FR in detergent-insoluble complexes - Journal of Cell Science
... In re-immunoprecipitation experiments, after the kinase reaction, samples were boiled for 5 minutes in Hepes buffer with 2% SDS, then diluted 1:20 in the same buffer without SDS. A second round of immunoprecipitation was carried out for 3 hours at 4°C, with antibodies bound to magnetic beads, as bef ...
... In re-immunoprecipitation experiments, after the kinase reaction, samples were boiled for 5 minutes in Hepes buffer with 2% SDS, then diluted 1:20 in the same buffer without SDS. A second round of immunoprecipitation was carried out for 3 hours at 4°C, with antibodies bound to magnetic beads, as bef ...
Protein Function Follows Form: Small Changes may Cause Big
... the chemical properties (polarity, size, charge) of their side chains. • This formative assessment will assure that students understand these properties before the next activities. ...
... the chemical properties (polarity, size, charge) of their side chains. • This formative assessment will assure that students understand these properties before the next activities. ...
A Comparative 2-Dimensional Gel Protein Database of the Intact
... Tsonis, Panagiotis A., "A Comparative 2-Dimensional Gel Protein Database of the Intact and Regenerating Newt Limbs" (1993). Biology Faculty Publications. Paper 26. http://ecommons.udayton.edu/bio_fac_pub/26 ...
... Tsonis, Panagiotis A., "A Comparative 2-Dimensional Gel Protein Database of the Intact and Regenerating Newt Limbs" (1993). Biology Faculty Publications. Paper 26. http://ecommons.udayton.edu/bio_fac_pub/26 ...
protein-complex_cros..
... CRLs - a Model for Protein Complex Ontology Development • CRLs offer full spectrum of complex and supra-complex structure/function complexity • Regulatory dimensions include: – Combinatorial complexity of complex formation across spatio-temporal domains – PTM of both complex and target substrates t ...
... CRLs - a Model for Protein Complex Ontology Development • CRLs offer full spectrum of complex and supra-complex structure/function complexity • Regulatory dimensions include: – Combinatorial complexity of complex formation across spatio-temporal domains – PTM of both complex and target substrates t ...
Structural analysis of bacterial virulence factors
... information about motility proteins, and we aim to address this gap in knowledge. We have recently determined the first crystal structure of the MotB domain that anchors the proton-motive-force generating mechanism of the motor to the cell wall, and formulated a model of how the stator attaches to p ...
... information about motility proteins, and we aim to address this gap in knowledge. We have recently determined the first crystal structure of the MotB domain that anchors the proton-motive-force generating mechanism of the motor to the cell wall, and formulated a model of how the stator attaches to p ...
RQ for Ex. 2
... 6. Rev protein binds to a sequence of bases in the retrovirus RNA. (It doesn’t bind to DNA.) The sequence that rev binds to is in the intron in the rev gene; the binding site is called the RRE (rev response element). A-1. Where should rev mRNA be translated? On ribosomes that are (attached to ER or ...
... 6. Rev protein binds to a sequence of bases in the retrovirus RNA. (It doesn’t bind to DNA.) The sequence that rev binds to is in the intron in the rev gene; the binding site is called the RRE (rev response element). A-1. Where should rev mRNA be translated? On ribosomes that are (attached to ER or ...
Chapter 9a - Richsingiser.com
... “Fat-Free Proteins” • Trypanosomiasis (sleeping sickness) is a fatal disease, prevalent in Africa and caused by the protozoan Trypanosoma brucei and similar organisms • No safe and effective drugs exist for this disease, but research has focused on the N-myristoyltransferase (NMT) that attaches myr ...
... “Fat-Free Proteins” • Trypanosomiasis (sleeping sickness) is a fatal disease, prevalent in Africa and caused by the protozoan Trypanosoma brucei and similar organisms • No safe and effective drugs exist for this disease, but research has focused on the N-myristoyltransferase (NMT) that attaches myr ...
