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Biomolecular chemistry 4. From amino acids to proteins
Biomolecular chemistry 4. From amino acids to proteins

Valea LifeScience09 R
Valea LifeScience09 R

... made up of pharmaceutically uninteresting parts, whereas only a small part of the protein is directly involved in its actual biological activity, for example forming the binding pocket of an enzyme or the epitope of an antigen. Those active parts of a protein often tend to be conserved between prote ...
Identification of two novel mutations associated
Identification of two novel mutations associated

Gene Expression
Gene Expression

... responses to stimuli in the environment to keep you alive. For example, when you are frightened, the DNA tells your adrenal cells to produce adrenaline which will help you respond to the situation. ...
Transcription from DNA Virus Genomes
Transcription from DNA Virus Genomes

... • Nuclear RNAs (hnRNAs) > mRNA • hnRNAs have 5’ caps and 3’ poly A • All Adenoviral L RNAs map to the same promoter • Adeno L mRNAs have 4 parts, 5’ terminal tripartite leader and body ...
Jasmita_presen_nov3
Jasmita_presen_nov3

... In silico screening Structure based virtual screening docking methods to fit putative ligands into 3D structure of target receptor ...
Bioinformatics and Functional Genomics, Chapter 8, Part 1
Bioinformatics and Functional Genomics, Chapter 8, Part 1

... of a protein. Its size is often 10 to 20 amino acids. Simple motifs include transmembrane domains and phosphorylation sites. These do not imply homology when found in a group of proteins. PROSITE (www.expasy.org/prosite) is a dictionary of motifs (there are currently 1600 entries). In PROSITE, a pat ...
Document
Document

... • When mRNA leaves nucleus it has a blueprint of DNA’s instructions. • mRNA goes to ribosomes in cytoplasm • Ribosomes read the blueprint on mRNA. ...
Protein synthesis
Protein synthesis

... polypeptide chains  Many polypeptide chains are covalently modified, either while they are still attached to the ribosome (cotranslational) or after their synthesis has been completed (posttranslational).  These modifications may include removal of part of the translated sequence, or the covalent ...
C8eBookCh05LegendsTables Щ Figure 5.1 Why do scientists study
C8eBookCh05LegendsTables Щ Figure 5.1 Why do scientists study

... amino acid, shown above. Although transthyretin has only one  helix region (see tertiary structure), other globular proteins have multiple stretches of  helix separated by nonhelical regions. Some fibrous proteins, such as -keratin, the structural protein of hair, have the  helix formation over ...
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Healthy or ill: Just a single wrong fold - Reizende DNA-labs
Healthy or ill: Just a single wrong fold - Reizende DNA-labs

... DNA in one cell it would have a length of about 2 meters, though the average cell is only 20 m long. To fit this large string in a cell the DNA is normally folded tightly into chromosomes. But in order to read the DNA proteins have to be able to get near to it. To make this possible there are protei ...
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MCB Test 3 Review

Exam II Name
Exam II Name

... 20. The specific type of bond between the amino acids in a protein is called a ____________ bond. a. hydrophilic b. protease c. peptide d. James 21. The highest quality protein, sometimes referred to as the reference protein, is: a. chicken b. soybeans c. egg white d. milk 22. A child comes to your ...
Describe in simple terms the chemical nature of sugars, proteins
Describe in simple terms the chemical nature of sugars, proteins

... Buffers convert strong acids and bases to weak acids and bases, which keeps the pH in the desired range. There are three buffer systems, the protein buffer system, the carbonic acid-bicarbonate buffer system, and the phosphate buffer system. The carbonic acid-bicarbonate buffer system: ...
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1) (1) If Cedric wanted to purify a native protein that was fusing to a

... 1) (1) If Cedric wanted to purify a native protein that was fusing to a 6xHis tag, how would he purify the protein a) gel-filtration chromatography b) reversed-phase HPLC c) affinity chromatography d) anion-exchange chromatography e) cation-exchange chromatography 2) (1) In comparing the behavior of ...
Name Miss Papassara Sangtanoo Position Research assistant
Name Miss Papassara Sangtanoo Position Research assistant

... and Bangkok Convention Centre Central World, Bangkok, Thailand. ...
Chapter 4 Answers to Even Numbered Study Questions
Chapter 4 Answers to Even Numbered Study Questions

... polysaccharide. In bacteria and archaea that lack a defined cell envelope, it is the polysaccharide portion of the glycolipid of the outer leaf of the cell membrane; hydrogen bonding among these chains prevent swelling of the cell. 4. Pseudomurein and murein are similar in that they both consist of ...
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Cystic Fibrosis

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macromolecules - BHSBiology-Cox

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Basics of BLAST - GEP Community Server

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Gene Section RPS27 (ribosomal protein S27) Atlas of Genetics and Cytogenetics

... binding domain on MDM2. Once bound, MDM2 is stimulated to ubiquinate and degrade the RPS27 or p53, whichever it is bound to. When RPS27 levels are elevated, it can out-compete p53 for MDM2 binding and subsequent degradation, thus stabilizing p53 levels. This would be an appropriate cellular response ...
Moonlighting proteins—an update
Moonlighting proteins—an update

... perform these different functions.11 The helical La motif domain binds to the 3 0 UUU-OH of RNA to protect the RNA from 3 0 -end digestion. Another domain, RRM1, with helices and a beta-sheet, is also needed in a second function as a chaperone to assist in RNA folding. Yeast cytoplasmic peroxiredoxi ...
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GHW#11-Questions$Slides

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Lh6Ch03cProtSeq
Lh6Ch03cProtSeq

... evolution of proteins and their functions (consensus sequences ...
< 1 ... 352 353 354 355 356 357 358 359 360 ... 466 >

Two-hybrid screening



Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay.
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