Biological Chemistry II: Problem Set 1
... The conformation of poly-L-lysine is pH dependent. At relatively high pH values, the polymer adopts a predominantly helical structure, but at acidic and neutral pH it exists as a random coil. Furthermore, at high temperatures the helix converts to an aggregated antiparallel β sheet. Explain these ob ...
... The conformation of poly-L-lysine is pH dependent. At relatively high pH values, the polymer adopts a predominantly helical structure, but at acidic and neutral pH it exists as a random coil. Furthermore, at high temperatures the helix converts to an aggregated antiparallel β sheet. Explain these ob ...
Document
... Surrounded by double membrane and contain own DNA, but codes for very few proteins! (a few dozen) Instead, most genes from prokaryotic ancestor have been transferred to the nucleus, so proteins must be imported ...
... Surrounded by double membrane and contain own DNA, but codes for very few proteins! (a few dozen) Instead, most genes from prokaryotic ancestor have been transferred to the nucleus, so proteins must be imported ...
(Ms,Hu,Dr) from Mouse (Clone: 9E10) - unconj. (IMQ
... Purified IgG supplied in Phosphate buffered saline (PBS) containing 0.09% Sodium azide ...
... Purified IgG supplied in Phosphate buffered saline (PBS) containing 0.09% Sodium azide ...
mtCLIC/CLIC4 a Chloride Channel Protein Participates in Apoptosis
... absent in vitro, these preparations also contained abundant post-apoptotic cells in a more advanced state of degeneration, with completely degraded nuclei, ruptured membranes and deteriorating organelles. In apoptotic cells, however, the mitochondria appeared largely unchanged. To localize mtCLIC, w ...
... absent in vitro, these preparations also contained abundant post-apoptotic cells in a more advanced state of degeneration, with completely degraded nuclei, ruptured membranes and deteriorating organelles. In apoptotic cells, however, the mitochondria appeared largely unchanged. To localize mtCLIC, w ...
Chapter 1 I am - Mrs Smith`s Biology
... I am the class of protein formed by several spiral-shaped polypeptide molecules becoming linked together in parallel by cross-bridges, giving the protein a rope-like structure Amino Acids ...
... I am the class of protein formed by several spiral-shaped polypeptide molecules becoming linked together in parallel by cross-bridges, giving the protein a rope-like structure Amino Acids ...
Hot Topics in Protein Medicinal Chemistry
... David Tirrell, California Institute of Technology “Non-Canonical Amino Acids as Tools for Protein Medicinal Chemistry” ...
... David Tirrell, California Institute of Technology “Non-Canonical Amino Acids as Tools for Protein Medicinal Chemistry” ...
Study guide for research assistants
... DNA to be cloned is cut with restriction enzymes and then ligated into a vector cut with the same restriction enzymes. LIC does not require restriction enzymes or DNA ligase. Note the two major plasmids used, BG1861 and AVA0421, and how they add different histidinebased tags to the target protein. A ...
... DNA to be cloned is cut with restriction enzymes and then ligated into a vector cut with the same restriction enzymes. LIC does not require restriction enzymes or DNA ligase. Note the two major plasmids used, BG1861 and AVA0421, and how they add different histidinebased tags to the target protein. A ...
Beyond Antibodies
... NATURE REVIEWS | DRUG DISCOVERY, VOL 5 | FEB 2006 | pp- 147-159 Hari Hariharan, D.V.M., Ph.DAntibody Therapeutics Meeting December 9, 2010 ...
... NATURE REVIEWS | DRUG DISCOVERY, VOL 5 | FEB 2006 | pp- 147-159 Hari Hariharan, D.V.M., Ph.DAntibody Therapeutics Meeting December 9, 2010 ...
Section 7-3 Skeleton Notes
... Integral Proteins exposed to the Cell's External environment often have carbohydrates attached to them serve as identification badges that allow cells to recognize each other and may act as Site where viruses or chemical messengers such as hormones can attach. 1. Briefly describe the main functions ...
... Integral Proteins exposed to the Cell's External environment often have carbohydrates attached to them serve as identification badges that allow cells to recognize each other and may act as Site where viruses or chemical messengers such as hormones can attach. 1. Briefly describe the main functions ...
Catalysis - University of California, Davis
... (LysNH2, Arg-guanidino) groups which are positively charged at neutral pH. These are generally surface groups. At some pH, the isoelectric point or pI, negative and positive charges balance, and the protein has a net charge of zero. Most globular proteins have pI<6.5, and are polyanions at neutral p ...
... (LysNH2, Arg-guanidino) groups which are positively charged at neutral pH. These are generally surface groups. At some pH, the isoelectric point or pI, negative and positive charges balance, and the protein has a net charge of zero. Most globular proteins have pI<6.5, and are polyanions at neutral p ...
Poly-acrylamide Gel Electrophoresis (PAGE) PAGE is based upon
... Native PAGE • a rarely used technique, although it can be informative. • proteins are not denatured as in SDSPAGE. • one can perform enzymatic assays on bands in gel as we shall do in this class. • “primarily” separates based on mass of proteins, assuming low pI. • is possible to get some idea of su ...
... Native PAGE • a rarely used technique, although it can be informative. • proteins are not denatured as in SDSPAGE. • one can perform enzymatic assays on bands in gel as we shall do in this class. • “primarily” separates based on mass of proteins, assuming low pI. • is possible to get some idea of su ...
Computational (Structural) Biology
... Which proteins (biomolecules) interact with which proteins (biomolecules)? ...
... Which proteins (biomolecules) interact with which proteins (biomolecules)? ...
Document
... (polar) - Tails: Lipids (non-polar) 2. Cholesterol Function- Prevents fatty acid tails from sticking together Location- between fatty acid tails ...
... (polar) - Tails: Lipids (non-polar) 2. Cholesterol Function- Prevents fatty acid tails from sticking together Location- between fatty acid tails ...
notes for mondays lab
... 1. Phosphate Buffered Saline (PBS): a salty solution of constant pH to keep tissues, cells, and proteins intact during maceration 2. Proteinase K: an enzyme that catalyzes the breakdown of cellular proteins by splitting them into smaller peptides and amino acids 3. Buffer AL: a cell lysis solution t ...
... 1. Phosphate Buffered Saline (PBS): a salty solution of constant pH to keep tissues, cells, and proteins intact during maceration 2. Proteinase K: an enzyme that catalyzes the breakdown of cellular proteins by splitting them into smaller peptides and amino acids 3. Buffer AL: a cell lysis solution t ...
Membranes
... • fluid mosaic model – i.e. membrane is not static • both proteins and lipids have considerable freedom of movement: mainly lateral Even with an electron microscope it is not possible to see he molecular structure of a cell membrane. Thus it is necessary to construct a model to explain its various p ...
... • fluid mosaic model – i.e. membrane is not static • both proteins and lipids have considerable freedom of movement: mainly lateral Even with an electron microscope it is not possible to see he molecular structure of a cell membrane. Thus it is necessary to construct a model to explain its various p ...
Immunochemical methods
... Immunohistochemistry or IHC refers to the process of localizing proteins in cells of a tissue section Can be used to answer questions like: • In what cells of a particular tissue is a protein expressed? • Where in that cell is the protein localized? Such information is important to elucidate the fun ...
... Immunohistochemistry or IHC refers to the process of localizing proteins in cells of a tissue section Can be used to answer questions like: • In what cells of a particular tissue is a protein expressed? • Where in that cell is the protein localized? Such information is important to elucidate the fun ...
Book Reviews - Cancer Research
... the proteins play in biology. The fourteenth volume of this excellent series main tains the high standard of quality established by its predecessors. In the foreword it is mentioned that it was anticipated that there would be some duplication of the sixth ...
... the proteins play in biology. The fourteenth volume of this excellent series main tains the high standard of quality established by its predecessors. In the foreword it is mentioned that it was anticipated that there would be some duplication of the sixth ...
Using Computers to teach Undergraduates about Biological Molecules
... the value of a specific algorithm using an extended number of proteins of known structure. Traditionally, molecular modelling has relied upon powerful workstations or expensive minicomputers. However, a large number of accessible programs, for example Alchemy, Moby, HyperChem, are now available for ...
... the value of a specific algorithm using an extended number of proteins of known structure. Traditionally, molecular modelling has relied upon powerful workstations or expensive minicomputers. However, a large number of accessible programs, for example Alchemy, Moby, HyperChem, are now available for ...
D. E. Shaw Research is seeking postdoctoral fellows of exceptional... Postdoctoral Fellowships at D. E. Shaw Research
... a stimulating, rewarding, and flexible work environment, and we are prepared to offer above-market compensation to candidates of truly exceptional ability. To submit an application, please use the link provided below: http://www.deshawresearch.com/recruit/jobs/Oncampus/Saarland/PF D. E. Shaw Researc ...
... a stimulating, rewarding, and flexible work environment, and we are prepared to offer above-market compensation to candidates of truly exceptional ability. To submit an application, please use the link provided below: http://www.deshawresearch.com/recruit/jobs/Oncampus/Saarland/PF D. E. Shaw Researc ...
A genetically programmable protein module as
... Viral Proteases Enzymes that catalyze the hydrolysis of peptide bonds Very specific and highly expressed early on during infection Cleave polyproteins into functional enzymes necessary for infection and disease progression ...
... Viral Proteases Enzymes that catalyze the hydrolysis of peptide bonds Very specific and highly expressed early on during infection Cleave polyproteins into functional enzymes necessary for infection and disease progression ...
Protein Origami
... makes fireflies glow, and a lot more. Proteins also aid viruses in invading cells. AIDS can be tied to proteins that break through cellular defenses and replicate the HIV virus. Cancer is linked to damage in proteins that inhibit uncontrolled cell growth. Alzheimer’s disease is believed to be relate ...
... makes fireflies glow, and a lot more. Proteins also aid viruses in invading cells. AIDS can be tied to proteins that break through cellular defenses and replicate the HIV virus. Cancer is linked to damage in proteins that inhibit uncontrolled cell growth. Alzheimer’s disease is believed to be relate ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.