Apoptosis of Lung Epithelial Cells in Response to Meconium and
... determined with Bio-Rad protein assay kit (Bio-Rad, Hercules, CA) using the Bradford method. Equal amounts of purified protein from each sample (10 mg) were mixed with 2x gel loading buffer (4% SDS, 20% Glycerol, 120 mM Tris-HCl, pH 6.8, 0.01% bromophenol blue, 2% b-mercaptoethanol), denatured at 95 ...
... determined with Bio-Rad protein assay kit (Bio-Rad, Hercules, CA) using the Bradford method. Equal amounts of purified protein from each sample (10 mg) were mixed with 2x gel loading buffer (4% SDS, 20% Glycerol, 120 mM Tris-HCl, pH 6.8, 0.01% bromophenol blue, 2% b-mercaptoethanol), denatured at 95 ...
Protein Structure Prediction not a trivial matter
... Complex interactions exist between intramolecular atoms and between the protein and the surrounding environment. Number of interactions to track increases exponentially with molecule size The number of possible structures that proteins may possess is extremely large ...
... Complex interactions exist between intramolecular atoms and between the protein and the surrounding environment. Number of interactions to track increases exponentially with molecule size The number of possible structures that proteins may possess is extremely large ...
a sample task
... What is most remarkable to consider is that a hemoglobin molecule is made up of two alpha chains and two beta chains, each consisting of about 150 amino acids, for a total of 600 amino acids in the whole protein. The difference between a normal hemoglobin molecule and a sickle cell molecule—which dr ...
... What is most remarkable to consider is that a hemoglobin molecule is made up of two alpha chains and two beta chains, each consisting of about 150 amino acids, for a total of 600 amino acids in the whole protein. The difference between a normal hemoglobin molecule and a sickle cell molecule—which dr ...
Preparation and transformation of competent bacteria: Calcium
... 41. List any ligands, cofactors or metal ions included in the structure (particularly important for enzymes): Project specific questions. These can be answered using the PDB specific information available at RCSB, or by going to the journal article on which this structure is based (direct link to th ...
... 41. List any ligands, cofactors or metal ions included in the structure (particularly important for enzymes): Project specific questions. These can be answered using the PDB specific information available at RCSB, or by going to the journal article on which this structure is based (direct link to th ...
Modified Green Fluorescence Protein
... when exposed to blue light. The GFP from A. victoria has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm which is in the lower green portion of the visible spectrum. In cell and molecular biology, the GFP gene is frequently used as a report ...
... when exposed to blue light. The GFP from A. victoria has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm which is in the lower green portion of the visible spectrum. In cell and molecular biology, the GFP gene is frequently used as a report ...
Document
... You have identified a novel cytoplasmic protein. Sequence analysis revealed the presence of a number of potential serine/threonine amino acids that are potential phosphorylation sites. Based on this data, you hypothesize that this protein may be a substrate for a A. protein kinase. B. receptor tyros ...
... You have identified a novel cytoplasmic protein. Sequence analysis revealed the presence of a number of potential serine/threonine amino acids that are potential phosphorylation sites. Based on this data, you hypothesize that this protein may be a substrate for a A. protein kinase. B. receptor tyros ...
Representation and Manipulation of 3D Molecular Structures
... • Data mining for conformational properties and intermolecular interactions (CSD & PDB) • Data mining for information about intermolecular interactions (CSD & PDB) • Further understanding of the nature of protein structure and its relationship to amino acid sequence (PDB) • Homology modeling (compar ...
... • Data mining for conformational properties and intermolecular interactions (CSD & PDB) • Data mining for information about intermolecular interactions (CSD & PDB) • Further understanding of the nature of protein structure and its relationship to amino acid sequence (PDB) • Homology modeling (compar ...
23. ______ layers of ______ make up the cell
... metabolic reactions in organisms. Amino acids contain two functional groups, the carboxyl group (-COOH) and the amino group (-NH2). Enzymes are protein molecules that act as biological catalysts. Cells contain thousands of different enzymes to control the functions of the cell. Enzymes must physical ...
... metabolic reactions in organisms. Amino acids contain two functional groups, the carboxyl group (-COOH) and the amino group (-NH2). Enzymes are protein molecules that act as biological catalysts. Cells contain thousands of different enzymes to control the functions of the cell. Enzymes must physical ...
