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Fluorescence Enhancement of Fluorescein Isothiocyanate
... binding was 1.6-fold with protein A ― IgG interaction in PBS [8] and 1.5-fold with DNA interaction [7]. On the other hand, glycerin treatment enhances fluorescence of immobilized FITC-labeled antibody by approximately 1.5-fold [9]. The lower fluorescence enhancement shown in Figure 3, which may be c ...
... binding was 1.6-fold with protein A ― IgG interaction in PBS [8] and 1.5-fold with DNA interaction [7]. On the other hand, glycerin treatment enhances fluorescence of immobilized FITC-labeled antibody by approximately 1.5-fold [9]. The lower fluorescence enhancement shown in Figure 3, which may be c ...
HEMOSTASIS
... receptor for vWF, which serves to mediate the initial adhesion of platelets to subendothelium. ...
... receptor for vWF, which serves to mediate the initial adhesion of platelets to subendothelium. ...
Fastness in Mycobacterium tuberculosis
... vivo phosphorylation event in Mtb was gained using a mass spectrometry-based approach to identify phosphorylation sites in Mtb proteins [4]. This provided insights into the range of functions regulated by Ser/Thr phosphorylation, underpinning the involvement of many STPK in regulating metabolic proc ...
... vivo phosphorylation event in Mtb was gained using a mass spectrometry-based approach to identify phosphorylation sites in Mtb proteins [4]. This provided insights into the range of functions regulated by Ser/Thr phosphorylation, underpinning the involvement of many STPK in regulating metabolic proc ...
Lec 16: Nitrogen (ammonia) assimilation
... On the other hand, this reaction occurs in the αKG formation direction for animals because intracellular concentration of NH3 is expected to be low… as NH3 is toxic to animal. (Animals do not utilize NH3 directly as nitrogen source!) ...
... On the other hand, this reaction occurs in the αKG formation direction for animals because intracellular concentration of NH3 is expected to be low… as NH3 is toxic to animal. (Animals do not utilize NH3 directly as nitrogen source!) ...
Structure, function, and evolution of phosphoglycerate mutases
... their structural and functional similarity as well as a common evolutionary ancestor (Grana et al., 1995). They also have been identi®ed to have some sequence similarities with alkaline phosphatases but only over a limited part of the active site residues responsible for metal binding (Galperin et a ...
... their structural and functional similarity as well as a common evolutionary ancestor (Grana et al., 1995). They also have been identi®ed to have some sequence similarities with alkaline phosphatases but only over a limited part of the active site residues responsible for metal binding (Galperin et a ...
attachment 1 - Federal Register of Legislation
... MIR604 in Australia and New Zealand is agreed on the basis of the available scientific evidence for the following reasons: ...
... MIR604 in Australia and New Zealand is agreed on the basis of the available scientific evidence for the following reasons: ...
Full-Text PDF
... oxidation states of +VI (tellurate), +IV (tellurite), 0 (elemental Te), and −II (telluride). The majority is found in the hydrosphere as tellurate and in the lithosphere as tellurides of gold and silver [1]. The most harmful forms of Te to microorganisms are the oxyanions, especially tellurite, with ...
... oxidation states of +VI (tellurate), +IV (tellurite), 0 (elemental Te), and −II (telluride). The majority is found in the hydrosphere as tellurate and in the lithosphere as tellurides of gold and silver [1]. The most harmful forms of Te to microorganisms are the oxyanions, especially tellurite, with ...
Isolation of Casein, Lactose, and Albumin from Milk
... to it. It is also thought to have all its serine and threonine residues (which have hydroxyl groups), as well as its bound carbohydrates, on only one side of its outer surfaces. This portion of its outer surface is easily solubilized in water since these polar groups are present. The other portion o ...
... to it. It is also thought to have all its serine and threonine residues (which have hydroxyl groups), as well as its bound carbohydrates, on only one side of its outer surfaces. This portion of its outer surface is easily solubilized in water since these polar groups are present. The other portion o ...
Purification and Characterization of Two Thermostable Proteases
... a dialysis sack overnight against three changes of the same buffer. Insoluble material was removed by centrifugation at 10,000 ×g for 10 min. (2) Ion-exchange chromatography on a DEAE-Sepharose column (1×20 cm) equilibrated with buffer A. After the column had been washed with five column volumes of ...
... a dialysis sack overnight against three changes of the same buffer. Insoluble material was removed by centrifugation at 10,000 ×g for 10 min. (2) Ion-exchange chromatography on a DEAE-Sepharose column (1×20 cm) equilibrated with buffer A. After the column had been washed with five column volumes of ...