Identifying On the lines provided, identify each
... __carbohydrates__ 1. the main source of energy for living things __proteins_______ 2. help carry out chemical reactions __lipids_________ 3. important parts of biological membranes __nucleic acids____ 4. found in viruses, which are nonliving __proteins_______ 5. transport substances in and out of ce ...
... __carbohydrates__ 1. the main source of energy for living things __proteins_______ 2. help carry out chemical reactions __lipids_________ 3. important parts of biological membranes __nucleic acids____ 4. found in viruses, which are nonliving __proteins_______ 5. transport substances in and out of ce ...
Biology 1406 Quiz 2 Multiple-Choice Questions 1) When biologists
... D) transferring electrons from organic molecules to pyruvate. E) generating carbon dioxide and oxygen in the electron transport chain. 46) During aerobic respiration, H2O is formed. Where does the oxygen atom for the formation of the water come from? A) carbon dioxide (CO2) B) glucose (C6H12O6) C) m ...
... D) transferring electrons from organic molecules to pyruvate. E) generating carbon dioxide and oxygen in the electron transport chain. 46) During aerobic respiration, H2O is formed. Where does the oxygen atom for the formation of the water come from? A) carbon dioxide (CO2) B) glucose (C6H12O6) C) m ...
Anton Supercomputer, a computational microscope.
... Determined for each protein how many folding pathways are traversed that are distinct in the sense that native interactions are formed in different orders and that the pathways do not interconvert on the transition path time scale. Examined the thermodynamics and kinetics of the folding process, and ...
... Determined for each protein how many folding pathways are traversed that are distinct in the sense that native interactions are formed in different orders and that the pathways do not interconvert on the transition path time scale. Examined the thermodynamics and kinetics of the folding process, and ...
Post-translational Modification by Ubiquitin and
... • The APP-BP1 N-terminal half is homologous to the Nterminal half of ubiquitin E1 • UBA3 is homologous to the C-terminal half of ubiquitin E1 and contains the cysteine required for thiol ester linkage with Nedd8 ...
... • The APP-BP1 N-terminal half is homologous to the Nterminal half of ubiquitin E1 • UBA3 is homologous to the C-terminal half of ubiquitin E1 and contains the cysteine required for thiol ester linkage with Nedd8 ...
Chapter Five - DORAS
... cultures by the water lysis method (section 2.14.2). Protein concentration was quantified by the Schaffner Weissmann protein assay (section 2.13) and results are shown in table 5.1. A 30µg sample of each preparation was run on an SDS-PAGE gel (section 2.15) with a Sigma SDS7 marker. Coomassie staini ...
... cultures by the water lysis method (section 2.14.2). Protein concentration was quantified by the Schaffner Weissmann protein assay (section 2.13) and results are shown in table 5.1. A 30µg sample of each preparation was run on an SDS-PAGE gel (section 2.15) with a Sigma SDS7 marker. Coomassie staini ...
Marvelous Macromolecules
... (long carbon skeleton) Joined by ester linkage in dehydration reaction Used in energy storage, cushion organs, and for insulation ...
... (long carbon skeleton) Joined by ester linkage in dehydration reaction Used in energy storage, cushion organs, and for insulation ...
Is host lipidation of pathogen effector proteins a general virulence
... necessary for the progression of infection. The molecular mechanisms by which these effectors support virulence have been the subject of much research. Recent papers analyzing the intracellular fate of Legionella pneumophila effectors have underlined the importance of their subcellular localization ...
... necessary for the progression of infection. The molecular mechanisms by which these effectors support virulence have been the subject of much research. Recent papers analyzing the intracellular fate of Legionella pneumophila effectors have underlined the importance of their subcellular localization ...
XL-I
... Hb demonstrates higher oxygen carrying capacity compared to myoglobin II. There is covalent bonding between the four subunits of Hb III. During deoxygenation the loss of the first oxygen molecule from oxygenated Hb promotes the dissociation of oxygen from the other subunits (A) II ...
... Hb demonstrates higher oxygen carrying capacity compared to myoglobin II. There is covalent bonding between the four subunits of Hb III. During deoxygenation the loss of the first oxygen molecule from oxygenated Hb promotes the dissociation of oxygen from the other subunits (A) II ...
A Story About Cakes
... 3. To make sure the recipe isn’t lost or tampered with, he photocopies it to go to the kitchen ...
... 3. To make sure the recipe isn’t lost or tampered with, he photocopies it to go to the kitchen ...
Journal of Bacteriology
... (3), the hybrid protein would be degraded under these conditions if the 1-lactamase portion were extending into the medium. Should the hybrid protein be degraded only in the presence of EDTA, which allows proteolytic attack also from the periplasmic side, the ,B-lactamase portion would face the peri ...
... (3), the hybrid protein would be degraded under these conditions if the 1-lactamase portion were extending into the medium. Should the hybrid protein be degraded only in the presence of EDTA, which allows proteolytic attack also from the periplasmic side, the ,B-lactamase portion would face the peri ...
Materials and Methods - UROP
... overnight culture was grown and induced with isopropyl-β-D-thiogalactopyranoside (IPTG) when the optical density of cells at 600 nm was 0.8. After four hours, the culture was centrifuged. The cell pellet was resuspended in phosphate buffered solution (PBS) and sonicated before centrifugation. The sa ...
... overnight culture was grown and induced with isopropyl-β-D-thiogalactopyranoside (IPTG) when the optical density of cells at 600 nm was 0.8. After four hours, the culture was centrifuged. The cell pellet was resuspended in phosphate buffered solution (PBS) and sonicated before centrifugation. The sa ...
Rabbit anti-Sigma-1 Receptor Rabbit anti-Sigma
... This polyclonal antibody is supplied as a 400 µL aliquot at a concentration of 0.25 mg/mL in phosphate buffered saline (pH 7.4) containing 0.1% sodium azide. This antibody is epitope-affinity purified from rabbit antiserum. PAD: ZMD.554 IMMUNOGEN Synthetic peptide derived from the C-terminal region ...
... This polyclonal antibody is supplied as a 400 µL aliquot at a concentration of 0.25 mg/mL in phosphate buffered saline (pH 7.4) containing 0.1% sodium azide. This antibody is epitope-affinity purified from rabbit antiserum. PAD: ZMD.554 IMMUNOGEN Synthetic peptide derived from the C-terminal region ...
4.5 Protein Purification Methods
... – Iso-electric focusing used in QC to identify two similar proteins that are difficult to separate by any other means • Each protein has a specific number of charged amino acids on its surface in specific places • Creates a unique electric signature known as its iso-electric point (IEP) where charge ...
... – Iso-electric focusing used in QC to identify two similar proteins that are difficult to separate by any other means • Each protein has a specific number of charged amino acids on its surface in specific places • Creates a unique electric signature known as its iso-electric point (IEP) where charge ...
The presentation
... • Proteins with nuclear AND extracellular domains excluded. • Multiple alignments and known locations of domains – definition of domains’ borders. • Automatic searches to find more members, Evalue < 0.1, and manual checks. • Marginal similarity to domain family – possible divergent family. ...
... • Proteins with nuclear AND extracellular domains excluded. • Multiple alignments and known locations of domains – definition of domains’ borders. • Automatic searches to find more members, Evalue < 0.1, and manual checks. • Marginal similarity to domain family – possible divergent family. ...
Plasma Membrane Structure and Function
... their concentration gradients (from low to high concentration). This is done by protein pumps embedded in the membrane. In contrast to passive transport, active transport requires energy in the form of ATP. ...
... their concentration gradients (from low to high concentration). This is done by protein pumps embedded in the membrane. In contrast to passive transport, active transport requires energy in the form of ATP. ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.