Choosing an antibody
... followed by an anti-rabbit IgG secondary antibody. For techniques using samples that do not contain endogenous immunoglobulin (IgG), the choice of host species of the primary antibody is less critical. An example is western blotting of a cell lysate that is not expected to contain IgG. However, tiss ...
... followed by an anti-rabbit IgG secondary antibody. For techniques using samples that do not contain endogenous immunoglobulin (IgG), the choice of host species of the primary antibody is less critical. An example is western blotting of a cell lysate that is not expected to contain IgG. However, tiss ...
Ribozymes
... Hammerhead and hairpin ribozymes can be found in several satellite RNAs associated with RNA plant viruses (e.g. tobacco ringspot virus) X ...
... Hammerhead and hairpin ribozymes can be found in several satellite RNAs associated with RNA plant viruses (e.g. tobacco ringspot virus) X ...
Lecture_14.pps
... • N-glycosylation occurs in ER-Topology for lysosomal degradation is wrong • ~50% of ER proteins misfold and are degraded what happens to the sugar chain? To glycopeptides? • Protein synthetic rate and glycosylation rate must be coordinated • Competition for lectin-based chaperones ...
... • N-glycosylation occurs in ER-Topology for lysosomal degradation is wrong • ~50% of ER proteins misfold and are degraded what happens to the sugar chain? To glycopeptides? • Protein synthetic rate and glycosylation rate must be coordinated • Competition for lectin-based chaperones ...
Nutritional Impact on Protein Metabolism of Muscle and
... In addition to covalent bonds, which bind amino acids to each other, proteins possess weaker but very important bonds that hold the macromolecule in a unique configuration. Such bonds are quite sensitive to environmental conditions – eg. excessive stirring of a protein solution in air, exposure to u ...
... In addition to covalent bonds, which bind amino acids to each other, proteins possess weaker but very important bonds that hold the macromolecule in a unique configuration. Such bonds are quite sensitive to environmental conditions – eg. excessive stirring of a protein solution in air, exposure to u ...
Critical care: Meeting protein requirements without overfeeding energy
... were similar between local (FeedCalc) guidelines for 'mild' catabolism, 1.2 g protein per Kg (ASPEN lower limit) or adjusted weight (Fig. 1). Local estimates for 'moderate' or 'severe' catabolism, ESPEN and ASPEN upper estimates (2 g/kg/d) or for obese and morbidly obese patients (2 and 2.5 g/kg Ham ...
... were similar between local (FeedCalc) guidelines for 'mild' catabolism, 1.2 g protein per Kg (ASPEN lower limit) or adjusted weight (Fig. 1). Local estimates for 'moderate' or 'severe' catabolism, ESPEN and ASPEN upper estimates (2 g/kg/d) or for obese and morbidly obese patients (2 and 2.5 g/kg Ham ...
Felis domesticus papillomavirus, isolated from a skin lesion, is
... 1994), among others. The FdPV NCR-1 contains a variety of putative regulatory elements, although the FdPV NCR-1 does not include a TATA box within the E6\E7 promoter region. However, multiple SP-1 binding sites were identified that could serve as the transcription initiation site (Peterson et al., 1 ...
... 1994), among others. The FdPV NCR-1 contains a variety of putative regulatory elements, although the FdPV NCR-1 does not include a TATA box within the E6\E7 promoter region. However, multiple SP-1 binding sites were identified that could serve as the transcription initiation site (Peterson et al., 1 ...
AMINO ACIDS I. Function of amino acids A. Building blocks of
... a. Hydroxyproline and hydroxylysine i. hydroxylated enzymatically after translation ii. important in collagen structure b. phosphoamino acids i. Tyr, Ser and Thr hydroxyl groups can be phosphorylated ii. important in activation and inhibition of enzymatic activity c. Cysteine can form disulfide bond ...
... a. Hydroxyproline and hydroxylysine i. hydroxylated enzymatically after translation ii. important in collagen structure b. phosphoamino acids i. Tyr, Ser and Thr hydroxyl groups can be phosphorylated ii. important in activation and inhibition of enzymatic activity c. Cysteine can form disulfide bond ...
