Caught in the act – modelling how a biological catalyst works
... CSAR T3E has provided a detailed picture of a reaction in an important enzyme, showing at the atomic level how transition state stabilization is achieved 1 . Para-hydroxybenzoate hydroxylase (PHBH) is an important enzyme in the microbial biodegradation of a wide variety of aromatic chemicals, includ ...
... CSAR T3E has provided a detailed picture of a reaction in an important enzyme, showing at the atomic level how transition state stabilization is achieved 1 . Para-hydroxybenzoate hydroxylase (PHBH) is an important enzyme in the microbial biodegradation of a wide variety of aromatic chemicals, includ ...
Notes - Part 1.
... 4.3 Reverse turns When torsion angles differ in three or four consecutive amino acid residues, the polypeptide chains can change direction. Some combinations of torsion angles reoccur throughout globular proteins, and probably in some fibrous proteins like amyloid, giving rise to right angle bends ( ...
... 4.3 Reverse turns When torsion angles differ in three or four consecutive amino acid residues, the polypeptide chains can change direction. Some combinations of torsion angles reoccur throughout globular proteins, and probably in some fibrous proteins like amyloid, giving rise to right angle bends ( ...
1 Supplementary Model Description
... contractility in both smooth muscle and nonmuscle cells [11-13]. MLC phosphorylation is regulated by the balance of two enzymatic activities, i.e., Myosin light chain kinase (MLCK) and myosin phosphatase (MYCP). MLCK is regulated by Ca2+ /calmodulin and is believed to be a major kinase in both smoot ...
... contractility in both smooth muscle and nonmuscle cells [11-13]. MLC phosphorylation is regulated by the balance of two enzymatic activities, i.e., Myosin light chain kinase (MLCK) and myosin phosphatase (MYCP). MLCK is regulated by Ca2+ /calmodulin and is believed to be a major kinase in both smoot ...
Protein Folding Cell and Mol Biology Lab
... in the structure. In the "Find" dialog box, ask it to look for the two strings "pro" and "202" ("find" is not sensitive to upper vs lower case). It will put a marker on the point, or center it if you turn on pickcenter. "Find" acts just like a mouse-click, so you can also use it to do things like ad ...
... in the structure. In the "Find" dialog box, ask it to look for the two strings "pro" and "202" ("find" is not sensitive to upper vs lower case). It will put a marker on the point, or center it if you turn on pickcenter. "Find" acts just like a mouse-click, so you can also use it to do things like ad ...
Activity: Poisonous mushrooms worksheet
... 4 There is a delay of 2–6 days between eating fungi containing -amanitin and the development of symptoms associated with liver damage. (a) Explain why this delay might suggest that it would be inappropriate to treat patients with substances that remove poisons from the gut. (2 marks) (b) In spite o ...
... 4 There is a delay of 2–6 days between eating fungi containing -amanitin and the development of symptoms associated with liver damage. (a) Explain why this delay might suggest that it would be inappropriate to treat patients with substances that remove poisons from the gut. (2 marks) (b) In spite o ...
Nutrition for Life: The food we eat
... They can cause heart disease if intake is not monitored. Since 2006, all food labels MUST include quantity of trans fats. (Look for hydrogenated or partially hydrogenated) ...
... They can cause heart disease if intake is not monitored. Since 2006, all food labels MUST include quantity of trans fats. (Look for hydrogenated or partially hydrogenated) ...
Dr. John Perozich's Full CV
... Protein Bioinformatics. Supervised undergraduate research projects. Each student ...
... Protein Bioinformatics. Supervised undergraduate research projects. Each student ...
Molecules of Life
... array held in place by hydrogen bonds (dotted lines) between different parts of the polypeptide chain. ...
... array held in place by hydrogen bonds (dotted lines) between different parts of the polypeptide chain. ...
Document
... array held in place by hydrogen bonds (dotted lines) between different parts of the polypeptide chain. ...
... array held in place by hydrogen bonds (dotted lines) between different parts of the polypeptide chain. ...
Yr12Ch12 - ChemistryVCE
... Proteins are often described in terms of their primary, secondary and tertiary structures. What is the difference between these levels of structure and how does each contribute to the role of the protein? A38. Primary structure: the sequence of the amino acids that make up the polymer chain. This de ...
... Proteins are often described in terms of their primary, secondary and tertiary structures. What is the difference between these levels of structure and how does each contribute to the role of the protein? A38. Primary structure: the sequence of the amino acids that make up the polymer chain. This de ...
Macromolecules PPT.
... e.g. Anything with a functional group HYDROPHOBIC – - not attracted to water because it is non-polar e.g. lipids (oil, fat) ...
... e.g. Anything with a functional group HYDROPHOBIC – - not attracted to water because it is non-polar e.g. lipids (oil, fat) ...
Chapter 12 Pathways to biomolecules
... Proteins are often described in terms of their primary, secondary and tertiary structures. What is the difference between these levels of structure and how does each contribute to the role of the protein? A38. Primary structure: the sequence of the amino acids that make up the polymer chain. This de ...
... Proteins are often described in terms of their primary, secondary and tertiary structures. What is the difference between these levels of structure and how does each contribute to the role of the protein? A38. Primary structure: the sequence of the amino acids that make up the polymer chain. This de ...
Biochemistry Practice Questions
... The enzyme's activity is greater around pH 8.0 than around pH 5.0. ...
... The enzyme's activity is greater around pH 8.0 than around pH 5.0. ...
chapter 5 the structure and function of macromolecules
... In almost every case, the function of a protein depends on its ability to recognize and bind to some other molecule. ○ For example, an antibody binds to a particular foreign substance. ○ Natural signal molecules called endorphins bind to specific receptor proteins on the surface of brain cells in hu ...
... In almost every case, the function of a protein depends on its ability to recognize and bind to some other molecule. ○ For example, an antibody binds to a particular foreign substance. ○ Natural signal molecules called endorphins bind to specific receptor proteins on the surface of brain cells in hu ...
Protein and vegetarian diets - Medical Journal of Australia
... (expressed as a percentage of total energy intake) that would allow for an adequate intake of all the other nutrients. The NRV document notes that while, on average, only 10% of energy need be consumed as protein to meet the physiological need for protein, this level is insufficient to allow for est ...
... (expressed as a percentage of total energy intake) that would allow for an adequate intake of all the other nutrients. The NRV document notes that while, on average, only 10% of energy need be consumed as protein to meet the physiological need for protein, this level is insufficient to allow for est ...
Binding of a Growth Hormone- Inducible Nuclear Factor Is Mediated
... in GH-treated liver nuclear extracts (12). Ultimately, many aspectsof MAP kinase-activatedsignaltransduction are mediatedby Jun/Fos binding to Hela cell activator protein-l (AP-1) elements. As Fos and Jun both demonstrate GH responsiveness(25, 26) this may be an alternative pathway for GH-mediatedge ...
... in GH-treated liver nuclear extracts (12). Ultimately, many aspectsof MAP kinase-activatedsignaltransduction are mediatedby Jun/Fos binding to Hela cell activator protein-l (AP-1) elements. As Fos and Jun both demonstrate GH responsiveness(25, 26) this may be an alternative pathway for GH-mediatedge ...
A Conserved Family of Nuclear Proteins Containing
... by several maternally active genes (see above), by Kr, a blastoderm gastrulation-specific segmentation gene, and by the Kr h gene, which extends its action into later embryonic stages, encouraged studies with the anti-H/C link antibodies on polytene salivary gland chromosomes. If the antigen were pr ...
... by several maternally active genes (see above), by Kr, a blastoderm gastrulation-specific segmentation gene, and by the Kr h gene, which extends its action into later embryonic stages, encouraged studies with the anti-H/C link antibodies on polytene salivary gland chromosomes. If the antigen were pr ...
Biogenesis of MalF and the MalFGK Maltose Transport Escherichia coli 2
... from Abcam. Antisera against SecY, SecE, YidC, and Ffh were from our own collection. Antisera against MalF, -G, and -K, trigger factor (TF), and L23 were kind gifts from A. Davidson, W. Wickner, and R. Brimacombe, respectively. In Vitro Cross-linking—E. coli strain MRE600 was used to prepare a lysat ...
... from Abcam. Antisera against SecY, SecE, YidC, and Ffh were from our own collection. Antisera against MalF, -G, and -K, trigger factor (TF), and L23 were kind gifts from A. Davidson, W. Wickner, and R. Brimacombe, respectively. In Vitro Cross-linking—E. coli strain MRE600 was used to prepare a lysat ...
Anti-Vinculin antibody
... Exists in at least two conformations. When in the closed, 'inactive' conformation, extensive interactions between the head and tail domains prevent detectable binding to most of its ligands. It takes on an 'active' conformation after cooperative and simultaneous binding of two different ligands. Thi ...
... Exists in at least two conformations. When in the closed, 'inactive' conformation, extensive interactions between the head and tail domains prevent detectable binding to most of its ligands. It takes on an 'active' conformation after cooperative and simultaneous binding of two different ligands. Thi ...
Evolutionary predictions of binding surfaces and interactions Olivier
... The input to ET consists of a protein family with divergently related sequences in a multiple sequence alignment (MSA) and a related sequence identity tree, which can be generated using programs such as Accelrys’s PILEUP (found in the Genetics Computer Group [GCG®] Wisconsin Package), CLUSTALW and ...
... The input to ET consists of a protein family with divergently related sequences in a multiple sequence alignment (MSA) and a related sequence identity tree, which can be generated using programs such as Accelrys’s PILEUP (found in the Genetics Computer Group [GCG®] Wisconsin Package), CLUSTALW and ...
Understanding the regulation of surfactant gene expression EDITORIAL W. Jacot, J. Bousquet
... alveolar type-II epithelial cells are defined by the synthesis of surfactant proteins. These molecules are implicated in numerous human diseases such as neonatal respiratory distress syndrome [1] and possibly acute respiratory distress syndrome (ARDS) [2], where a better understanding could lead to ...
... alveolar type-II epithelial cells are defined by the synthesis of surfactant proteins. These molecules are implicated in numerous human diseases such as neonatal respiratory distress syndrome [1] and possibly acute respiratory distress syndrome (ARDS) [2], where a better understanding could lead to ...
File
... affinity capture. Mice were immunized with Peb1a, and the sera collected were tested for reactivity against different C. jejuni strains. Mouse serum analyses revealed positive reactivity for all strains, with each individual strain presenting different quantities of expressed Peb1a. Cell fusion tech ...
... affinity capture. Mice were immunized with Peb1a, and the sera collected were tested for reactivity against different C. jejuni strains. Mouse serum analyses revealed positive reactivity for all strains, with each individual strain presenting different quantities of expressed Peb1a. Cell fusion tech ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.