02_whole - Massey Research Online
... tissue, MD-AC02 (in common with MD-A CO]) is expressed predominantly in mature fruit tissue. None of the MD-A COs were observed to be senescence associated genes (SAG). MD AC03 protein accumulated predominantly in young leaf tissue and less intensely in both mature leaf tissue and young fruit tissu ...
... tissue, MD-AC02 (in common with MD-A CO]) is expressed predominantly in mature fruit tissue. None of the MD-A COs were observed to be senescence associated genes (SAG). MD AC03 protein accumulated predominantly in young leaf tissue and less intensely in both mature leaf tissue and young fruit tissu ...
and Rhizobiales-Like PPP-Family Protein Phosphatases from
... Reversible protein phosphorylation catalyzed by protein kinases and phosphatases represents the most prolific and currently best-characterized post-translational modification. Over the last decade advancements in genome sequencing technologies has massively increased genomic databases, resulting in ...
... Reversible protein phosphorylation catalyzed by protein kinases and phosphatases represents the most prolific and currently best-characterized post-translational modification. Over the last decade advancements in genome sequencing technologies has massively increased genomic databases, resulting in ...
Hücrelerin Yapısı - mustafaaltinisik.org.uk
... (organic solvents, or strong detergents) • Often transmembrane but not necessarily • Glycophorin, bacteriorhodopsin are examples Copyright © 1999 by Harcourt Brace & Company ...
... (organic solvents, or strong detergents) • Often transmembrane but not necessarily • Glycophorin, bacteriorhodopsin are examples Copyright © 1999 by Harcourt Brace & Company ...
cell biology - Bio
... Cell Biology, A Short Course aims to cover a wide area of cell biology in a form especially suitable for first year undergraduates. We have deliberately kept the book to a manageable size so that neither the cost, the content, nor the weight is too daunting for the student. The overall theme for the ...
... Cell Biology, A Short Course aims to cover a wide area of cell biology in a form especially suitable for first year undergraduates. We have deliberately kept the book to a manageable size so that neither the cost, the content, nor the weight is too daunting for the student. The overall theme for the ...
Glycolytic strategy as a tradeoff between energy yield and protein cost
... phosphorylated twice and cleaved into two triose-phosphates (G3P and dihydroxyacetone phosphate), both of which are used to produce ATP through substrate-level phosphorylation in lower glycolysis (2, 7) (Fig. 1B). In the ED pathway, glucose is phosphorylated only once and oxidized to 2-keto-3-deoxy- ...
... phosphorylated twice and cleaved into two triose-phosphates (G3P and dihydroxyacetone phosphate), both of which are used to produce ATP through substrate-level phosphorylation in lower glycolysis (2, 7) (Fig. 1B). In the ED pathway, glucose is phosphorylated only once and oxidized to 2-keto-3-deoxy- ...
Cholecystokinin in White Sea Bream: Molecular Cloning, Regional
... pepstatin A (10 mg?mL21 each) and 100 mM Na3VO4. After protein determination using the Bio-Rad protein assay kit (BioRad, Richmond, CA, USA), aliquots of whole cell protein extract (30 mg/well) were denatured and separated by electrophoresis on 12% SDS polyacrylamide gel. The proteins were blotted o ...
... pepstatin A (10 mg?mL21 each) and 100 mM Na3VO4. After protein determination using the Bio-Rad protein assay kit (BioRad, Richmond, CA, USA), aliquots of whole cell protein extract (30 mg/well) were denatured and separated by electrophoresis on 12% SDS polyacrylamide gel. The proteins were blotted o ...
Production of Monoclonal Antibody Against Listeria monocytogenes
... fish (20 samples), and processed-meat products (20 samples) were collected from supermarkets and traditional markets located in Chinju City (Gyeongnam Province, Korea), and 10 g of each sample aseptically transferred into a stomacher bag with a filter lining. The meat and processed-meat samples were ...
... fish (20 samples), and processed-meat products (20 samples) were collected from supermarkets and traditional markets located in Chinju City (Gyeongnam Province, Korea), and 10 g of each sample aseptically transferred into a stomacher bag with a filter lining. The meat and processed-meat samples were ...
23. electron transport and oxidative phosphorylation
... The mitochondrial inner membrane contains the ATPsynthesizing enzyme complex called ATP synthetase or Fo The subscript of Fo is not zero but the letter O which denotes that it is F1 ATPase (Figs. 23–5 and 23-6). This enzyme complex has the portion of the ATP synthetase 2 major components F 0 and F 1 ...
... The mitochondrial inner membrane contains the ATPsynthesizing enzyme complex called ATP synthetase or Fo The subscript of Fo is not zero but the letter O which denotes that it is F1 ATPase (Figs. 23–5 and 23-6). This enzyme complex has the portion of the ATP synthetase 2 major components F 0 and F 1 ...
Recycling of vitamin B12 and NAD+ within the Pdu
... that PduS is a monomer and each monomer of PduS contains one non-covalently bound FMN and two [4Fe-4S] clusters which are oxygen-labile. Genetic studies showed that a pduS deletion decreased the growth rate of Salmonella on 1,2-PD supporting a role in cobalamin reduction in vivo. Further SDS-PAGE an ...
... that PduS is a monomer and each monomer of PduS contains one non-covalently bound FMN and two [4Fe-4S] clusters which are oxygen-labile. Genetic studies showed that a pduS deletion decreased the growth rate of Salmonella on 1,2-PD supporting a role in cobalamin reduction in vivo. Further SDS-PAGE an ...
Analysis of Nutrient Composition of Feather Meal and Feather Meal
... fatty acid content, minerals, and amino acids (total protein basis and borate-phosphate buffer insoluble). Another practical concern is the extent of variability in the feed product among plants and within a plant over time. For a nutritionist to feel comfortable using an ingredient, an accurate and ...
... fatty acid content, minerals, and amino acids (total protein basis and borate-phosphate buffer insoluble). Another practical concern is the extent of variability in the feed product among plants and within a plant over time. For a nutritionist to feel comfortable using an ingredient, an accurate and ...
Marine alga Sargassum horneri active component
... an important role in basic biological functions, such as intracellular clearance of defective proteins and organelles, differentiation, and development [3-5]. Dysfunctions in autophagy are associated with severe diseases, such as heart disease, neurodegenerative disorders, and cancers [4,6,7]. There ...
... an important role in basic biological functions, such as intracellular clearance of defective proteins and organelles, differentiation, and development [3-5]. Dysfunctions in autophagy are associated with severe diseases, such as heart disease, neurodegenerative disorders, and cancers [4,6,7]. There ...
Thiele et al.: `Genome-scale reconstruction of E. coli`s transcriptional
... Sulfate is the only ionic compound associated with it PDBID: 2D1P. mnmA aka TrmU: monomer based on molecular weight [30] (in the paper they carry out the characterization and purification): crystal (no structure; one crystal contained 1 mnmA-tRNA complex) [29]. The thioredoxin system had to be added ...
... Sulfate is the only ionic compound associated with it PDBID: 2D1P. mnmA aka TrmU: monomer based on molecular weight [30] (in the paper they carry out the characterization and purification): crystal (no structure; one crystal contained 1 mnmA-tRNA complex) [29]. The thioredoxin system had to be added ...
- Wiley Online Library
... strongly upregulated immediately upon oxygen limitation-induced termination of growth. Then the level declines but stays 3.5-fold above the baseline level seen during aerobic growth. The strong induction ...
... strongly upregulated immediately upon oxygen limitation-induced termination of growth. Then the level declines but stays 3.5-fold above the baseline level seen during aerobic growth. The strong induction ...
Single Processing Center Models for Human Dicer and Bacterial
... 110ab, and the quadruple mutant were found to be completely inactive, while other double mutants mimicked precisely the activity of the single amino acid mutants from which they were derived; identical results were obtained for both the 70 bp (Supplemental Figure S1 available on Cell website) and th ...
... 110ab, and the quadruple mutant were found to be completely inactive, while other double mutants mimicked precisely the activity of the single amino acid mutants from which they were derived; identical results were obtained for both the 70 bp (Supplemental Figure S1 available on Cell website) and th ...
The relevance of carbon dioxide metabolism in
... proteomic analysis, washed in NaCl solution to reach a final density of 1.2 (OD600), and inoculated (5 %, v/v) in reconstituted skimmed milk (Difco Laboratories) that had been pasteurized at 80 uC for 10 min. Acidification measurements were followed by using a standard pH meter for 24 h at 37 uC. Th ...
... proteomic analysis, washed in NaCl solution to reach a final density of 1.2 (OD600), and inoculated (5 %, v/v) in reconstituted skimmed milk (Difco Laboratories) that had been pasteurized at 80 uC for 10 min. Acidification measurements were followed by using a standard pH meter for 24 h at 37 uC. Th ...
Western blot
![](https://commons.wikimedia.org/wiki/Special:FilePath/Anti-lipoic_acid_immunoblot.png?width=300)
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.