The nonenzymatic subunit of pseutarin C, a
... the peptide sequences12 (sense primers KIVYRE [IP1: 36-41]; WEYFIA [IP2: 323-328]; RPYSIYV [IP3: 414-420]; AAKTTF [N-ter: 821-826] and antisense primers RPYSLHA [A2R: 877-883]; FPAING [A3R: 10151020]). The RT-PCR conditions were as follows: reverse transcription at 50°C for 30 minutes; followed by i ...
... the peptide sequences12 (sense primers KIVYRE [IP1: 36-41]; WEYFIA [IP2: 323-328]; RPYSIYV [IP3: 414-420]; AAKTTF [N-ter: 821-826] and antisense primers RPYSLHA [A2R: 877-883]; FPAING [A3R: 10151020]). The RT-PCR conditions were as follows: reverse transcription at 50°C for 30 minutes; followed by i ...
Planta - University of Regina
... For 3¢-RACE, total RNA was reverse-transcribed using the adapter primer (5¢-¢GGC CAC GCG TCG ACT AC (T)17-3¢). The PCR program was: 1 min of denaturation at 94 °C, 30 cycles of 30 s at 94 °C, 30 s at the selected annealing temperature (Ta, 42± 64 °C), 1 min at 72 °C, and 10 min of ®nal extension at ...
... For 3¢-RACE, total RNA was reverse-transcribed using the adapter primer (5¢-¢GGC CAC GCG TCG ACT AC (T)17-3¢). The PCR program was: 1 min of denaturation at 94 °C, 30 cycles of 30 s at 94 °C, 30 s at the selected annealing temperature (Ta, 42± 64 °C), 1 min at 72 °C, and 10 min of ®nal extension at ...
Book Review Werner Callebaut and Diego Rasskin
... (Downing et al. (2001)). Modular systems are not limited to biology. In fact, in most areas of human pursuit we find the mark of modular thinking. Its success lies on allowing a complex system or task to be broken down into smaller, simpler functions. The individual modules can be modified or operat ...
... (Downing et al. (2001)). Modular systems are not limited to biology. In fact, in most areas of human pursuit we find the mark of modular thinking. Its success lies on allowing a complex system or task to be broken down into smaller, simpler functions. The individual modules can be modified or operat ...
BioFiles v5 n1 - Sigma
... are necessary for proper mammalian development. Because sialic acids are typically located at the terminal end of the glycan structure and because of their acidic, negatively charged nature, sialylated glycoconjugates can inhibit many intermolecular and intercellular reactions. As cited previously, ...
... are necessary for proper mammalian development. Because sialic acids are typically located at the terminal end of the glycan structure and because of their acidic, negatively charged nature, sialylated glycoconjugates can inhibit many intermolecular and intercellular reactions. As cited previously, ...
Measurement of apolipoprotein E and amyloid β clearance rates in
... has been applied to quantify protein synthesis and degradation in yeast [11], mammalian cell lines [12,13], and small animals [14,15]. However, protein turnover studies in animals have been limited due to issues with MS detection sensitivity and accurate label quantification, along with difficulties ...
... has been applied to quantify protein synthesis and degradation in yeast [11], mammalian cell lines [12,13], and small animals [14,15]. However, protein turnover studies in animals have been limited due to issues with MS detection sensitivity and accurate label quantification, along with difficulties ...
MICROBIAL PHYSIOLOGY AND BIOCHEMISTRY
... Just like proteins, enzymes can be obtained from a variety of sources, depending on which they can be sub-classified in terms of nomenclature as well as specificity of their activities. It is important to note that activity of enzymes carrying out the same chemical modifications can vary depending o ...
... Just like proteins, enzymes can be obtained from a variety of sources, depending on which they can be sub-classified in terms of nomenclature as well as specificity of their activities. It is important to note that activity of enzymes carrying out the same chemical modifications can vary depending o ...
Plant Mitochondrial Electron Transfer and Molecular
... mitochondria, the AOA antibody binds to from one to three immunoreactive protein bands in the range of 35 to 37 kD, depending on the plant and the tissue from which the mitochondria have been isolated (Elthon et al., 1989a; Obenland et al., 1990; Kearns et al., 1992). The pattern of protein bands ob ...
... mitochondria, the AOA antibody binds to from one to three immunoreactive protein bands in the range of 35 to 37 kD, depending on the plant and the tissue from which the mitochondria have been isolated (Elthon et al., 1989a; Obenland et al., 1990; Kearns et al., 1992). The pattern of protein bands ob ...
