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Product Data Sheet IMQ-84589_2015.09-v1 c-Myc (9E 10) Mouse Monoclonal Antibody Catalog Number: IMQ-84589 Size: 0.1 ml (1 mg/ml) Class: Monoclonal Type: Antibody Clone: 9E 10 Host / Isotype: Mouse / IgG1 Immunogen: Synthetic peptide (AEEQKLISEEDLLRKRREQLKHKLEQLRNSCA) corresponding to amino acids residues 408-432 of Human c-Myc Myeloma / fusion partners: Cells from immunized Balb/c mice were fused with the Sp2/0-Ag.14 myeloma cell line Species Reactivity: Mouse, Human, Drosophila melanogaster (fruit fly) Specificity: This antibody recognises c-Myc, a proto-oncogenic transcription factor that plays a role in cell proliferation, apoptosis and the development of human tumours. The antibody 9E10 recognises recombinant proteins incorporating a cMyc epitope tag. 9E10 also detects human c-Myc protein and a peptide in random coil configuration not as a helix. Purification: Purified on protein A from tissue culture supernatant. Format: Purified IgG supplied in Phosphate buffered saline (PBS) containing 0.09% Sodium azide Applications: ELISA, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot Dilutions: Optimal antibody dilution should be determined by titration Storage: Store at 4°C for one month, or in small aliquots at -20°C for longer periods. Avoid repeated freezing and thawing as this may denature the antibody. Do not store antibodies in frost-free freezers. For long-term storage aliquote all or part of the unit in volumes not less than 20 µl. In small aliquots antibodies tend to adsorb to the sides of the vials. Before aliquoting predilution of 1:2 - 1:20 with a solution containing other proteins such as 5% BSA is recommendable. References: Evan GL, et al. Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product. Mol Cell Biol. 1985.5(12):3610-6. PMID:3915782 Also known as: avian myelocytomatosis viral oncogene homolog; BHLHE39; c-Myc; Class E basic helix-loop-helix protein 39; MRTL; MYC; Myc proto-oncogene protein; myc-related translation/localization regulatory factor; Proto-oncogene c-Myc; Transcription factor p64; v-myc avian myelocytomatosis viral oncogene homolog; v-myc myelocytomatosis viral oncogene homolog (avian) dianova GmbH ● Warburgstrasse 45 ● 20354 Hamburg, Germany Phone: +49 (0)40 45 06 70 ● Email: [email protected]● www.dianova.com Page 1/2 Product Data Sheet IMQ-84589_2015.09-v1 c-Myc (9E 10) Mouse Monoclonal Antibody Safety Information: This product is not licensed or approved for administration to humans or to animals other than the experimental animals. Standard Laboratory Practices should be followed when handling this material. This reagent contains 0.09% sodium azide (a poisonous and hazardous substance) as preservative. Although this concentration is not regarded as dangerous to health, appropriate measures should be taken to avoid skin and eye contact, inhalation, and ingestion. This product is for In Vitro experimental use only. Not for Therapeutic or Diagnostic use. This product ("Product") is warranted to operate or perform substantially in conformance with published Product specifications in effect at the time of sale, as set forth in the Product documentation, specifications and/or accompanying package inserts ("Documentation") and to be free from defects in material and workmanship. Unless otherwise expressly authorized in writing, Products are supplied for research use only. No claim of suitability for use in applications regulated by FDA is made. The warranty provided herein is valid only when used by properly trained individuals. Unless otherwise stated in the Documentation, this warranty is limited to one year from date of shipment when the Product is subjected to normal, proper and intended usage. This warranty does not extend to anyone other than the original purchaser of the Product ("Buyer"). No other warranties, express or implied, are granted, including without limitation, implied warranties of merchantability, fitness for any particular purpose, or non infringement. Buyer's exclusive remedy for nonconforming Products during the warranty period is limited to replacement of or refund for the non-conforming Product(s). There is no obligation to replace Products as the result of (i) accident, disaster or event of force majeure, (ii) misuse, fault or negligence of or by Buyer, (iii) use of the Products in a manner for which they were not designed, or (iv) improper storage and handling of the Products. For Research Use Only Temperature Limitation Manufacturer Catalog Number Expiry Consult Instructions for Use Lot Number dianova GmbH ● Warburgstrasse 45 ● 20354 Hamburg, Germany Phone: +49 (0)40 45 06 70 ● Email: [email protected]● www.dianova.com Page 2/2