thèse - Université Evry Val d`Essonne
... oxidative phosphorylation (OXPHOS), twenty two tRNAs and three rRNAs, one small ribosomal subunit protein (Var1) is also of mitochondrial origin (Fig. I-2B). The complete sequencing of several mammalian mtDNAs, including human, also revealed a small number of proteins (thirteen in humans) that are s ...
... oxidative phosphorylation (OXPHOS), twenty two tRNAs and three rRNAs, one small ribosomal subunit protein (Var1) is also of mitochondrial origin (Fig. I-2B). The complete sequencing of several mammalian mtDNAs, including human, also revealed a small number of proteins (thirteen in humans) that are s ...
Src protein–tyrosine kinase structure and regulation
... SH3 domains (60 amino acid residues) bind to sequences that can adopt a left-handed helical conformation. These sequences are usually rich in proline. RPLPPLP is an optimal Class 1 Src SH3 ligand [2,14]. UPPLPXR is an optimal Class 2 Src SH3 ligand that binds with an inverted orientation on the SH3 ...
... SH3 domains (60 amino acid residues) bind to sequences that can adopt a left-handed helical conformation. These sequences are usually rich in proline. RPLPPLP is an optimal Class 1 Src SH3 ligand [2,14]. UPPLPXR is an optimal Class 2 Src SH3 ligand that binds with an inverted orientation on the SH3 ...
Protein Profile Characterization of Donkey Milk
... The donkey’s three major whey proteins are -lactalbumin, -lactoglobulin and lysozyme. Donkey’s milk -lactalbumin has two isoforms with different isoeletric point 31. Recently, it has been shown that -lactalbumin presents antiviral, antitumor, and anti-stress properties. In particular in human ...
... The donkey’s three major whey proteins are -lactalbumin, -lactoglobulin and lysozyme. Donkey’s milk -lactalbumin has two isoforms with different isoeletric point 31. Recently, it has been shown that -lactalbumin presents antiviral, antitumor, and anti-stress properties. In particular in human ...
European Journal of Biochemistry
... The adsorption constant of the irreversible adsorption of the bacteriophage K3 to Escherichia coli K12 bacteria is strongly dependent on the incubation temperature. Two inflection points are observed in an Arrhenius plot. For cells grown at 37 "C the inflection points are found at 20 "C and 28 "C wh ...
... The adsorption constant of the irreversible adsorption of the bacteriophage K3 to Escherichia coli K12 bacteria is strongly dependent on the incubation temperature. Two inflection points are observed in an Arrhenius plot. For cells grown at 37 "C the inflection points are found at 20 "C and 28 "C wh ...
Biochemistry - Textbooks Online
... of chemical reactions that occur in living cells and organisms. Life is a chemical process involving thousands of different reactions occurring in an organised manner. These are called metabolic reactions. The term Biochemistry was first introduced by the German Chemist Carl Neuberg in 1903. It take ...
... of chemical reactions that occur in living cells and organisms. Life is a chemical process involving thousands of different reactions occurring in an organised manner. These are called metabolic reactions. The term Biochemistry was first introduced by the German Chemist Carl Neuberg in 1903. It take ...
View - BioOne
... herbicides because they possess many characteristics of higher plants. Six days after the application of monosulfuron at 0.03 to 0.3 nmol L21 under laboratory conditions, growth of the nitrogen-fixing cyanobacteria Anabaena flos-aquae, Anabaena azollae, and Anabaena azotica was stimulated, but at hi ...
... herbicides because they possess many characteristics of higher plants. Six days after the application of monosulfuron at 0.03 to 0.3 nmol L21 under laboratory conditions, growth of the nitrogen-fixing cyanobacteria Anabaena flos-aquae, Anabaena azollae, and Anabaena azotica was stimulated, but at hi ...
Creatine kinase: The reactive cysteine is required for synergism but
... of the pH optimum to lower values was observed. Mutant Mib-CKs were severalfold more sensitive to inhibition by free ADP in the reverse reaction (ATP synthesis) and to free ATP in the forward reaction (phosphocreatine synthesis). With the exception of C278D, all mutant enzymes were specifically acti ...
... of the pH optimum to lower values was observed. Mutant Mib-CKs were severalfold more sensitive to inhibition by free ADP in the reverse reaction (ATP synthesis) and to free ATP in the forward reaction (phosphocreatine synthesis). With the exception of C278D, all mutant enzymes were specifically acti ...
Prying Into Prions - Colorado Parks and Wildlife
... Kingdom discovered a new variant of a human transmissible spongiform encephalopathy called Creutzfeldt-Jakob disease (CJD). CJD normally occurs in older people, but in 1996 a few teenagers started showing classic CJD symptoms. Biopsies of the brain tissue of these victims revealed that the new varia ...
... Kingdom discovered a new variant of a human transmissible spongiform encephalopathy called Creutzfeldt-Jakob disease (CJD). CJD normally occurs in older people, but in 1996 a few teenagers started showing classic CJD symptoms. Biopsies of the brain tissue of these victims revealed that the new varia ...
Full Text - the American Society of Animal Science
... myofibers and i.m. adipocytes could theoretically contribute to variation in i.m. fat level (Kelley and Goodpaster, 2001; Gondret et al., 2004). Thus, studies have elucidated several candidate genes, such as the adipocyte fatty-acid binding protein [(FABP)-4] and heart fatty-acid binding protein (FA ...
... myofibers and i.m. adipocytes could theoretically contribute to variation in i.m. fat level (Kelley and Goodpaster, 2001; Gondret et al., 2004). Thus, studies have elucidated several candidate genes, such as the adipocyte fatty-acid binding protein [(FABP)-4] and heart fatty-acid binding protein (FA ...
Hyaluronic acid influence on platelet-induced airway smooth muscle cell proliferation
... ASMC and platelets) were thereafter lysed by incubation in RIPA buffer supplemented with 1X phosphatase and protease inhibitor coctail for 30 min on ice followed by centrifugation at 14 000 g at 4˚C for 10 min. The supernatant was incubated with 0.5 µg FAK antibody for 2h at 4˚C followed by immunopr ...
... ASMC and platelets) were thereafter lysed by incubation in RIPA buffer supplemented with 1X phosphatase and protease inhibitor coctail for 30 min on ice followed by centrifugation at 14 000 g at 4˚C for 10 min. The supernatant was incubated with 0.5 µg FAK antibody for 2h at 4˚C followed by immunopr ...
Fundamental Challenges in Mechanistic Enzymology: Progress
... thought to be present in the transition state, and such ligands are often referred to as “transition state analogues”.35,36 These structural, functional, and binding observations support a common picture in which active sites are complementary in shape and electrostatic properties to transition stat ...
... thought to be present in the transition state, and such ligands are often referred to as “transition state analogues”.35,36 These structural, functional, and binding observations support a common picture in which active sites are complementary in shape and electrostatic properties to transition stat ...
Amino Acids, Proteins, and Enzymes
... Substrate Concentration and Reaction Rate • The rate of reaction increases as substrate concentration increases (at constant enzyme concentration) • Maximum activity occurs when the enzyme is saturated (when all enzymes are binding substrate) • The relationship between reaction rate and substrate c ...
... Substrate Concentration and Reaction Rate • The rate of reaction increases as substrate concentration increases (at constant enzyme concentration) • Maximum activity occurs when the enzyme is saturated (when all enzymes are binding substrate) • The relationship between reaction rate and substrate c ...
Chapter 10 Enzymes - Angelo State University
... • Because of the large number of enzymes that are now known, a systematic nomenclature called the Enzyme Commission (EC) system is used to name them. [International Union of Biochemistry and Molecular Biology] • Enzymes are grouped into six major classes on the basis of the reaction which they catal ...
... • Because of the large number of enzymes that are now known, a systematic nomenclature called the Enzyme Commission (EC) system is used to name them. [International Union of Biochemistry and Molecular Biology] • Enzymes are grouped into six major classes on the basis of the reaction which they catal ...
A family of diatom-like silicon transporters in the siliceous loricate
... from D. grandis (see the electronic supplementary material). A protein alignment of those contigs providing a significant hit was generated using CLUSTALX, and the longest contig analysed using INTERPROSCAN. Stephanoeca diplocostata dTcDNA was made using Superscript III First-Strand Synthesis revers ...
... from D. grandis (see the electronic supplementary material). A protein alignment of those contigs providing a significant hit was generated using CLUSTALX, and the longest contig analysed using INTERPROSCAN. Stephanoeca diplocostata dTcDNA was made using Superscript III First-Strand Synthesis revers ...
Chlamydia trachomatis RNA polymerase major sigma subunit
... in 5 ml of 25 mM Tris buffer (pH 7.5). Sample buffer containing P-ME and SDS was added to give a final concentration of 5% B-ME and 2% SDS. All samples were sonicated for 15 s and boiled for 3 min just before loading onto a 12% SDS-polyacrylamide gel. After electrophoresis, proteins were transferred ...
... in 5 ml of 25 mM Tris buffer (pH 7.5). Sample buffer containing P-ME and SDS was added to give a final concentration of 5% B-ME and 2% SDS. All samples were sonicated for 15 s and boiled for 3 min just before loading onto a 12% SDS-polyacrylamide gel. After electrophoresis, proteins were transferred ...
Protein and Amino Acid Metabolism During and After Exercise
... nuclei shared between cells; its ribosomes are not found arrayed on endoplasmic reticulum. It does not seem to possess morphologically well-defined lysosomes, such as are seen in other tissues, but nevertheless there is a full complement of ...
... nuclei shared between cells; its ribosomes are not found arrayed on endoplasmic reticulum. It does not seem to possess morphologically well-defined lysosomes, such as are seen in other tissues, but nevertheless there is a full complement of ...
THE ESTIMATION OF ACTIVE NATIVE TRYPSIN IN THE
... denatured hemoglobin by trypsin is allowed to take place in a slightly alkaline solution buffered with phosphate. Precipitation of the denatured hemoglobin is prevented by urea. The undigested hemoglobin is precipitated with trichloracetic acid. The digested hemoglobin not precipitated by trichlorac ...
... denatured hemoglobin by trypsin is allowed to take place in a slightly alkaline solution buffered with phosphate. Precipitation of the denatured hemoglobin is prevented by urea. The undigested hemoglobin is precipitated with trichloracetic acid. The digested hemoglobin not precipitated by trichlorac ...
Gene Section CD9 (CD9 molecule) Atlas of Genetics and Cytogenetics
... CD9 can interact or form complexes with many other proteins, including other tetraspanins, integrins, EWI molecules, TGF-a, diphtheria toxin receptor, receptor tyrosine kinase, pregnancy specific glycoproteins, and proteins of the immune system such as MHC class II molecules and members of the Ig su ...
... CD9 can interact or form complexes with many other proteins, including other tetraspanins, integrins, EWI molecules, TGF-a, diphtheria toxin receptor, receptor tyrosine kinase, pregnancy specific glycoproteins, and proteins of the immune system such as MHC class II molecules and members of the Ig su ...
... equilibrated with the same buffer, with a flow rate of 0.5mL/min. The single peak of antitryptic activity was collected and dialyzed against distilled water and lyophilized. The G-50 column fraction was then separated by reverse-phase HPLC, as described by Macedo et al. (2007), on a Hypersil Gold ...
LIPID MOBILIZATION
... • FA primarily enter a cell via fatty acid protein transporters on the cell surface – fatty acid translocase (FAT/CD36), – tissue specific fatty acid transport proteins (FATP), – plasma membrane bound fatty acid binding protein (FABPpm) ...
... • FA primarily enter a cell via fatty acid protein transporters on the cell surface – fatty acid translocase (FAT/CD36), – tissue specific fatty acid transport proteins (FATP), – plasma membrane bound fatty acid binding protein (FABPpm) ...
Case Study: BPTI
... no exception and due to its rather small overall size it also has a relatively small hydrophobic core. Thus, extra stability to support its three dimensional structure is provided by the three internal disulfide bonds that were discussed previously. It is, therefore, interesting to consider what wou ...
... no exception and due to its rather small overall size it also has a relatively small hydrophobic core. Thus, extra stability to support its three dimensional structure is provided by the three internal disulfide bonds that were discussed previously. It is, therefore, interesting to consider what wou ...
A comparison of scoring functions for protein sequence
... aligned pairs in the test alignment, tc, divided by the length of the reference alignment. This score has been used, for example, by Thompson et al. (1994), who call it SP, and by Sauder et al. (2000), who refer to it as fD, the developer's score. PS (reverse sum-of-pairs) is tc divided by the lengt ...
... aligned pairs in the test alignment, tc, divided by the length of the reference alignment. This score has been used, for example, by Thompson et al. (1994), who call it SP, and by Sauder et al. (2000), who refer to it as fD, the developer's score. PS (reverse sum-of-pairs) is tc divided by the lengt ...
Role of Na and K in Enzyme Function
... oil field brines, hydrothermal brines, sodic soils, and drying salt lakes may approach saturation levels of salt (306). The ability to thrive or require salt, halotolerance or halophily, requires a number of energetically expensive cellular adaptations that are typically complemented by photosynthet ...
... oil field brines, hydrothermal brines, sodic soils, and drying salt lakes may approach saturation levels of salt (306). The ability to thrive or require salt, halotolerance or halophily, requires a number of energetically expensive cellular adaptations that are typically complemented by photosynthet ...
Biotechnology and Bioengineering
... and a 957 bp DNA fragment that includes the SalI restriction site downstream of codon F176 was amplified using primers F176-front and ToMO-SalI-rear (Table I). Similarly, to perform saturation mutagenesis at TouA position F196, a 763 bp DNA fragment was amplified using primers ToMO-KpnI-front and F1 ...
... and a 957 bp DNA fragment that includes the SalI restriction site downstream of codon F176 was amplified using primers F176-front and ToMO-SalI-rear (Table I). Similarly, to perform saturation mutagenesis at TouA position F196, a 763 bp DNA fragment was amplified using primers ToMO-KpnI-front and F1 ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.