Document
... • Lots of information available per gene product • Genetically tractable: • haploid • DNA transformation • multiple genetic markers available, both selection and counterselection possible • genetic crosses possible • gene knockout by homologous recombination very efficient – complete set of 4 x 6000 ...
... • Lots of information available per gene product • Genetically tractable: • haploid • DNA transformation • multiple genetic markers available, both selection and counterselection possible • genetic crosses possible • gene knockout by homologous recombination very efficient – complete set of 4 x 6000 ...
Institute for Plant Physiology and Microbiology - diss.fu
... Koag M-C., Fenton R. D., Wilken S. and Close T. J. (2003) The binding of maize DHN1 to lipid vesicles. Gain of structure and lipid specificity. Plant. Physiol. 131:309316. Laemmli UK. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4 Nature 227:105-132 Lang V ...
... Koag M-C., Fenton R. D., Wilken S. and Close T. J. (2003) The binding of maize DHN1 to lipid vesicles. Gain of structure and lipid specificity. Plant. Physiol. 131:309316. Laemmli UK. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4 Nature 227:105-132 Lang V ...
Chapter 9 – Catalytic Strategies (So we`ve talked about enzymes
... (But by and large Proteases are enzymes that degrade proteins by cleaving peptide bonds … like collagen in jello for example! There are a ton of these in our bodies, but why are they so abundant?) (Mainly for digestion but it also allows us to get rid of proteins that are no longer needed or are dam ...
... (But by and large Proteases are enzymes that degrade proteins by cleaving peptide bonds … like collagen in jello for example! There are a ton of these in our bodies, but why are they so abundant?) (Mainly for digestion but it also allows us to get rid of proteins that are no longer needed or are dam ...
Poon, Andy: Predicting Phosphorylation: A critique of the NetPhos program and potential alternatives
... function or localization. The presence of a phosphate group often modulates that protein's interaction with other proteins upstream or downstream of it on a certain pathway, such as the binding of phosphorylated receptors by proteins with SH2 domains (reviewed by Pawson, 2004). Given the prevalence ...
... function or localization. The presence of a phosphate group often modulates that protein's interaction with other proteins upstream or downstream of it on a certain pathway, such as the binding of phosphorylated receptors by proteins with SH2 domains (reviewed by Pawson, 2004). Given the prevalence ...
blast
... • VecScreen – for detecting cloning vector contamination in sequenced data. see last weeks handout for rest of them ...
... • VecScreen – for detecting cloning vector contamination in sequenced data. see last weeks handout for rest of them ...
Caenorhabditis elegans unc-60 gene encodes
... 1986; Prasad and Baillie 1989). Restriction fragments from F53E2 were used to probe Southern blots containing D N A from C. elegans and the closely related Caenorhabditis briqgsae. To our surprise, most of the probes hybridized at low stringency to a family of C. eleqans and C. briqgsae fragments. B ...
... 1986; Prasad and Baillie 1989). Restriction fragments from F53E2 were used to probe Southern blots containing D N A from C. elegans and the closely related Caenorhabditis briqgsae. To our surprise, most of the probes hybridized at low stringency to a family of C. eleqans and C. briqgsae fragments. B ...
Hydrogen Bond
... Although interaction between ionized (formally charged) groups can be strong they do not contribute significantly to stabilization of protein structure (with exception of some salt bridges or metal coordination sites) Dipole-dipole and VdW interactions are weak and contribute moderately to stabili ...
... Although interaction between ionized (formally charged) groups can be strong they do not contribute significantly to stabilization of protein structure (with exception of some salt bridges or metal coordination sites) Dipole-dipole and VdW interactions are weak and contribute moderately to stabili ...
What are enzymes and how do they work
... 4. What is the next codon that will be read by the ribosome in the schematic above? ___GAA______ 5. What two features of a tRNA allow it to function as an “adapter” molecule between mRNA and protein? 1. contains an anticodon that recognizes the codon 2. carries an amino acid 6. What would happen if ...
... 4. What is the next codon that will be read by the ribosome in the schematic above? ___GAA______ 5. What two features of a tRNA allow it to function as an “adapter” molecule between mRNA and protein? 1. contains an anticodon that recognizes the codon 2. carries an amino acid 6. What would happen if ...
Both DRIs and RDAs refer to long-term average daily nutrient intake
... 25 grams for women 38 grams for men (~ 40 grams /day) Food with low glycemic index tends to • create sense of satiety over a longer period of time& may help to limit caloric intake Carbohydrates are not essential nutrients (T RDA for Carbohydrate is set at 130 g/day • Adults should consume 45 – 65 % ...
... 25 grams for women 38 grams for men (~ 40 grams /day) Food with low glycemic index tends to • create sense of satiety over a longer period of time& may help to limit caloric intake Carbohydrates are not essential nutrients (T RDA for Carbohydrate is set at 130 g/day • Adults should consume 45 – 65 % ...
carbonyl carbons
... • How are they made? – Thermodynamic strategy • Peptide bond formation is coupled to the breakdown of a high energy phosphate bond in a protein factory known as the ribosome. ...
... • How are they made? – Thermodynamic strategy • Peptide bond formation is coupled to the breakdown of a high energy phosphate bond in a protein factory known as the ribosome. ...
IEX and RP Method Development for the Separation of
... Ion Exchange Chromatography of Proteins o Ion-exchange chromatography (IEC) discriminates between proteins on the basis of accessible surface charges and their corresponding electrostatic interaction with the column’s stationary phase. o The degree of protein retention is dependent on the strength ...
... Ion Exchange Chromatography of Proteins o Ion-exchange chromatography (IEC) discriminates between proteins on the basis of accessible surface charges and their corresponding electrostatic interaction with the column’s stationary phase. o The degree of protein retention is dependent on the strength ...
Protein Synthesis Project 1516
... In a process called transcription, which takes place in the nucleus of the cell, messenger RNA (mRNA) reads and copies the DNA’s nucleotide sequences in the form of a complementary RNA molecule. Then ...
... In a process called transcription, which takes place in the nucleus of the cell, messenger RNA (mRNA) reads and copies the DNA’s nucleotide sequences in the form of a complementary RNA molecule. Then ...
Test 1
... How could you tell? Both peptides have a tryptophan. The tryptophan gives them a UV absorbance, so all you have to do is to look at the absorbance of the solution around 280 nm. 6C. (10 points)Suggest a second chromatographic method you could use to separate the above peptides from the protein cytoc ...
... How could you tell? Both peptides have a tryptophan. The tryptophan gives them a UV absorbance, so all you have to do is to look at the absorbance of the solution around 280 nm. 6C. (10 points)Suggest a second chromatographic method you could use to separate the above peptides from the protein cytoc ...
Protein damage on Cu2+
... MCO reactions during IMAC: Presence of hydroxyl radicals – An MCO reaction which includes the Fenton Reaction gives rise to oxidizing species such as OH radical in the reaction between the metal and the peroxide. – The presence of OH radicals indicates that a Fenton reaction (or some other radical g ...
... MCO reactions during IMAC: Presence of hydroxyl radicals – An MCO reaction which includes the Fenton Reaction gives rise to oxidizing species such as OH radical in the reaction between the metal and the peroxide. – The presence of OH radicals indicates that a Fenton reaction (or some other radical g ...
How flexible is α-actinin`s rod domain?
... Abstract: α−actinin, an actin binding protein, plays a Endo, and Ebashi (1967)]. On the other hand, in nonkey role in cell migration, cross-links actin filaments in muscle cells, α-actinin organizes the cortical cytoskelethe Z-disk, and is a major component of contractile mus- ton adjacent to membra ...
... Abstract: α−actinin, an actin binding protein, plays a Endo, and Ebashi (1967)]. On the other hand, in nonkey role in cell migration, cross-links actin filaments in muscle cells, α-actinin organizes the cortical cytoskelethe Z-disk, and is a major component of contractile mus- ton adjacent to membra ...
Document
... • 3’ poly A tail – plays a role in the export of the mRNA into the cytoplasm – after transcription – an enzyme adds 20 to 250 adenine nucleotides after the poly-adenylation signal sequence – also prevents degradation of the mRNA once its in the cytoplasm ...
... • 3’ poly A tail – plays a role in the export of the mRNA into the cytoplasm – after transcription – an enzyme adds 20 to 250 adenine nucleotides after the poly-adenylation signal sequence – also prevents degradation of the mRNA once its in the cytoplasm ...
Two-hybrid screening
Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay.