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Carbohydrates, Lipids, and proteins
Carbohydrates, Lipids, and proteins

... Both of the functional groups are covalently bonded to a central atom, called the alpha carbon Also bonded to the alpha carbon is a hydrogen atom and a chemical group symbolized by the letter R.  R group is the variable part of an amino acid.  R group structure determines the specific properties o ...
101 -- 2006
101 -- 2006

... a) It has high energy phosphate bonds. c) Its hydrolysis is endergonic. b) Its synthesis is exergonic. d) It is extremely stable. e) It is readily obtained from an organism's environment. __ 39. When NAD+ is converted to NADH, it was a) oxidized b) reduced c) phosphorylated d) denatured e) hydrolyze ...
Notes - Part 2.
Notes - Part 2.

... The amino acid composition is much less monotonous than that of silk fibroin. There is an abundance of residues favouring an -helix, such as leucine, alanine and glutamate, and no proline at all. The sequence contains a hierarchy of repeating units. The basic repeat unit is 7 residues long (abcdefg ...
1.5 Page 4 - csfcbiology
1.5 Page 4 - csfcbiology

... The introns must be removed from the RNA transcript before the resulting mRNA can be translated – ribozymes remove the introns and slice the exons together. The mRNA is then moved through the nuclear membrane into the cytoplasm where translation takes place. ...
Biochemistry Quiz
Biochemistry Quiz

... 22. The main difference between the secondary and quaternary structure of a protein is (a) bond angles between amino acids (b) sequence of amino acids (c) number of polypeptides in the molecule (d) the folding pattern of the molecule (e) The disulphide bridges 23. The 'primary structure' of a protei ...
Slide 1
Slide 1

... AMINO ACID METABOLISM • Protein synthesis – Mechanism • Protein synthesis controlled by DNA in the nucleus of cells • DNA is divided into subunits of 3 bases specific for each amino acid • Messenger RNA is produced from DNA • Messenger RNA migrates to ribosomes where it acts as the template for pro ...
Document
Document

... but whose topologies, when deduced from their sequences, strongly suggest that they evolved after Archaea, Bacteria and Eukaryotes had already diversified. In other words, these universal genes appear to be younger than the taxonomic groups in which they are found today “ That is to say, there was a ...
Name: Correctly complete the following statements with a term that
Name: Correctly complete the following statements with a term that

... 28. At a conference, the speaker's grand finale was sautéing mealworms (insect larvae) in butter and serving them to the audience. They were crunchy (like popcorn) because their exoskeletons contain the polysaccharide (a) linolenic acid (b) cellulose (c) collagen (d) glycogen (e) chitin. 29. ______ ...
Amino Acids Interactions
Amino Acids Interactions

... These are all joined to a central α-carbon atom (see following diagram) When used as the building blocks for polypeptide chains (protein) they will be linked to each other through the NH2 and COOH groups (shown later). ...
Biological sequence analysis
Biological sequence analysis

Fall_Final_Exam_Review
Fall_Final_Exam_Review

... transport molecules against the concentration gradient. The protein must change conformation (shape) in order to do this. It requires energy. ...
Macromolecules Reading Activity updated 9-14-11
Macromolecules Reading Activity updated 9-14-11

... structural materials. Carbohydrates are molecules composed of carbon, hydrogen, and oxygen; the ratio of hydrogen atoms to oxygen atoms is 2:1. Simple carbohydrates commonly referred to as sugars, can be monosaccharides if they are composed of single molecules, or disaccharides if they are composed ...
Chlamydomonas reinhardtii strains carrying the stb1-1
Chlamydomonas reinhardtii strains carrying the stb1-1

... Supplemental Fig 5.: Functional complementation of the sta4 mutants. The original mutant strains I73 (sta4-1) and I97 (sta4-2) were transformed with the complete PHOB genomic fragment amplified by PCR and cloned in the pSL18 plasmid (see methods). The paromomycin resistant clones were screened by z ...
PPT CH 18
PPT CH 18

... • Most common type of secondary structure • Coiled, helical • Important features: – Each amide H and carbonyl O is involved in H bonds locking the helix in place – Carbonyl O links to amide H 4 amino acids away – H bonds are parallel to the long axis of the helix – Helix is right-handed – Repeat dis ...
Problem Sets / Exams - Department of Chemistry ::: CALTECH
Problem Sets / Exams - Department of Chemistry ::: CALTECH

... hydrogen-deuterium exchange and mass spectrometry to determine folding intermediates of the protein ribonuclease H. i. (2 points) In the experiment, the authors unfold ribonuclease H in the presence of D2O and then refold the protein in the presence of H2O. If an amino acid residue shows a high D:H ...
lecture08_11
lecture08_11

... • Broaden homology – Detect similar function where sequence differs (only ~50% remote homologies can be detected based on sequence) ...
Human colon tissue lysate - soluble fraction (male, 25 years)
Human colon tissue lysate - soluble fraction (male, 25 years)

... Human colon tissue lysate - soluble fraction (male, 25 years) ab42058 Overview Product name ...
New roles for structure in biology and drug discovery
New roles for structure in biology and drug discovery

... protein structures become available, there will ensue an increase in the rate at which lead molecules for modulating target functions are produced and optimized, ultimately generating an increased flow of drug candidates to the clinic. However, it is not only structures of drug targets that will aid ...
Protein Structure
Protein Structure

... up information easily. Even while being held down, he would struggle mightily, assuming different forms before revealing any of his secrets. Although proteins were not named after Proteus, the description could not be more appropriate. Proteins are complex macromolecules made up of successive amino ...
Powerpoint - Oregon State University
Powerpoint - Oregon State University

PGLO Transformation LAB AP LAB 7
PGLO Transformation LAB AP LAB 7

... http://www.agen.ufl.edu/~owens/age2062/OnLineBiology/OLBB/www.emc.maricopa.edu/faculty/farabee/BIOBK/14_1.jpg ...
Organic Molecules Worksheet: Review
Organic Molecules Worksheet: Review

... Proteins are organic molecules that form muscles, transport O2 (hemoglobin), and act as hormones and enzymes. Most importantly, proteins determine how our bodies look and function. Their building block is the amino acid. Proteins are made of amino acids linked by a peptide bond. When groups of amino ...
Supplemental Information
Supplemental Information

Nucleotide sequence of a cytomegalovirus single
Nucleotide sequence of a cytomegalovirus single

... CMV protein to HSV-1 ICP8 argue that it plays a comparable role in infection, although no genetic evidence for its function(s) is available. This laboratory is studying the CMV ssDNA-binding protein as a model for the structure and function of this group-common gene product. To facilitate these stud ...
Promoter Regions
Promoter Regions

... Transcription Start Site: The beginning of RNA transcription. Downstream of binding sequences. Activator: A protein that binds DNA and stabilizes the binding of transcription factors. Activator Site: The region of DNA an activator binds to. Repressor: A protein that binds DNA and destabilizes the bi ...
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Two-hybrid screening



Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay.
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