Chapter 6 Proteins and Amino Acids I Introduction II The Structure of
... II The Structure of Amino Acids and of Protein A. What is the structure of an amino acid? 1. central carbon and one hydrogen 2. an acid group (carbon, a oxygen and an OH) (COOH) 3. an amino group (NH2) 4. a side chain, which is different for each amino acid (make amino acids differ in size, shape, a ...
... II The Structure of Amino Acids and of Protein A. What is the structure of an amino acid? 1. central carbon and one hydrogen 2. an acid group (carbon, a oxygen and an OH) (COOH) 3. an amino group (NH2) 4. a side chain, which is different for each amino acid (make amino acids differ in size, shape, a ...
Datasheet PDF - BioAssay Systems
... most important macromolecules in life science. Proteins are polypeptides made up of amino acids and play various key roles in all aspects of biology. Protein quantitation is a very common practice for life scientists. Simple, direct and automation-ready procedures for measuring protein concentration ...
... most important macromolecules in life science. Proteins are polypeptides made up of amino acids and play various key roles in all aspects of biology. Protein quantitation is a very common practice for life scientists. Simple, direct and automation-ready procedures for measuring protein concentration ...
kaedah-kaedah dalam biologi molekul
... TECHNIQUES IN MOLECULAR BIOLOGY • CENTRIFUGATION- Separation of molecules/macromolecules/organelles according to the size, shape, density & gradient • ELECTROPHORESIS- Separation of molecules/macromolecules according to charge • MICROSCOPY- Structural examination of minute molecule/macromolecule/org ...
... TECHNIQUES IN MOLECULAR BIOLOGY • CENTRIFUGATION- Separation of molecules/macromolecules/organelles according to the size, shape, density & gradient • ELECTROPHORESIS- Separation of molecules/macromolecules according to charge • MICROSCOPY- Structural examination of minute molecule/macromolecule/org ...
BMMB597E_lecture3
... • Base this on structure rather than sequence • Structural similarities are maintained as divergence proceeds, better than sequence similarities • For closely related proteins, expect no difference between sequence-based and structure based classification • How far can classification be extended? ...
... • Base this on structure rather than sequence • Structural similarities are maintained as divergence proceeds, better than sequence similarities • For closely related proteins, expect no difference between sequence-based and structure based classification • How far can classification be extended? ...
SUBUNITS FROM REDUCED .AND S
... Examination of the reduced and carboxymethylated protein in 8M ureaacrylamide gels revealed that the protein migrated as one band at high loadings of protein [Fig. 3(d)], but at lower protein concentrations there was evidence of splitting (Fig. 4). A band which is not stained intensely by amido blac ...
... Examination of the reduced and carboxymethylated protein in 8M ureaacrylamide gels revealed that the protein migrated as one band at high loadings of protein [Fig. 3(d)], but at lower protein concentrations there was evidence of splitting (Fig. 4). A band which is not stained intensely by amido blac ...
Bio301 Biochemistry I
... At high ionic strengths the solubilities ofproteins as well as those of most other substances, decrease. This effect is known as salting out. You have given 1.0 M solutions of NaCl, (NH4) 2SO4 and K3PO4.In which of these solutions would you expect that a protein be most soluble? Least soluble? ...
... At high ionic strengths the solubilities ofproteins as well as those of most other substances, decrease. This effect is known as salting out. You have given 1.0 M solutions of NaCl, (NH4) 2SO4 and K3PO4.In which of these solutions would you expect that a protein be most soluble? Least soluble? ...
Whittier Union High School District
... 43. What are the three main stages of cellular respiration (in order)? 1) Glycolysis- takes place in the cytoplasm and produces a total of 4 ATP molecules, but requires 2 ATP to work 2) Kreb’s cycle (AKA citric acid cycle)- takes place in the matrix of the mitochondria and produces 2 ATP molecules 3 ...
... 43. What are the three main stages of cellular respiration (in order)? 1) Glycolysis- takes place in the cytoplasm and produces a total of 4 ATP molecules, but requires 2 ATP to work 2) Kreb’s cycle (AKA citric acid cycle)- takes place in the matrix of the mitochondria and produces 2 ATP molecules 3 ...
Exam III - chem.uwec.edu
... All of these structures form in response to the same driving forces. Non-polar regions are looking for ways to minimize their exposure to water while at the same time the polar, hydrophilic regions with to remain in contact with water. This is called the hydrophobic effect. In the formation of lipid ...
... All of these structures form in response to the same driving forces. Non-polar regions are looking for ways to minimize their exposure to water while at the same time the polar, hydrophilic regions with to remain in contact with water. This is called the hydrophobic effect. In the formation of lipid ...
Nutrition: Protein
... Assuming the athlete is consuming enough calories, this level of protein intake should be sufficient to build muscle when combined with appropriate training. ...
... Assuming the athlete is consuming enough calories, this level of protein intake should be sufficient to build muscle when combined with appropriate training. ...
Helicase-Primase Inhibitors as Novel Anti-HSV
... Paul W. Smith, G/axo WeI/come Research and Development, Hertfordshire, UK Influenza is one of the best-known and most-common diseases, but an effective therapy has still to be found. Therapeutic drugs have included both Amantadine and Rimantidine, which are active only against influenza A virus (the ...
... Paul W. Smith, G/axo WeI/come Research and Development, Hertfordshire, UK Influenza is one of the best-known and most-common diseases, but an effective therapy has still to be found. Therapeutic drugs have included both Amantadine and Rimantidine, which are active only against influenza A virus (the ...
Introduction: Proteins are one of the three major classes of biological
... (R153S), analyzed its activity, and obtained the following results: 1) Adding altered R153S SiR enzyme does not restore growth to bacteria that lack their own SiR when they are grown on media lacking S-2. 2) That same mutant enzyme (R153S) is active for electron transfer in the test ...
... (R153S), analyzed its activity, and obtained the following results: 1) Adding altered R153S SiR enzyme does not restore growth to bacteria that lack their own SiR when they are grown on media lacking S-2. 2) That same mutant enzyme (R153S) is active for electron transfer in the test ...
Chapter 6 Proteins and Amino Acids I Introduction II The Structure of
... VII Using Amino Acids The Fate of an Amino Acid that was originally part of a protein in ...
... VII Using Amino Acids The Fate of an Amino Acid that was originally part of a protein in ...
The exocyst, an octameric protein complex conserved among all
... specialization during the evolution – including involvement in autophagy. Using yeast twohybrid assay it is shown here that Exo70B1 and Exo70B2, but not other Arabidopsis Exo70 paralogs interact with Atg8, an autophagosomal marker. The proximity of these two paralogs and Atg8 in vivo was confirmed b ...
... specialization during the evolution – including involvement in autophagy. Using yeast twohybrid assay it is shown here that Exo70B1 and Exo70B2, but not other Arabidopsis Exo70 paralogs interact with Atg8, an autophagosomal marker. The proximity of these two paralogs and Atg8 in vivo was confirmed b ...
Proteins
... • Membrane proteins have more hydrophobic residues, whereas fibrous proteins may have atypical sequences • Homologous proteins from different organisms have homologous sequences • e.g., cytochrome c is highly conserved ...
... • Membrane proteins have more hydrophobic residues, whereas fibrous proteins may have atypical sequences • Homologous proteins from different organisms have homologous sequences • e.g., cytochrome c is highly conserved ...
Genomics in Drug Discovery
... • PGT method: in some cases too many orthologous relationships, especially for trypsin (73 in mouse and 62 in rat!) • BBH method seems to be more usable for this study, but still not gives an explanation for the differences in CCK levels • Our problem (different CCK responses in Human, Mouse and Rat ...
... • PGT method: in some cases too many orthologous relationships, especially for trypsin (73 in mouse and 62 in rat!) • BBH method seems to be more usable for this study, but still not gives an explanation for the differences in CCK levels • Our problem (different CCK responses in Human, Mouse and Rat ...
Biochemistry (Macromolecules)
... Proteins (A. K.A. Polypeptides) and Enzymes (Enzymes are a TYPE of protein.) A. These macromolecules make up greater than 50% of an organisms dry weight, called biomass. B. Names usually end with the suffix “lin” (i.e. insulin) for proteins and “ase” for enzymes (i.e. sucrase) C. The monomer “buildi ...
... Proteins (A. K.A. Polypeptides) and Enzymes (Enzymes are a TYPE of protein.) A. These macromolecules make up greater than 50% of an organisms dry weight, called biomass. B. Names usually end with the suffix “lin” (i.e. insulin) for proteins and “ase” for enzymes (i.e. sucrase) C. The monomer “buildi ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.