Lab Activity 1
... • Passage of solutes through a semi-permeable membrane. • Pores in the dialysis membrane are of a certain size. • Protein stays in; water, salts, protein fragments, and other molecules smaller than the pore size pass through. ...
... • Passage of solutes through a semi-permeable membrane. • Pores in the dialysis membrane are of a certain size. • Protein stays in; water, salts, protein fragments, and other molecules smaller than the pore size pass through. ...
Ch 2d power point
... Most are globular proteins that act as biological catalysts Some enzymes are pure protein, some have a cofactor, usually a metal ion or an organic molecule derived from vitamins Enzymes are chemically specific ...
... Most are globular proteins that act as biological catalysts Some enzymes are pure protein, some have a cofactor, usually a metal ion or an organic molecule derived from vitamins Enzymes are chemically specific ...
Protein Synthesis
... of DNA known as promoters, which have specific base sequences. Promoters are “signals” in DNA that tell the enzyme where to bind, to start transcription. Similar signals called Repressors tell transcription to stop. ...
... of DNA known as promoters, which have specific base sequences. Promoters are “signals” in DNA that tell the enzyme where to bind, to start transcription. Similar signals called Repressors tell transcription to stop. ...
DNA properties.
... - Hydrophobic effect is mostly responsible for making a compact globule. Final specific tertiary structure is formed by van der Waals interactions, HB, disulfide bonds. - Secret of stability of native structures is not in the magnitude of the interactions but in their cooperativity. ...
... - Hydrophobic effect is mostly responsible for making a compact globule. Final specific tertiary structure is formed by van der Waals interactions, HB, disulfide bonds. - Secret of stability of native structures is not in the magnitude of the interactions but in their cooperativity. ...
Whey Protein Concentrate
... much (anabolism, as in muscle building), but dramatically decreased protein breakdown (catabolism, as in muscle breakdown). Whey is digested more quickly and releases amino acids into the blood stream at much faster rates resulting in a significant increase in protein synthesis (anabolism). However, ...
... much (anabolism, as in muscle building), but dramatically decreased protein breakdown (catabolism, as in muscle breakdown). Whey is digested more quickly and releases amino acids into the blood stream at much faster rates resulting in a significant increase in protein synthesis (anabolism). However, ...
Chapter 3
... • Amino acids are small molecules with a simple basic structure, a carbon atom to which three groups are added: • an amino group (—NH2) • a carboxyl group (—COOH) • a functional group (R) ...
... • Amino acids are small molecules with a simple basic structure, a carbon atom to which three groups are added: • an amino group (—NH2) • a carboxyl group (—COOH) • a functional group (R) ...
Nutrition intervention in sarcopenia
... and still able to accelerate the benefits of exercise, just not to the same extent as milk- or other animal-based proteins. So if someone is vegetarian, I would suggest soy protein as a priority in their diet. But such studies have been conducted in younger subjects; no one has looked at older subje ...
... and still able to accelerate the benefits of exercise, just not to the same extent as milk- or other animal-based proteins. So if someone is vegetarian, I would suggest soy protein as a priority in their diet. But such studies have been conducted in younger subjects; no one has looked at older subje ...
Amino acids - Workforce3One
... the shape of a protein. - caused by changes in the protein’s environment -pH -temperature -salt concentration - causing loss of function. - may involve complete unfolding - Renaturation is refolding into natural shape ...
... the shape of a protein. - caused by changes in the protein’s environment -pH -temperature -salt concentration - causing loss of function. - may involve complete unfolding - Renaturation is refolding into natural shape ...
transcription_and_translation_2
... – then uses one strand of DNA, the template strand, as a template from which nucleotides are assembled to create a strand of RNA. ...
... – then uses one strand of DNA, the template strand, as a template from which nucleotides are assembled to create a strand of RNA. ...
To Fold or Not To Fold
... other to form a a specific protein, they do so through a special kind of covalent bond that is called “peptide bonds”. • In the formation of the bond, water is released. What remains is called a “residue”. Residues are the beads of the ...
... other to form a a specific protein, they do so through a special kind of covalent bond that is called “peptide bonds”. • In the formation of the bond, water is released. What remains is called a “residue”. Residues are the beads of the ...
Prescott`s Microbiology, 9th Edition Chapter 9 –Antimicrobial
... enzymes? Explain. Yes. Since they are chemically related to penicillin and contain the target -lactam ring, they can be degraded by lactamase enzymes. It is important to note however that these drugs are typically resistant to the early lactamase enzymes that cleave penicillin and closely related c ...
... enzymes? Explain. Yes. Since they are chemically related to penicillin and contain the target -lactam ring, they can be degraded by lactamase enzymes. It is important to note however that these drugs are typically resistant to the early lactamase enzymes that cleave penicillin and closely related c ...
Cell Organelles
... its growth phase. New cell membrane is required. Describe the where it is made and its pathway to its destination on the outer cell membrane. ...
... its growth phase. New cell membrane is required. Describe the where it is made and its pathway to its destination on the outer cell membrane. ...
Localization of the P1 protein of potato Y potyvirus in association
... proteinases (Dougherty & Selmer, 1993). Functions or putative roles have been assigned to most of the mature viral proteins although additional activities may be associated with polyprotein intermediates (reviewed by Dougherty & Carrington, 1988 ; Riechmann et al., 1992). However, the role of the Nt ...
... proteinases (Dougherty & Selmer, 1993). Functions or putative roles have been assigned to most of the mature viral proteins although additional activities may be associated with polyprotein intermediates (reviewed by Dougherty & Carrington, 1988 ; Riechmann et al., 1992). However, the role of the Nt ...
SINGAPORE’S R&D FRAMEWORK and the TECHNOLOGY DEVELOPMENT
... Proteins Database: >12,000 3D structures in PDB. Protein diversity: 17% in PDB with unique sequence. Advances in structural genomics: 10,000 unique proteins within 5 years. ...
... Proteins Database: >12,000 3D structures in PDB. Protein diversity: 17% in PDB with unique sequence. Advances in structural genomics: 10,000 unique proteins within 5 years. ...
to find the lecture notes for lecture 4 cellular physiology click here
... • this processing occurs very late in maturation = trans face – processing is catalyzed by protein-specific enzymes called proteases – some proteases are unique to the specific secretory protein – occurs in secretory vesicles that bud from the trans-Golgi face – processing could be at one site (albu ...
... • this processing occurs very late in maturation = trans face – processing is catalyzed by protein-specific enzymes called proteases – some proteases are unique to the specific secretory protein – occurs in secretory vesicles that bud from the trans-Golgi face – processing could be at one site (albu ...
Colorimetric Methods for Determining Protein Concentration. Goals
... 1% solution = 1 g/100 mL = 10 g/L = 10 mg/mL. • to compare with colorimetric data, necessary to have an A(280,0.1%). For BSA, A(280,1%) = 6.6 so A(280,0.1%) = 0.66. • units of A(280,0.1%): (mL/mg)(cm-1). • A(280,0.1%): the absorbance a 0.1% OR 1 mg/mL protein solution would have. ...
... 1% solution = 1 g/100 mL = 10 g/L = 10 mg/mL. • to compare with colorimetric data, necessary to have an A(280,0.1%). For BSA, A(280,1%) = 6.6 so A(280,0.1%) = 0.66. • units of A(280,0.1%): (mL/mg)(cm-1). • A(280,0.1%): the absorbance a 0.1% OR 1 mg/mL protein solution would have. ...
Regulation of Gene Expression
... suggests found in ribosomes which function to synthesise proteins Messenger RNA (mRNA) - This type of RNA specifies the sequence of amino acids in a protein by triplet codon bases. The mRNA sequence is translated into a protein sequence. Transfer RNA (tRNA) - This RNA acts as an intermediate between ...
... suggests found in ribosomes which function to synthesise proteins Messenger RNA (mRNA) - This type of RNA specifies the sequence of amino acids in a protein by triplet codon bases. The mRNA sequence is translated into a protein sequence. Transfer RNA (tRNA) - This RNA acts as an intermediate between ...
X-ray Crystallography
... wavelength used for the diffraction experiment. Atoms in proteins which are suitable for this purpose are sulfur or heavier atoms, for example metal ions in metalloproteins. The most commonly used atom for phase determination via MAD, however, is selenium, since it is usually possible to replace the ...
... wavelength used for the diffraction experiment. Atoms in proteins which are suitable for this purpose are sulfur or heavier atoms, for example metal ions in metalloproteins. The most commonly used atom for phase determination via MAD, however, is selenium, since it is usually possible to replace the ...
Biomolecules
... • Organic molecules contain carbon • Carbon’s four valence electrons allow it to form up to four covalent bonds • Hydrocarbons consist only of C and H – Propane CH8 ...
... • Organic molecules contain carbon • Carbon’s four valence electrons allow it to form up to four covalent bonds • Hydrocarbons consist only of C and H – Propane CH8 ...
PDF - Bentham Open
... NrfD/PsrC protein family. It is involved in the quinolquinone redox system [10]. It is assumed that only DsrP proteins from proteobacterial sulfur-oxidizing bacteria bind heme. The heme b that was found in DsrP could be involved in electron transfer from DsrP to DsrM. The putative quinone binding si ...
... NrfD/PsrC protein family. It is involved in the quinolquinone redox system [10]. It is assumed that only DsrP proteins from proteobacterial sulfur-oxidizing bacteria bind heme. The heme b that was found in DsrP could be involved in electron transfer from DsrP to DsrM. The putative quinone binding si ...
Proteins are made of subunits called amino acids and are
... Circle the AMINO group and draw a square around the CARBOXYL group. ...
... Circle the AMINO group and draw a square around the CARBOXYL group. ...
Carbon compounds class web14
... each of the 4 macromolecules. Put the name of the macromolecule in the first column, the monomers it is composed of in the second, and the function in the cell in the third. ...
... each of the 4 macromolecules. Put the name of the macromolecule in the first column, the monomers it is composed of in the second, and the function in the cell in the third. ...
Two-hybrid screening
Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay.