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Isoenzymes in Clinical Diagnosis
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Ces locus embedded proteins control the non
... network controlling cereulide synthesis on a transcriptional level, while the ABC transporter CesCD was found to be essential for post-translational control of cereulide synthesis. Using a gene inactivation approach, we show that the NRPS activating function of the phosphopantetheinyl transferase (P ...
... network controlling cereulide synthesis on a transcriptional level, while the ABC transporter CesCD was found to be essential for post-translational control of cereulide synthesis. Using a gene inactivation approach, we show that the NRPS activating function of the phosphopantetheinyl transferase (P ...
Plant Peroxisomes: Biogenesis and Function
... A Historical Perspective The biogenetic relationship between the endoplasmic reticulum (ER) and peroxisomes has been highly contentious (reviewed in Mullen and Trelease, 2006; Schlüter et al., 2006; Tabak et al., 2006). Peroxisomes were initially thought to form exclusively by vesiculation of specia ...
... A Historical Perspective The biogenetic relationship between the endoplasmic reticulum (ER) and peroxisomes has been highly contentious (reviewed in Mullen and Trelease, 2006; Schlüter et al., 2006; Tabak et al., 2006). Peroxisomes were initially thought to form exclusively by vesiculation of specia ...
RuBisCO in Non-Photosynthetic Alga Euglena longa: Divergent
... II RuBisCO). In these lineages, the Rbc gene was acquired from a bacterium by horizontal gene transfer [3]. In all other cases, the large subunit, possessing the catalytic activity of the holoenzyme, is always encoded in the plastid genome by the rbcL gene and synthesized on the plastid ribosomes in ...
... II RuBisCO). In these lineages, the Rbc gene was acquired from a bacterium by horizontal gene transfer [3]. In all other cases, the large subunit, possessing the catalytic activity of the holoenzyme, is always encoded in the plastid genome by the rbcL gene and synthesized on the plastid ribosomes in ...
Identification of the Factors Responsible for the Interaction of
... out all forms of docking such as rigid, flexible and semi flexible docking. It calculates the intermolecular “energies” by adding up all intermolecular interactions (e.g. Vander Waals, electrostatic) that occur between a ligand and protein targe. Hex Server has an easy-to-use form-based interface, t ...
... out all forms of docking such as rigid, flexible and semi flexible docking. It calculates the intermolecular “energies” by adding up all intermolecular interactions (e.g. Vander Waals, electrostatic) that occur between a ligand and protein targe. Hex Server has an easy-to-use form-based interface, t ...
Molecular cloning, characterization and gene expression of an
... Jesu Arockiaraj a, Sarasvathi Easwvaran a, Puganeshwaran Vanaraja a, Arun Singh b, ...
... Jesu Arockiaraj a, Sarasvathi Easwvaran a, Puganeshwaran Vanaraja a, Arun Singh b, ...
CARBOHYDRATE METABOLISM
... levels are high, conserving glucose for other tissues that use it as a primary fuel (e.g., brain, heart, white muscle) when blood glucose levels are low. The non-productinhibition of glucokinase allows the liver to capture glucose for storage as glycogen even when its intracellular levels of glucose ...
... levels are high, conserving glucose for other tissues that use it as a primary fuel (e.g., brain, heart, white muscle) when blood glucose levels are low. The non-productinhibition of glucokinase allows the liver to capture glucose for storage as glycogen even when its intracellular levels of glucose ...
The aconitase of Escherichia cok purification of the
... template) and the 456 bp PstI-EcoRI fragment was released and then purified by electrophoresis in 0.5 % agarose. Hybridizations were for 16h at 55, 60 and 65 "C, the filters were washed twice at the hybridization temperature for 15 rnin with 2 x SSC solution (17.53 g NaCl and 8-82g sodium citrate in ...
... template) and the 456 bp PstI-EcoRI fragment was released and then purified by electrophoresis in 0.5 % agarose. Hybridizations were for 16h at 55, 60 and 65 "C, the filters were washed twice at the hybridization temperature for 15 rnin with 2 x SSC solution (17.53 g NaCl and 8-82g sodium citrate in ...
