Anti-UGT2A1 antibody ab126527 Product datasheet 1 Image Overview
... Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol ...
... Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol ...
Gene Ontology (GO)
... Saccharomyces Genome Database (SGD) - database for the budding yeast Saccharomyces cerevisiae Mouse Genome Database (MGD) & Gene Expression Database (GXD) - databases for the mouse Mus musculus The Arabidopsis Information Resource (TAIR) - database for the brassica family plant Arabidopsis thaliana ...
... Saccharomyces Genome Database (SGD) - database for the budding yeast Saccharomyces cerevisiae Mouse Genome Database (MGD) & Gene Expression Database (GXD) - databases for the mouse Mus musculus The Arabidopsis Information Resource (TAIR) - database for the brassica family plant Arabidopsis thaliana ...
Slide 1
... Acids, Bases, and Salts Acid A solute that adds hydrogen ions to a solution Strong acids dissociate completely in solution ...
... Acids, Bases, and Salts Acid A solute that adds hydrogen ions to a solution Strong acids dissociate completely in solution ...
introduction
... No: use single sequence methods No: single sequence methods Motif search Secondary structure prediction Use other data ...
... No: use single sequence methods No: single sequence methods Motif search Secondary structure prediction Use other data ...
Exam #1
... Draw the dehydration reaction between two amino acids. First draw each amino acid and indicate the carboxyl group and the amine group on one of them. Indicate the variable part of each amino acid with R1 for the first amino acid, and R2 for the second. Then draw the resulting dipeptide and indicate ...
... Draw the dehydration reaction between two amino acids. First draw each amino acid and indicate the carboxyl group and the amine group on one of them. Indicate the variable part of each amino acid with R1 for the first amino acid, and R2 for the second. Then draw the resulting dipeptide and indicate ...
Alkaline Southern Blotting Kit with 11 × 14 cm BioBond™ Plus
... packages. Additionally, the kit components have been optimized to provide consistent efficient transfer in the shortest possible time. Each complete kit includes all of the solutions for the pretreatment and transfer of deoxyribonucleic acid from agarose gels as well as precut BioBond™ nylon membran ...
... packages. Additionally, the kit components have been optimized to provide consistent efficient transfer in the shortest possible time. Each complete kit includes all of the solutions for the pretreatment and transfer of deoxyribonucleic acid from agarose gels as well as precut BioBond™ nylon membran ...
Functions of proteins
... The “heme group” : - contains an iron atom, and is where the oxygen binds to hemoglobin. - It is an example of a “prosthetic group” or a “cofactor”: a non-amino acid part of a protein. - Proteins with prosthetic groups are called “conjugated proteins”. ...
... The “heme group” : - contains an iron atom, and is where the oxygen binds to hemoglobin. - It is an example of a “prosthetic group” or a “cofactor”: a non-amino acid part of a protein. - Proteins with prosthetic groups are called “conjugated proteins”. ...
Parallel analysis of translated ORF (PLATO)
... -Efficient transfer of DNA fragment: recombination sequence and clonase enzymes (BP and LR reaction) -Maintain the reading frame: specific sites >Allow functional analysis -Low background rates -No requirements on the sequence to be cloned -Large libraries of Gateway-adapted ORFs have been created b ...
... -Efficient transfer of DNA fragment: recombination sequence and clonase enzymes (BP and LR reaction) -Maintain the reading frame: specific sites >Allow functional analysis -Low background rates -No requirements on the sequence to be cloned -Large libraries of Gateway-adapted ORFs have been created b ...
Diapositive 1
... a composite model of βδ/γζ-COP bound to membrane via two molecules of Arf1-GTP. The γζ-COP/Arf1 crystal structure is colored as is the second molecule of Arf1. The remainder of βδ/γζ-COP, in grey. The Nterminal amphipathic α helices of Arf1 (colored red) are modeled in their expected locations as me ...
... a composite model of βδ/γζ-COP bound to membrane via two molecules of Arf1-GTP. The γζ-COP/Arf1 crystal structure is colored as is the second molecule of Arf1. The remainder of βδ/γζ-COP, in grey. The Nterminal amphipathic α helices of Arf1 (colored red) are modeled in their expected locations as me ...
