Substrate Metabolism – Rest vs Stress
... - rest = basal metabolic rate + minimal exercise - major stress = 50% burn - aim = to preserve plasma glucose levels for brain metabolism. REST - least expensive form of energy production utilized: carbohydrate -> fat -> protein in decreasing ratios. Carbohydrate Sources ...
... - rest = basal metabolic rate + minimal exercise - major stress = 50% burn - aim = to preserve plasma glucose levels for brain metabolism. REST - least expensive form of energy production utilized: carbohydrate -> fat -> protein in decreasing ratios. Carbohydrate Sources ...
Chapter 3: The Chemistry of Organic Molecules
... it to lose its conformation and hence its ability to function. If the denatured protein remains dissolved, it can often renature when the chemical and physical aspects of its environment are restored to normal. ...
... it to lose its conformation and hence its ability to function. If the denatured protein remains dissolved, it can often renature when the chemical and physical aspects of its environment are restored to normal. ...
Protein Synthesis
... Thymine (T), and Cytosine (C) which always bonds with Guanine (G). Each stand of DNA is complementary to the other. ...
... Thymine (T), and Cytosine (C) which always bonds with Guanine (G). Each stand of DNA is complementary to the other. ...
Topic 3 The Chemistry of Life - wfs
... 6. It is at the ribosomes where the process of translation occurs. Translation is the process that leads to the formation of polypeptides, proteins. 7. In the cytoplasm tRNA molecules contain anticodons. The tRNA anticodons pair with the mRNA codons through base pairing. Because each tRNA with a par ...
... 6. It is at the ribosomes where the process of translation occurs. Translation is the process that leads to the formation of polypeptides, proteins. 7. In the cytoplasm tRNA molecules contain anticodons. The tRNA anticodons pair with the mRNA codons through base pairing. Because each tRNA with a par ...
15-25 kD
... – Genus – Species • Traditional classification based upon traits: – Morphological – Behavioral ...
... – Genus – Species • Traditional classification based upon traits: – Morphological – Behavioral ...
LectureIV
... • 1) Engineered macromolecules could have experimental use as experimental tools, or for development and production of therapeutics • 2) During the process of said engineering, new techniques are developed which expand options available to research community as whole • 3) By approaching macromolecul ...
... • 1) Engineered macromolecules could have experimental use as experimental tools, or for development and production of therapeutics • 2) During the process of said engineering, new techniques are developed which expand options available to research community as whole • 3) By approaching macromolecul ...
Chapter 8.4, 8.5, 8.6, 8.7 Study Guide Key terms: Ribonucleic acid
... 1. Why do cells regulate gene expression? 2. What happens to the information on a DNA molecule during transcription? 3. What are repressor proteins and where do they bind? 4. mRNA leaves the nucleus and enters the cytoplasm (with or without) a complete set of both introns and exons. (please circle t ...
... 1. Why do cells regulate gene expression? 2. What happens to the information on a DNA molecule during transcription? 3. What are repressor proteins and where do they bind? 4. mRNA leaves the nucleus and enters the cytoplasm (with or without) a complete set of both introns and exons. (please circle t ...
mAb SAC1 INVESTIGATOR Name Zaven Kaprielian Address Albert
... 100 kDa in transmembrane protein Characterization Immunoprecipitation + (detergent-soluble membrane/cytoskeletal fractions derived from E11 rat spinal cord) Immunoblotting ...
... 100 kDa in transmembrane protein Characterization Immunoprecipitation + (detergent-soluble membrane/cytoskeletal fractions derived from E11 rat spinal cord) Immunoblotting ...
Lecture 9
... • The amino acid side chains in globular proteins are distributed according to polarities. • Nonpolar residues (Val, Leu, Ile, Met, and Phe) occur in the interior of a protein. • Charged polar residues (Arg, Lys, His, Asp, Glu) are mostly located on the surface of a protein. • Uncharged polar residu ...
... • The amino acid side chains in globular proteins are distributed according to polarities. • Nonpolar residues (Val, Leu, Ile, Met, and Phe) occur in the interior of a protein. • Charged polar residues (Arg, Lys, His, Asp, Glu) are mostly located on the surface of a protein. • Uncharged polar residu ...
Introductory Biology Primer
... – Stop making gene B once you have enough – Make genes C1, C2, C3 simultaneously ...
... – Stop making gene B once you have enough – Make genes C1, C2, C3 simultaneously ...
A1988N971500002
... only natural that my interest turned to nicotinamide adenine dinucleotide (NAD) as a form of activated ADP-ribose. Use of this pyridine nucleotide as a substrate of ADP-ribosyl transferases represents a fas- ...
... only natural that my interest turned to nicotinamide adenine dinucleotide (NAD) as a form of activated ADP-ribose. Use of this pyridine nucleotide as a substrate of ADP-ribosyl transferases represents a fas- ...
From Gene to Protein
... refined to be one-gene-onepolypeptide hypothesis Crick – Central Dogma of Genetics • DNA RNA Protein ...
... refined to be one-gene-onepolypeptide hypothesis Crick – Central Dogma of Genetics • DNA RNA Protein ...
Structural Properties of Enzymes
... Molecules with larger Mr sediment faster such that the rate of sedimentation (v) can be used to determine the Mr, as long as certain other physical properties of the protein such as its diffusion constant (D, empirically determined in an analytical ultracentrifuge at very low centrifugal force), par ...
... Molecules with larger Mr sediment faster such that the rate of sedimentation (v) can be used to determine the Mr, as long as certain other physical properties of the protein such as its diffusion constant (D, empirically determined in an analytical ultracentrifuge at very low centrifugal force), par ...
ORGANIC CHEMISTRY 4 Types of Macromolecules
... 1. DNA (deoxyribonucleic acid) – double strand of genetic information 2. RNA (ribonucleic acid) – single strand copy of DNA used to build proteins Examples of nongenetic nucleotides - plays a major role in cell metabolism 1. ATP (adenosine triphosphate) – carries energy for cellular activities 2. Su ...
... 1. DNA (deoxyribonucleic acid) – double strand of genetic information 2. RNA (ribonucleic acid) – single strand copy of DNA used to build proteins Examples of nongenetic nucleotides - plays a major role in cell metabolism 1. ATP (adenosine triphosphate) – carries energy for cellular activities 2. Su ...
Proteins Multiple choice Proteins can be classified as Polyesters
... maltose. The pH of saliva is about 7, which is close to the optimum temperature of an enzyme. Amylase stops functioning when it enters the stomach which has a pH of 2. What happens to the enzyme on entering the stomach which would cause it to ...
... maltose. The pH of saliva is about 7, which is close to the optimum temperature of an enzyme. Amylase stops functioning when it enters the stomach which has a pH of 2. What happens to the enzyme on entering the stomach which would cause it to ...
Chemistry part 2
... amino acid sequence • Determined by the sequence of amino acids • Amino acids linked by peptide bonds • Chain is called polypeptide • Sequence proceeds from “Nterminus” to “C-terminus” • Amino acid sequence determined by DNA code ...
... amino acid sequence • Determined by the sequence of amino acids • Amino acids linked by peptide bonds • Chain is called polypeptide • Sequence proceeds from “Nterminus” to “C-terminus” • Amino acid sequence determined by DNA code ...
Glutamate Synthase - Blue Valley Schools
... species name for the protein sequence (in this first case, Zea mays). Then, return to the Baylor website and “copy” just the protein sequence from the converted data, and “paste” it on the line following the “>Zea mays” identifier. 6. After you have finished this species, complete steps 1 through 5 ...
... species name for the protein sequence (in this first case, Zea mays). Then, return to the Baylor website and “copy” just the protein sequence from the converted data, and “paste” it on the line following the “>Zea mays” identifier. 6. After you have finished this species, complete steps 1 through 5 ...
Protein Structure - E-Learning
... All proteins are made up of many amino acids joined by peptide bonds. Peptide bonds are strong bonds and are not easily disrupted. (dipeptide = two amino acids, polypeptide = several amino acids) Each protein has a complex and unique conformation, which is determined by the specific amino acids and ...
... All proteins are made up of many amino acids joined by peptide bonds. Peptide bonds are strong bonds and are not easily disrupted. (dipeptide = two amino acids, polypeptide = several amino acids) Each protein has a complex and unique conformation, which is determined by the specific amino acids and ...
GLYCOGEN – energy storage in ANIMALS • Stored as cytoplasmic
... EX: Sickle cell anemia mutation changes one amino acid in the sequence (glu → ala) Abnormal hemoglobin molecules crystallize; cause blood cells to become sickle shaped FACTORS AFFECTING CONFORMATION Folding occurs as protein is synthesized, but physical/chemical environment plays a role DENATURATION ...
... EX: Sickle cell anemia mutation changes one amino acid in the sequence (glu → ala) Abnormal hemoglobin molecules crystallize; cause blood cells to become sickle shaped FACTORS AFFECTING CONFORMATION Folding occurs as protein is synthesized, but physical/chemical environment plays a role DENATURATION ...
Protein Synthesis: A Real Adventure
... 1 The mRNA student will enter the nucleus and transcribe the DNA into mRNA. REMEMBER, THE DNA CANNOT LEAVE THE NUCLEUS! 2. The mRNA student takes the mRNA to the Ribosome (your desk).Each set of three letters represents a codon. 3. The tRNA student will search out the correct anti-codon sequence car ...
... 1 The mRNA student will enter the nucleus and transcribe the DNA into mRNA. REMEMBER, THE DNA CANNOT LEAVE THE NUCLEUS! 2. The mRNA student takes the mRNA to the Ribosome (your desk).Each set of three letters represents a codon. 3. The tRNA student will search out the correct anti-codon sequence car ...
Transcription Biology Review
... up amino acids – Helix-turn-Helix (Homeodomain) – Helix-loop-helix – Zinc Finger ...
... up amino acids – Helix-turn-Helix (Homeodomain) – Helix-loop-helix – Zinc Finger ...
DNA plasmids/cloning
... • Generally want high copy numbers, exception is where high level of expression of protein has a lethal affect on host, then want low copy number. • pBR322 derivatives generally low copy number • Allows ‘lethal protein’ to be expressed below lethal concentration – Can increase copy number by – culti ...
... • Generally want high copy numbers, exception is where high level of expression of protein has a lethal affect on host, then want low copy number. • pBR322 derivatives generally low copy number • Allows ‘lethal protein’ to be expressed below lethal concentration – Can increase copy number by – culti ...
Two-hybrid screening
Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay.