Actin
... Dry-cured ham is a traditional food requiring a long processing period for development of its appreciated texture and flavour characteristics. During this time, there is an intense degradation of muscle proteins due to the action of endogenous proteolytic enzymes. This gives rise to an important gen ...
... Dry-cured ham is a traditional food requiring a long processing period for development of its appreciated texture and flavour characteristics. During this time, there is an intense degradation of muscle proteins due to the action of endogenous proteolytic enzymes. This gives rise to an important gen ...
application of recombinant smr-domain containing protein of
... followed by sonication as described above. After centrifugation at 10,000g for 30 minutes at 4ºC, soluble recombinant His6 fusion protein was purified using NiNTA chromatography according to the manufacturer ’s instructions (Ni-NTA ProBond, Invitrogen, CA). SDS-PAGE and immuno-characterization of re ...
... followed by sonication as described above. After centrifugation at 10,000g for 30 minutes at 4ºC, soluble recombinant His6 fusion protein was purified using NiNTA chromatography according to the manufacturer ’s instructions (Ni-NTA ProBond, Invitrogen, CA). SDS-PAGE and immuno-characterization of re ...
The complete nucleotide sequence of cucumber green mottle
... purified virus particles and subjected to agarose gel electrophoresis. Two main R N A bands were detected (data not shown). The main larger one corresponded to full-length genomic RNA. The smaller R N A band was presumed to be derived from short virus particles as shown by Okada et al. (1980) and Fu ...
... purified virus particles and subjected to agarose gel electrophoresis. Two main R N A bands were detected (data not shown). The main larger one corresponded to full-length genomic RNA. The smaller R N A band was presumed to be derived from short virus particles as shown by Okada et al. (1980) and Fu ...
Lecture 4
... 2. General regulation of plasma glucose Pancreas -----> alpha and beta cells Fig. 9-1 and 9-2 (Metab.) 3. Normal and abnormal plasma glucose levels -----> endocrine dysfunction/cell response dysfunction Fig 9-4 (Metab.) 4. Metabolic consequences of insulin insufficiency Fig. 9-3 (Metab) Types of Dia ...
... 2. General regulation of plasma glucose Pancreas -----> alpha and beta cells Fig. 9-1 and 9-2 (Metab.) 3. Normal and abnormal plasma glucose levels -----> endocrine dysfunction/cell response dysfunction Fig 9-4 (Metab.) 4. Metabolic consequences of insulin insufficiency Fig. 9-3 (Metab) Types of Dia ...
Preface 1 PDF
... Over the years, matrix metalloproteinases (MMPs) have gained considerable attention due to their suspected causative role in many destructive conditions, and this association has led to the oft-held idea that MMPs are the principal mediate of matrix turnover and degradation. However, in Chap. 1, Sea ...
... Over the years, matrix metalloproteinases (MMPs) have gained considerable attention due to their suspected causative role in many destructive conditions, and this association has led to the oft-held idea that MMPs are the principal mediate of matrix turnover and degradation. However, in Chap. 1, Sea ...
Cell Membrane and Sub Cellular Components
... 4. Exocytosis: Just as material can be brought into the cell by invagination and formation of a vesicle, the membrane of a vesicle can be fused with the plasma membrane, extruding its contents to the surrounding medium. This is the process of exocytosis. Exocytosis occurs in various cells to remove ...
... 4. Exocytosis: Just as material can be brought into the cell by invagination and formation of a vesicle, the membrane of a vesicle can be fused with the plasma membrane, extruding its contents to the surrounding medium. This is the process of exocytosis. Exocytosis occurs in various cells to remove ...
Effect of RNAi down-regulation of three lysine-deficient
... This thesis comprises of five chapters of a PhD study that aimed at improving the nutritional value of sorghum grain for food and feed consumption. Chapter 1 is an introduction to the morphology and physiology of the sorghum plant, its commercial and domestic usage. This chapter also reviews the nut ...
... This thesis comprises of five chapters of a PhD study that aimed at improving the nutritional value of sorghum grain for food and feed consumption. Chapter 1 is an introduction to the morphology and physiology of the sorghum plant, its commercial and domestic usage. This chapter also reviews the nut ...
Enzymes I - eCurriculum
... Do not follow Michaelis-Menten kinetics (hyperbolic), they show sigmoideal plots Have two or more subunits The substrate binding sites exhibit co-operativity Are modified by reversible non-covalent binding of regulators Allosteric activators lock the enzyme in a conformation that has high affinity f ...
... Do not follow Michaelis-Menten kinetics (hyperbolic), they show sigmoideal plots Have two or more subunits The substrate binding sites exhibit co-operativity Are modified by reversible non-covalent binding of regulators Allosteric activators lock the enzyme in a conformation that has high affinity f ...
Studying photosynthetic organisms from different angles
... advantages over other plant transgene expression methods: - high level of transgene expression (up to 46 % of tsp) - strong natural gene containment - some posttranscriptional modifications (disulfide bonds, bonds lipidation) - no gene silencing or position effects - allows expression of entire prok ...
... advantages over other plant transgene expression methods: - high level of transgene expression (up to 46 % of tsp) - strong natural gene containment - some posttranscriptional modifications (disulfide bonds, bonds lipidation) - no gene silencing or position effects - allows expression of entire prok ...
Biochemistry Spring 2015 Exam III Name: Points
... Choice A: You run SDS-PAGE gels without and with BME (βmercaptoethanol). Images of the two gels are shown on the right. The left lane contains the standards and the right lane is the sample. The molecular weights of the proteins are indicated on the gel images. What is the minimum quaternary structu ...
... Choice A: You run SDS-PAGE gels without and with BME (βmercaptoethanol). Images of the two gels are shown on the right. The left lane contains the standards and the right lane is the sample. The molecular weights of the proteins are indicated on the gel images. What is the minimum quaternary structu ...
Document
... Enzyme action is determined by enzyme shape; enzyme shape is controlled by its primary structure (its amino acid sequence), all part of the central dogma of modern biology: ...
... Enzyme action is determined by enzyme shape; enzyme shape is controlled by its primary structure (its amino acid sequence), all part of the central dogma of modern biology: ...
Gene Section ACVR2A (activin A receptor, type IIA) in Oncology and Haematology
... Upon binding activin, ACVR2 associates with and phosphorylates ACVR1. ACVR1, in turn, phosphorylates Smad2 and/or Smad3. Phosphorylated Smad2 and Smad3 associate with Smad4, translocate to the nucleus, and regulate gene expression. There may be other non-Smad pathways in activin signal transduction. ...
... Upon binding activin, ACVR2 associates with and phosphorylates ACVR1. ACVR1, in turn, phosphorylates Smad2 and/or Smad3. Phosphorylated Smad2 and Smad3 associate with Smad4, translocate to the nucleus, and regulate gene expression. There may be other non-Smad pathways in activin signal transduction. ...
Peptide Formulation: Challenges and Strategies
... These observations illustrate an important difference in formulating peptides and proteins. With proteins, maintaining the native structure is essential. Analytical methods, usually spectroscopic techniques, need to be available to monitor the retention of native structure. With peptides, the soluti ...
... These observations illustrate an important difference in formulating peptides and proteins. With proteins, maintaining the native structure is essential. Analytical methods, usually spectroscopic techniques, need to be available to monitor the retention of native structure. With peptides, the soluti ...
Protein synthesis (Primer)
... Recurring patterns of main chain conformations stabilized by hydrogen bonds There are two types of secondary structure, i.e. alpha-helix and beta-sheet, characterized by different hydrogen bonding patterns They are each compatible with their characteristic sets of main chain dihedral angles The part ...
... Recurring patterns of main chain conformations stabilized by hydrogen bonds There are two types of secondary structure, i.e. alpha-helix and beta-sheet, characterized by different hydrogen bonding patterns They are each compatible with their characteristic sets of main chain dihedral angles The part ...
IN MEDICAI, RIOCHHMISTIT UNIVERSITY OF CALICUT
... There shall be University examinations at the end of each sernester as detailed in the scheme of examination, making up a total of three- first at the end of 1"r semester, secbnd at the end of 2nd iemester and third at the end of 4rh semester [i.e., for third and fourth semesters combined]. There sh ...
... There shall be University examinations at the end of each sernester as detailed in the scheme of examination, making up a total of three- first at the end of 1"r semester, secbnd at the end of 2nd iemester and third at the end of 4rh semester [i.e., for third and fourth semesters combined]. There sh ...
Antibody to Myosin Heavy Chain (fast)
... intended for immunohistochemistry methodologies. A differential diagnosis is complemented by using a panel of antibodies and in conjunction with morphological studies, patient clinical history and other diagnostic tests by a qualified pathologist. Myosin is a contractile muscle specific protein comp ...
... intended for immunohistochemistry methodologies. A differential diagnosis is complemented by using a panel of antibodies and in conjunction with morphological studies, patient clinical history and other diagnostic tests by a qualified pathologist. Myosin is a contractile muscle specific protein comp ...
Single-stranded DNA-binding Proteins
... dsDNA, dsRNA and single-stranded RNA (ssRNA) share many of these properties. How does an ssDNA-binding protein exclude these competing molecules? The exclusion of double-stranded nucleic acids is relatively simple. Although dsDNA and dsRNA both have an electronegative phosphodiester backbone, they a ...
... dsDNA, dsRNA and single-stranded RNA (ssRNA) share many of these properties. How does an ssDNA-binding protein exclude these competing molecules? The exclusion of double-stranded nucleic acids is relatively simple. Although dsDNA and dsRNA both have an electronegative phosphodiester backbone, they a ...
Urea cycle
... liver, dissolved in blood (in a concentration of 2.5 - 7.5 mM), and secreted by the kidney. Urea also plays a very important role in protein catabolism, removal of toxic ammonia from the body. Urea determination is very useful for the medical clinician to assess kidney and other organs function ...
... liver, dissolved in blood (in a concentration of 2.5 - 7.5 mM), and secreted by the kidney. Urea also plays a very important role in protein catabolism, removal of toxic ammonia from the body. Urea determination is very useful for the medical clinician to assess kidney and other organs function ...
A Novel Role for Vitamin K1 in a Tyrosine Phosphorylation
... Tris–HCI (pH 7.4), and twice with 10 mM Tris–HCl (pH 7.4). The ...
... Tris–HCI (pH 7.4), and twice with 10 mM Tris–HCl (pH 7.4). The ...
translation
... 1. After the ribosome has move about 75 bases down the RNA from the ShineDelgaro or cap-binding proteins, these are free to bind another ribosome 2. A mRNA normally contain many ribosomes at a given time a) The protein attached to the ribosome are longer the closer the ribosome is to the 3' end of t ...
... 1. After the ribosome has move about 75 bases down the RNA from the ShineDelgaro or cap-binding proteins, these are free to bind another ribosome 2. A mRNA normally contain many ribosomes at a given time a) The protein attached to the ribosome are longer the closer the ribosome is to the 3' end of t ...
Interactions of liposomes and lipid-based carrier systems with blood
... used for the delivery of relatively large DNA and RNA-based drugs, including plasmids, antisense oligonucleotides and ribozymes. Despite recent successes in prolonging the circulation times of liposomes, virtually all lipid compositions studied to date are removed from the plasma compartment within ...
... used for the delivery of relatively large DNA and RNA-based drugs, including plasmids, antisense oligonucleotides and ribozymes. Despite recent successes in prolonging the circulation times of liposomes, virtually all lipid compositions studied to date are removed from the plasma compartment within ...
Gene encoding the group B streptococcal protein R4, its
... prototypic reference antisera for R4 in double-diffusion (results not shown). The trypsin-extracted R4 showed a precipitin result with anti-R4 antiserum for both controls. As the classic example of pepsin sensitivity for pepsin at pH2, no precipitin result was shown. At pH4, pH6 and pH8, however, pr ...
... prototypic reference antisera for R4 in double-diffusion (results not shown). The trypsin-extracted R4 showed a precipitin result with anti-R4 antiserum for both controls. As the classic example of pepsin sensitivity for pepsin at pH2, no precipitin result was shown. At pH4, pH6 and pH8, however, pr ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.