Regulation of intermediary metabolism by protein acetylation
... cellular components. These steps are catalyzed by enzymes within the cell and provide energy, reducing power and building blocks for maintaining cellular homeostasis or supporting cell growth. Protein lysine deacetylases: also known as histone deacetylases, HDACs. These comprise a group of hydrolase ...
... cellular components. These steps are catalyzed by enzymes within the cell and provide energy, reducing power and building blocks for maintaining cellular homeostasis or supporting cell growth. Protein lysine deacetylases: also known as histone deacetylases, HDACs. These comprise a group of hydrolase ...
Acyl-CoA oxidase is imported as a heteropentameric, cofactor
... Figure 2. In the peroxisomal matrix, the five Aox isoforms constitute a 443-kD heteropentameric complex. (A) Peroxisomes purified from the 20KgP fraction of YPBO-grown wild-type (P01d) cells were lysed by addition of LC buffer and subjected to centrifugation to yield a supernatant enriched for matri ...
... Figure 2. In the peroxisomal matrix, the five Aox isoforms constitute a 443-kD heteropentameric complex. (A) Peroxisomes purified from the 20KgP fraction of YPBO-grown wild-type (P01d) cells were lysed by addition of LC buffer and subjected to centrifugation to yield a supernatant enriched for matri ...
Recent Advances Towards New Anti-Infective Agents that Inhibit
... amino acid), a hydrophobic domain, and a tail of mostly positively charged residues. The charged amino acids at the C-terminal end of the CWS are believed to prevent the protein from being released into the extracellular milieu. (3) The partially exported protein is then processed by the SrtA sortas ...
... amino acid), a hydrophobic domain, and a tail of mostly positively charged residues. The charged amino acids at the C-terminal end of the CWS are believed to prevent the protein from being released into the extracellular milieu. (3) The partially exported protein is then processed by the SrtA sortas ...
... at pH 2.5 for 10 min to remove poorly bound proteins, blocked with 5% skimmed milk powder in TRIS-buffered saline–Tween (TBST) for 45 min, followed by overnight incubation with 10 ml of the antiserum, and subsequently washed thoroughly with TBST buffer. Purified antibodies were eluted with 100 mM gl ...
Abstract Background The present study aimed to compare the
... quantitation (iTRAQ) reagents (Applied Biosystems) according to the manufacturer's instructions. Samples from wild ginseng were labeled with reagent 114, and samples from cultivated ginseng were labeled with reagent 115. Two independent biological experiments with three technical repeats each were p ...
... quantitation (iTRAQ) reagents (Applied Biosystems) according to the manufacturer's instructions. Samples from wild ginseng were labeled with reagent 114, and samples from cultivated ginseng were labeled with reagent 115. Two independent biological experiments with three technical repeats each were p ...
thyroid synthesis, mode of action, metabolic functions and disorders
... • Each thyroglobulin molecule contains up to 30 thyroxine molecules and a few triiodothyronine molecules. • Stored Thyroid Hormones maintain the body’s requirement of T3 and T4 for up to 2-3 months Release of Thyroid Hormones • Thyroglobulin itself is not secreted into the circulation • Thyroglobuli ...
... • Each thyroglobulin molecule contains up to 30 thyroxine molecules and a few triiodothyronine molecules. • Stored Thyroid Hormones maintain the body’s requirement of T3 and T4 for up to 2-3 months Release of Thyroid Hormones • Thyroglobulin itself is not secreted into the circulation • Thyroglobuli ...
Cyclic-‐di-‐AMP: another second messenger enters the fray
... are found in bacterial species but a small number are also present in archaea within the Euryarchaeota group (Box 2)12. In bacteria, DAC domain proteins are most frequently found in the G ...
... are found in bacterial species but a small number are also present in archaea within the Euryarchaeota group (Box 2)12. In bacteria, DAC domain proteins are most frequently found in the G ...
16. enzymes i – nomenclature and classification
... pressures—the conditions under which living cells carry on their life processes. Yet in the living cells these reactions proceed at extremely high rates. This is due to the presence of some catalysts produced and synthesized inside the body of the organisms. The term ‘enzyme’ was In fact, Kuhne inte ...
... pressures—the conditions under which living cells carry on their life processes. Yet in the living cells these reactions proceed at extremely high rates. This is due to the presence of some catalysts produced and synthesized inside the body of the organisms. The term ‘enzyme’ was In fact, Kuhne inte ...
Immobilized Enzyme Technology: Potentiality and Prospects
... Crosslinking It is an irreversible method of enzyme immobilization (Figure 1g). It is different from other techniques in the sense that it does not require a support for the immobilization. There are two methods of cross linking in use, (i) Cross Linking Enzyme Aggregate (CLEA), and (ii) Cross Linki ...
... Crosslinking It is an irreversible method of enzyme immobilization (Figure 1g). It is different from other techniques in the sense that it does not require a support for the immobilization. There are two methods of cross linking in use, (i) Cross Linking Enzyme Aggregate (CLEA), and (ii) Cross Linki ...
IN VIVO ENOL CASTOR OIL SEEDS AT THREONINE-4 AND SERINE-451
... everyday and a lot of nights with laughs. I would like to specially thank Brendan O’Leary for all his guidance and support in and out of the lab, the journey would not have been as enjoyable without such a great mentor in the lab. Thank you to the Snedden lab, Kyle Bender and David Maj, for always p ...
... everyday and a lot of nights with laughs. I would like to specially thank Brendan O’Leary for all his guidance and support in and out of the lab, the journey would not have been as enjoyable without such a great mentor in the lab. Thank you to the Snedden lab, Kyle Bender and David Maj, for always p ...
Developmental changes in barley microRNA expression profiles
... small RNA libraries were designed for each barley developmental stage. Three replicas were performed using an Illumina TruSeq Small RNA Library Preparation Kit (Illumina). The quantification of libraries was carried out using a Quant-iT PicoGreen dsDNA Assay kit (Molecular Probes) reagent and Tecan ...
... small RNA libraries were designed for each barley developmental stage. Three replicas were performed using an Illumina TruSeq Small RNA Library Preparation Kit (Illumina). The quantification of libraries was carried out using a Quant-iT PicoGreen dsDNA Assay kit (Molecular Probes) reagent and Tecan ...
In situ hybridization
... hybridizing the complementary strand of a nucleotide probe to the sequence of interest. Normal hybridization requires the isolation of DNA or RNA, separating it on a gel, blotting it onto nitrocellulose and probing it with a complementary sequence. The basic principles for in situ hybridization are ...
... hybridizing the complementary strand of a nucleotide probe to the sequence of interest. Normal hybridization requires the isolation of DNA or RNA, separating it on a gel, blotting it onto nitrocellulose and probing it with a complementary sequence. The basic principles for in situ hybridization are ...
Golgins and GTPases, giving identity and structure to the Golgi
... Fig. 1. Golgins associate with Golgi membranes in a variety of ways. Golgins are a diverse family of Golgi-localized coiled-coil proteins and interact with Golgi membranes in a variety of different ways. (a) Some have a transmembrane domain near their C-terminus while others are peripheral membrane ...
... Fig. 1. Golgins associate with Golgi membranes in a variety of ways. Golgins are a diverse family of Golgi-localized coiled-coil proteins and interact with Golgi membranes in a variety of different ways. (a) Some have a transmembrane domain near their C-terminus while others are peripheral membrane ...
Marine Biotecnology
... To identify the pure enzyme, 3 μg PVL were electrophoretically separated on a 12% acrylamide gel. After separation, the gel was stained with Coomassie Blue to identify protein bands that were cut from the gel. The sequence analysis was performed at the Wistar Institute Proteomics Facility using a tr ...
... To identify the pure enzyme, 3 μg PVL were electrophoretically separated on a 12% acrylamide gel. After separation, the gel was stained with Coomassie Blue to identify protein bands that were cut from the gel. The sequence analysis was performed at the Wistar Institute Proteomics Facility using a tr ...
Purification and Biochemical Characterization of Digestive Lipase in
... To identify the pure enzyme, 3 μg PVL were electrophoretically separated on a 12% acrylamide gel. After separation, the gel was stained with Coomassie Blue to identify protein bands that were cut from the gel. The sequence analysis was performed at the Wistar Institute Proteomics Facility using a tr ...
... To identify the pure enzyme, 3 μg PVL were electrophoretically separated on a 12% acrylamide gel. After separation, the gel was stained with Coomassie Blue to identify protein bands that were cut from the gel. The sequence analysis was performed at the Wistar Institute Proteomics Facility using a tr ...
Detection and Estimation of Collagen
... In 1970 Mitchell and Taylor13 critically examined the available methods and concluded that no single method could combine accuracy with freedom from errors caused by interferring substances. Consequently, several different experimental approaches can be found in the literature, depending upon the na ...
... In 1970 Mitchell and Taylor13 critically examined the available methods and concluded that no single method could combine accuracy with freedom from errors caused by interferring substances. Consequently, several different experimental approaches can be found in the literature, depending upon the na ...
PDF - Oxford Academic - Oxford University Press
... SDPpred does not require information about the 3D protein structure, which instead can be used at the post-processing step to validate the predictions. There are also other approaches for comparative analysis of protein subfamilies. One of these methods, called DIVERGE (17) takes into account the di ...
... SDPpred does not require information about the 3D protein structure, which instead can be used at the post-processing step to validate the predictions. There are also other approaches for comparative analysis of protein subfamilies. One of these methods, called DIVERGE (17) takes into account the di ...
Staphylococcus haemolyticus lipase
... experiment, neither prepro- nor proenzyme bands could be detected by Coomassie staining or activity staining of the SDS-PAGE gel. The optimum temperature for the lipase L62 was determined to be 28³C, however, it showed as much as 30% activity at 4³C (Fig. 2). This high activity at low temperature ma ...
... experiment, neither prepro- nor proenzyme bands could be detected by Coomassie staining or activity staining of the SDS-PAGE gel. The optimum temperature for the lipase L62 was determined to be 28³C, however, it showed as much as 30% activity at 4³C (Fig. 2). This high activity at low temperature ma ...
Protein translation in Plasmodium parasites
... encoded by the nuclear genome, translated in the cytoplasm, and transported into the apicoplast using specific targeting signals [4]. Detection of plastid-encoded EF-Tu by western blot and immunofluorescence has provided evidence for translation in P. falciparum plastids [5]. Indirect evidence of ap ...
... encoded by the nuclear genome, translated in the cytoplasm, and transported into the apicoplast using specific targeting signals [4]. Detection of plastid-encoded EF-Tu by western blot and immunofluorescence has provided evidence for translation in P. falciparum plastids [5]. Indirect evidence of ap ...
Growth, digestive and absorptive capacity and
... digestive capacity in protein and fat [6, 8]. Therefore, effects of dietary threonine on digestive and absorptive capacity may be different among fish with different feeding habits. The present study focused on the effects of threonine on digestive and absorptive capacity of herbivorous grass carp ( ...
... digestive capacity in protein and fat [6, 8]. Therefore, effects of dietary threonine on digestive and absorptive capacity may be different among fish with different feeding habits. The present study focused on the effects of threonine on digestive and absorptive capacity of herbivorous grass carp ( ...
Bioactivation of Selenocysteine Se-Conjugates by a Highly Purified
... and yielded a highly purified enzyme that was 1000-fold enriched according to its increased specific activity with S-(1,2-dichlorovinyl)L-cysteine (1,2-DCV-Cys) as a substrate. Matrix-assisted laser desorption time-of-flight (MALDI-TOF) mass spectrometry analysis of this protein revealed a single MH ...
... and yielded a highly purified enzyme that was 1000-fold enriched according to its increased specific activity with S-(1,2-dichlorovinyl)L-cysteine (1,2-DCV-Cys) as a substrate. Matrix-assisted laser desorption time-of-flight (MALDI-TOF) mass spectrometry analysis of this protein revealed a single MH ...
Drosophila Sirt2/mammalian SIRT3 deacetylates ATP synthase and
... Figure 1. Increase in ceramide levels results in depletion of NAD+ and decrease in sirtuin activity leading to hyperacetylation of proteins in different cellular compartments. (A) dcerk1 fly extracts show 65% reduction in NAD+ level compared with w1118 control. n = 3. (B) NAD synthesis and salvage ...
... Figure 1. Increase in ceramide levels results in depletion of NAD+ and decrease in sirtuin activity leading to hyperacetylation of proteins in different cellular compartments. (A) dcerk1 fly extracts show 65% reduction in NAD+ level compared with w1118 control. n = 3. (B) NAD synthesis and salvage ...
embor2011116-sup-0001
... in the aggregation rate/propensity of a given protein following a given mutation, with this parameter estimated repeatedly using a number of single or multiple mutations. Our review aimed at evaluating the correlation between such experimental data and those estimated in silico by several algorithm ...
... in the aggregation rate/propensity of a given protein following a given mutation, with this parameter estimated repeatedly using a number of single or multiple mutations. Our review aimed at evaluating the correlation between such experimental data and those estimated in silico by several algorithm ...
Stringent Response in Myxococcus xanthus
... How do we test to see if DK527is really a relA- mutant? ...
... How do we test to see if DK527is really a relA- mutant? ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.