Classification of pseudo pairs between nucleotide bases and amino
... necessary to enhance our knowledge of the complex recognition mechanisms underlying all those biological processes. A number of statistical and structural analyses of DNA–protein (12–20) and RNA–protein complexes (20–24) have been performed to determine such recognition principles. Essentially, basi ...
... necessary to enhance our knowledge of the complex recognition mechanisms underlying all those biological processes. A number of statistical and structural analyses of DNA–protein (12–20) and RNA–protein complexes (20–24) have been performed to determine such recognition principles. Essentially, basi ...
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... to embed this bacteria within the biofilm matrix. SRB has been reported to exist in biofilm matrices that are electroactive [32] or are determined to impart localized biocorrosion [2,33,34]. The biofilm matrix not only serves to adhere SRB onto surfaces but also hinders penetration of biocides. This ...
... to embed this bacteria within the biofilm matrix. SRB has been reported to exist in biofilm matrices that are electroactive [32] or are determined to impart localized biocorrosion [2,33,34]. The biofilm matrix not only serves to adhere SRB onto surfaces but also hinders penetration of biocides. This ...
Modifying the chain-length selectivity of the
... acyl-binding amino acid residues the only difference between the B.cepacia KWI-56 lipase (Figure 1) and BCL is that the former contains Leu instead of Val at the 266th position. A molecular model of BCL docked with saturated fatty acid methyl esters in the tetrahedral intermediate state was built by ...
... acyl-binding amino acid residues the only difference between the B.cepacia KWI-56 lipase (Figure 1) and BCL is that the former contains Leu instead of Val at the 266th position. A molecular model of BCL docked with saturated fatty acid methyl esters in the tetrahedral intermediate state was built by ...
Identification and Structural Characterization of the ATP/ADP
... GP96 or endoplasmin) localized to the endoplasmic reticulum, where it is involved in the assembly of immunoglobulins (Melnick et al., 1992) and other proteins destined for secretion or surface presentation. GRP94/ GP96 is also involved in the loading of proteosomegenerated antigenic peptides onto na ...
... GP96 or endoplasmin) localized to the endoplasmic reticulum, where it is involved in the assembly of immunoglobulins (Melnick et al., 1992) and other proteins destined for secretion or surface presentation. GRP94/ GP96 is also involved in the loading of proteosomegenerated antigenic peptides onto na ...
Journal of Biological Chemistry
... hexahistidyl tag under the tightly controlled araBAD promoter. ZntA was subcloned into the expression vector pBAD/Myc-His C by polymerase chain reaction methods. An NcoI site was generated at the initiation codon, and an EcoRI site was generated downstream of the termination codon using the oligonuc ...
... hexahistidyl tag under the tightly controlled araBAD promoter. ZntA was subcloned into the expression vector pBAD/Myc-His C by polymerase chain reaction methods. An NcoI site was generated at the initiation codon, and an EcoRI site was generated downstream of the termination codon using the oligonuc ...
... construct pCATGUS encoding the reporter proteins CAT and GUS, in a single ORF, produces a band corresponding to the expected [CATGUS] product. Other high molecular mass translation products correspond to N-terminally truncated forms of the protein (see below). Inclusion of the FMDV 2A sequence into ...
dbPSP: a curated database for protein phosphorylation sites in
... These authors contributed equally to this work. ...
... These authors contributed equally to this work. ...
Predicting the sidechain dihedral angle distributions
... The side-chain dihedral angle distributions of all amino acids have been measured from myriad high-resolution protein crystal structures. However, we do not yet know the dominant interactions that determine these distributions. Here, we explore to what extent the defining features of the side-chain ...
... The side-chain dihedral angle distributions of all amino acids have been measured from myriad high-resolution protein crystal structures. However, we do not yet know the dominant interactions that determine these distributions. Here, we explore to what extent the defining features of the side-chain ...
Міністерство охорони здоров`я України Харківський
... atom in all of the standard amino acids except glycine, where the R group is a hydrogen atom. This means that the structure of 19 from 20 standard amino acids posess a chiral center. With few exceptions (in some bacteria), the amino acids found in nature and in proteins are L-isomers. Two of the 19 ...
