, 5 mmol/L D-glucose, 0.3 mmol/L NH4Cl, 5 µL of 20 U/mL insulin, and 0, 0.5 or 2 L-leucine plus L-[1-14C]- or L-[U-14C]-labeled leucine, and other amino acids at physiological concentrations found in plasma (36). Values, expressed as nmol/106 cells per 2h un ...
Zaenab Aljassim thesis-6_1
... my thesis, dissertation, or project report in whole or in part in all forms of media, now or for the duration of my copyright ownership. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also reserve the right to use in future works (such as articl ...
... my thesis, dissertation, or project report in whole or in part in all forms of media, now or for the duration of my copyright ownership. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also reserve the right to use in future works (such as articl ...
The risks and benefits of feeding intact male swine in the United
... suggeststhat for this age group, the maximumingestivecapacitylies between2.0-2.2kg per day,and that diets of 3350-3465 kcal DE(14070-14550kJ)are required to maximize protein and fat growth. On a typical westernCanadian diet which lacks corn but is based on wheat, barley, and soybean meal as the grai ...
... suggeststhat for this age group, the maximumingestivecapacitylies between2.0-2.2kg per day,and that diets of 3350-3465 kcal DE(14070-14550kJ)are required to maximize protein and fat growth. On a typical westernCanadian diet which lacks corn but is based on wheat, barley, and soybean meal as the grai ...
Effects of 6-Thioguanine on RNA Biosynthesis in Regenerating Rat
... shown that 6-TG acts in the G, phase of the cell cycle of H.Ep.2 cells to delay the G1- to S-phase transition; the biochemical alteration responsible for this action is un known but would appear to be unrelated to incorporation of the analog into DNA. GTP is required at several stages of the transla ...
... shown that 6-TG acts in the G, phase of the cell cycle of H.Ep.2 cells to delay the G1- to S-phase transition; the biochemical alteration responsible for this action is un known but would appear to be unrelated to incorporation of the analog into DNA. GTP is required at several stages of the transla ...
insulin history
... that have been joined together in a single DNA molecule. Could this process be used to produce human insulin in bacterial cells? Could large enough quantities be produced so that it could be used to treat diabetes? Before these questions could be answered, several key developments in molecular biolo ...
... that have been joined together in a single DNA molecule. Could this process be used to produce human insulin in bacterial cells? Could large enough quantities be produced so that it could be used to treat diabetes? Before these questions could be answered, several key developments in molecular biolo ...
SILK-BASED DELIVERY OF BIOACTIVE MOLECULES
... Processed under ambient aqueous conditions to avoid loss of bioactivity of drugs to be delivered. Less inflammatory than other common biodegradable polymers such as poly(lactide) and collagen. Proccessability into films, hydrogels, nano-fibres, and three-dimensional scaffolds. Degradation ra ...
... Processed under ambient aqueous conditions to avoid loss of bioactivity of drugs to be delivered. Less inflammatory than other common biodegradable polymers such as poly(lactide) and collagen. Proccessability into films, hydrogels, nano-fibres, and three-dimensional scaffolds. Degradation ra ...
CH2O -OCH CH2O- - f.a. #1 f.a.#2 f.a.#3 f.a. = fatty acid.
... must not be absolutely protective for it must allow selective COMMUNICATION with the exterior so that nutrients can enter and waste products leave-it is not totally impermeable. Macroscopic material can enter via phagocytosis (solids) or pinocytosis (liquids)-remember Amoeba-while material in soluti ...
... must not be absolutely protective for it must allow selective COMMUNICATION with the exterior so that nutrients can enter and waste products leave-it is not totally impermeable. Macroscopic material can enter via phagocytosis (solids) or pinocytosis (liquids)-remember Amoeba-while material in soluti ...
RTS™ pIVEX E. coli His-tag 2nd Generation Vector Set Manual
... Selecting the cloning strategy In general, the Nco I/Sma I restriction site combination is recommended for cloning into pIVEX vectors, since this approach provides optimal flexibility to switch into all available pIVEX vectors and normally results in good expression efficiencies. Once a PCR fragment ...
... Selecting the cloning strategy In general, the Nco I/Sma I restriction site combination is recommended for cloning into pIVEX vectors, since this approach provides optimal flexibility to switch into all available pIVEX vectors and normally results in good expression efficiencies. Once a PCR fragment ...
Cloning and characterization of the Xenopus laevis p8 gene
... it is likely to be a transcription factor. The DNAbinding activity of human p8 is increased with phosphorylation (Encinar et al. 2001) and, while we could not identify any putative phosphorylation sites on Xp8, there were a couple of well-conserved amino acids outside the bHLH region (Fig. 2a) that ...
... it is likely to be a transcription factor. The DNAbinding activity of human p8 is increased with phosphorylation (Encinar et al. 2001) and, while we could not identify any putative phosphorylation sites on Xp8, there were a couple of well-conserved amino acids outside the bHLH region (Fig. 2a) that ...
And Information Catalog
... Ingredients Guarantee begins with Max Muscle Sports Nutrition’s focus on quality products and our commitment to sourcing, purchasing and controlling raw materials. The MMSN Quality Control Department has implemented comprehensive and thorough process specifications and controls. The first quality co ...
... Ingredients Guarantee begins with Max Muscle Sports Nutrition’s focus on quality products and our commitment to sourcing, purchasing and controlling raw materials. The MMSN Quality Control Department has implemented comprehensive and thorough process specifications and controls. The first quality co ...
Report of attending the international CILIA 2016 in Amsterdam
... aspects of cilia functions in cell physiology and development are in progress and a growing number of laboratories is involved in this research area. This Cilia meeting 2016 attracted more than 370 registered participants and over 230 abstracts came from all around the world. During the meeting, man ...
... aspects of cilia functions in cell physiology and development are in progress and a growing number of laboratories is involved in this research area. This Cilia meeting 2016 attracted more than 370 registered participants and over 230 abstracts came from all around the world. During the meeting, man ...
0 - Microbiology
... markers into recipient cells. The isolation of many more Hfr strains with different origines led rapidly to the construction of increasingly complete linkage maps, such as that compiled by Taylor & Trotter (1972), for markers located on the circular chromosome of E. coli. Since, under favourable con ...
... markers into recipient cells. The isolation of many more Hfr strains with different origines led rapidly to the construction of increasingly complete linkage maps, such as that compiled by Taylor & Trotter (1972), for markers located on the circular chromosome of E. coli. Since, under favourable con ...
JNK1 plays an important part in this process provides an
... conserved aspartate residue (Figure 1, arrow) and a few key residues in the catalytic center suggest that all pseudouridylases, RNA-guided or not, share a conserved mode of catalysis. In comparison to Cbf5, the catalytic domains of TruB and other pseudouridylases contain additional segments that are ...
... conserved aspartate residue (Figure 1, arrow) and a few key residues in the catalytic center suggest that all pseudouridylases, RNA-guided or not, share a conserved mode of catalysis. In comparison to Cbf5, the catalytic domains of TruB and other pseudouridylases contain additional segments that are ...
Two-hybrid screening
Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay.