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Macromolecules - WordPress.com
Macromolecules - WordPress.com

...  Monounsaturated fats—found in olive, canola, and peanut oils—appear to have the best effect on blood cholesterol, decreasing the level of LDLs and VLDLs and increasing the level of HDLs. Polyunsaturated fats—found in margarine and sunflower, soybean, corn, and safflower oils—are considered more ...
Amino Acid Requirements and Post-absorptive Metabolism in Cattle
Amino Acid Requirements and Post-absorptive Metabolism in Cattle

... given by Swanson (1977): “Furthermore, whichever portion of the fecal N is designated MFN, it is converted to maintenance requirement for protein only when modified by appropriate factors for utilization efficiency of feed proteins”. An efficiency factor was not applied as this would have yielded ma ...
Protein Secondary Structure
Protein Secondary Structure

05_lecture_presentation
05_lecture_presentation

... • Lipids are the one class of large biological molecules that do not form polymers • The unifying feature of lipids is having little or no affinity for water • Lipids are hydrophobic because they consist mostly of hydrocarbons, which form nonpolar covalent bonds • The most biologically important lip ...
protein
protein

... • Sickle-cell disease, an inherited blood disorder, results from a single amino acid substitution in the protein hemoglobin ...
This Exam contains 12 pages and consists of 168 Points.
This Exam contains 12 pages and consists of 168 Points.

... B (3 pts each/9 pts). Listed below are generalizations that apply to biochemical structures or functions. Pick any three of the five. In many, but not all, there are clear exceptions to the rule as it is stated. In those cases, provide a single good example of an exception in the space provided. If ...
Finding Disease Genes
Finding Disease Genes

Structure of the Coat Protein-binding Domain of
Structure of the Coat Protein-binding Domain of

... Electrostatic interactions are somewhat nonspeci®c, especially when they do not involve a single pair of oppositely charged residues. However, this type of interaction, active over long distances, would serve well for the initial recognition and binding events. In order to lock the scaffolding and c ...
James Bond Cellular Spy - UNC
James Bond Cellular Spy - UNC

... The students will be working in groups. Begin this activity by addressing the students as cellular spies; tell them their mission is to secretly crack a DNA code. Each group will approach “M”(the teacher) to receive their specific “mission” or assignment, which is to crack a specific code (see codes ...
What do genes do? - The Open University
What do genes do? - The Open University

... Unless otherwise stated, this resource is released under the terms of the Creative Commons Licence v4.0 http://creativecommons.org/licenses/by-nc-sa/4.0/deed.en_GB. Within that The Open University interprets this licence in the following way: www.open.edu/openlearn/about-openlearn/frequently-asked-q ...
Biochemistry: Monomers and Polymers
Biochemistry: Monomers and Polymers

... • Lipids are nonpolar, uncharged, molecules that include fats, oils, and cholesterol. – Fats and oils are made up of fatty acids bonded together. – Fatty acids are the monomers for lipids. – Fatty acids are made up of carbon chains bonded with oxygen and hydrogen. ...
Novel Food Information - Drought Tolerant Corn - MON 87460
Novel Food Information - Drought Tolerant Corn - MON 87460

... Corn event MON 87460 differs from conventional corn by the insertion of two novel genes; cspb and nptII and their associated regulatory elements. The insertion of these genes results in the expression of two novel proteins in MON87460; CSPB and NPTII. The expression of CSPB confers resistance to dro ...
The N-end rule pathway as a nitric oxide sensor controlling the
The N-end rule pathway as a nitric oxide sensor controlling the

... Mammalian R-transferases are strong sequelogs1 of yeast (fungal) ATE1 R-transferases. However, while the inactivation of mouse ATE1 results in embryonic lethality2, a deletion of S. cerevisiae ATE1 renders cells unable to degrade reporters with N-terminal Asp or Glu but has not been found to cause a ...
cell cycle
cell cycle

AP Biology - Richfield Public Schools
AP Biology - Richfield Public Schools

...  Introduction: Brief statement of purpose, background knowledge of the concepts, and hypothesis. (less the 100 words)  Materials and Procedures: Brief explanation of what you will do and what you will use.  Results/ Data Collection and Analysis: Data Tables, Graph with title, X and Y Labeled.  C ...
book ppt - Castle High School
book ppt - Castle High School

... Missense mutations are substitutions by one amino acid for another in a protein. Example: Sickle-cell disease—allele differs from normal by one base pair Missense mutations may result in a defective protein, reduced protein efficiency, or even a gain of function as in the TP53 gene. ...
Chapter 10 DNA to Protein
Chapter 10 DNA to Protein

... Missense mutations are substitutions by one amino acid for another in a protein. Example: Sickle-cell disease—allele differs from normal by one base pair Missense mutations may result in a defective protein, reduced protein efficiency, or even a gain of function as in the TP53 gene. ...
Optimal Dietary Protein Levels in Juvenile Electric Blue Cichlid
Optimal Dietary Protein Levels in Juvenile Electric Blue Cichlid

... diets for 12 weeks and attained 5.2 to 6.4-fold increases in final weight. Fish regulate feed intake to satisfy their energy requirements (Wilson, 2002). In this feeding trial, fish were fed to visual satiation and achieved maximum growth by controlling feed intake. In general, the feed intake dropp ...
RELIATech GmbH
RELIATech GmbH

... Anti-human LYVE-1 Description: Produced from sera of rabbits immunised with highly pure recombinant human soluble LYVE-1 produced in insect cells. The recombinant soluble LYVE-1consists of amino acid 24 (Ser) to 232 (Gly) and is fused to a C-terminal His-tag (6xHis). LYVE-1 has been identified as a ...
Hormones of a pituitary gland
Hormones of a pituitary gland

... It is secreted continuously during the whole life Secretion is stimulated inhibited by somatostatin ...
C483 Study Guide for Exam 1 Summer 2016 Basic Information
C483 Study Guide for Exam 1 Summer 2016 Basic Information

...  All papers, books, phones, and electronic devices must be in a sealed bag under your seat. Exam Content: The exam will cover chapters 1-6. All material covered in classnotes, book, and homework could be on the exam. Details from case studies will not be included, but problems of that sort are on t ...
New Product Highlights Monoclonal Anti
New Product Highlights Monoclonal Anti

Proteolytic Enzymes
Proteolytic Enzymes

... Protein spots/bands that have been resolved by electrophoresis can be subject to further analysis using Mass Spectrometry (MS). Digestive enzymes such as lysyl endopeptidase and trypsin are used to fragment target proteins within the excised gel sample in preparation for MS analysis. Proteins can be ...
C. Flow Chart
C. Flow Chart

Identification of Antigenic Regions of Duck Hepatitis B Virus Core
Identification of Antigenic Regions of Duck Hepatitis B Virus Core

... plasmid encoding the entire viral genome, indicating that pCI-C plasmid expresses the entire DHBc protein of correct size. No DHBV core gene products were detected in the control cells transfected with empty pCI plasmid. Anti-DHBc humoral response elicited in ducks after DNA or protein immunization ...
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Two-hybrid screening



Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay.
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