Chemical and organic fertilizers affect physiological performance
... hundreds of host plants. In the current study, zinc sulfate and vermicompost as chemical and organic fertilizers, were added into cultural soil of Capsicum annuum to determine their effects on physiology and antioxidant activities of M. persicae. The aphids reared on zinc sulfate-treated culture sho ...
... hundreds of host plants. In the current study, zinc sulfate and vermicompost as chemical and organic fertilizers, were added into cultural soil of Capsicum annuum to determine their effects on physiology and antioxidant activities of M. persicae. The aphids reared on zinc sulfate-treated culture sho ...
Cell Division: The Place and Time of Cytokinesis Dispatch
... and are essential for cytokinesis [12,13]. The distribution of RacGAP50C throughout mitosis is highly dynamic. As shown by immunofluorescence: in prophase RacGAP50C is localized to the cytoplasm; during metaphase it is found over the mitotic spindle; just prior to the onset of furrow formation it is ...
... and are essential for cytokinesis [12,13]. The distribution of RacGAP50C throughout mitosis is highly dynamic. As shown by immunofluorescence: in prophase RacGAP50C is localized to the cytoplasm; during metaphase it is found over the mitotic spindle; just prior to the onset of furrow formation it is ...
Quantitative Proteomics of Transporter Expression in Brain Capillary
... The homogenate was mixed with 2 volumes of 32% Ficoll (final concentration, 16%) and centrifuged at 5800g for 20 min at 4°C. The resulting pellet was suspended in PBS buffer containing 1% bovine serum albumin solution and passed over a 0.4-mm glass bead column. Capillaries adhering to the glass bead ...
... The homogenate was mixed with 2 volumes of 32% Ficoll (final concentration, 16%) and centrifuged at 5800g for 20 min at 4°C. The resulting pellet was suspended in PBS buffer containing 1% bovine serum albumin solution and passed over a 0.4-mm glass bead column. Capillaries adhering to the glass bead ...
Sensitivity of plant mitochondrial terminal oxidases to the lipid
... effect of HNE on the respiratory chain in plant mitochondria, based on assays of succinate-dependent oxygen consumption, Cox and NADH-FeCN reductase in isolated potato mitochondria [9]. Unlike its animal counterpart, the plant mitochondrial respiratory electron transport chain contains two terminal ...
... effect of HNE on the respiratory chain in plant mitochondria, based on assays of succinate-dependent oxygen consumption, Cox and NADH-FeCN reductase in isolated potato mitochondria [9]. Unlike its animal counterpart, the plant mitochondrial respiratory electron transport chain contains two terminal ...
Full-Text PDF
... Currently, it is still in most cases impossible to purify and describe all of the major and minor allergens of natural allergen sources that are active in aqueous buffers. Thanks to the advance in the field of molecular genetics, this problem can be overcome by using cDNAs for recombinant production ...
... Currently, it is still in most cases impossible to purify and describe all of the major and minor allergens of natural allergen sources that are active in aqueous buffers. Thanks to the advance in the field of molecular genetics, this problem can be overcome by using cDNAs for recombinant production ...
From Sequence to Structure
... Hydrophobic amino-acid residues engage in van der Waals interactions only. Their tendency to avoid contact with water and pack against each other is the basis for the hydrophobic effect. Alanine and leucine are strong helix-favoring residues, while proline is rarely found in helices because its back ...
... Hydrophobic amino-acid residues engage in van der Waals interactions only. Their tendency to avoid contact with water and pack against each other is the basis for the hydrophobic effect. Alanine and leucine are strong helix-favoring residues, while proline is rarely found in helices because its back ...
Document
... Note: activity of these immunoprecip Was tested with no differences against CKII peptide. Post-translational modification may effect localization, substrate specificity, binding Partners, ect. (SH2 domain containing protein ...
... Note: activity of these immunoprecip Was tested with no differences against CKII peptide. Post-translational modification may effect localization, substrate specificity, binding Partners, ect. (SH2 domain containing protein ...
Enzymes and Vitamins Chapter 21 Problem
... Enzymes differ from inorganic laboratory catalysts in two ways: they are larger in size, and their activity is regulated by other substances. ...
... Enzymes differ from inorganic laboratory catalysts in two ways: they are larger in size, and their activity is regulated by other substances. ...
Rubisco Synthesis, Assembly, Mechanism, and Regulation
... to a more efficient form I enzyme, particularly under conditions in which low CC>2 prevails. Form I Rubisco is composed of two different-sized subunits. In addition to eight L-subunits, the enzyme requires an equivalent number of S-subunits to be fully active. Although the quaternary structures of t ...
... to a more efficient form I enzyme, particularly under conditions in which low CC>2 prevails. Form I Rubisco is composed of two different-sized subunits. In addition to eight L-subunits, the enzyme requires an equivalent number of S-subunits to be fully active. Although the quaternary structures of t ...
Hydrogel-Encapsulated Fluorophore-Enzyme Conjugates for Direct
... mining the “total anticholinesterase activity” of samples. While sensitive, current approaches are limited in specificity because cholinesterases are the target of a wide variety of toxic inhibitors, from heavy metals to chemical warfare agents. Widely used carbamate pesticides also inhibit AchE,6 w ...
... mining the “total anticholinesterase activity” of samples. While sensitive, current approaches are limited in specificity because cholinesterases are the target of a wide variety of toxic inhibitors, from heavy metals to chemical warfare agents. Widely used carbamate pesticides also inhibit AchE,6 w ...
Colicins produced by the Escherichia fergusonii strains closely
... EF3) was isolated after insertion of Tn7 into the plasmid backbone, resulting in the colicinogenic plasmid DS300. The colicin E1 cea gene encoded by this plasmid had a one-nucleotide replacement (G211A) when compared to cea of pColE1-EF43, resulting in a one-amino acid change (A71T) in colicin E1 pr ...
... EF3) was isolated after insertion of Tn7 into the plasmid backbone, resulting in the colicinogenic plasmid DS300. The colicin E1 cea gene encoded by this plasmid had a one-nucleotide replacement (G211A) when compared to cea of pColE1-EF43, resulting in a one-amino acid change (A71T) in colicin E1 pr ...
Detection of genetically modified plants
... detecting the inserted DNA, immunological assays detecting the resulting protein (e.g. the enzyme-linked immunoassay (ELISA) and lateral flow sticks), or using bioassays to detect the resultant phenotype (e.g. herbicide bioassays). Although much progress has been achieved in the development of genet ...
... detecting the inserted DNA, immunological assays detecting the resulting protein (e.g. the enzyme-linked immunoassay (ELISA) and lateral flow sticks), or using bioassays to detect the resultant phenotype (e.g. herbicide bioassays). Although much progress has been achieved in the development of genet ...
The initiation phase of protein synthesis in eukaryotes
... MNK1= kinase-interacting protein kinase inhibitors (in italics) used to delineate the pathways. Both MNK1 and eIF4E interact with eIF4G, bringing the two proteins in close proximity, resulting in more efficient eIF4E phosphorylation. ...
... MNK1= kinase-interacting protein kinase inhibitors (in italics) used to delineate the pathways. Both MNK1 and eIF4E interact with eIF4G, bringing the two proteins in close proximity, resulting in more efficient eIF4E phosphorylation. ...
A Raman spectroscopic study of the interaction between nucleotides
... polynucleotides poly(rA), poly(dA), poly(dT), poly(rU), and poly(rC), and with the oligonucleotides (dA), and (dA),, were recorded and interpreted. According to an analysis of the gp32 spectra with the reference intensity profiles of Alix and co-workers [M. Berjot, L. Marx, and A. J. P. Alix (1985) ...
... polynucleotides poly(rA), poly(dA), poly(dT), poly(rU), and poly(rC), and with the oligonucleotides (dA), and (dA),, were recorded and interpreted. According to an analysis of the gp32 spectra with the reference intensity profiles of Alix and co-workers [M. Berjot, L. Marx, and A. J. P. Alix (1985) ...
Non-ideal Plastic Behavior
... assign locations (critical control points) within the manufacturing process where the properties of the food must be measured to ensure that safety and quality are maintained, and to specify the appropriate action to take if a problem is identified. The type of analytical technique required to carry ...
... assign locations (critical control points) within the manufacturing process where the properties of the food must be measured to ensure that safety and quality are maintained, and to specify the appropriate action to take if a problem is identified. The type of analytical technique required to carry ...
Western blot
![](https://commons.wikimedia.org/wiki/Special:FilePath/Anti-lipoic_acid_immunoblot.png?width=300)
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.