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FST Human Follistatin Human Recombinant Catalog No. CB
FST Human Follistatin Human Recombinant Catalog No. CB

... Follistatin Human Recombinant produced in E. coli is a single, non-glycosylated polypeptide chain containing 288 amino acids and having a total molecular mass of 31.5kDa. The FST is purified by proprietary chromatographic techniques. Source: Escherichia coli Physical Appearance: Sterile Filtered Whi ...
Chapter 23 - UGA Extension
Chapter 23 - UGA Extension

... RESTRICT THE USE OF ANIMAL PRODUCTS TO 3 % OF THE FEED ...
Macromolecules
Macromolecules

... steel rod of the same diameter, yet it is much more elastic, so scientists hope to use it for products as diverse as bulletproof vests and artificial joints. ...
Pierce BCA Protein Assay Kit
Pierce BCA Protein Assay Kit

... The Thermo Scientific™ Pierce™ BCA Protein Assay is a detergent-compatible formulation based on bicinchoninic acid (BCA) for the colorimetric detection and quantitation of total protein. This method combines the well-known reduction of Cu+2 to Cu+1 by protein in an alkaline medium (the biuret reacti ...
AMINO ACIDS COMPLEX Factsheet
AMINO ACIDS COMPLEX Factsheet

... acids linked by peptide bonds. Proteins are not obtained directly from human diet, instead they are broken down from dietary protein into the constituent amino acid, which the body uses to build the specific protein that it needs. Each person’s ability to break down protein into single amino acids d ...
Unit 2 - Protein Synthesis AAB - bushelman-hap
Unit 2 - Protein Synthesis AAB - bushelman-hap

... 1. A second tRNA bonds with the next three bases of the mRNA, the amino acid links onto the amino acid of the first tRNA via a peptide bond. (Reminder) Each tRNA specific for one amino acid only, but some amino acids coded for by up to 6 codons. Order of bases in mRNA codons determine which tRNA ant ...
Translation | Principles of Biology from Nature Education
Translation | Principles of Biology from Nature Education

... Post-translational modifications prepare proteins for their functions. As translation occurs, the polypeptide folds spontaneously due to its primary sequence. Frequently, a chaperone protein called chaperonin also aids in proper folding. However, many proteins require additional modifications after ...
Structural and functional analyses of a yeast mitochondrial
Structural and functional analyses of a yeast mitochondrial

... proteins separated by a Vydac C-18 column with a gradient composed of solution A (0.1 % aqueous TFA, 10% acetonitrile) and solution B (0.1 % TFA, 55% acetonitrile) is shown in Fig. 1. Peaks were pooled, lyophilized, and the degree of purity of proteins in individual peaks was examined by SDS polyacr ...
ab115058 – Histone H3 (pan-methyl K4) Quantification Kit (Colorimetric)
ab115058 – Histone H3 (pan-methyl K4) Quantification Kit (Colorimetric)

Lehninger Principles of Biochemistry
Lehninger Principles of Biochemistry

Machine Learning Designs for Artificial Histone Acetyltransferases
Machine Learning Designs for Artificial Histone Acetyltransferases

... The two different seed training file gives different accuracy value to start of with. In figure 4, the classifier improves its accuracy quickly. It was also shown that using active learning methods is actually better than random selection of data. For the second initial training file, the difference ...
Chemical constituents
Chemical constituents

... [Note] in quantitative test, complete reaction between the reducing sugar and Benedict’s solution is needed, to do this, excess volume of Benedict’s solution should be added; the amount of red precipitate formed is directly proportional to the amount of reducing sugar present in the solution. (ii) C ...
Hydrogen Bonds, Hydrophobicity Forces and the Character of the
Hydrogen Bonds, Hydrophobicity Forces and the Character of the

... – It undergoes a first-order-like folding transition, from an expanded state to the native three-helix-bundle state. – It forms more stable secondary structure than the one- and two-helix segments. Qualitatively similar results have been obtained previously for Cα [6, 17–20] and all-atom [21] off-la ...
Macromolecules - Issaquah Connect
Macromolecules - Issaquah Connect

... saturated fatty acid? (unsaturated has double bonds) Give an example of a food that contains each. 15. Explain why some fatty acids are solid at room temperature while others are liquid. (solid - sat’d - straight) 16. Which type of organisms tend to make saturated fatty acids? (animals) 17. When you ...
Literature 07/19/2010
Literature 07/19/2010

... density on a surface of a glycan array. This feature of presentation was combined with variations in  glycan  structure  and  glycan  density  to  produce  an  array  with  approximately  600  combinations  of  glycan  structure  and  presentation.  The  unique  array  platform  allows  one  to  dis ...
CS689-homology-modeling
CS689-homology-modeling

... improves ability to recognize correct fold versus decoys instead, assume: ...
Synthetic Peptides as Antigens for Antibody Production
Synthetic Peptides as Antigens for Antibody Production

... however, the substantial anticarrier response may mask the frequently weaker anti-peptide response, resulting in few peptide-specific hybridomas being isolated. A variety of alternative approaches to the use of conventional peptide-carrier conjugates have been developed including, for example, the m ...
In vivo characterization of the properties of SUMO1
In vivo characterization of the properties of SUMO1

... the recent development of small proteins, termed monobodies, which are isoform-specific for human SUMO1 and bind with high affinity to the SIM-binding groove [19]. Monobodies are small 94-amino-acid monomeric proteins that do not form any disulfide bonds, so they can be easily purified from Escheric ...
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... theoretical predictions are that we will wind up with a very strong, tough material, with an ability to absorb shock, which is stronger and tougher than the man-made materials that are conventionally available to us,' he says. I The spider is not the only creature that has aroused the interest of ma ...
Amino Acid Analysis
Amino Acid Analysis

Biochem (Test 1)
Biochem (Test 1)

... the claim that different species of organisms use different metabolic strategies to meet their energy requirements for growth, reproduction, and homeostasis? a. During cold periods pond-dwelling animals can increase the number of unsaturated fatty acids in their cell membranes while some plants make ...
7.013 Problem Set 1 - MIT OpenCourseWare
7.013 Problem Set 1 - MIT OpenCourseWare

... What is the effect of Compound Z on Enzyme E4? Of what is this an example? Briefly explain why the cell may need such a regulatory mechanism. ...
Leu-Gly-Phe-Ala-Pro-Gln-Ala. These findings pro
Leu-Gly-Phe-Ala-Pro-Gln-Ala. These findings pro

Whole grains - davis.k12.ut.us
Whole grains - davis.k12.ut.us

... Triglycerides Triglyceride is a form of fat made in the body. Elevated triglycerides can be due to overweight/obesity, physical inactivity, cigarette smoking, excess alcohol consumption and a diet very high in carbohydrates. People with high triglycerides often have a high total cholesterol level. M ...
Yougang Mao Page 1 Yougang Mao 502 Cato St., Pittsburgh, PA
Yougang Mao Page 1 Yougang Mao 502 Cato St., Pittsburgh, PA

... Crucial contributions include two new formulas which can be used to study inter- and intramolecular interaction by NMR spin lattice relaxation time and REDOR experiments., the icesurface adsorption enhanced colligative model that can be used to detect the protein concentration on ice surface, a new ...
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Western blot



The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.
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