Dehydrogenase in Saccharomyces cerevisiae
... derived from the LPDl nucleotide sequence of S. cereuisiae, supports the conclusion that, in this organism, the lipoamide dehydrogenase N-terminal region represents a mitochondrial targeting sequence. The first 20 amino acids are rich in seryl, threonyl and basic residues, and show a complete absenc ...
... derived from the LPDl nucleotide sequence of S. cereuisiae, supports the conclusion that, in this organism, the lipoamide dehydrogenase N-terminal region represents a mitochondrial targeting sequence. The first 20 amino acids are rich in seryl, threonyl and basic residues, and show a complete absenc ...
chapter 3: the cell - CM
... • Enclosed in phospholipid bilayer similar to plasma membrane; termed nuclear envelope • Contains most of cell’s DNA and is primary location for making most RNA • DNA and RNA control more specific organelle functions by coding for and synthesizing proteins ...
... • Enclosed in phospholipid bilayer similar to plasma membrane; termed nuclear envelope • Contains most of cell’s DNA and is primary location for making most RNA • DNA and RNA control more specific organelle functions by coding for and synthesizing proteins ...
Statistical Selection of Amino Acids Fortifying a Minimal Defined
... In addition, the use of minimal defined media might be more cost-effective than using complex rich media due to the lower medium costs with no requirement for the removal of unknown complex compounds during downstream processes [16]. However, complex media based on yeast extract and peptone have bee ...
... In addition, the use of minimal defined media might be more cost-effective than using complex rich media due to the lower medium costs with no requirement for the removal of unknown complex compounds during downstream processes [16]. However, complex media based on yeast extract and peptone have bee ...
Biochemistry I, Spring Term 2003 - Second Exam:
... 5. Which of the statements regarding enzymes is false? a) Enzymes are usually proteins that function as catalysts. b) Enzymes are usually specific. c) Enzymes may be used many times for a specific reaction. d) The active site of an enzyme remains rigid and does not change shape. 6. The nucleophile t ...
... 5. Which of the statements regarding enzymes is false? a) Enzymes are usually proteins that function as catalysts. b) Enzymes are usually specific. c) Enzymes may be used many times for a specific reaction. d) The active site of an enzyme remains rigid and does not change shape. 6. The nucleophile t ...
New concepts of endoplasmic reticulum function in the heart
... folding of nascent ER proteins, which include ischemia, hypertrophy and heart failure, can result in ER stress (A). Under non-stressed conditions, the ER-luminal chaperone, glucose-regulated protein 78 kDa (GRP78) associates with the luminal domains of the 3 proximal effectors of ER stress, PERK (B) ...
... folding of nascent ER proteins, which include ischemia, hypertrophy and heart failure, can result in ER stress (A). Under non-stressed conditions, the ER-luminal chaperone, glucose-regulated protein 78 kDa (GRP78) associates with the luminal domains of the 3 proximal effectors of ER stress, PERK (B) ...
An enlarged largest subunit or Plasmodium falciparum RNA
... The genes encoding the largest subunits of eukaryotic RNA polymerases I (RPI) (2), II (RPII) (3-8), and in (RPITJ) (3) have been isolated and are single copy genes with the exception of Trypanasoma brucei RPII, which contains two alleles (7,8). RPII is a complex enzyme consisting of multiple subunit ...
... The genes encoding the largest subunits of eukaryotic RNA polymerases I (RPI) (2), II (RPII) (3-8), and in (RPITJ) (3) have been isolated and are single copy genes with the exception of Trypanasoma brucei RPII, which contains two alleles (7,8). RPII is a complex enzyme consisting of multiple subunit ...
PDF 28 - The Open University
... bond is known as a double bond. So in this case we have a double bond between each of the oxygen atoms and the carbon atom. ...
... bond is known as a double bond. So in this case we have a double bond between each of the oxygen atoms and the carbon atom. ...
BIOMOLECULES: INTRODUCTION, STRUCTURE AND FUNCTION
... physics were being made virtually everyday, a Dutch scientist Gerardus Mulder described the presence of a substance in living tissues as something which was “without doubt the most important of all substances of the living world, and without it life on our planet would probably not exist”. On sugges ...
... physics were being made virtually everyday, a Dutch scientist Gerardus Mulder described the presence of a substance in living tissues as something which was “without doubt the most important of all substances of the living world, and without it life on our planet would probably not exist”. On sugges ...
Characterization of Acetyl-CoA Carboxylases in the Basal
... Plastid ACC blocking compounds are used as commercially available herbicides, while mitochondrial and cytosolic versions are studied for human illnesses such as diabetes and obesity [11]. Nomenclature of ACCs also varies depending on the organism. For the purposes of our study, we will refer to plas ...
... Plastid ACC blocking compounds are used as commercially available herbicides, while mitochondrial and cytosolic versions are studied for human illnesses such as diabetes and obesity [11]. Nomenclature of ACCs also varies depending on the organism. For the purposes of our study, we will refer to plas ...
Degree of reduction
... storage. In addition, lipids constitute portions of more complex molecules, such as lipopolysaccharides. ...
... storage. In addition, lipids constitute portions of more complex molecules, such as lipopolysaccharides. ...
jcby1101-tutorial2
... Secondary structure: when the sequence of amino acids are linked by hydrogen bonds • α-helix: bonds every 4 aa • β-pleated sheet: parallel polypeptides (note: bonds occur in polypeptide backbone ) Tertiary structure: attractions between helices and sheets • irregular loops and folds that give ov ...
... Secondary structure: when the sequence of amino acids are linked by hydrogen bonds • α-helix: bonds every 4 aa • β-pleated sheet: parallel polypeptides (note: bonds occur in polypeptide backbone ) Tertiary structure: attractions between helices and sheets • irregular loops and folds that give ov ...
Protein Synthesis
... must be read so amino acids can be assembled to make polypeptides (proteins) • This process is called ...
... must be read so amino acids can be assembled to make polypeptides (proteins) • This process is called ...
Gene encoding the group B streptococcal protein R4, its
... product used to screen the 76-043 GBS genomic library. Using the R4 protein-generated probe, two clones were obtained and used for sequencing the r4 gene. The entire open reading frame for the gene encoding R4 was subcloned. The resulting clones were sequenced from the 5’ nucleotide sequences encodi ...
... product used to screen the 76-043 GBS genomic library. Using the R4 protein-generated probe, two clones were obtained and used for sequencing the r4 gene. The entire open reading frame for the gene encoding R4 was subcloned. The resulting clones were sequenced from the 5’ nucleotide sequences encodi ...
Metabolic Pathway Flux Enhancement by Synthetic
... 2.1. Selecting protein–protein interaction domains and ligands for scaffold construction The first decision to be made for scaffolding a metabolic pathway is the choice of colocation components. Each enzyme is translationally fused to a ligand specific for a protein–protein interaction domain. A tra ...
... 2.1. Selecting protein–protein interaction domains and ligands for scaffold construction The first decision to be made for scaffolding a metabolic pathway is the choice of colocation components. Each enzyme is translationally fused to a ligand specific for a protein–protein interaction domain. A tra ...
an introduction to alpha-fetoprotein and the growth inhibitory peptide
... Fig 8 Molecular configurations of human AFP (Left) and human albumin (Right) based on the predicted secondary structures. The amino acid residues participating in the formation of alpharespectively. helices, beta- sheets, beta-turns, and random coils are indicated by The loops formed by disulfide bo ...
... Fig 8 Molecular configurations of human AFP (Left) and human albumin (Right) based on the predicted secondary structures. The amino acid residues participating in the formation of alpharespectively. helices, beta- sheets, beta-turns, and random coils are indicated by The loops formed by disulfide bo ...
Molecule of the Month: AgrA DNA Binding Domain AgrA is the
... contains both a histidine kinase (AgrC) and a response regulator (AgrA) (Figure 1). The other components of the system (AgrB and AgrD) function to generate the active form of Autoinducing Peptid (AIP). AgrD is the precursor to AIP and upon being synthesized binds to AgrB (a transmembrane protein). A ...
... contains both a histidine kinase (AgrC) and a response regulator (AgrA) (Figure 1). The other components of the system (AgrB and AgrD) function to generate the active form of Autoinducing Peptid (AIP). AgrD is the precursor to AIP and upon being synthesized binds to AgrB (a transmembrane protein). A ...
NIH Public Access
... [1-5]. Two S6K1 isoforms (Accession No. NM003161; αI and αII isoforms) are produced from a single gene by alternative mRNA splicing and the use of an alternative translational start site [6]. The 525 residue αI isoform contains an N-terminal 23 residue segment that encodes a polybasic nuclear locali ...
... [1-5]. Two S6K1 isoforms (Accession No. NM003161; αI and αII isoforms) are produced from a single gene by alternative mRNA splicing and the use of an alternative translational start site [6]. The 525 residue αI isoform contains an N-terminal 23 residue segment that encodes a polybasic nuclear locali ...
Document
... Select known 3D structures of lipase (for example). Compare the target sequence with the known lipase structure by magic fit of Swiss-pdb Viewer. Use center the molecule on one atom tool bar to focus on the region of binding site. Compare the above two structures. ...
... Select known 3D structures of lipase (for example). Compare the target sequence with the known lipase structure by magic fit of Swiss-pdb Viewer. Use center the molecule on one atom tool bar to focus on the region of binding site. Compare the above two structures. ...
medical management: portosystemic vascular anomalies (psva)
... creatinine (reflecting muscle mass) and ammonium biurate crystalluria. Initial assessments are done at 7 to 10 day intervals. If all goes well (HE controlled, maintained weight, no ammonium biurates) additional protein supplements may be explored. This is done using 0.3 gm/protein/kg BWt additional ...
... creatinine (reflecting muscle mass) and ammonium biurate crystalluria. Initial assessments are done at 7 to 10 day intervals. If all goes well (HE controlled, maintained weight, no ammonium biurates) additional protein supplements may be explored. This is done using 0.3 gm/protein/kg BWt additional ...
Methods S1.
... mitochondrial respiration was initiated using glutamate (20mM, Aldrich, Steinheim, Germany) with malate (20mM, Aldrich, Steinheim, Germany). Exactly 60 s later, state 3 respiration was initiated by 200 μmol/L ADP injected into the respiration chamber. Respiration rates were recorded under state 3 co ...
... mitochondrial respiration was initiated using glutamate (20mM, Aldrich, Steinheim, Germany) with malate (20mM, Aldrich, Steinheim, Germany). Exactly 60 s later, state 3 respiration was initiated by 200 μmol/L ADP injected into the respiration chamber. Respiration rates were recorded under state 3 co ...
Two-hybrid screening
Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay.