Cancer Prone Disease Neurofibromatosis type 2 (NF2) Atlas of Genetics and Cytogenetics
... Location: 22q12.1-12.2 junction, (incidentally not far from EWS (Ewing tumour)) DNA/RNA Description: 16 exons; 120 kb. Transcription: alternate splicing after exon 15. Protein Protein has been called schwannomin or SCH. Description: 590 or 595 amino acids; 66 kDa; domains: NH2 -- membrane binding -- ...
... Location: 22q12.1-12.2 junction, (incidentally not far from EWS (Ewing tumour)) DNA/RNA Description: 16 exons; 120 kb. Transcription: alternate splicing after exon 15. Protein Protein has been called schwannomin or SCH. Description: 590 or 595 amino acids; 66 kDa; domains: NH2 -- membrane binding -- ...
Determination of protein regions responsible for interactions of
... to identify the possible regions responsible for the amelogenin– LAMP1 interaction. We cloned the amelogenin cDNA fragments corresponding to exons 3 and 5 (amino acids 3–33) or the amino acids corresponding to exon 6D (residues 155–179) into the ‘bait’ vector (pGBKT7). We cloned the human LAMP1 cDNA ...
... to identify the possible regions responsible for the amelogenin– LAMP1 interaction. We cloned the amelogenin cDNA fragments corresponding to exons 3 and 5 (amino acids 3–33) or the amino acids corresponding to exon 6D (residues 155–179) into the ‘bait’ vector (pGBKT7). We cloned the human LAMP1 cDNA ...
Engineering of factors determining alpha-amylase and
... CGTase (mutant) proteins. Plasmid pCT2, a derivative of pUC18 containing the amyA (cgt) gene of T. thermosulfurigenes EM1 (Haeckel and Bahl, 1989; Wind et al., 1995), was used for sitedirected mutagenesis, sequencing and expression of wild-type and mutant CGTase proteins. Plasmid-carrying bacterial ...
... CGTase (mutant) proteins. Plasmid pCT2, a derivative of pUC18 containing the amyA (cgt) gene of T. thermosulfurigenes EM1 (Haeckel and Bahl, 1989; Wind et al., 1995), was used for sitedirected mutagenesis, sequencing and expression of wild-type and mutant CGTase proteins. Plasmid-carrying bacterial ...
BrevdueNord.dk Additional Thoughts on Nutrition for Racing Gordon
... been felt that lactic acidosis was the main cause of such damage. However, studies in humans have indicated that, rather than increased levels of lactic acid as the cause of damage and pain, it is microscopic muscular tears that cause the problem. Whether such tears are the also the cause of muscula ...
... been felt that lactic acidosis was the main cause of such damage. However, studies in humans have indicated that, rather than increased levels of lactic acid as the cause of damage and pain, it is microscopic muscular tears that cause the problem. Whether such tears are the also the cause of muscula ...
Transcription and RNA processing
... expression of adenovirus IVa2 & L4 protein is an example of: ...
... expression of adenovirus IVa2 & L4 protein is an example of: ...
Dicot and monocot plants differ in retinoblastoma
... domains raised the possibility that their function might also be conserved at the molecular level. Furthermore, the order of the conserved domains is similar in all RB proteins identified. The N-termini of RB proteins are highly variable in length and sequence, which may determine the specificity of ...
... domains raised the possibility that their function might also be conserved at the molecular level. Furthermore, the order of the conserved domains is similar in all RB proteins identified. The N-termini of RB proteins are highly variable in length and sequence, which may determine the specificity of ...
Heterogeneity of Genome and Proteome Content in Bacteria
... In prokaryotes, we find a correlation between gene expression levels and the presence of a Shine–Dalgarno (SD) sequence, which plays an important role in translation initiation (see below). In bacterial cells, translation initiation is generally considered the ratelimiting step of translation (review ...
... In prokaryotes, we find a correlation between gene expression levels and the presence of a Shine–Dalgarno (SD) sequence, which plays an important role in translation initiation (see below). In bacterial cells, translation initiation is generally considered the ratelimiting step of translation (review ...
Problem Set Four
... nutrient agar + 200 μg/ml Ampicillin at 37°C. How would you isolate a mutation that would suppress the targeting defect of the SS*Bla mutant? Include what gene might contain the mutation and what your selection or screen would be. Q6. Many E. coli strains resistant to streptomycin (StrR) contain a m ...
... nutrient agar + 200 μg/ml Ampicillin at 37°C. How would you isolate a mutation that would suppress the targeting defect of the SS*Bla mutant? Include what gene might contain the mutation and what your selection or screen would be. Q6. Many E. coli strains resistant to streptomycin (StrR) contain a m ...
maize silage sampling and interpretation of analysis
... Visualise a ‘W’ pattern on the face of the silage clamp. Take at least 9 samples from 15 – 20cm behind the face at different horizontal and vertical positions on this ‘W’. Only sample areas where mould is present if these areas of the clamp will be fed. Preferably samples should be prepared by ‘ ...
... Visualise a ‘W’ pattern on the face of the silage clamp. Take at least 9 samples from 15 – 20cm behind the face at different horizontal and vertical positions on this ‘W’. Only sample areas where mould is present if these areas of the clamp will be fed. Preferably samples should be prepared by ‘ ...