Plant Mitochondrial Electron Transfer and Molecular
... mitochondria, the AOA antibody binds to from one to three immunoreactive protein bands in the range of 35 to 37 kD, depending on the plant and the tissue from which the mitochondria have been isolated (Elthon et al., 1989a; Obenland et al., 1990; Kearns et al., 1992). The pattern of protein bands ob ...
... mitochondria, the AOA antibody binds to from one to three immunoreactive protein bands in the range of 35 to 37 kD, depending on the plant and the tissue from which the mitochondria have been isolated (Elthon et al., 1989a; Obenland et al., 1990; Kearns et al., 1992). The pattern of protein bands ob ...
Unit 1 Life Processes and Biochemistry
... Function: make cell parts and carry out chemical reactions Examples: enzymes, hemoglobin, melanin, keratin, insulin How to identify: each amino acid has a N! ...
... Function: make cell parts and carry out chemical reactions Examples: enzymes, hemoglobin, melanin, keratin, insulin How to identify: each amino acid has a N! ...
Directed mutagenesis of the Trypanosoma cruzi trans
... only in retention of sialidase activity (Figure 4). The level of sialyltransferase activity of the P to Q mutant was approximately 25% that of wild-type TS (Figure 2). We had previously described the inability to express enzyme activity from constructs expressing only the catalytic domains (i.e., wi ...
... only in retention of sialidase activity (Figure 4). The level of sialyltransferase activity of the P to Q mutant was approximately 25% that of wild-type TS (Figure 2). We had previously described the inability to express enzyme activity from constructs expressing only the catalytic domains (i.e., wi ...
Molecular signatures-based prediction of enzyme
... level (see previous definitions) was computed between pairs of reactions annotated for each enzyme. Based on reaction similarity, the sequence list was split into positive set (promiscuous) and negative set (non-promiscuous). Redundancy was removed within each set by using the cd-hit program (Li and ...
... level (see previous definitions) was computed between pairs of reactions annotated for each enzyme. Based on reaction similarity, the sequence list was split into positive set (promiscuous) and negative set (non-promiscuous). Redundancy was removed within each set by using the cd-hit program (Li and ...
PROTEIN PHOSPHORYLATION AND CELLULAR REGULATION, I by
... order to obtain pure protein. More puzzling was the fact that the partially pure enzyme, which we did obtain, was always in th e b form rather than the a form. Insofar as we could determine we had followed the Coris’ procedure exactly except that we had clarified the original muscle extract by centr ...
... order to obtain pure protein. More puzzling was the fact that the partially pure enzyme, which we did obtain, was always in th e b form rather than the a form. Insofar as we could determine we had followed the Coris’ procedure exactly except that we had clarified the original muscle extract by centr ...
Lecture 1 - "Hudel" Luecke
... Here are three models of an alpha-helix. The first shows only the alpha-carbon of each amino acid. The second shows all of the atoms that make up the backbone of the polypeptide. The third shows all of the hydrogen bonds that hold alpha-helices together.The third, and most complete, model is also sh ...
... Here are three models of an alpha-helix. The first shows only the alpha-carbon of each amino acid. The second shows all of the atoms that make up the backbone of the polypeptide. The third shows all of the hydrogen bonds that hold alpha-helices together.The third, and most complete, model is also sh ...
Introduction to Fluorescence Techniques
... Environmental Sensitivity of Fluorescence Fluorophore–Fluorophore Interactions Fluorescence quenching can be defined as a bimolecular process that reduces the fluorescence quantum yield without changing the fluorescence emission spectrum; it can result from transient excited-state interactions (coll ...
... Environmental Sensitivity of Fluorescence Fluorophore–Fluorophore Interactions Fluorescence quenching can be defined as a bimolecular process that reduces the fluorescence quantum yield without changing the fluorescence emission spectrum; it can result from transient excited-state interactions (coll ...
Document
... 60 year old homeless person with open sores. Marseille, France (EID 2009: 15;985) 70 year old homeless male with open sores. Buenos Aries, Argentina (JCM 49; 2011: 2333-2335) 83 year old male “questionably competent” with open sores. – Burlington, WI ...
... 60 year old homeless person with open sores. Marseille, France (EID 2009: 15;985) 70 year old homeless male with open sores. Buenos Aries, Argentina (JCM 49; 2011: 2333-2335) 83 year old male “questionably competent” with open sores. – Burlington, WI ...
Farnesyl diphosphate synthase, the target for nitrogen
... eukaryotic cells to convert acetyl-CoA into FDP (farnesyl diphosphate). FDP is mainly used for biosynthesis of sterols but it may also be used, either directly or after conversion into geranylgeranyl diphosphate, for protein prenylation [1]. The mevalonate pathway also provides precursors for synthe ...
... eukaryotic cells to convert acetyl-CoA into FDP (farnesyl diphosphate). FDP is mainly used for biosynthesis of sterols but it may also be used, either directly or after conversion into geranylgeranyl diphosphate, for protein prenylation [1]. The mevalonate pathway also provides precursors for synthe ...
Enzyme inhibitor
... In drug design it is important to consider the concentrations of substrates to which the target enzymes are exposed. For example, some protein kinase inhibitors have chemical structures that are similar to adenosine triphosphate, one of the substrates of these enzymes. However, drugs that are simple ...
... In drug design it is important to consider the concentrations of substrates to which the target enzymes are exposed. For example, some protein kinase inhibitors have chemical structures that are similar to adenosine triphosphate, one of the substrates of these enzymes. However, drugs that are simple ...
- Sportscience
... balance during intense resistance and/or endurance training, athletes should ingest approximately 1.3 to 1.8 g protein per kg body mass per day (Butterfield, 1991; Lemon, 1998; Kreider et al., 1993; Kreider, 1999). Athletes training at high-altitude may need as much as 2.2 g protein per kg per day i ...
... balance during intense resistance and/or endurance training, athletes should ingest approximately 1.3 to 1.8 g protein per kg body mass per day (Butterfield, 1991; Lemon, 1998; Kreider et al., 1993; Kreider, 1999). Athletes training at high-altitude may need as much as 2.2 g protein per kg per day i ...
The mechanism of Stx2 enrichment in outer membrane vesicles of
... In this aim, we will purify OMVs and periplasm from E. coli O157:H7 and use Western blotting to confirm that Stx1 is present at a higher concentration in the OMVs than in the periplasm. To begin the identification of proteins that interact with Stx1 and are present in the OMVs, we will use velocity ...
... In this aim, we will purify OMVs and periplasm from E. coli O157:H7 and use Western blotting to confirm that Stx1 is present at a higher concentration in the OMVs than in the periplasm. To begin the identification of proteins that interact with Stx1 and are present in the OMVs, we will use velocity ...
Annotation guidelines - Systems Biology and Bioinformatics
... (b) Substitution of pyridoxal 5'-phosphate in D-serine dehydratase from Escherichia coli by cofactor analogues provides information on cofactor binding and catalysis. As shown above in (b), at first we consider D-serine dehydratase from Escherichia coli as a potential GP entity. As stated above, how ...
... (b) Substitution of pyridoxal 5'-phosphate in D-serine dehydratase from Escherichia coli by cofactor analogues provides information on cofactor binding and catalysis. As shown above in (b), at first we consider D-serine dehydratase from Escherichia coli as a potential GP entity. As stated above, how ...
Scientific Opinion on Dietary Reference Values for protein
... but the available data were considered insufficient to establish DRVs. For adults, the Panel accepted the value of 0.66 g protein/kg body weight per day based on a meta-analysis of nitrogen balance data as the average requirement (AR). In healthy adults, the protein requirement per kg body weight is ...
... but the available data were considered insufficient to establish DRVs. For adults, the Panel accepted the value of 0.66 g protein/kg body weight per day based on a meta-analysis of nitrogen balance data as the average requirement (AR). In healthy adults, the protein requirement per kg body weight is ...
Src protein–tyrosine kinase structure and regulation
... SH3 domains (60 amino acid residues) bind to sequences that can adopt a left-handed helical conformation. These sequences are usually rich in proline. RPLPPLP is an optimal Class 1 Src SH3 ligand [2,14]. UPPLPXR is an optimal Class 2 Src SH3 ligand that binds with an inverted orientation on the SH3 ...
... SH3 domains (60 amino acid residues) bind to sequences that can adopt a left-handed helical conformation. These sequences are usually rich in proline. RPLPPLP is an optimal Class 1 Src SH3 ligand [2,14]. UPPLPXR is an optimal Class 2 Src SH3 ligand that binds with an inverted orientation on the SH3 ...
thèse - Université Evry Val d`Essonne
... oxidative phosphorylation (OXPHOS), twenty two tRNAs and three rRNAs, one small ribosomal subunit protein (Var1) is also of mitochondrial origin (Fig. I-2B). The complete sequencing of several mammalian mtDNAs, including human, also revealed a small number of proteins (thirteen in humans) that are s ...
... oxidative phosphorylation (OXPHOS), twenty two tRNAs and three rRNAs, one small ribosomal subunit protein (Var1) is also of mitochondrial origin (Fig. I-2B). The complete sequencing of several mammalian mtDNAs, including human, also revealed a small number of proteins (thirteen in humans) that are s ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.