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Translation

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Anatomy and Physiology Chapter #4

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Classwork May 15th
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Macromolecules - Van Buren Public Schools
Macromolecules - Van Buren Public Schools

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Bone building: perfect protein

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Solutions to 7.012 Problem Set 1

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Keystone review powerpoint content only with Images

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Connective Tissue - White Plains Public Schools

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... 1. Figure 1 in the article shows a pair of scissors cutting out a gene of interest. What kind of enzyme do the scissors represent? What techniques might you use to confirm that the gene of interest has been inserted into the plasmid (the circular piece of DNA)? 2. Besides the gene of interest, what ...
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Insulin Activity ()

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Genetic threading (Power point)
Genetic threading (Power point)

... • A protein is built up from a chain of amino acids linked by peptide bonds • There are 20 amino acids that can be divided into several classes based on size and other chemical and physical properties • Depending on type of a residue, protein could be either hydrophilic (water loving) or ...
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Two-hybrid screening



Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay.
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