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DNA Replication and Protein Synthesis-New
DNA Replication and Protein Synthesis-New

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In silico Structural Biology of Signaling Proteins - Q-bio

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... – all proteins can be denatured – some proteins are inherently disordered (i.e. lack a regular structure) – some proteins get folding help from chaperones – there are various mechanisms through which the conformation of a protein can be changed in vivo – post-translational modifications such as phos ...
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Bacillus subtilis

... Escherichia coli is grown in a medium containing a mixture of glucose and lactose. During the first few hours the bacteria divide exponentially, using the glucose as the carbon and energy source. When the glucose is used up there is a brief lag period while the lac genes are switched on before the b ...
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Two-hybrid screening



Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay.
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