ANP 204 Main Text - National Open University of Nigeria
... glucose but for a change in the positions of OH group on carbon 2 and 4 for mannose and galactose respectively. The carbon atoms involved are ...
... glucose but for a change in the positions of OH group on carbon 2 and 4 for mannose and galactose respectively. The carbon atoms involved are ...
as PDF
... that the non-globular part of the molecule is masking this site. Proteolytic processing of adiponectin, which generates gAcrp30, removes the non-globular part and exposes the AdipoR1 binding-site. Since gAcrp30 exhibits biological activity distinct from that of full-length adiponectin, especially in ...
... that the non-globular part of the molecule is masking this site. Proteolytic processing of adiponectin, which generates gAcrp30, removes the non-globular part and exposes the AdipoR1 binding-site. Since gAcrp30 exhibits biological activity distinct from that of full-length adiponectin, especially in ...
Identification of a structural motif that confers specific interaction with
... the Gal4 system. The amino acid-substituted COP1 proteins expressed as well as wild-type COP1 in both two-hybrid systems (data not shown). The use of different two-hybrid systems prevents a direct comparison between the HY5 and the STO/STH proteins; however, the relative effect that the WD40 domain ...
... the Gal4 system. The amino acid-substituted COP1 proteins expressed as well as wild-type COP1 in both two-hybrid systems (data not shown). The use of different two-hybrid systems prevents a direct comparison between the HY5 and the STO/STH proteins; however, the relative effect that the WD40 domain ...
A Bifunctional tRNA for In Vitro Selection
... an aminoacyl tRNA to become attached to their protein products. We have been developing an alternative method for generating libraries of mRNA-encoded peptides, which also has the potential to work with other peptide-like polymers. In our system, a modified tRNA (tRNAx) acts as a bifunctional crossl ...
... an aminoacyl tRNA to become attached to their protein products. We have been developing an alternative method for generating libraries of mRNA-encoded peptides, which also has the potential to work with other peptide-like polymers. In our system, a modified tRNA (tRNAx) acts as a bifunctional crossl ...
reprint - Charles Brenner Laboratory
... coA for the a-ketoglutarate dehydrogenase (a-KGDH) complex to form succinyl-coA. In addition, there must be GDP or ADP for the succinyl-coA synthetase reaction to form succinate, and there must be a supply of each citric acid cycle intermediate to keep the cycle running. Fuel oxidation is coupled to ...
... coA for the a-ketoglutarate dehydrogenase (a-KGDH) complex to form succinyl-coA. In addition, there must be GDP or ADP for the succinyl-coA synthetase reaction to form succinate, and there must be a supply of each citric acid cycle intermediate to keep the cycle running. Fuel oxidation is coupled to ...
22nd EMC Full Program - 25th Enzyme Mechanisms Conference
... enrich for a pool of hits, every screen is prone to nonspecific inhibition. Therefore, the hit-tolead phase requires a variety of approaches that deconvolute the initial hits. The goal is to validate hits that are specific, reversible and bind with a stoichiometry consistent with the mode of action. ...
... enrich for a pool of hits, every screen is prone to nonspecific inhibition. Therefore, the hit-tolead phase requires a variety of approaches that deconvolute the initial hits. The goal is to validate hits that are specific, reversible and bind with a stoichiometry consistent with the mode of action. ...
The acetaminophen metabolite
... production and its degradation. This occurs in two very rare inherited enzyme disorders of the γ-glutamyl cycle with low activities of either GS or 5-oxoprolinase (Njålsson et al., 2005; Ristoff and Larsson, 2007). In GS deficiency, intracellular glutathione is reduced to 5% to 21%. Inhibition of γ- ...
... production and its degradation. This occurs in two very rare inherited enzyme disorders of the γ-glutamyl cycle with low activities of either GS or 5-oxoprolinase (Njålsson et al., 2005; Ristoff and Larsson, 2007). In GS deficiency, intracellular glutathione is reduced to 5% to 21%. Inhibition of γ- ...
The Plant Cell - Department of Biology
... used in experiments to determine its heat stability, its ability to bind a hydrophobic matrix (Phenyl Sepharose), and its Ca 2 binding activity. All of these characteristics have been reported for several Ca2 binding proteins. Crude extract containing KIC was heated to 95C, cooled on ice, and cen ...
... used in experiments to determine its heat stability, its ability to bind a hydrophobic matrix (Phenyl Sepharose), and its Ca 2 binding activity. All of these characteristics have been reported for several Ca2 binding proteins. Crude extract containing KIC was heated to 95C, cooled on ice, and cen ...
Organic Chemistry Notes Student
... A. Carbohydrates - ______________________________________ Monosaccharides are the simplest ______________________. • The carbohydrate monomers are ___________________ • A monosaccharide has a formula that is a multiple of CH2O ______________________ ...
... A. Carbohydrates - ______________________________________ Monosaccharides are the simplest ______________________. • The carbohydrate monomers are ___________________ • A monosaccharide has a formula that is a multiple of CH2O ______________________ ...
Attachment 2 - Food Standards Australia New Zealand
... Sugar beet line H7-1 contains the cp4 epsps gene from Agrobacterium sp. strain CP4, which encodes the enzyme 5-enolpyruvylshikimate-3-phosphate synthase (CP4 EPSPS). The CP4 EPSPS enzyme is structurally and functionally similar to native plant EPSPS enzymes, but has a lower affinity for the herbicid ...
... Sugar beet line H7-1 contains the cp4 epsps gene from Agrobacterium sp. strain CP4, which encodes the enzyme 5-enolpyruvylshikimate-3-phosphate synthase (CP4 EPSPS). The CP4 EPSPS enzyme is structurally and functionally similar to native plant EPSPS enzymes, but has a lower affinity for the herbicid ...
Progesterone-stimulated intracellular calcium increase in human
... diluted in equal volume of 23 SB (Laemmli’s sample buffer 5 62.5 mM Tris pH 6.8, containing 10% glycerol, 20% SDS, 2.5% pyronin and 200 mM dithiothreitol), incubated at 95°C for 5 min and loaded onto 8%, 10% polyacrylamide–bisacrylamide gels. After SDS– PAGE, proteins were transferred to nitro-cellu ...
... diluted in equal volume of 23 SB (Laemmli’s sample buffer 5 62.5 mM Tris pH 6.8, containing 10% glycerol, 20% SDS, 2.5% pyronin and 200 mM dithiothreitol), incubated at 95°C for 5 min and loaded onto 8%, 10% polyacrylamide–bisacrylamide gels. After SDS– PAGE, proteins were transferred to nitro-cellu ...
to this tutorial as a PDF
... collection of atoms that you are interested in. Once you are comfortable using the basic Select command, you can begin to link together selections using Boolean Operators. To understand how boolean operators work, imagine that you are working with two predefined selection types, helices and backbone ...
... collection of atoms that you are interested in. Once you are comfortable using the basic Select command, you can begin to link together selections using Boolean Operators. To understand how boolean operators work, imagine that you are working with two predefined selection types, helices and backbone ...
Automated extraction of mutation data from the literature: application
... the Swiss-Prot entries are already present in two protein family databases that we previously implemented: the GPCRDB (Horn et al., 2003) and the NucleaRDB (Horn et al., 2001). For a new protein family, one can also use MuteXt to import Swiss-Prot entries. Name dictionary The dictionary is generated ...
... the Swiss-Prot entries are already present in two protein family databases that we previously implemented: the GPCRDB (Horn et al., 2003) and the NucleaRDB (Horn et al., 2001). For a new protein family, one can also use MuteXt to import Swiss-Prot entries. Name dictionary The dictionary is generated ...
BIOSENSOR (General principles and applications)
... •Photometric for light intensity Photon output for a luminescent or fluorescent process can be detected with photomultiplier tubes or photodiode systems. ...
... •Photometric for light intensity Photon output for a luminescent or fluorescent process can be detected with photomultiplier tubes or photodiode systems. ...
The AP-3 adaptor complex is required for vacuolar function
... compartments including PVC and vacuole [29]. The staining pattern of this probe in the root cells of pat4 mutants showed pronounced differences as compared to the control (Figure 2M-2S). The provacuole-like tubular extensions typically visible in the control [30], were largely absent in the pat4 mut ...
... compartments including PVC and vacuole [29]. The staining pattern of this probe in the root cells of pat4 mutants showed pronounced differences as compared to the control (Figure 2M-2S). The provacuole-like tubular extensions typically visible in the control [30], were largely absent in the pat4 mut ...
Mitochondria and energy production
... not implicate this protein in the inhibition of macroautophagy; rather, it should be taken as indicating an efficient regulatory system in which mTOR activation plays a role in both the stimulation of protein synthesis and the suppression of proteolysis. There is also evidence for an mTOR-independen ...
... not implicate this protein in the inhibition of macroautophagy; rather, it should be taken as indicating an efficient regulatory system in which mTOR activation plays a role in both the stimulation of protein synthesis and the suppression of proteolysis. There is also evidence for an mTOR-independen ...
Unit 14 – Biomolecules
... tertiary structures of protein are destroyed, but the primary structures remain unaltered. It can be said that during denaturation, secondary and tertiary-structured proteins get converted into ...
... tertiary structures of protein are destroyed, but the primary structures remain unaltered. It can be said that during denaturation, secondary and tertiary-structured proteins get converted into ...
Evidence for chloroplast control of external Ca -induced
... (Melis and Zeiger, 1982; Talbott and Zeiger, 1993; Tallman and Zeiger, 1988; Tominaga et al., 2001). However, the role of guard cell chloroplasts in signal transduction pathways is not clear. In plant cells, Ca2+ has a key role as a second messenger in intracellular signal transduction in a range of ...
... (Melis and Zeiger, 1982; Talbott and Zeiger, 1993; Tallman and Zeiger, 1988; Tominaga et al., 2001). However, the role of guard cell chloroplasts in signal transduction pathways is not clear. In plant cells, Ca2+ has a key role as a second messenger in intracellular signal transduction in a range of ...
Formate Dehydrogenase, Molecular Modeling and Docking with
... interaction toward NAD+ with grid score of -60.23, which is considered as an appropriate score for binding. After surfacing the 3D-structure of enzyme regions interacting with NAD+, we observed that FDH forms a pocket interaction shape with NAD+. The shape of FDH active site comprised with Pro 68, A ...
... interaction toward NAD+ with grid score of -60.23, which is considered as an appropriate score for binding. After surfacing the 3D-structure of enzyme regions interacting with NAD+, we observed that FDH forms a pocket interaction shape with NAD+. The shape of FDH active site comprised with Pro 68, A ...
Chapter 22
... • These are examples of emulsions which are combinations of two liquids that ordinarily will not stay mixed. • These liquids are usually oil and vinegar, lemon juice or another water based liquid • Temporary emulsion: also known as French, you must shake or stir this to mix this dressing each time y ...
... • These are examples of emulsions which are combinations of two liquids that ordinarily will not stay mixed. • These liquids are usually oil and vinegar, lemon juice or another water based liquid • Temporary emulsion: also known as French, you must shake or stir this to mix this dressing each time y ...
FLAVIN MONONUCLEOTIDE PHOSPHATASE FROM GOAT LIVER: A POSSIBLE TARGET FOR
... phosphatase which converts FMN into riboflavin. The existence of FMN-phosphatase has been shown in several plants as well as in animal organs. Enzyme reactions are inhibited by metals which may form complex with the substrate, or combine with the protein-active group of the enzymes, or react with th ...
... phosphatase which converts FMN into riboflavin. The existence of FMN-phosphatase has been shown in several plants as well as in animal organs. Enzyme reactions are inhibited by metals which may form complex with the substrate, or combine with the protein-active group of the enzymes, or react with th ...
Journal of Bacteriology
... reverse transcriptase (Pharmacia) annealed on approximately 20 mg of RNA at 458C. To prevent self-copying of labeled primer, actinomycin D was added to the reaction mixture in a final concentration of 50 mg/ml. Preparation of protein samples, electrophoresis, and Western blotting (immunoblotting). C ...
... reverse transcriptase (Pharmacia) annealed on approximately 20 mg of RNA at 458C. To prevent self-copying of labeled primer, actinomycin D was added to the reaction mixture in a final concentration of 50 mg/ml. Preparation of protein samples, electrophoresis, and Western blotting (immunoblotting). C ...
DIFFERENCES IN ENZYME CONTENT OF AZUROPHIL AND
... (21) glutaraldehyde in 0.1 M sodium cacodylate buffer, pH 7.4 (22), with 1% sucrose, 15 min, followed by washing in the same buffer with 7% sucrose. Other fixatives were used when recommended in the original procedure (see Table I). Fixation was carried out at 4 ° C. Specimens were then briefly wash ...
... (21) glutaraldehyde in 0.1 M sodium cacodylate buffer, pH 7.4 (22), with 1% sucrose, 15 min, followed by washing in the same buffer with 7% sucrose. Other fixatives were used when recommended in the original procedure (see Table I). Fixation was carried out at 4 ° C. Specimens were then briefly wash ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.