Evolutionary Relationships and Protein Domain Architecture in an
... are modular and have evolved by fusion of a calmodulin-like gene with a protease precursor domain. In addition to typical calpains that conform to the four-domain structure, unconventional calpains have been identified in mammals and in other eukaryotes (Dear et al. 1997; Sorimachi et al. 1997; Margi ...
... are modular and have evolved by fusion of a calmodulin-like gene with a protease precursor domain. In addition to typical calpains that conform to the four-domain structure, unconventional calpains have been identified in mammals and in other eukaryotes (Dear et al. 1997; Sorimachi et al. 1997; Margi ...
The nucleotide sequence of the gene encoding the attachment
... proposed, phocine distemper virus (PDV), responsible for distemper in seals. It is now well established that morbillivirus virions contain six proteins (Rima, 1983): the nucleocapsid (N) protei n, the phosphoprotein (P), the large (L) protein, the matrix protein (M) and two integral membrane protein ...
... proposed, phocine distemper virus (PDV), responsible for distemper in seals. It is now well established that morbillivirus virions contain six proteins (Rima, 1983): the nucleocapsid (N) protei n, the phosphoprotein (P), the large (L) protein, the matrix protein (M) and two integral membrane protein ...
PathogenBioinformatics
... source providing the most current scientific literature on Listeria. Chain Link provides sources for purchasing the strains and microarrays. Click on Path Info. Here you will see information about the organism and associated references in outline format. As you scroll down, you will find a table und ...
... source providing the most current scientific literature on Listeria. Chain Link provides sources for purchasing the strains and microarrays. Click on Path Info. Here you will see information about the organism and associated references in outline format. As you scroll down, you will find a table und ...
Protein-RNA interactions: Structural analysis and functional classes
... structures have been solved. However, the publication of the structure of the 50S and 30S ribosome subunits in 2000,1,2 and the advent of the structural genomics projects means that structural information for more than 350 protein–RNA complexes is currently available. This increased volume of data m ...
... structures have been solved. However, the publication of the structure of the 50S and 30S ribosome subunits in 2000,1,2 and the advent of the structural genomics projects means that structural information for more than 350 protein–RNA complexes is currently available. This increased volume of data m ...
Chapter 5 Macromolecules
... • Lipids are the one class of large biological molecules that do not form polymers • The unifying feature of lipids is having little or no affinity for water • Lipids are hydrophobic because they consist mostly of hydrocarbons, which form nonpolar covalent bonds • The most biologically important lip ...
... • Lipids are the one class of large biological molecules that do not form polymers • The unifying feature of lipids is having little or no affinity for water • Lipids are hydrophobic because they consist mostly of hydrocarbons, which form nonpolar covalent bonds • The most biologically important lip ...
Protein splicing of PRP8 mini-inteins from species of the genus
... Abstract Inteins are protein-intervening sequences found inside the coding region of different host proteins and are translated in-frame with them. They can self-excise through protein splicing, which ligates the host protein flanks with a peptide bond. In this study, four different species of the g ...
... Abstract Inteins are protein-intervening sequences found inside the coding region of different host proteins and are translated in-frame with them. They can self-excise through protein splicing, which ligates the host protein flanks with a peptide bond. In this study, four different species of the g ...
5 end
... put together for each protein? There are 20 amino acids, but there are only four nitrogenous bases in DNA How many bases correspond to an amino acid? Remember – Proteins are strings of amino acid monomers put together ...
... put together for each protein? There are 20 amino acids, but there are only four nitrogenous bases in DNA How many bases correspond to an amino acid? Remember – Proteins are strings of amino acid monomers put together ...
- Journal of Allergy and Clinical Immunology
... should probably be viewed as less commonly allergenic. However, the association of some of these foods with allergic reactions has not been particularly well investigated, and therefore the strength of the clinical information for each source should be considered independently.13 If a gene is obtain ...
... should probably be viewed as less commonly allergenic. However, the association of some of these foods with allergic reactions has not been particularly well investigated, and therefore the strength of the clinical information for each source should be considered independently.13 If a gene is obtain ...
Text S1.
... “For the smaller non-polar residues this number is equal to the maximum area loss that could occur in going from an isolated α-helix to a fully buried environment in the complex. For the larger non-polar residues only a fraction of this value is used since they can never be totally buried in a singl ...
... “For the smaller non-polar residues this number is equal to the maximum area loss that could occur in going from an isolated α-helix to a fully buried environment in the complex. For the larger non-polar residues only a fraction of this value is used since they can never be totally buried in a singl ...
Laboratory #2: Analysis of Cell Survival and Hunting For Mutations
... In general, mutagens fall into two different categories: chemical mutagens and radation. Chemical mutagens that are common in the lab to induce mutations are EMS (ethylmethane sulfonate), and 5 bromo-uracil. Other types of chemical mutagens are Nitrous acid, acridine orange and ethidium bromide, as ...
... In general, mutagens fall into two different categories: chemical mutagens and radation. Chemical mutagens that are common in the lab to induce mutations are EMS (ethylmethane sulfonate), and 5 bromo-uracil. Other types of chemical mutagens are Nitrous acid, acridine orange and ethidium bromide, as ...
Two-hybrid screening
Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay.