Electrophoresis
... also binding processes with Ka values significantly greater than 107 M-1 can be ...
... also binding processes with Ka values significantly greater than 107 M-1 can be ...
INSILICO MODELING OF CAPSULAR POLYSACCHARIDE BIOSYNTHESIS PROTEIN STREPTOCOCCUS PNEUMONIAE LIGAND IDENTIFICATION
... Objective: Identifying a novel drug target in Streptococcus pneumoniae to treat pneumonia as a challenging task in the current era of Post genomics. Hence the ability to predict the drug target by an insilico method is an initiation towards the drug discovery pipeline. Methods: Strain G54 of Strepto ...
... Objective: Identifying a novel drug target in Streptococcus pneumoniae to treat pneumonia as a challenging task in the current era of Post genomics. Hence the ability to predict the drug target by an insilico method is an initiation towards the drug discovery pipeline. Methods: Strain G54 of Strepto ...
1 slide per page
... - can be bound to proteins to “anchor” them to membranes - examples of attaching of the lipids to the proteins: myristoylation, palmitoylation ...
... - can be bound to proteins to “anchor” them to membranes - examples of attaching of the lipids to the proteins: myristoylation, palmitoylation ...
Full Text
... The phosphorylation of a protein can affect functions and activities of proteins, including intrinsic biological activity, half-life, subcellular location, and docking with other proteins (Cohen, 2000). The occurrence of many phosphorylation sites in β-amylase 7-like protein support that it may be r ...
... The phosphorylation of a protein can affect functions and activities of proteins, including intrinsic biological activity, half-life, subcellular location, and docking with other proteins (Cohen, 2000). The occurrence of many phosphorylation sites in β-amylase 7-like protein support that it may be r ...
Practice Exam II
... c. Beef tallow d. Coconut oil 16. Glycolysis is the conversion of… a. Glycogen to fat b. Glycogen to protein c. Glucose to pyruvate d. Glucose to glycogen 17. What primary factor governs the quality of a food protein? a. Fat content b. Essential amino acid content c. Complex carbohydrate content d. ...
... c. Beef tallow d. Coconut oil 16. Glycolysis is the conversion of… a. Glycogen to fat b. Glycogen to protein c. Glucose to pyruvate d. Glucose to glycogen 17. What primary factor governs the quality of a food protein? a. Fat content b. Essential amino acid content c. Complex carbohydrate content d. ...
Complete nucleotide sequence and genome organization of a
... The cr-TMV 29K protein shows clear sequence similarity to the transport proteins (TPs) of tob~oviruses (Fig. 3). However, it contains essentially larger proportion of basic amino acids than any other known tobamovirus TP, reflected in its unusually high isoelectric point (pZ 9.76). Basing on the fiv ...
... The cr-TMV 29K protein shows clear sequence similarity to the transport proteins (TPs) of tob~oviruses (Fig. 3). However, it contains essentially larger proportion of basic amino acids than any other known tobamovirus TP, reflected in its unusually high isoelectric point (pZ 9.76). Basing on the fiv ...
PHS 398 (Rev. 9/04), Biographical Sketch Format Page
... characterization experiments this project demands. Focusing this powerful technology towards the task of identifying new interacting proteins and post-translational modifications will require technical and computational expertise, and biological insight. My graduate and post-graduate work in each of ...
... characterization experiments this project demands. Focusing this powerful technology towards the task of identifying new interacting proteins and post-translational modifications will require technical and computational expertise, and biological insight. My graduate and post-graduate work in each of ...
Substrate
... (#1096176 from Roche Applied Science) dissolved in maleic acid buffer (100 mM maleic acid, 150 mM NaCl, pH 7.5). Probe-target hybrids were detected by an enzyme-linked immunoassay specific to dioxigenin as described by the manufacturer. The results were recorded on a X-ray film. An ~8-kb HindIII fra ...
... (#1096176 from Roche Applied Science) dissolved in maleic acid buffer (100 mM maleic acid, 150 mM NaCl, pH 7.5). Probe-target hybrids were detected by an enzyme-linked immunoassay specific to dioxigenin as described by the manufacturer. The results were recorded on a X-ray film. An ~8-kb HindIII fra ...
Gene Section PLCB2 (phospholipase C, beta 2) Atlas of Genetics and Cytogenetics
... By analyzing the relationship between PLC-b2 levels and biological and clinic-pathological factors, it has been found that the expression of PLC-b2 strikingly correlates with histological grade, mitotic index and size of primary tumors. No differences in PLC-b2 amount were found in breast tumors tha ...
... By analyzing the relationship between PLC-b2 levels and biological and clinic-pathological factors, it has been found that the expression of PLC-b2 strikingly correlates with histological grade, mitotic index and size of primary tumors. No differences in PLC-b2 amount were found in breast tumors tha ...
Observed Rate of Bubble Formation Distance to Light Observations
... A nerve cell and a liver cell in the same organism contain different combinations of proteins. Which of these statements BEST explains why different cells are different proteins? The different cells in an organism contain different genes Each cell in an organism transcribes only the genes that cell ...
... A nerve cell and a liver cell in the same organism contain different combinations of proteins. Which of these statements BEST explains why different cells are different proteins? The different cells in an organism contain different genes Each cell in an organism transcribes only the genes that cell ...
Transcription - smithlhhsb121
... The interaction of tRNA and mRNA takes place in a ribosome Consists of two protein subunits and ribosomal RNA (rRNA) Within the ribosome are three binding sites ◦ P site (peptidyl-tRNA site) where the tRNA holding the polypepetide chain ◦ A site (aminoacyl-tRNA site) where next tRNA in line is held ...
... The interaction of tRNA and mRNA takes place in a ribosome Consists of two protein subunits and ribosomal RNA (rRNA) Within the ribosome are three binding sites ◦ P site (peptidyl-tRNA site) where the tRNA holding the polypepetide chain ◦ A site (aminoacyl-tRNA site) where next tRNA in line is held ...
Chapter-1-Structure-and-Variety-of-Protein
... More jobs of proteins. • FIBRINOGEN and PROTHROMBIN are proteins which protect the body by clotting the blood at wounds. • All membranes are made of proteins, • The cell has a CYTOSKELETON which is made of protein. E.g. Microvillus, spindle fibres, and cilia all keep their shape because of their pro ...
... More jobs of proteins. • FIBRINOGEN and PROTHROMBIN are proteins which protect the body by clotting the blood at wounds. • All membranes are made of proteins, • The cell has a CYTOSKELETON which is made of protein. E.g. Microvillus, spindle fibres, and cilia all keep their shape because of their pro ...
chapter 10
... d. the protein chain sends a signal through the nerve cells to the brain. ____ 21. In bacteria, a group of genes that code for functionally related enzymes, their promoter site, and the operator that controls them all function together as a(n) a. exon. c. operon. b. intron. d. ribosome. ____ 22. The ...
... d. the protein chain sends a signal through the nerve cells to the brain. ____ 21. In bacteria, a group of genes that code for functionally related enzymes, their promoter site, and the operator that controls them all function together as a(n) a. exon. c. operon. b. intron. d. ribosome. ____ 22. The ...
Essential amino acids
... The intake of the protein in food：the intake↑↑urea synthesis AGA：CPS I is an allosteric enzyme sensitive to activation by N-acetylglutamate（AGA） which is derived from glutamate and acetyl-CoA. All intermediate products accelerate the reaction Rate-limiting enzyme of urea cycle is argininosu ...
... The intake of the protein in food：the intake↑↑urea synthesis AGA：CPS I is an allosteric enzyme sensitive to activation by N-acetylglutamate（AGA） which is derived from glutamate and acetyl-CoA. All intermediate products accelerate the reaction Rate-limiting enzyme of urea cycle is argininosu ...
The Protein Truncation Test
... Detection and Primer Design The detection method for PTT products must be considered when designing primers for amplification (18). Typically, [35S]methionine is the label of choice but other labels such as [35S]cysteine and [3H]leucine could be used as well. Thus, the amplified segments should contai ...
... Detection and Primer Design The detection method for PTT products must be considered when designing primers for amplification (18). Typically, [35S]methionine is the label of choice but other labels such as [35S]cysteine and [3H]leucine could be used as well. Thus, the amplified segments should contai ...
File
... 22. Why is the shape of a protein important? _____________________________________________________________________________ _____________________________________________________________________________ 23. What are the 7 functions of proteins? 1. ________________ 2. _______________ 3. __________ 4. ...
