... 10. The high rate of the formation of HIV viruses that are resistant to drugs is due to: a) Induction of mutations in the viral genome (DNA) by the drugs. b) Interference of drugs with proofreading ability of PolI. c) Interference of drugs with proofreading ability of HIV reverse transcriptase. d) L ...
locating domains
... • If the sequence has more than about 500 amino acids, it is almost certain that it will be divided into discrete functional domains. If possible, it is preferable to split such large proteins up and consider each domain separately. One can predict the location of domains in a few different ways. Th ...
... • If the sequence has more than about 500 amino acids, it is almost certain that it will be divided into discrete functional domains. If possible, it is preferable to split such large proteins up and consider each domain separately. One can predict the location of domains in a few different ways. Th ...
Human CHMP6, a myristoylated ESCRT-III protein, interacts directly
... glycine), was obtained by PCR-based site-directed mutagenesis using a QuikChange site-directed mutagenesis kit (Stratagene) according to the manufacturer’s instructions, and the mutation was confirmed by DNA sequencing. Bacterial expression plasmids for preparation of recombinant CHMP6 proteins fuse ...
... glycine), was obtained by PCR-based site-directed mutagenesis using a QuikChange site-directed mutagenesis kit (Stratagene) according to the manufacturer’s instructions, and the mutation was confirmed by DNA sequencing. Bacterial expression plasmids for preparation of recombinant CHMP6 proteins fuse ...
to the complete text
... direct binding of these components has been demonstrated. Thus, sorting of membrane cargo could occur by a direct interaction between the cytoplasmic domain of the cargo protein and coat components. Sorting of soluble cargo requires involvement of transmembrane receptors, which might couple sorting ...
... direct binding of these components has been demonstrated. Thus, sorting of membrane cargo could occur by a direct interaction between the cytoplasmic domain of the cargo protein and coat components. Sorting of soluble cargo requires involvement of transmembrane receptors, which might couple sorting ...
How To Explore a Topic of Interest at a Molecular Level Learning
... UniProt (http://www.uniprot.org/) - includes sequence, information of proteins along with functions, disease associations and other information. Can download sequence information from here RCSB PDB (www.rcsb.org) - includes access to 3D structures of bio-macromolecules (e.g. proteins and Nucleic Aci ...
... UniProt (http://www.uniprot.org/) - includes sequence, information of proteins along with functions, disease associations and other information. Can download sequence information from here RCSB PDB (www.rcsb.org) - includes access to 3D structures of bio-macromolecules (e.g. proteins and Nucleic Aci ...
Structures and mechanisms
... activities of enzymes are determined by their three-dimensional structure.[20] However, although structure does determine function, predicting a novel enzyme's activity just from its structure is a very difficult problem that has not yet been solved.[21] Most enzymes are much larger than the substra ...
... activities of enzymes are determined by their three-dimensional structure.[20] However, although structure does determine function, predicting a novel enzyme's activity just from its structure is a very difficult problem that has not yet been solved.[21] Most enzymes are much larger than the substra ...
Bios 302 FINAL FOR 1999.
... You suggest that she analyze the enzyme for the presence of cobalt. Why do you make this suggestion? f. What one slight modification would you make to one enzyme already present in the glycolytic path so that glucose and mannose could be used equally well as starting points for glycolysis? g. The ge ...
... You suggest that she analyze the enzyme for the presence of cobalt. Why do you make this suggestion? f. What one slight modification would you make to one enzyme already present in the glycolytic path so that glucose and mannose could be used equally well as starting points for glycolysis? g. The ge ...
Lab 7 - Creighton Biology
... Please leave the bottles of NADH, pyruvate and oxaloacetate on ice when not in use. This will help prevent the breakdown of reagents before measurements are made. Follow the specific steps for each assay carefully. Be careful with the pipettes to avoid cross-contamination of samples - use each tip o ...
... Please leave the bottles of NADH, pyruvate and oxaloacetate on ice when not in use. This will help prevent the breakdown of reagents before measurements are made. Follow the specific steps for each assay carefully. Be careful with the pipettes to avoid cross-contamination of samples - use each tip o ...
Sequence to Structure Analysis of DOPA Protein from Mucuna
... L-DOPA, (L-3, 4-dihydroxyphenylalanine), an anti-nutritional compound is an important intermediate of secondary metabolism in higher plants and is known as a precursor of alkaloids, betalain, melanine, and others. We analyzed the amino acid sequence of DOPA protein from M. pruriens by using computat ...
