Biophysical Society Poster, 2004
... used to assess the depth of penetration of protein residues into membranes combined with site direct spin labeling and EPR-based collisional relaxation measurements. These studies have been very useful in characterizing the nature of protein-membrane interactions and in determining the orientation o ...
... used to assess the depth of penetration of protein residues into membranes combined with site direct spin labeling and EPR-based collisional relaxation measurements. These studies have been very useful in characterizing the nature of protein-membrane interactions and in determining the orientation o ...
Amino acid
... that recurs in different contexts in different proteins – A region with a separate hydrophobic core ...
... that recurs in different contexts in different proteins – A region with a separate hydrophobic core ...
Protein - Creating Vitality
... Protein Protein was the first substance to be recognized as a vital part of living tissue. The name was derived from a Greek word meaning “of first importance.” Proteins are the building blocks of many structures in our bodies, and half of our dry body weight (water taken out) is made of protein. Mo ...
... Protein Protein was the first substance to be recognized as a vital part of living tissue. The name was derived from a Greek word meaning “of first importance.” Proteins are the building blocks of many structures in our bodies, and half of our dry body weight (water taken out) is made of protein. Mo ...
CHAPTER 6
... dimeric protein that can exist in either of two conformational states, R or T. Each subunit in the dimer has a binding site for substrate S and an allosteric effector site, F. The promoters are symmetrically related to one another in the protein, and symmetry is conserved regardless of the conformat ...
... dimeric protein that can exist in either of two conformational states, R or T. Each subunit in the dimer has a binding site for substrate S and an allosteric effector site, F. The promoters are symmetrically related to one another in the protein, and symmetry is conserved regardless of the conformat ...
European Journal of Biochemistry
... pore protein)The rate of permeation of j-lactam antibiotics through the outer membrane of intact cells and its inhibition by polyphosphate was measured as described by Overbeeke et al. [16]. ...
... pore protein)The rate of permeation of j-lactam antibiotics through the outer membrane of intact cells and its inhibition by polyphosphate was measured as described by Overbeeke et al. [16]. ...
The Effects of Whey Supplementation and Natural Diet on Protein
... Nutrition plays an important role in the promotion of training adaptations. According to The Institute of Medicine, 10-35% of daily calories for an average adult should come from protein, so active adults should be between 25-35%. The amount of protein that an athlete needs depends on a few factors: ...
... Nutrition plays an important role in the promotion of training adaptations. According to The Institute of Medicine, 10-35% of daily calories for an average adult should come from protein, so active adults should be between 25-35%. The amount of protein that an athlete needs depends on a few factors: ...
Ch 16.4 Enzymes and rest
... Warm-up Sort the following biological molecule into the their respective biochemical substance ...
... Warm-up Sort the following biological molecule into the their respective biochemical substance ...
Plant mitochondria contain the protein translocase subunits TatB
... GFP targeting analysis. Firstly, we cloned the full length coding sequence for At5g43680 in frame with a C-terminally located GFP tag. Tobacco leaves were infiltrated with agrobacterium carrying the At5g43680-GFP construct. Analysis of the GFP expression of protoplasts isolated from the transformed ...
... GFP targeting analysis. Firstly, we cloned the full length coding sequence for At5g43680 in frame with a C-terminally located GFP tag. Tobacco leaves were infiltrated with agrobacterium carrying the At5g43680-GFP construct. Analysis of the GFP expression of protoplasts isolated from the transformed ...
Chapter 17: Gene Expression Gene Expression DNA houses all
... Complimentary regions allow it to H-bond & fold over on itself o Anti-codon – one end has 3 nucleotide segment to match mRNA codon (61) o 3’ end extends off other end & will bind an amino acid o 45-50 human tRNA (vs. 61 AA codons) due to ‘wobble’ o Aminoacyl-tRNA Synthetase Enzyme that catalyzes ...
... Complimentary regions allow it to H-bond & fold over on itself o Anti-codon – one end has 3 nucleotide segment to match mRNA codon (61) o 3’ end extends off other end & will bind an amino acid o 45-50 human tRNA (vs. 61 AA codons) due to ‘wobble’ o Aminoacyl-tRNA Synthetase Enzyme that catalyzes ...
Nucleic Acids Research
... (3,4). Indeed, large patches of positive charges have been suggested to be characteristic of protein–nucleic acid interfaces (5–8). Recently, several methods have been developed for automatic prediction of DNA-binding proteins based on the existence of large positive patches on the protein surface ( ...
... (3,4). Indeed, large patches of positive charges have been suggested to be characteristic of protein–nucleic acid interfaces (5–8). Recently, several methods have been developed for automatic prediction of DNA-binding proteins based on the existence of large positive patches on the protein surface ( ...
Protein Use During Training
... The most dramatic proof of protein's benefits in a sports drink was shown in a study conducted at the University of Texas. Researchers compared the effects of a conventional 7.75% carbohydrate sports drink and a carbohydrate-protein sports drink containing the same amount of calories in cyclists dur ...
... The most dramatic proof of protein's benefits in a sports drink was shown in a study conducted at the University of Texas. Researchers compared the effects of a conventional 7.75% carbohydrate sports drink and a carbohydrate-protein sports drink containing the same amount of calories in cyclists dur ...
Protein synthesis
... During the 1950s and 1960s, it became apparent that DNA is essential in the synthesis of proteins. Proteins are used in enzymes and as structural materials in cells. Many specialized proteins function in cellular activities. For example, in humans, the hormone insulin and the muscle cell filaments a ...
