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English Version
English Version

... The Teaching and Learning Outline of Biochemistry (English Version) Chapter One Proteins Purpose and requirements: 1. Grasp of the molecular components of proteins and the chemical bonds linking amino acids in the proteins, the physio-chemical properties of amino acids. 2. Profound grasp of the key ...
104371_Macromolecule_Basics
104371_Macromolecule_Basics

... Proteins are made of a chain of amino acids (the monomer or “building block”)  Aminio acid chain is then folded, twisted, and otherwise contorted into a very specific 3-D shape ...
Mouse anti-D4-GDI - Thermo Fisher Scientific
Mouse anti-D4-GDI - Thermo Fisher Scientific

... (Rho, Rac, Cdc42) by inhibiting the dissociation of GDP, thereby maintaining GTPases in their inactive, cytosolic form. The Rho family of GTPases is involved in a diverse variety of cellular functions, including cell signaling, proliferation, cytoskeletal organization, and secretion.1-6 These protei ...
Protein Synthesis
Protein Synthesis

... • Exons = the code that is useful for transcripting into proteins • Introns = are not useful • An enzyme splices the introns, puts together the useful sections (exons) ...
32_operons2
32_operons2

... • Positive regulation of lac (CAP) • Practice applying the operon concept to predict: – the phenotypes of mutants – The characteristics of other operons ...
29_Metabolism of amino acids. Digestion of proteins
29_Metabolism of amino acids. Digestion of proteins

... Biological Value for Protein (BV) BV is : a measure for the ability of dietary protein to provide the essential amino acids required for tissue protein maintenance. • Proteins of animal sources (meat, milk, eggs) have high BV because they contain all the essential amino acids. • Proteins from plant ...
Helthy diet * myths and reality - Visegrad University Association
Helthy diet * myths and reality - Visegrad University Association

... Fat in the fire Fats are found in great abundance - in seeds, nuts, grains, fruits, and other plant sources. Most of the fat consumed is produced from animal products containing highly concentrated fat calories.  Except for the olives, avocados and nuts, the fat content in most plant products is r ...
DNA Code problerm
DNA Code problerm

... B. instability of the DNA molecule C. the ability of the same tRNA anticodon to recognize different codons D. the high mutability of certain genes 9. If you wanted to block transcription of a group of functionally related genes in a prokaryote, you could place an obstacle A. upstream of both the pro ...
Protein Synthesis Study Sheet
Protein Synthesis Study Sheet

... Draw a nucleic acid and label its parts. What is a polymer? What are proteins made from? Give 3 examples of proteins and describe their functions? What do enzymes do? What kind of a molecule is an enzyme? Draw an amino acid molecule. Draw 2 DIFFERENT proteins. What makes them different? What is the ...
Molecules of Life
Molecules of Life

... The food you eat, the silk a spider uses to make a web, the muscles in your body – all of these structures are made of macromolecules. Macromolecule is the term that biologists use for large molecules. There are four types of macromolecules that are important in biology: carbohydrates, lipids, prote ...
Mining the Human Genome Using Protein Structure Homology
Mining the Human Genome Using Protein Structure Homology

... Human Genome contains approximately 30-60 thousand genes Only 30-40% of these are classified into known function families Function of proteins needed to enable development of therapeutics ...
PPT File
PPT File

... its complexity and its lack of symmetry. The arrangement seems to be almost totally lacking in the kind of regularities which one instinctively anticipates, and it is more complicated than has been predicted by any theory of protein structure. - J. Kendrew, article in Naure, 1958 ...
Name
Name

... c. May have evolved from gibbons but not rats d. Is more closely related to humans than to rats e. May have evolved from rats but not from humans and gibbons 8. Proteins like hemoglobin and insulin have different structures because they have different ______________________, which is also known as t ...
Nutrition Physiology Proteins Lesson Plan 6-8
Nutrition Physiology Proteins Lesson Plan 6-8

... To figure out your protein needs, multiply your weight in pounds times 0.5 or you can just take your weight and divide by 2. For instance, a 70-pound (or 32-kilogram) kid should have about 35 grams of protein every day. If you only know your weight in kilograms, you need about 1 gram of protein each ...
A gene fusion consisting of 960 base pairs of 5`
A gene fusion consisting of 960 base pairs of 5`

... was prepared from the recombinant M13mp8 phage containing the appropriate insert. This template was annealed with the phosphorylated synthetic oligonucleotide of 24 bases in length. This primer-template intermediate was subjected to extension and ligation reaction at 23"C for 2 hours in the presence ...
38 Physiology of metabolism and energy
38 Physiology of metabolism and energy

... vessels (called lymphatic's) near the intestine. These small vessels carry the reformed fat to the veins of the chest, and the blood carries the fat to liver and than to storage depots in different parts of the body. ...
CHEM523 Final Exam Possible
CHEM523 Final Exam Possible

AMINO ACID PROFILE
AMINO ACID PROFILE

... Sacha inchi, Sacha inchik, Inca inchi ORIGIN: ...
File - singhscience
File - singhscience

File
File

... Very seldom does a polypeptide chain stay as a straight molecule. Usually it folds on itself forming a three dimensional structure. The proteins' structure is usually a key to its function. Hydrogen bonding between the hydrogen on the amine group and oxygen on the carboxyl group allows for secondary ...
Lesson18_Cultivation of microorganism
Lesson18_Cultivation of microorganism

...  unsuitable for heat sensitive chemicals & many foods. B. Autoclaving/pressure canning  Temperatures above 100°C achieved by steam pressure  Sterilization in autoclave is used in biomedical or clinical laboratory. ...
Confocal Laser Scanning Microscopy
Confocal Laser Scanning Microscopy

... monooxygenase anti-sense probe (1), TRITC-labeled arginine vasopressin anti-sense probe (2), or both probes (3) showing co-localization (green and red co-localization appears yellow). From Grino & Zamora (1998) J.Histochem. Cytochem. 46:753-759. ...
91.510_ch8_part1
91.510_ch8_part1

... of a protein. Its size is often 10 to 20 amino acids. Simple motifs include transmembrane domains and phosphorylation sites. These do not imply homology when found in a group of proteins. PROSITE (www.expasy.org/prosite) is a dictionary of motifs (there are currently 1600 entries). In PROSITE, a pat ...
Computational Pharmacology
Computational Pharmacology

... • help with the information in the databases and infer information that is not provided directly by genomics and proteomics data: higher level information => piece together all available information 1. To get detailed picture of a molecular process (or disease) 2. From 1, to identify new protein tar ...
Gene Section RAD51L3 (RAD51 like 3 (S. cerevisiae)) -
Gene Section RAD51L3 (RAD51 like 3 (S. cerevisiae)) -

... mice have shown a dramatic increase in levels of chromosomal aberrations, most notably, chromatid and chromosome breaks that occur through unrepaired replication forks (Smiraldo et al., 2005; Hinz et al., 2006). Proteomic studies have identified an interaction between RAD51D with the SFPQ protein (R ...
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Two-hybrid screening



Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay.
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