... L-DOPA, (L-3, 4-dihydroxyphenylalanine), an anti-nutritional compound is an important intermediate of secondary metabolism in higher plants and is known as a precursor of alkaloids, betalain, melanine, and others. We analyzed the amino acid sequence of DOPA protein from M. pruriens by using computat ...
Cell Membrane Structure and Function
... can also move across membranes by facilitated diffusion, but only down their concentration gradients. The proteins involved undergo configurational changes during the process (recall revolving door analogy). In some cases the proteins facilitating particle movement are specific, i.e., allowing only ...
... can also move across membranes by facilitated diffusion, but only down their concentration gradients. The proteins involved undergo configurational changes during the process (recall revolving door analogy). In some cases the proteins facilitating particle movement are specific, i.e., allowing only ...
Plant mitochondria contain the protein translocase subunits TatB
... GFP targeting analysis. Firstly, we cloned the full length coding sequence for At5g43680 in frame with a C-terminally located GFP tag. Tobacco leaves were infiltrated with agrobacterium carrying the At5g43680-GFP construct. Analysis of the GFP expression of protoplasts isolated from the transformed ...
... GFP targeting analysis. Firstly, we cloned the full length coding sequence for At5g43680 in frame with a C-terminally located GFP tag. Tobacco leaves were infiltrated with agrobacterium carrying the At5g43680-GFP construct. Analysis of the GFP expression of protoplasts isolated from the transformed ...
Disruption of CEP290 microtubule/membrane-binding domains
... CEP290 as an integral structural and regulatory component of the cilium and provide insight into the pathological mechanisms of LCA and related ciliopathies. Further, these data illustrate that disruption of particular CEP290 functional domains may lead to particular disease phenotypes and suggest i ...
... CEP290 as an integral structural and regulatory component of the cilium and provide insight into the pathological mechanisms of LCA and related ciliopathies. Further, these data illustrate that disruption of particular CEP290 functional domains may lead to particular disease phenotypes and suggest i ...
Gene Section UBE3A (ubiquitin protein ligase E3A) Atlas of Genetics and Cytogenetics
... The E6 oncoprotein of so-called high risk HPVs that have been etiologically associated with malignant lesion of the anogenital tract (most notably, cervical cancer) have the ability to bind to E6AP. The E6/E6AP complex binds to the p53 tumor suppressor, thereby targeting p53 for ubiquitylation and p ...
... The E6 oncoprotein of so-called high risk HPVs that have been etiologically associated with malignant lesion of the anogenital tract (most notably, cervical cancer) have the ability to bind to E6AP. The E6/E6AP complex binds to the p53 tumor suppressor, thereby targeting p53 for ubiquitylation and p ...
Proteomic Mapping of Mitochondria in Living Cells
... To examine whether APEX could be employed for proteomic labeling (Fig. 1A), we targeted APEX to the mitochondrial matrix of human embryonic kidney (HEK) cells and initiated labeling by adding biotin-phenol and 1 mM H2O2 to the cell medium. Labeling was terminated after 1 min by cell fixation or lys ...
... To examine whether APEX could be employed for proteomic labeling (Fig. 1A), we targeted APEX to the mitochondrial matrix of human embryonic kidney (HEK) cells and initiated labeling by adding biotin-phenol and 1 mM H2O2 to the cell medium. Labeling was terminated after 1 min by cell fixation or lys ...
Methods and approaches for the comprehensive characterization
... then compared with publicly available protein sequence information. The observed masses of the proteolytic fragments are compared with theoretical in silico sequences to identify the peptide sequence and thereby the protein (14, 29, 36, 44, 65, 77). With the advancements in the separation techniques ...
... then compared with publicly available protein sequence information. The observed masses of the proteolytic fragments are compared with theoretical in silico sequences to identify the peptide sequence and thereby the protein (14, 29, 36, 44, 65, 77). With the advancements in the separation techniques ...
Curriculum for UG
... b. Demonstrate the skills of solving clinical problems and decision making. (C) Integration The knowledge acquired in biochemistry should help the students to integrate molecular events with structure and function of the human body in health and disease. Specific learning objectives ...
... b. Demonstrate the skills of solving clinical problems and decision making. (C) Integration The knowledge acquired in biochemistry should help the students to integrate molecular events with structure and function of the human body in health and disease. Specific learning objectives ...
The Chemical Building Blocks chapt03
... Polymerization of Nucleotides to Make Nucleic Acids (DNA and RNA) • Nucleotides are covalently bound together into long strands through a Dehydration Reaction • The Phosphate of one Nucleotide is bound to the Ribose Sugar of an adjacent nucleotide • These Phosphate-Ribose bonds form the backbones o ...
... Polymerization of Nucleotides to Make Nucleic Acids (DNA and RNA) • Nucleotides are covalently bound together into long strands through a Dehydration Reaction • The Phosphate of one Nucleotide is bound to the Ribose Sugar of an adjacent nucleotide • These Phosphate-Ribose bonds form the backbones o ...
Chapter 3 - Cell Protein Production
... amino acid it detaches from the ribosome and can pick up another amino acid from the cytoplasm • One mRNA often has 10 or 20 ribosomes reading its code at the same time • This means that a cell could produce over 150 000 protein molecules per second ...
... amino acid it detaches from the ribosome and can pick up another amino acid from the cytoplasm • One mRNA often has 10 or 20 ribosomes reading its code at the same time • This means that a cell could produce over 150 000 protein molecules per second ...
Chapter 5 – The Structure and Function of Macromolecules
... Protein functions include structural support, storage, transport, cellular signaling, movement, and defense against foreign ...
... Protein functions include structural support, storage, transport, cellular signaling, movement, and defense against foreign ...
Ch. 2 Macromolecules
... Carbohydrates ! Contain carbon, hydrogen, and oxygen ! Include sugars and starches ! Classified according to size ...
... Carbohydrates ! Contain carbon, hydrogen, and oxygen ! Include sugars and starches ! Classified according to size ...
n-formyl methionine
... Formylmethionine (fMet) is an amino acid found in all living cells. It is a derivative of the amino acid methionine. It is a modified form of methionine in which a formyl group has been added to methionine's amino group. It plays a crucial part in the protein synthesis of bacteria, mitochondria and ...
... Formylmethionine (fMet) is an amino acid found in all living cells. It is a derivative of the amino acid methionine. It is a modified form of methionine in which a formyl group has been added to methionine's amino group. It plays a crucial part in the protein synthesis of bacteria, mitochondria and ...
cAMP-Dependent Protein Kinase, Catalytic Subunit Product
... Activity Assay: cAMP-Dependent Protein Kinase activity is determined in a 60µl reaction containing 40mM Tris-HCl (pH 7.4), 20mM magnesium acetate, 0.2mM [g-32P]ATP (500–1,000cpm/pmol) and 130µM Kemptide. The reaction is incubated for 5 minutes at 30°C and is terminated by spotting 40µl of the reacti ...
... Activity Assay: cAMP-Dependent Protein Kinase activity is determined in a 60µl reaction containing 40mM Tris-HCl (pH 7.4), 20mM magnesium acetate, 0.2mM [g-32P]ATP (500–1,000cpm/pmol) and 130µM Kemptide. The reaction is incubated for 5 minutes at 30°C and is terminated by spotting 40µl of the reacti ...
TNT SP6 High-Yield Wheat Germ Protein Expression System
... 1. Plasmid DNA can be purified using the PureYield™ Plasmid Midiprep and Maxiprep Systems (Cat.# A2492 and A2392, respectively), as well as other standard methods. Plasmid DNA added to the TNT® SP6 High-Yield Wheat Germ Master Mix should be of high quality with minimal salt and RNase carryover. To t ...
... 1. Plasmid DNA can be purified using the PureYield™ Plasmid Midiprep and Maxiprep Systems (Cat.# A2492 and A2392, respectively), as well as other standard methods. Plasmid DNA added to the TNT® SP6 High-Yield Wheat Germ Master Mix should be of high quality with minimal salt and RNase carryover. To t ...
MCB_5255_files/feb 4 2015 MCB 5255 presentation
... Hsp90α, MMP-2 and several co-chaperones are present in the conditioned media of breast cancer cells. Lysate and conditioned media were collected from MDA-MB-231 cells. 20 µg of protein was loaded into each lysate lane and 80 µg of protein was loaded into each conditioned media lane and immunoblotte ...
... Hsp90α, MMP-2 and several co-chaperones are present in the conditioned media of breast cancer cells. Lysate and conditioned media were collected from MDA-MB-231 cells. 20 µg of protein was loaded into each lysate lane and 80 µg of protein was loaded into each conditioned media lane and immunoblotte ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.