... During the 1950s and 1960s, it became apparent that DNA is essential in the synthesis of proteins. Proteins are used in enzymes and as structural materials in cells. Many specialized proteins function in cellular activities. For example, in humans, the hormone insulin and the muscle cell filaments a ...
Force Fields
... Force fields do not need to be based on concepts of physics. You can also base a FF on statistics. The idea being that if you see it often, it must have a high probability. So, a variant on the sequence rule: If it is important, you see it often. And now we will do an experiment counting sheep. ...
... Force fields do not need to be based on concepts of physics. You can also base a FF on statistics. The idea being that if you see it often, it must have a high probability. So, a variant on the sequence rule: If it is important, you see it often. And now we will do an experiment counting sheep. ...
Extended Detail on Checklist items
... is somewhat arbitrary but well accepted and remains set as the upper limit. For many datasets from modern instrumentation, achieving a 1% global FDR may include very low quality results with a local FDR worse than 10%, which is undesirable. A global FDR lower than 1% is encouraged, but it should nev ...
... is somewhat arbitrary but well accepted and remains set as the upper limit. For many datasets from modern instrumentation, achieving a 1% global FDR may include very low quality results with a local FDR worse than 10%, which is undesirable. A global FDR lower than 1% is encouraged, but it should nev ...
The epidermal intermediate filament proteins of
... the polyclonal antibodies was tested by immunoblotting. ...
... the polyclonal antibodies was tested by immunoblotting. ...
Nutrients - HRSBSTAFF Home Page
... example: shivering in response to cooling of body during cold weather or sweating when their core temperature gets too hot. Homeostasis allows an organism to remain in balance with its environment. If homeostasis is not maintained, it can harm or kill the organism. ...
... example: shivering in response to cooling of body during cold weather or sweating when their core temperature gets too hot. Homeostasis allows an organism to remain in balance with its environment. If homeostasis is not maintained, it can harm or kill the organism. ...
Protein Use during Training
... Furthermore, a key reason why protein may be effective in enhancing endurance is its ability to stimulate insulin, a hormone responsible for transferring carbohydrate from the blood into the muscles, where it can be converted to energy. When carbohydrate is consumed, insulin levels increase, resulti ...
... Furthermore, a key reason why protein may be effective in enhancing endurance is its ability to stimulate insulin, a hormone responsible for transferring carbohydrate from the blood into the muscles, where it can be converted to energy. When carbohydrate is consumed, insulin levels increase, resulti ...
Regulation of the Discs Large Tumor Suppressor by a
... and -catenin (4) by specifically recognizing the serine-phosphorylated motif DSGXXS within these proteins. -Catenin has a dual role: a membrane pool participates in formation of adherens junctions, whereas a short-lived soluble pool functions as signal transducer/transcription factor to promote pr ...
... and -catenin (4) by specifically recognizing the serine-phosphorylated motif DSGXXS within these proteins. -Catenin has a dual role: a membrane pool participates in formation of adherens junctions, whereas a short-lived soluble pool functions as signal transducer/transcription factor to promote pr ...
QIAxcel® system — linkage analysis of zebrafish mutants
... the QIAxcel system, we were able to resolve size differences of DNA fragment s down to a few base pairs, a resolution that was not attainable using conventional agarose gel electrophoresis. The QIAxcel system provides significant advantages in determination of genotype for gene linkage studies. ...
... the QIAxcel system, we were able to resolve size differences of DNA fragment s down to a few base pairs, a resolution that was not attainable using conventional agarose gel electrophoresis. The QIAxcel system provides significant advantages in determination of genotype for gene linkage studies. ...
Identification and Characterization of Two Temperature
... 8.8–6 M urea–30% (vol/vol) glycerol–2% (wt/vol) SDS, placed on SDS–10% PAGE gel, and run for 15 min at 20 mA followed by 4 h at 40 mA. Proteins separated by 1-D and 2-D electrophoresis were visualized by silver staining according to the method of Blum et al. (7). For immunoblotting, electrophoretica ...
... 8.8–6 M urea–30% (vol/vol) glycerol–2% (wt/vol) SDS, placed on SDS–10% PAGE gel, and run for 15 min at 20 mA followed by 4 h at 40 mA. Proteins separated by 1-D and 2-D electrophoresis were visualized by silver staining according to the method of Blum et al. (7). For immunoblotting, electrophoretica ...
F factor conjugation is a true type IV secretion system
... Homology studies have revealed that the pilin gene appears to have been shu¥ed among various T4SS during evolution. For example, the IncHI1 plasmid, R27, has an F-like T4SS except for a P-like pilin protein and corresponding peptidase/cyclase [34]. The lack of sequence conservation in pilin could be ...
... Homology studies have revealed that the pilin gene appears to have been shu¥ed among various T4SS during evolution. For example, the IncHI1 plasmid, R27, has an F-like T4SS except for a P-like pilin protein and corresponding peptidase/cyclase [34]. The lack of sequence conservation in pilin could be ...
FST Human Follistatin Human Recombinant Catalog No. CB
... Follistatin Human Recombinant produced in E. coli is a single, non-glycosylated polypeptide chain containing 288 amino acids and having a total molecular mass of 31.5kDa. The FST is purified by proprietary chromatographic techniques. Source: Escherichia coli Physical Appearance: Sterile Filtered Whi ...
... Follistatin Human Recombinant produced in E. coli is a single, non-glycosylated polypeptide chain containing 288 amino acids and having a total molecular mass of 31.5kDa. The FST is purified by proprietary chromatographic techniques. Source: Escherichia coli Physical Appearance: Sterile Filtered Whi ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.