LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
... 06. TEMED acts as a catalyst in SDS-PAGE. 07. Starch is a homopolysaccharide. 08. Globular proteins are soluble in water. 09. An alcohol group in lecithin is glycerol. 10. IUB refers to international union of biochemistry. ...
... 06. TEMED acts as a catalyst in SDS-PAGE. 07. Starch is a homopolysaccharide. 08. Globular proteins are soluble in water. 09. An alcohol group in lecithin is glycerol. 10. IUB refers to international union of biochemistry. ...
macromolecules new
... The structure of proteins • The basic building blocks of proteins are called amino acids. • There are about 20 common amino acids that can make literally thousands of proteins. ...
... The structure of proteins • The basic building blocks of proteins are called amino acids. • There are about 20 common amino acids that can make literally thousands of proteins. ...
Carbohydrates
... • Triglycerides-- fats and oils --(made of glycerol and 3 fatty acids) • Phospholipids)—cell membrane-(made of glycerol, 2 fatty acids, and 1 phosphate group ...
... • Triglycerides-- fats and oils --(made of glycerol and 3 fatty acids) • Phospholipids)—cell membrane-(made of glycerol, 2 fatty acids, and 1 phosphate group ...
Application Note: Using the NanoDrop One to Quantify Protein and
... Researchers have always needed ways to quickly quantify various biomolecules (e.g., protein and nucleic acid preparations) as a routine part of their workflows. This information helps them make informed decisions before proceeding with downstream experiments. There are many protein quantification me ...
... Researchers have always needed ways to quickly quantify various biomolecules (e.g., protein and nucleic acid preparations) as a routine part of their workflows. This information helps them make informed decisions before proceeding with downstream experiments. There are many protein quantification me ...
View Full PDF - Biochemical Society Transactions
... from particular biological sources. The chapter on lysosomal enzymes has been almost completely rewritten, and contains extremely useful and comprehensive reviews on all of those enzymes currently considered to be associated with lysosomes. Relevant biochemical information concerning these enzymes, ...
... from particular biological sources. The chapter on lysosomal enzymes has been almost completely rewritten, and contains extremely useful and comprehensive reviews on all of those enzymes currently considered to be associated with lysosomes. Relevant biochemical information concerning these enzymes, ...
Classwork - Biomonsters
... nucleotides (see board), draw a mini DNA molecule. Your drawing should meet the following requirements: • Contain 6 nucleotides total • Label A, T, C, and Gs • Label the 5’ and 3’ ends of each DNA strand ...
... nucleotides (see board), draw a mini DNA molecule. Your drawing should meet the following requirements: • Contain 6 nucleotides total • Label A, T, C, and Gs • Label the 5’ and 3’ ends of each DNA strand ...
slides - ODU Computer Science
... function, stage of the cell A cool animation http://www.maxanim.com/genetics/cDNA/cDNA.htm ...
... function, stage of the cell A cool animation http://www.maxanim.com/genetics/cDNA/cDNA.htm ...
research abstract form
... Schistosomes are parasitic blood worms that infect over 200 million people and numerous wild and domestic animals worldwide. While the adult worms possess a functional mouth and gut, most nutrients are imported across the parasite’s body surface (or tegument). One protein complex that is important i ...
... Schistosomes are parasitic blood worms that infect over 200 million people and numerous wild and domestic animals worldwide. While the adult worms possess a functional mouth and gut, most nutrients are imported across the parasite’s body surface (or tegument). One protein complex that is important i ...
Materials and Methods - Philosophical Transactions of the Royal
... Supplementary material 1: Materials and methods used to obtain original data reported in this article Animals and embryos Mature adults of C. intestinalis were collected from harbors in Murotsu, Hyogo, Japan. The adults were maintained in indoor tanks of artificial seawater (Marine Art BR, Senju Sei ...
... Supplementary material 1: Materials and methods used to obtain original data reported in this article Animals and embryos Mature adults of C. intestinalis were collected from harbors in Murotsu, Hyogo, Japan. The adults were maintained in indoor tanks of artificial seawater (Marine Art BR, Senju Sei ...
protein review
... - Size, charge, polarity or hydrophobicity of R groups will affect the bonds present at higher levels and the ultimate 3-D conformation n function of protein ...
... - Size, charge, polarity or hydrophobicity of R groups will affect the bonds present at higher levels and the ultimate 3-D conformation n function of protein ...
Lecture 12
... • Protein Kinase A (PKA) – Alters activities of target proteins by phosphorylating specific Ser/Thr – Activated by cAMP – Mechanism of activation (allosteric) • R2C2 (in the absence of cAMP) – 2 catalytic unit, high affinity ...
... • Protein Kinase A (PKA) – Alters activities of target proteins by phosphorylating specific Ser/Thr – Activated by cAMP – Mechanism of activation (allosteric) • R2C2 (in the absence of cAMP) – 2 catalytic unit, high affinity ...
Solutions to 7.012 Problem Set 1
... a) Explain the following to Mike. i) What are lipids? The term lipid refers to a variety of molecules composed mainly of hydrocarbons. They are water insoluble due to the many non-polar covalent bonds. ii) Why are saturated fatty acids more likely to be solid at room temperature than ...
... a) Explain the following to Mike. i) What are lipids? The term lipid refers to a variety of molecules composed mainly of hydrocarbons. They are water insoluble due to the many non-polar covalent bonds. ii) Why are saturated fatty acids more likely to be solid at room temperature than ...
ImmunoTools special Award 2015
... which enable them to detach from tumor site, invade and colonize distinct tissues. This also involves the crosstalk between tumor cells, with the immune system, their source and target tissues. Most cell types secrete extracellular vesicles (EVs), a heterogenous population of membrane particles, nam ...
... which enable them to detach from tumor site, invade and colonize distinct tissues. This also involves the crosstalk between tumor cells, with the immune system, their source and target tissues. Most cell types secrete extracellular vesicles (EVs), a heterogenous population of membrane particles, nam ...
The exploitation of chromosome recombination between Lolium and
... The beginnings of genetic engineering date back to the 1970’s. Today this technology permits us to produce animals that manufacture human proteins – one of the most expensive medicines. Human proteins can even be made in bacteria, and this process is at present the simplest, cheapest and quickest me ...
... The beginnings of genetic engineering date back to the 1970’s. Today this technology permits us to produce animals that manufacture human proteins – one of the most expensive medicines. Human proteins can even be made in bacteria, and this process is at present the simplest, cheapest and quickest me ...
Gene regulation
... As you already know, genes control protein synthesis. Some proteins are required all the time by cells whilst others are only needed in specific circumstances and so are not made all the time. Such genes must be capable of being switched on or off – it would be wasteful if they were made and not req ...
... As you already know, genes control protein synthesis. Some proteins are required all the time by cells whilst others are only needed in specific circumstances and so are not made all the time. Such genes must be capable of being switched on or off – it would be wasteful if they were made and not req ...
Phosphorylation and concomitant structural changes in human 2
... a Q-Sepharose column, various forms of the hPrxI were separated by 12% SDS–PAGE gel and subjected to Western blotting with an anti-hPrxI antibody. Then we found that all the proteins showed similar apparent molecular mass of 21 kDa, which corresponded to the monomeric size of hPrxI (Fig. 1A, lower p ...
... a Q-Sepharose column, various forms of the hPrxI were separated by 12% SDS–PAGE gel and subjected to Western blotting with an anti-hPrxI antibody. Then we found that all the proteins showed similar apparent molecular mass of 21 kDa, which corresponded to the monomeric size of hPrxI (Fig. 1A, lower p ...
week2wkspans - Evergreen Archives
... It makes sense that the part of a membrane protein that spans the hydrophobic tails of a bilayer is itself hydrophobic, because only hydrophobic amino acids will be able to interact with the nonpolar lipid tails. These amino acids are hydrophobic: glycine, alanine, valine, leucine, isoleucine, methi ...
... It makes sense that the part of a membrane protein that spans the hydrophobic tails of a bilayer is itself hydrophobic, because only hydrophobic amino acids will be able to interact with the nonpolar lipid tails. These amino acids are hydrophobic: glycine, alanine, valine, leucine, isoleucine, methi ...
Data Supplement
... Total RNA was isolated using Tripure reagent (Roche). Random hexamers were used to reversetranscribe reactions using superscript II (Invitrogen). Real-time PCR was performed using SYBR green (Applied biosystems). Data for each transcript were normalized to both GAPDH and 18S rRNA as internal control ...
... Total RNA was isolated using Tripure reagent (Roche). Random hexamers were used to reversetranscribe reactions using superscript II (Invitrogen). Real-time PCR was performed using SYBR green (Applied biosystems). Data for each transcript were normalized to both GAPDH and 18S rRNA as internal control ...
Building phylogenetic trees
... Note also that, no claim was made that all oxygen storage mechanisms that exists on earth came from the same ancestor (though this mechanism is the most abundant). In other word, it is not obvious that an oxygen storage protein is necessarily a globin. There is at least one more known family (of myo ...
... Note also that, no claim was made that all oxygen storage mechanisms that exists on earth came from the same ancestor (though this mechanism is the most abundant). In other word, it is not obvious that an oxygen storage protein is necessarily a globin. There is at least one more known family (of myo ...
CCP4 - Software for Protein Structure Solution
... • Diffraction image spot intensities and phase information are required to construct electron density map of target protein • Phase information not given by diffraction images. Must be derived from other techniques – – Experimental Phasing: Based on comparison of X-ray data from two or more slightly ...
... • Diffraction image spot intensities and phase information are required to construct electron density map of target protein • Phase information not given by diffraction images. Must be derived from other techniques – – Experimental Phasing: Based on comparison of X-ray data from two or more slightly ...
Gene Section HNRNPK (heterogeneous nuclear ribonucleoprotein K)
... acids and proteins. It has been suggested that hnRNPK serves as a docking platform that facilitates the interaction between the molecular partners involved in the processes that compose gene expression, such as transcription and trans-lation regulation, mRNA processing and chromatin remodeling (Boms ...
... acids and proteins. It has been suggested that hnRNPK serves as a docking platform that facilitates the interaction between the molecular partners involved in the processes that compose gene expression, such as transcription and trans-lation regulation, mRNA processing and chromatin remodeling (Boms ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.