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Promoter Regions
Promoter Regions

... Transcription Start Site: The beginning of RNA transcription. Downstream of binding sequences. Activator: A protein that binds DNA and stabilizes the binding of transcription factors. Activator Site: The region of DNA an activator binds to. Repressor: A protein that binds DNA and destabilizes the bi ...
Nucleotide sequence of a cytomegalovirus single
Nucleotide sequence of a cytomegalovirus single

... CMV protein to HSV-1 ICP8 argue that it plays a comparable role in infection, although no genetic evidence for its function(s) is available. This laboratory is studying the CMV ssDNA-binding protein as a model for the structure and function of this group-common gene product. To facilitate these stud ...


... ii) Illustrate your answer with one of the following topics from the course: a) Oxygen delivery, b) altitude adjustment, c) enzyme inhibitors (one specific type), d) metabolic regulation (glycogen or glycolysis), or e) regulation of DNA transcription (2 pt). i) Enzyme is in two forms – relaxed (acti ...
2 Molecular - bloodhounds Incorporated
2 Molecular - bloodhounds Incorporated

... chain on either side of the double bond are either both “up” or both “down,” such that both are on the same side of the molecule. • In trans bonds, the two pieces of the molecule are on opposite sides of the double bond, that is, one “up” and one “down” across from each other. • Naturally-occurring ...
Protein Synthesis
Protein Synthesis

... where protein synthesis occurs. Meanwhile, another type of RNA, transfer RNA, picks up amino acids and transfers them to the site of protein synthesis. There are many different tRNAs, each specific for a particular amino acid. Each tRNA also contains a specific triplet of nucleotides (an anti-codon) ...
Organic Compounds
Organic Compounds

... • Lower the temp, the slower the molecules collide – slower the reaction rate • Higher the temp, the faster the molecules move around – faster the reaction rate • Too high a temperature (60-70 C) – protein denatures – reaction doesn’t occur ...
Control of milk protein synthesis in the mammary gland and the
Control of milk protein synthesis in the mammary gland and the

3 - Moodle NTOU
3 - Moodle NTOU

... • Lipids are the one class of large biological molecules that do not form polymers. • The unifying feature of lipids is having little or no affinity for water. • Lipids are hydrophobic becausethey consist mostly of hydrocarbons, which form nonpolar covalent bonds. • The most biologically important ...
Biology 3 Winter 2009 First Exam
Biology 3 Winter 2009 First Exam

... While all three are triglycerides, they differ in the relative number of carbon to carbon double bonds in their fatty acid tails. Saturated fats have no carbon to carbon double bonds and are unkinked. Monounsaturated fats have a single carbon to carbon double bond in each of their fatty acid tails a ...
RNA Polymerase II Subunit Rpb9 Regulates Transcription
RNA Polymerase II Subunit Rpb9 Regulates Transcription

... experiments, 5⬘-AGAAGATAACACCTTTTTGAG-3⬘ (Dalton Chemicals), is complementary to nucleotides ⫹37 to ⫹17 in the ADH1 gene. The primer was radiolabeled at the 5⬘-end by phosphorylating with polynucleotide kinase (New England Biolabs Inc.) and [␥-32P]ATP. For each primer extension reaction, 15 ␮g of to ...
Glossary of Key Terms in Chapter Two
Glossary of Key Terms in Chapter Two

... code of the mRNA resulting in the substitution of one amino acid in the protein. poly(A) tail (20.4) a tract of 100-200 adenosine monophosphate units covalently attached to the 3’ end of a eukaryotic mRNA molecule. polysome (20.6) complexes of many ribosomes all simultaneously translating a single m ...
Glossary of Key Terms in Chapter Two
Glossary of Key Terms in Chapter Two

... code of the mRNA resulting in the substitution of one amino acid in the protein. poly(A) tail (20.4) a tract of 100-200 adenosine monophosphate units covalently attached to the 3’ end of a eukaryotic mRNA molecule. polysome (20.6) complexes of many ribosomes all simultaneously translating a single m ...
Molecular Biology -
Molecular Biology -

...  amino acid sequence in a polypeptide which folds into a ____________ translation  structure and function of the protein (e.g. normal hemoglobin vs. sickle cell hemoglobin)  person's characteristics or traits (e.g. normal health vs. sickle cell anemia) 2. The double helix structure of DNA, transc ...
Protein Unit Study Guide/Review Sheets
Protein Unit Study Guide/Review Sheets

... Protein Unit Study Guide/Review Sheets You should begin studying now for your test on Thursday! If you have questions, make sure to ask them. Stop in before or after school. Review questions: 1. What elements comprise proteins? C, H, O, N 2. Are proteins organic? YES – CONTAIN CARBON AND HYDROGEN, T ...
three possibile models for replication
three possibile models for replication

... Errors in the DNA, RNA, and Protein Sequence 45. Changes to the DNA sequence (i.e. mutations) are not all harmful…some can be positive (ex: they may increase genetic variability  more possible forms of traits so that not all organisms can be killed off by any one factor like a disease that kills al ...
Biological membranes: the basics and why they are
Biological membranes: the basics and why they are

Phylogenetic analysis of peste des petits ruminants virus (PPRV
Phylogenetic analysis of peste des petits ruminants virus (PPRV

... Turkey, Ivory Coast, and Nigeria, based on the partial nucleotide and amino acid sequences of the gene encoding the F protein of PPRV. As shown in the Table, the partial (F) protein nucleotide sequence of the Iranian PPRV isolate exhibited the highest degree of homology with most of the strains. Non ...
The Carboxyl-Terminal Region of Protein C Is
The Carboxyl-Terminal Region of Protein C Is

... chased for up to 6 hours. None of the truncated variants were efficiently secreted from CHO cells (Fig 2A). The protein C messenger RNA (mRNA) levels of each cell line were comparable (Fig 1B), indicating that impaired secretion of the mutant proteins was not due to the depressed levels of transcrip ...
Document
Document

...  = partial specific volume of the solute (units: cm3/g) One rough estimation of the partial specific volume of a protein, which may be used if the sequence of the protein is not known, is: average partial specific volume of proteins = 0.725 cm^3/g Because the average of experimentally determined p ...
Amino Acids - Building Blocks of Proteins
Amino Acids - Building Blocks of Proteins

... a biological structure and the function it must perform. At the macro level, Darwin recognized that the structure of a finch’s beak was related to the food it ate. This fundamental structure-function relationship is also true at all levels below the Potassium macro level, including proteins and othe ...
In this essay you should have written it as two
In this essay you should have written it as two

... 2 Molecules of ATP are required to start the process Net gain of 2 ATP are produced Diagram can be used to show the above points maximum of 3 Kreb's cycle is an aerobic process / needs oxygen in the cell and occurs in the central liquid matrix of the mitochondrion 3C Pyruvic acid is converted to 2C ...
Lecture 4: bioenergetics and metabolism (mitochondria and
Lecture 4: bioenergetics and metabolism (mitochondria and

swift.cmbi.ru.nl
swift.cmbi.ru.nl

... Important records in SwissProt (3) Features section: post-translational modifications, signal peptides, binding sites, enzyme active sites, domains, disulfide bridges, local secondary structure, sequence conflicts between references etc. etc. ...
The Diversity of Lysine-Acetylated Proteins in Escherichia coli
The Diversity of Lysine-Acetylated Proteins in Escherichia coli

... and highly regulated posttranslational modification. However, it has not been systematically studied in prokaryotes. By affinity immunoseparation using an anti-acetyllysine antibody together with nano-HPLC/MS/MS, we identified 125 lysineacetylated sites in 85 proteins among proteins derived from . T ...
Protein synthesis in the Liver and the Urea Cycle
Protein synthesis in the Liver and the Urea Cycle

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Two-hybrid screening



Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay.
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