... atom in all of the standard amino acids except glycine, where the R group is a hydrogen atom. This means that the structure of 19 from 20 standard amino acids posess a chiral center. With few exceptions (in some bacteria), the amino acids found in nature and in proteins are L-isomers. Two of the 19 ...
GUIDE (download pdf)
... Workshop MRMA: Laboratory sessions 11 Universitat Autònoma de Barcelona If swabs samples are taken from surfaces which have been disinfected, there may still be traces of biocide which can react with and reduce the count of microorganisms present at the time of sampling. Such microorganisms may hav ...
... Workshop MRMA: Laboratory sessions 11 Universitat Autònoma de Barcelona If swabs samples are taken from surfaces which have been disinfected, there may still be traces of biocide which can react with and reduce the count of microorganisms present at the time of sampling. Such microorganisms may hav ...
Protein Feature Identification
... Protein Annotation • Objective - identify and describe all the physico-chemical, functional and structural properties of a protein including its sequence, accession #, mass, pI, absorptivity, solubility, active sites, binding sites, reactions, substrates, homologues, function, name(s), abundance, l ...
... Protein Annotation • Objective - identify and describe all the physico-chemical, functional and structural properties of a protein including its sequence, accession #, mass, pI, absorptivity, solubility, active sites, binding sites, reactions, substrates, homologues, function, name(s), abundance, l ...
Conversion of trypsin to a functional threonine protease
... several Tn variants were constructed in which the disulfide bond was removed and Ser 195 was substituted with threonine. The C42A/C58V/S195T-Tn variant (AVT-Tn) was designed to remove the Cys 42–Cys 58 disulfide bond and introduce a hydrophobic character into the modified region. This change was des ...
... several Tn variants were constructed in which the disulfide bond was removed and Ser 195 was substituted with threonine. The C42A/C58V/S195T-Tn variant (AVT-Tn) was designed to remove the Cys 42–Cys 58 disulfide bond and introduce a hydrophobic character into the modified region. This change was des ...
Enzymes
... 2. Enzymes are Stereospecific. If a molecule exists as a pair of enantiomers, the enzyme will use only one of the pair as substrate and produce only one of the pair as the product. For example, the enzymes that are involved in amino acid metabolism and/or protein synthesis will only utilize the L-am ...
... 2. Enzymes are Stereospecific. If a molecule exists as a pair of enantiomers, the enzyme will use only one of the pair as substrate and produce only one of the pair as the product. For example, the enzymes that are involved in amino acid metabolism and/or protein synthesis will only utilize the L-am ...
NCAM Polypeptides in Heart Development: Association with Z Discs
... microfuge (14,000 rpm for 3 rain). The membranes were extracted with NP40 as described above, and antigen-antibody complexes were collected by incubation with 50 ~1 of protein A-Sepharose for 1 h at 4°C. The beads were then washed several times with 0.5% NP-40/PBS. Bound NCAM was eluted by boiling t ...
... microfuge (14,000 rpm for 3 rain). The membranes were extracted with NP40 as described above, and antigen-antibody complexes were collected by incubation with 50 ~1 of protein A-Sepharose for 1 h at 4°C. The beads were then washed several times with 0.5% NP-40/PBS. Bound NCAM was eluted by boiling t ...
Amino Acids
... • Collagen superfamily of proteins include > 20 collagen types, as well as additional proteins that have collagenlike domains. • The 3 polyp. α-chains are held together by H-bonds b/w the chains. • Variations in aa sequence of α-chains result in structural components that are ~ the same size (~ 1000 ...
... • Collagen superfamily of proteins include > 20 collagen types, as well as additional proteins that have collagenlike domains. • The 3 polyp. α-chains are held together by H-bonds b/w the chains. • Variations in aa sequence of α-chains result in structural components that are ~ the same size (~ 1000 ...
Early bioenergetic evolution
... them makes no contact with magma and emerges at a temperature of around 70–908C. Unlike vent systems that reside directly on the spreading zone, off-ridge vents can be stably active over geological time; Lost City has been active for about 120 000 years [18]. The chemical disequilibrium at vents ari ...
... them makes no contact with magma and emerges at a temperature of around 70–908C. Unlike vent systems that reside directly on the spreading zone, off-ridge vents can be stably active over geological time; Lost City has been active for about 120 000 years [18]. The chemical disequilibrium at vents ari ...
Protein Synthesis in Cell-Free Reticulocyte Lysates on Multi
... are significant in comparison to the behavior of the unsupplemented translation mixtures. Even in the optimized translation mixture, however, the overall level of incorporation corresponds only to a level of protein synthesis of - 2 p"glmL. which is about 2o/oof the activity of the most active ...
... are significant in comparison to the behavior of the unsupplemented translation mixtures. Even in the optimized translation mixture, however, the overall level of incorporation corresponds only to a level of protein synthesis of - 2 p"glmL. which is about 2o/oof the activity of the most active ...
Conformational changes in actin–myosin isoforms probed by Ni(II
... Quantamaster PTI fluorimeter. Emission spectra were monitored at 25 °C from 450 nm to 600 nm with an excitation wavelength of 425 nm. SDS-PAGE and western blotting Gel electrophoresis was performed as described by Laemmli using a 4–18% gradient acrylamide gel and stained with Coomassie Blue. Densitom ...
... Quantamaster PTI fluorimeter. Emission spectra were monitored at 25 °C from 450 nm to 600 nm with an excitation wavelength of 425 nm. SDS-PAGE and western blotting Gel electrophoresis was performed as described by Laemmli using a 4–18% gradient acrylamide gel and stained with Coomassie Blue. Densitom ...
Bio 226: Cell and Molecular Biology
... Several Calvin cycle enzymes (e.g.Fructose-1,6bisphosphatase) are also regulated by thioredoxin contain disulfide bonds which get oxidized in the dark in light, ferredoxin reduces thioredoxin, thioredoxin reduces these disulfide bonds to activate the enzyme How light reactions talk to the Calvin cyc ...
... Several Calvin cycle enzymes (e.g.Fructose-1,6bisphosphatase) are also regulated by thioredoxin contain disulfide bonds which get oxidized in the dark in light, ferredoxin reduces thioredoxin, thioredoxin reduces these disulfide bonds to activate the enzyme How light reactions talk to the Calvin cyc ...
Descriptions of Banbury Conference
... xenopus model for fragile X, and the insights which this may offer in understanding the transport functions of FMRP. The next session focused on the metabotropic glutamate receptors (mGluRs). Ever since the original discovery by Bill Greenough’s group that mGluR activation triggers local synthesis o ...
... xenopus model for fragile X, and the insights which this may offer in understanding the transport functions of FMRP. The next session focused on the metabotropic glutamate receptors (mGluRs). Ever since the original discovery by Bill Greenough’s group that mGluR activation triggers local synthesis o ...
Isolation of Casein, Lactose and Albumin from Milk
... release a small segment of it. This destroys the water-solubilizing surface of the κ casein, which protects the inner α and β caseins and causes the entire micelle to precipitate as calcium paracaseinate. Milk can be decalcified by treatment with oxalate ion, which forms an insoluble calcium salt. I ...
... release a small segment of it. This destroys the water-solubilizing surface of the κ casein, which protects the inner α and β caseins and causes the entire micelle to precipitate as calcium paracaseinate. Milk can be decalcified by treatment with oxalate ion, which forms an insoluble calcium salt. I ...
A conserved N-‐terminal domain of Rif2 regulates telomere length in
... Telomere sequence DNA repeats are bound by a set of proteins that mediate two essential functions; first to protect the ends from degradation, recombination, and initiation of a damage response, and second ...
... Telomere sequence DNA repeats are bound by a set of proteins that mediate two essential functions; first to protect the ends from degradation, recombination, and initiation of a damage response, and second ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.