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... A side chain, also called an R group ...
4 Classes of Large Biological Molecules Carbohydrates Lipids
4 Classes of Large Biological Molecules Carbohydrates Lipids

... Compose 50% of the dry mass of most cells Some are enzymes; others play roles in structural support, storage, transport, cellular communications, movement, and defense Enzyme Acts as cellular catalyst: selectively speed up reaction w/o being consumed Substance enzyme attaches to is called a substrat ...
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Document

... Wobble Position Some tRNA molecules can recognize and pair with more than one specific codon. Base-pairing between the 3’ base of a codon and 5’ base of an anticodon is not always exact. ...
Protein modification and trafficking
Protein modification and trafficking

... asparagine residue of a target protein having the sequence Asn-x-Ser/Thr, where X is any amino acid. ...
level two biology: gene expression
level two biology: gene expression

... translation by stating the result of each process and why each process is necessary for protein synthesis. I can differentiate between transcription and translation by explaining which occurs first and why and where each process occurs in a cell. I can show that I know the difference between mRNA, t ...
Leukemia Inhibitory Factor (LIF)
Leukemia Inhibitory Factor (LIF)

... Recombinant: Expressed in E. coli Purity: >95% as determined by SDS-PAGE, RP-HPLC and FPLC. Formulation: Lyophilized after extensive dialysis against PBS. Specific Activity: The ED50 was determined by the M1 cell differentiation assay is < 0.01 ng/ml, corresponding to a specific activity of 1.0 x 10 ...
structural organization
structural organization

SI Practice exam 2
SI Practice exam 2

Lipids - AHSbogna
Lipids - AHSbogna

... in snack foods. What makes olestra unique is that it is not just a fat substitute, but it is a fat. It has all of the same properties of fat when it is used in cooking, but the body can not absorb it so it has no calories. At first glance, olestra sounds like the answer to the prayers of many dieter ...
Lecture 3
Lecture 3

... • Refers to the organization of subunits in a protein with multiple subunits, may be identical or different.Subunits have a defined arrangement held together by weak, noncovalent interactions (hydrophobic, H bonds,ionic bonds) .There are two major categories of proteins with quaternary structure - ...
Gene Duplication in the Mo-Fe Protein of Nitrogenase
Gene Duplication in the Mo-Fe Protein of Nitrogenase

... • Constructed a phylogenetic tree of the Mo-Fe protein of nitrogenase from published nucleic acid data. • Tried to get a more coherent tree by eliminating some of the sequences. • Tree still not revealing. • Mistake: using nucleotides, switched to amino acid sequences for the alpha and beta chains. ...
From Genes to Proteins What do genes code for?
From Genes to Proteins What do genes code for?

... How is Transcription Started? Transcription Factors • Cell signal to transcribe • Bind to promoter region • The “TATA Box” ...
Chapter 3
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C - Bioinformatics Research Center

... • Hydrogen bonding holds the two strands together • 2 bonds between A and T • 3 bonds between G and C ...
The Development and Evaluation of Highly Specific
The Development and Evaluation of Highly Specific

... containing G-protein-coupled receptor 5 (LGR5) is one of the biomarkers specifically expressed on colon cancer stem cells. LGR5 can be activated by extracellular Wnt signaling molecules and this results in cancer development. Therefore, it is critical to develop a LGR5 antibody with great sensitivit ...
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... 18. Ganglion is the junction between 2 neuron ....................................... 19. AIDS is a genetic disease which can be transmitted to the next generation 20. Glycolysis is occurred in the cytoplasm and need the O2 . 21. The genotype of an organism refers to its appearance ……. 22. Glycolysi ...
Name
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... the questions completely before answering. If you are unclear about a question, please ask me. Part A: Multiple Choice: Answer with the best choice. Make sure that you clearly circle the correct answer and erase any erroneous marks. (2.5 pts each) 1. The degeneracy of the genetic code refers to the ...
Chapter5 The Structure and Functionof Macromolecules Discussion
Chapter5 The Structure and Functionof Macromolecules Discussion

... 13. Explain how a peptide bond forms between two amino acids. 14. List and describe the four major components of an amino acid. Explain how amino acids may be grouped according to the physical and chemical properties of the R group. 15. Explain what determines protein conformation and why it is impo ...
Nerve activates contraction
Nerve activates contraction

... • Produce water and carbon dioxide ...
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... Foldit is a revolutionary new computer game enabling you to contribute to important scientific research. Join this free online game and help us predict the folds of unsolved proteins as well as designing new proteins to cure diseases. We’re collecting data to find out if humans' pattern-recognition ...
Protein Structure Prediction not a trivial matter
Protein Structure Prediction not a trivial matter

... stability is not fully understood The primary sequence may not fully specify the tertiary structure (chaperones have the ability to induce proteins to fold in ...
Lecture 2- protein structure
Lecture 2- protein structure

... Striking examples of protein folding-related diseases are prion diseases, such as Creutzfeldt-Jacob disease (in humans), and mad cow disease (in cows), and scrapie (in sheep). Pathological conditions can result if a brain protein known to as prion protein (PrP) is misfolded into an incorrect form ca ...
Comparative Proteomics Kit I: Protein Profiler Module
Comparative Proteomics Kit I: Protein Profiler Module

... Can biomolecular evidence be used to determine evolutionary relationships? ...
Protein folding
Protein folding

... isomerase) is by far the most common tertiary fold. It is estimated that 10% of all known enzymes have this supersecondary structure. The members of this large family of proteins catalyze very different reactions. Currently, there are 85 enzymes in the TIM database including oxido/reductases, hydrol ...
Appendices Enzyme Endurance Review of Protein Structure Great
Appendices Enzyme Endurance Review of Protein Structure Great

... (green) bound. http://chemistry.umeche.maine.edu The three-dimensional structure of many proteins has evolved so that the binding of a small ligand can induce a significant change in protein shape. Most enzymes are allosteric proteins that can exist in two conformations that differ in catalytic acti ...
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Two-hybrid screening



Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay.
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