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An ATPase domain common to prokaryotic cell cycle proteins, sugar
An ATPase domain common to prokaryotic cell cycle proteins, sugar

... adenylate kinase, recA protein, elongation factor Tu, or ras oncogene protein p21 (8). These three structures are not only similar in threedimensional fold but probably also in some aspects of mechanism: their ATPase active sites are lined with identical or similar residues and the overall domain st ...
Identification of Antigenic Regions of Duck Hepatitis B Virus Core
Identification of Antigenic Regions of Duck Hepatitis B Virus Core

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THE PROTEIN NON-FOLDING PROBLEM: AMINO ACID
THE PROTEIN NON-FOLDING PROBLEM: AMINO ACID

... disordered parts show significantly less sequence similarity than do the ordered parts (work in progress), suggesting that identification of disordered regions by homology is apparently an effective way to increase the information content after all. An additional problem is that a corresponding regi ...
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Bio 102 Practice Problems
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Identification of Upregulated Genes under Cold Stress in Cold

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1 Abstract

DO NOT OPEN UNTIL TOLD TO START
DO NOT OPEN UNTIL TOLD TO START

... A. Differs in sequence from the chimpanzee genome by 1% due to single nucleotide differences. B. Most proteins orthologous to chimpanzee proteins either do not differ in amino acid sequence or differ for just a few amino acids. C. Human genome has more genes related to color perception than the mous ...
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Two-hybrid screening



Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay.
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