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Teacher`s Guide - Cornell Science Inquiry Partnerships
Teacher`s Guide - Cornell Science Inquiry Partnerships

... computational tools are being developed and employed to analyze gene and protein sequence data. Bioinformatic tools can facilitate lab-based experiments, which in turn validate or challenge the initial computational analyses. It is important to recognize that bioinformatic tools can only predict res ...
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... 12. In cells, NADH serves as a carrier of which of the following? a. Electrons b. Protons c. Hydroxyl groups d. Methyl groups e. Phosphoryl groups 13. Which of the following enzyme complexes catalyzes the reduction of oxygen to water during oxidative phosphorylation? a. ATP synthase b. Cytochrome c ...
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Central dogma of molecular biology

... Eventually, this mature mRNA finds its way to a ribosome, where it is translated. In prokaryotic cells, which have no nuclear compartment, the process of transcription and translation may be linked together. In eukaryotic cells, the site of transcription (the cell nucleus) is usually separated from ...
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Two-hybrid screening



Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay.
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