No Slide Title
... • Group of residues with high contact density, number of contacts within domains is higher than the number of contacts between domains. • A stable unit of protein structure that can fold autonomously • A rigid body linked to other domains by flexible linkers • A portion of the protein that can be ac ...
... • Group of residues with high contact density, number of contacts within domains is higher than the number of contacts between domains. • A stable unit of protein structure that can fold autonomously • A rigid body linked to other domains by flexible linkers • A portion of the protein that can be ac ...
Moving Proteins into Membranes and Organelles Moving Proteins
... BiP: a chaperone that prevents nascent chain from misfolding or forming aggregates. PDI: stabilizes proteins with disulfide bonds. ...
... BiP: a chaperone that prevents nascent chain from misfolding or forming aggregates. PDI: stabilizes proteins with disulfide bonds. ...
Core Concept Cheat Sheet
... monosaccharide units linked by glycosidic bonds. ! Protein: A macromolecule composed of one or more polypeptide chains, each with a characteristic sequence of amino acids linked by peptide bonds. ! Amino acids: Alpha amino-substituted carboxylic acids, the building blocks of proteins. ! Primary stru ...
... monosaccharide units linked by glycosidic bonds. ! Protein: A macromolecule composed of one or more polypeptide chains, each with a characteristic sequence of amino acids linked by peptide bonds. ! Amino acids: Alpha amino-substituted carboxylic acids, the building blocks of proteins. ! Primary stru ...
Protein and its functional properties in food
... contain carbon, hydrogen, oxygen and nitrogen. A few also contain sulphur and phosphorous There are around 20 different amino acids commonly found in plant and animal proteins. All amino acids have an acid group (X) and an amino group (Y). The rest of the amino acid is represented by ‘R’ and is diff ...
... contain carbon, hydrogen, oxygen and nitrogen. A few also contain sulphur and phosphorous There are around 20 different amino acids commonly found in plant and animal proteins. All amino acids have an acid group (X) and an amino group (Y). The rest of the amino acid is represented by ‘R’ and is diff ...
Metabolism of amino acids, porphyrins
... • NO relaxes blood vessels, lowers blood pressure, and is a neurotransmitter in the brain • Nitroglycerin is converted to NO and dilates coronary arteries in treating angina pectoris ...
... • NO relaxes blood vessels, lowers blood pressure, and is a neurotransmitter in the brain • Nitroglycerin is converted to NO and dilates coronary arteries in treating angina pectoris ...
Protein Structure - George Mason University
... Why not stick to these methods? • X-ray Diffraction – – Only a small number of proteins can be made to form crystals. – A crystal is not the protein’s native environment. – Very time consuming. • NMR Distance Measurement – – Not all proteins are found in solution. – This method generally looks at i ...
... Why not stick to these methods? • X-ray Diffraction – – Only a small number of proteins can be made to form crystals. – A crystal is not the protein’s native environment. – Very time consuming. • NMR Distance Measurement – – Not all proteins are found in solution. – This method generally looks at i ...
Bacterial Systems for Assembly, Secretion and Targeted
... Pathogenic bacteria of animals and plants have developed an astonishing set of tools that allow them to survive in their hosts. These effectors of infection or virulence factors are often delivered into eukaryotic host cells where they interfere with host cell signalling, thereby causing a variety o ...
... Pathogenic bacteria of animals and plants have developed an astonishing set of tools that allow them to survive in their hosts. These effectors of infection or virulence factors are often delivered into eukaryotic host cells where they interfere with host cell signalling, thereby causing a variety o ...
Chemistry Review
... 3. Tertiary Structure – occurs when the secondary structure folds up in a very specific shape and forms a specific protein. The tertiary structure comes from interactions/bonds between the R groups of the amino acid. Some of the interactions that occur between amino acid R groups are: ...
... 3. Tertiary Structure – occurs when the secondary structure folds up in a very specific shape and forms a specific protein. The tertiary structure comes from interactions/bonds between the R groups of the amino acid. Some of the interactions that occur between amino acid R groups are: ...
Application of recombinant DNA technology in protein expression
... Dense particles, containing precipitated proteins. Their formation depends on protein synthesis rate, growth conditions. Advantages: proteolysis resistant, big yield, relatively pure, easy to separate. Disadvantages: inactive product requires in vitro refolding and renaturation ...
... Dense particles, containing precipitated proteins. Their formation depends on protein synthesis rate, growth conditions. Advantages: proteolysis resistant, big yield, relatively pure, easy to separate. Disadvantages: inactive product requires in vitro refolding and renaturation ...
Chapter 7: Proteins
... Many are primarily protein All body cells break down Protein is needed for continual repair and rebuilding ...
... Many are primarily protein All body cells break down Protein is needed for continual repair and rebuilding ...
Gene Section CPEB4 (cytoplasmic polyadenylation element binding protein 4)
... the 3'UTR of some mRNAs. They were originally described in Xenopus laevis oocytes, where they control translation of maternal mRNAs during meiosis by regulation of the length of the polyA tail (Hake and Richter, 1994). However, these proteins can be also found in other non-germ cells suggesting othe ...
... the 3'UTR of some mRNAs. They were originally described in Xenopus laevis oocytes, where they control translation of maternal mRNAs during meiosis by regulation of the length of the polyA tail (Hake and Richter, 1994). However, these proteins can be also found in other non-germ cells suggesting othe ...
Power Point Presentation
... The use of a hard vacuum allows highly reactive intermediate structures to be used, e.g., a variety of radicals with one or more dangling bonds. Because the intermediates are in a vacuum, and because their position is controlled (as opposed to solutions, where the position and orientation of a molec ...
... The use of a hard vacuum allows highly reactive intermediate structures to be used, e.g., a variety of radicals with one or more dangling bonds. Because the intermediates are in a vacuum, and because their position is controlled (as opposed to solutions, where the position and orientation of a molec ...
protein
... (They are the same 3 as Transcription.) – Initiation - This is building the factory needed to make the protein. • The small sub-unit attaches to the 5’ cap. This interaction signals the large sub unit. • AUG (the start codon on the mRNA molecule) brings in the tRNA (using the anticodon) molecule wit ...
... (They are the same 3 as Transcription.) – Initiation - This is building the factory needed to make the protein. • The small sub-unit attaches to the 5’ cap. This interaction signals the large sub unit. • AUG (the start codon on the mRNA molecule) brings in the tRNA (using the anticodon) molecule wit ...
Phospho-regulation of human Protein Kinase Aurora-A
... Fig. S3. Specificity of the phospho-Thr288 Aurora-A-specific monoclonal antibody pA7.2. Three independent lines of evidence testified to the specificity of the monoclonal phospho-specific antibody pA7.2. Firstly, the antibody selectively recognized the phosphorylated peptide in ELISA screening assay ...
... Fig. S3. Specificity of the phospho-Thr288 Aurora-A-specific monoclonal antibody pA7.2. Three independent lines of evidence testified to the specificity of the monoclonal phospho-specific antibody pA7.2. Firstly, the antibody selectively recognized the phosphorylated peptide in ELISA screening assay ...
Transcriptome and Proteome Analysis Of Arum Lily
... INTRODUCTION. Z. aethiopica is an evergreen perennial plant from Araceae botanical family worldwide known for ornamental purposes being also utilized for African traditional medicine including as antimicrobial source. Nevertheless, as others non-model plants, this species has no abundant molecular a ...
... INTRODUCTION. Z. aethiopica is an evergreen perennial plant from Araceae botanical family worldwide known for ornamental purposes being also utilized for African traditional medicine including as antimicrobial source. Nevertheless, as others non-model plants, this species has no abundant molecular a ...
12 Complement system BA
... C1 is always present in serum but it can operate only on an activating surface Low affinity binding to the Fc region of antibody conformational change activation Multiple interaction with immune complexes ...
... C1 is always present in serum but it can operate only on an activating surface Low affinity binding to the Fc region of antibody conformational change activation Multiple interaction with immune complexes ...
Biological Molecules
... which it can function • Each enzyme has an optimal pH in which it can function • Tertiary structure can be radically altered by changes in pH ...
... which it can function • Each enzyme has an optimal pH in which it can function • Tertiary structure can be radically altered by changes in pH ...
The in vitro catalysis of protein folding by endoplasmic reticulum
... prolyl peptide bonds (4). Isomerisations of Xaa-Pro peptide bonds have been identified as slow steps in in v i m folding of some proteins ( 5 ) . Nascent proteins are presumably all fruns polypeptide chains and cis Xaa-Pro peptide bonds are common under native conditions (6); this suggests a role fo ...
... prolyl peptide bonds (4). Isomerisations of Xaa-Pro peptide bonds have been identified as slow steps in in v i m folding of some proteins ( 5 ) . Nascent proteins are presumably all fruns polypeptide chains and cis Xaa-Pro peptide bonds are common under native conditions (6); this suggests a role fo ...
Topic 4: BIOLOGICALLY IMPORTANT ORGANIC MOLECULES
... (4) function as energy storage materials, insulation and cushioning agent; accumulated in specialized cells called adipocytes Phospholipids- consist of glycerol, esters of two fatty acids plus some highly charged molecule or group ( in the case of Fig. 5.12 it is choline and phosphate). One part of ...
... (4) function as energy storage materials, insulation and cushioning agent; accumulated in specialized cells called adipocytes Phospholipids- consist of glycerol, esters of two fatty acids plus some highly charged molecule or group ( in the case of Fig. 5.12 it is choline and phosphate). One part of ...
Topic 4 - FSU Biology
... (4) function as energy storage materials, insulation and cushioning agent; accumulated in specialized cells called adipocytes Phospholipids- consist of glycerol, esters of two fatty acids plus some highly charged molecule or group ( in the case of Fig. 5.12 it is choline and phosphate). One part of ...
... (4) function as energy storage materials, insulation and cushioning agent; accumulated in specialized cells called adipocytes Phospholipids- consist of glycerol, esters of two fatty acids plus some highly charged molecule or group ( in the case of Fig. 5.12 it is choline and phosphate). One part of ...
Review on Biochemistry: Protein Chemistry
... Anemia: reflect impaired synthesis of Hb (e.g. iron deficiency) or impaired production of erythrocyte (e.g. in folic acid or Vit B12 deficiency). Thalassemias: result from the partial or total absence of one or more or chains of hemoglobin. Apart from marrow transplantation, treatment is sym ...
... Anemia: reflect impaired synthesis of Hb (e.g. iron deficiency) or impaired production of erythrocyte (e.g. in folic acid or Vit B12 deficiency). Thalassemias: result from the partial or total absence of one or more or chains of hemoglobin. Apart from marrow transplantation, treatment is sym ...
Translocation of effector proteins from the oomycete Phytophthora
... translocates effector proteins into host plant cells during infection. Whereas bacteria possess the well characterized type III secretion system, the mechanism used by eukaryotic plant pathogens for delivering effector proteins into the host cell remains unclear. In oomycetes this process depends on ...
... translocates effector proteins into host plant cells during infection. Whereas bacteria possess the well characterized type III secretion system, the mechanism used by eukaryotic plant pathogens for delivering effector proteins into the host cell remains unclear. In oomycetes this process depends on ...
supplementary figures
... the mRNA was purified, reverse transcribed and then qPCR was performed. The expression levels of MMP1 were normalised to GAPDH. qPCR was performed in triplicate for each cDNA template, error bars indicate means +/− SEM, and asterisks significance (p < 0.05; t-test). d. Comparative expression of MMP1 ...
... the mRNA was purified, reverse transcribed and then qPCR was performed. The expression levels of MMP1 were normalised to GAPDH. qPCR was performed in triplicate for each cDNA template, error bars indicate means +/− SEM, and asterisks significance (p < 0.05; t-test). d. Comparative expression of MMP1 ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.