... 22. Why is the shape of a protein important? _____________________________________________________________________________ _____________________________________________________________________________ 23. What are the 7 functions of proteins? 1. ________________ 2. _______________ 3. __________ 4. ...
Protein Structure Similarity
... A ligand is a molecule that binds to another molecule to form a larger compound. For proteins, this can have the effect of inhibiting the proteins function or catalyzing its activities. Therefore, ligands are important in drug design. Given a set of ligands that are known to have the same activity ( ...
... A ligand is a molecule that binds to another molecule to form a larger compound. For proteins, this can have the effect of inhibiting the proteins function or catalyzing its activities. Therefore, ligands are important in drug design. Given a set of ligands that are known to have the same activity ( ...
Conformational Analysis Protein Folding Protein Structure
... Profile-based methods for fold-recognition are based on idea that the physico-chemical properties of the amino acids of the target protein must 'fit' with the environment in which they are placed in the modelled structure Each amino acid of the target sequence is coded according to is properties, ...
... Profile-based methods for fold-recognition are based on idea that the physico-chemical properties of the amino acids of the target protein must 'fit' with the environment in which they are placed in the modelled structure Each amino acid of the target sequence is coded according to is properties, ...
w0506_tutorial8
... Can BLAST help us to predict its SS? 2. Use any secondary structure prediction method to predict the secondary structure of 1O8V and compare it to the solved structure. NOTICE! The secondary structure definition in PDB is given in a 7 letter code instead of 3 letter code (H, E, C). For comparison pu ...
... Can BLAST help us to predict its SS? 2. Use any secondary structure prediction method to predict the secondary structure of 1O8V and compare it to the solved structure. NOTICE! The secondary structure definition in PDB is given in a 7 letter code instead of 3 letter code (H, E, C). For comparison pu ...
Current Microbiology 40:
... Restriction enzymes were purchased from Boehringer-Mannheim (Montreal, QC) and used with the buffers supplied by the manufacturer. The digestions of plasmid DNA were carried out for 4 h or overnight, at 37°C, and the restriction fragments analyzed by agarose gel electrophoresis [15]. Preparation and ...
... Restriction enzymes were purchased from Boehringer-Mannheim (Montreal, QC) and used with the buffers supplied by the manufacturer. The digestions of plasmid DNA were carried out for 4 h or overnight, at 37°C, and the restriction fragments analyzed by agarose gel electrophoresis [15]. Preparation and ...
Practice Exam II answers
... weights from one another requires that you first make 100 mL of a 25% acrylamide:bisacrylamide stock that is 35:1. How much solid acrylamide and bisacrylamide will you need to weight out and dissolve in 100 mL? a). 0.7 grams acrylamide and 24.3 grams bisacrylamide. b). 24.3 grams acrylamide and 0.7 ...
... weights from one another requires that you first make 100 mL of a 25% acrylamide:bisacrylamide stock that is 35:1. How much solid acrylamide and bisacrylamide will you need to weight out and dissolve in 100 mL? a). 0.7 grams acrylamide and 24.3 grams bisacrylamide. b). 24.3 grams acrylamide and 0.7 ...
Antimicrobial Agents
... Interfere with microbe’s vital metabolic process that does not occur in host cells (inhibit microbial enzymes) Act by: a. Competitive inhibition – competes with essential substrate to act with microbial enzyme b. Noncompetitive inhibition – reacts directly with enzyme ...
... Interfere with microbe’s vital metabolic process that does not occur in host cells (inhibit microbial enzymes) Act by: a. Competitive inhibition – competes with essential substrate to act with microbial enzyme b. Noncompetitive inhibition – reacts directly with enzyme ...
1. Sequence analysis
... DOTPLOT. Compare the two sequences 1.seq and 2.seq by "dotplot" analysis. Run COMPARE with the output directed to DOTPLOT ( DOTPLOT uses the output from COMPARE to make a 2D plot) Identification of repeats with DOTPLOT. COMPARE may be used to identify repeats in a sequence. Analyze the sequence sw:p ...
... DOTPLOT. Compare the two sequences 1.seq and 2.seq by "dotplot" analysis. Run COMPARE with the output directed to DOTPLOT ( DOTPLOT uses the output from COMPARE to make a 2D plot) Identification of repeats with DOTPLOT. COMPARE may be used to identify repeats in a sequence. Analyze the sequence sw:p ...
Two-hybrid screening
Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay.