![Hydrolysis of a Series of Synthetic Peptide Substrates by the Human](http://s1.studyres.com/store/data/004457271_1-b026f59ca0e72562a36add85d6c775ec-300x300.png)
Hydrolysis of a Series of Synthetic Peptide Substrates by the Human
... picornaviruses, including human rhinoviruses. However there have been relatively few comprehensive studies on the rhinovirus 3C protein (Libby et al., 1988). To ascertain not only whether this protein expressed similar proteolytic activity but also whether it is a suitable target for antiviral chemo ...
... picornaviruses, including human rhinoviruses. However there have been relatively few comprehensive studies on the rhinovirus 3C protein (Libby et al., 1988). To ascertain not only whether this protein expressed similar proteolytic activity but also whether it is a suitable target for antiviral chemo ...
Glycation by Ascorbic Acid Causes Loss of Activity of Ribulose
... Fig. 3 SDS-PAGE profile of glycated Rubisco. Purified Rubisco was incubated with 10 mM AsA or [14C]AsA at 25°C for indicated days. Native Rubisco is represented as 0 day Rubisco. After the solution was dialyzed extensively against distilled water, an equal volume of SDSPAGE sample buffer was added a ...
... Fig. 3 SDS-PAGE profile of glycated Rubisco. Purified Rubisco was incubated with 10 mM AsA or [14C]AsA at 25°C for indicated days. Native Rubisco is represented as 0 day Rubisco. After the solution was dialyzed extensively against distilled water, an equal volume of SDSPAGE sample buffer was added a ...
The relevance of carbon dioxide metabolism in
... proteomic analysis, washed in NaCl solution to reach a final density of 1.2 (OD600), and inoculated (5 %, v/v) in reconstituted skimmed milk (Difco Laboratories) that had been pasteurized at 80 uC for 10 min. Acidification measurements were followed by using a standard pH meter for 24 h at 37 uC. Th ...
... proteomic analysis, washed in NaCl solution to reach a final density of 1.2 (OD600), and inoculated (5 %, v/v) in reconstituted skimmed milk (Difco Laboratories) that had been pasteurized at 80 uC for 10 min. Acidification measurements were followed by using a standard pH meter for 24 h at 37 uC. Th ...
Antioxidant Enzymes and Function
... same assay as GPx, measure NADPH Other substrates besides GSSG: only mixed disulfides between GSH & γ-glutamylcysteine or CoA ...
... same assay as GPx, measure NADPH Other substrates besides GSSG: only mixed disulfides between GSH & γ-glutamylcysteine or CoA ...
Energy Calculations for 4-Hydroxy-3
... Part V: Protein-Ligand Interactions In Part V of the Protein Modeling Assignment, the wiring diagram of the protein, Isopentenyl-Diphosphate Delta-Isomerase, was obtained from the PDBSum website. The wiring diagram displays all the amino acids in one subunit of the protein and indicates, with red do ...
... Part V: Protein-Ligand Interactions In Part V of the Protein Modeling Assignment, the wiring diagram of the protein, Isopentenyl-Diphosphate Delta-Isomerase, was obtained from the PDBSum website. The wiring diagram displays all the amino acids in one subunit of the protein and indicates, with red do ...
Description of controlled spike
... Twelve endogenous peptides from six proteins were mixed at varying concentrations in a Latin Square design (Figure 1a). These peptides serve as positive controls, and represent proteins with true changes in concentration across mixtures. The second set of twelve endogenous peptides from the remainin ...
... Twelve endogenous peptides from six proteins were mixed at varying concentrations in a Latin Square design (Figure 1a). These peptides serve as positive controls, and represent proteins with true changes in concentration across mixtures. The second set of twelve endogenous peptides from the remainin ...
9 The AMP-activated protein kinase: more than an energy sensor
... At any time, intracellular ATP concentrations reflect the balance between energy supply-and-demand. In most cells, ATP supply relies on mitochondrial oxidative phosphorylation, whereas ATP demand depends on the energy required to perform various cell functions. Any decrease in supply (e.g. hypoxia) ...
... At any time, intracellular ATP concentrations reflect the balance between energy supply-and-demand. In most cells, ATP supply relies on mitochondrial oxidative phosphorylation, whereas ATP demand depends on the energy required to perform various cell functions. Any decrease in supply (e.g. hypoxia) ...
Full-Text PDF
... biopanning, the Sal-conjugated T7 phage-displayed peptides showed the strongest binding to streptavidin (Figure 6B), suggesting that the Sal moiety of the peptides played a crucial role in interaction with the target. Eight of the T7 phage monoclones were randomly chosen from the phage pool. Three o ...
... biopanning, the Sal-conjugated T7 phage-displayed peptides showed the strongest binding to streptavidin (Figure 6B), suggesting that the Sal moiety of the peptides played a crucial role in interaction with the target. Eight of the T7 phage monoclones were randomly chosen from the phage pool. Three o ...
Enhancement of the Essential Amino Acid Composition of Food
... fragments [16] were conducted according to the methods of Display Systems Biotech, Vista, CA, USA except that 15 µL instead of 3 µL of the amplified reaction solution was loaded into the sequencing gel. All the 64 Display Probes were applied for the restriction fragment PCR amplifications. Selected ...
... fragments [16] were conducted according to the methods of Display Systems Biotech, Vista, CA, USA except that 15 µL instead of 3 µL of the amplified reaction solution was loaded into the sequencing gel. All the 64 Display Probes were applied for the restriction fragment PCR amplifications. Selected ...
Investigation of Factors Affecting Opalescence and Phase
... antibody-based therapeutics such as ADCs, bispecific antibodies, engineered antibodies and antibody fragments or domains have generated quite an interest over last few years. These newer molecules have further enhanced the therapeutic efficacy of the first generation of antibodies and have vastly im ...
... antibody-based therapeutics such as ADCs, bispecific antibodies, engineered antibodies and antibody fragments or domains have generated quite an interest over last few years. These newer molecules have further enhanced the therapeutic efficacy of the first generation of antibodies and have vastly im ...
The sequence of human serum albumin cDNA and its expression in
... expression of the mature protein in £ . c o l i via the trp promoter-operator. The plasmid pLelF A25 directs the expression of human leukocyte interferon A (IFNa2) (21). I t was digested with Xbal and the cleavage site " f i l l e d i n " to produce blunt DNA ends with DNA polymerase I Klenow fragme ...
... expression of the mature protein in £ . c o l i via the trp promoter-operator. The plasmid pLelF A25 directs the expression of human leukocyte interferon A (IFNa2) (21). I t was digested with Xbal and the cleavage site " f i l l e d i n " to produce blunt DNA ends with DNA polymerase I Klenow fragme ...
Effect of Ca on H -ATPase activity of plasma membrane in
... Zhao Hu-Cheng *, Zhu Ting, Wu Jia, Xi Bao-Shu Biomechanics Lab, Department of Engineering Mechanics, Tsinghua University, Beijing 100084, People’s Republic of China ...
... Zhao Hu-Cheng *, Zhu Ting, Wu Jia, Xi Bao-Shu Biomechanics Lab, Department of Engineering Mechanics, Tsinghua University, Beijing 100084, People’s Republic of China ...
informed bodybuilding nutrition
... eliminates bias. In the case of bodybuilding, subjects would be on the same training regime and diet plan for a set period, when the results are compared, the study designers will look for statistical significance between the two groups. If, present this strongly suggests that the substance in quest ...
... eliminates bias. In the case of bodybuilding, subjects would be on the same training regime and diet plan for a set period, when the results are compared, the study designers will look for statistical significance between the two groups. If, present this strongly suggests that the substance in quest ...
Raw Glycerol as Substrate for the Production of Yeast Biomass
... C. lipolytica NRRL Y-1095 showed the highest values (1.9 0.1 g/L and 1.8 0.1 g/L, respectively) for analytical grade glycerol, differing statistically from the other yeast strains. In the medium containing glycerol derived from biodiesel production, there was no statistically significant differe ...
... C. lipolytica NRRL Y-1095 showed the highest values (1.9 0.1 g/L and 1.8 0.1 g/L, respectively) for analytical grade glycerol, differing statistically from the other yeast strains. In the medium containing glycerol derived from biodiesel production, there was no statistically significant differe ...
Chapter 9 Slides
... (organic solvents, or strong detergents) • Often transmembrane but not necessarily • Glycophorin, bacteriorhodopsin are examples Copyright © 1999 by Harcourt Brace & Company ...
... (organic solvents, or strong detergents) • Often transmembrane but not necessarily • Glycophorin, bacteriorhodopsin are examples Copyright © 1999 by Harcourt Brace & Company ...
The Role of Bcl-2 in Apoptosis and Cancer
... 2. Knockout mice further demonstrate the link between Bcl-2 and apoptosis. -Bcl-2 mutants undergo massive apoptosis inside certain organs and do not properly execute melanin synthesis (gray mice). -If even one allele of a primary BH3-only protein is lost as well, the Bcl-2 mutant mouse is rescued. 3 ...
... 2. Knockout mice further demonstrate the link between Bcl-2 and apoptosis. -Bcl-2 mutants undergo massive apoptosis inside certain organs and do not properly execute melanin synthesis (gray mice). -If even one allele of a primary BH3-only protein is lost as well, the Bcl-2 mutant mouse is rescued. 3 ...
Cell and Molecular Biology
... You have isolated an antibiotic named edeine, from a bacterial culture. Edeine inhibits protein synthesis but has not effect on either DNA synthesis or RNA synthesis. When added to a reticulocyte lysate, edeine stops protein synthesis after a short lag, as shown below. By contrast, cycloheximide st ...
... You have isolated an antibiotic named edeine, from a bacterial culture. Edeine inhibits protein synthesis but has not effect on either DNA synthesis or RNA synthesis. When added to a reticulocyte lysate, edeine stops protein synthesis after a short lag, as shown below. By contrast, cycloheximide st ...
Biosensors
... ii. They can be excited by electron beams or by light. The most useful are non-radiative plasmons excited by evanescent light waves. The plasmon is characterized by an exponential decrease in the electric filed with increasing distance from the boundary. iii. The p-polarized incident filed, which ha ...
... ii. They can be excited by electron beams or by light. The most useful are non-radiative plasmons excited by evanescent light waves. The plasmon is characterized by an exponential decrease in the electric filed with increasing distance from the boundary. iii. The p-polarized incident filed, which ha ...
BIF CH4 5th proofs.qxd
... function if one of the proteins is known only from its sequence. The operation of natural selection tends to result in the acceptance of mutations that preserve the folding and function of a protein, whereas those that destroy folding or function will be eliminated. However, similar or identical ali ...
... function if one of the proteins is known only from its sequence. The operation of natural selection tends to result in the acceptance of mutations that preserve the folding and function of a protein, whereas those that destroy folding or function will be eliminated. However, similar or identical ali ...
Host Factors in the Replication of Positive
... Accumulating evidence suggests that the entire RNA replication process of (+)RNA viruses is complex, involving not only interactions of viral RNAs and viral proteins but also their interplay with host factors. Studies performed for infections with HCV, DENV, WNV, and several plant (+)RNA viruses hav ...
... Accumulating evidence suggests that the entire RNA replication process of (+)RNA viruses is complex, involving not only interactions of viral RNAs and viral proteins but also their interplay with host factors. Studies performed for infections with HCV, DENV, WNV, and several plant (+)RNA viruses hav ...
feeding for milk composition
... pH and milk fat for diets adjusted for a common nonforage NDF basis. Contrary to Erdman (1988) and Armentano and Pereira (1997), Allen (1997) observed a linear relationship between mean rumen pH and milk fat content (r2 = 0.39) for 90 observations from 23 studies reported in the literature. Regardle ...
... pH and milk fat for diets adjusted for a common nonforage NDF basis. Contrary to Erdman (1988) and Armentano and Pereira (1997), Allen (1997) observed a linear relationship between mean rumen pH and milk fat content (r2 = 0.39) for 90 observations from 23 studies reported in the literature. Regardle ...
Yeast lipid metabolism at a glance
... serve as an energy reservoir in triacylglycerols (TG) and steryl esters (SE) stored in LD. FA can also function as transcriptional regulators and signaling molecules and can be involved in post-translational modification of proteins. Free fatty acids (FFA) are carboxylic acids with hydrocarbon tails ...
... serve as an energy reservoir in triacylglycerols (TG) and steryl esters (SE) stored in LD. FA can also function as transcriptional regulators and signaling molecules and can be involved in post-translational modification of proteins. Free fatty acids (FFA) are carboxylic acids with hydrocarbon tails ...
MD1-103 Protein Stability Combo Kit
... DSF takes advantage of the fact that the fluorescence of many nonspecific protein-binding dyes (e.g. SYPRO Orange) increases together with increasing hydrophobicity of their environment. In principle, the protein solution is heated in the presence of SYPRO Orange. Upon denaturation, the dye binds to ...
... DSF takes advantage of the fact that the fluorescence of many nonspecific protein-binding dyes (e.g. SYPRO Orange) increases together with increasing hydrophobicity of their environment. In principle, the protein solution is heated in the presence of SYPRO Orange. Upon denaturation, the dye binds to ...
Western blot
![](https://commons.wikimedia.org/wiki/Special:FilePath/Anti-lipoic_acid_immunoblot.png?width=300)
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.