AMIDO BLACK PROTEIN ASSAY
... transfers of destain until the background is nearly white. Examine the intensities of the black spots. Compare the spots from your test protein with those of the standards to obtain an estimate of your protein concentration. If a more accurate estimate is required: Carefully punch out one of each du ...
... transfers of destain until the background is nearly white. Examine the intensities of the black spots. Compare the spots from your test protein with those of the standards to obtain an estimate of your protein concentration. If a more accurate estimate is required: Carefully punch out one of each du ...
CAP5510 - Bioinformatics - Department of Computer and
... • Bioinformatics is the field of science in which biology, computer science, and information technology merge into a single discipline. The ultimate goal of the field is to enable the discovery of new biological insights as well as to create a global perspective from which unifying principles in bio ...
... • Bioinformatics is the field of science in which biology, computer science, and information technology merge into a single discipline. The ultimate goal of the field is to enable the discovery of new biological insights as well as to create a global perspective from which unifying principles in bio ...
Protein Estimation
... 2)IMPORTANCE OF LOWRY’S METHOD Various methods are also used Biuret Method BCA (Bicinchoninic acid assay ) ...
... 2)IMPORTANCE OF LOWRY’S METHOD Various methods are also used Biuret Method BCA (Bicinchoninic acid assay ) ...
Recombinant Human IL-35-Fc fusion protein (ELISA Std.)
... >98%, as determined by Coomassie stained SDS-PAGE. ...
... >98%, as determined by Coomassie stained SDS-PAGE. ...
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Anti-PCB antibody [3H2AD9] ab110314 Product datasheet 3 Images Overview
... PCB in Human fibroblast cells (4% paraformaldehyde fixed and 0.1% Triton X100 permeabilized) followed by Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (red). Note: The target protein locates to the mitochondrial matrix. Lower image shows cells co-stained with an ant ...
... PCB in Human fibroblast cells (4% paraformaldehyde fixed and 0.1% Triton X100 permeabilized) followed by Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (red). Note: The target protein locates to the mitochondrial matrix. Lower image shows cells co-stained with an ant ...
Principles of Skeletal Muscle Adaptation
... – also repress gene for fast II b myosin HC, turn on fast IIa myosin HC – not only enlarged, but change in contractile phenotype – larger, slower contracting fiber. ...
... – also repress gene for fast II b myosin HC, turn on fast IIa myosin HC – not only enlarged, but change in contractile phenotype – larger, slower contracting fiber. ...
Media - Inside Cancer
... fate of this protein? In other words, once it is packaged by the Golgi, what will it do and how will it do it? This growth factor is synthesized by the cell in response to the signal that originally interacted with the cell receptor. Once packaged by the Golgi, the protein will be transported to the ...
... fate of this protein? In other words, once it is packaged by the Golgi, what will it do and how will it do it? This growth factor is synthesized by the cell in response to the signal that originally interacted with the cell receptor. Once packaged by the Golgi, the protein will be transported to the ...
EE 400: Practice using NCBI, Blast and Clustal
... Now that you have collected several proteins that are all similar, we are going to use a multiple alignment program called Clustal to see just how similar they are to each other overall. A. Go to the website: http://www.ch.embnet.org/software/ClustalW.html B. You will see a big box for “Input sequen ...
... Now that you have collected several proteins that are all similar, we are going to use a multiple alignment program called Clustal to see just how similar they are to each other overall. A. Go to the website: http://www.ch.embnet.org/software/ClustalW.html B. You will see a big box for “Input sequen ...
2. Basic Immunologic Procedures
... molecule (venom toxin), consequently antibodies have the ability to cross link many antigen molecules simultaneously. This cross-linking causes the antibody antigen-complex to become insoluble and precipitate out from the solution. The immunoelectrophoresis technique makes use of this capability of ...
... molecule (venom toxin), consequently antibodies have the ability to cross link many antigen molecules simultaneously. This cross-linking causes the antibody antigen-complex to become insoluble and precipitate out from the solution. The immunoelectrophoresis technique makes use of this capability of ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.